Control of arbovirus infection by Insect-specific virus

• Project/Area Number: 20K21298
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 39:Agricultural and environmental biology and related fields
• Research Institution: Hokkaido University
• Principal Investigator: Orba Yasuko 北海道大学, 人獣共通感染症国際共同研究所, 准教授 (60507169)
• Project Period (FY): 2020-07-30 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2022: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2021: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 蚊媒介性ウイルス / 昆虫特異的ウイルス / 蚊 / アルボウイルス / ヒトスジシマカ

Outline of Research at the Start

本研究は、蚊が媒介しヒトや動物に感染症を起こすアルボウイルスの増殖において、蚊と共存し病原性の無い昆虫特異的ウイルスが及ぼす影響を明らかにし、媒介蚊におけるウイルスによるウイルス制御の可能性を示すことを目的とする。自然界に生息する媒介蚊において、昆虫特異的ウイルスが共存することにより、アルボウイルス媒介能が低下することを明らかにし、昆虫特異的ウイルス感染蚊が、デングウイルスやジカウイルス等のアルボウイルス感染症の蔓延を阻止する可能性を提唱する。

Outline of Final Research Achievements

The aim of this study is to clarify the effects of non-pathogenic insect-specific viruses that co-exist with mosquitoes on replication and transmission when mosquitoes are simultaneously infected with mosquito-borne viruses that cause infectious diseases.
Using two types of insect-specific viruses which we have isolated from mosquitoes, we investigated the effects of these viruses on the growth of dengue virus type 2 in cultured cells and adult mosquito vectors. The results indicated that the infection rate and viral replication of dengue virus 2 were not affected by co-infection with these insect-specific viruses in adult Aedes albopictus mosquitoes.

Academic Significance and Societal Importance of the Research Achievements

蚊などの節足動物が媒介しヒトや動物に感染症を起こすデング熱等のウイルス感染症の多くは、未だ予防法や治療法が確立しておらず、媒介蚊の対策が最も重要である。本研究は、媒介蚊に共存し病原性の無いウイルスの存在と性状を明らかにし、蚊の中でデングウイルス等の病原性ウイルスの増殖を抑制する方法を検証するものである。媒介蚊を自然界から排除するのは困難であり、蚊に感染する様々なウイルスの特性を理解し利用することで、環境に配慮した蚊媒介性ウイルス感染症の予防策を見出す研究である。

Research Products

• [Int'l Joint Research] Gabriel Rene Moreno Autonomous Univ.(ボリビア)
• [Int'l Joint Research] University of Zambia(ザンビア)
• [Int'l Joint Research] 国立アイルランド大学ダブリン校(アイルランド)
• [Int'l Joint Research] ザンビア大学獣医学部(ザンビア)
• [Int'l Joint Research] Gabriel Rene Moreno大学獣医学部(ボリビア)
• [Int'l Joint Research] Queensland大学(オーストラリア)
• [Int'l Joint Research] ウイスコンシン大学(米国)
• [Int'l Joint Research] 国立アイルランド大学ダブリン校(アイルランド)
• [Int'l Joint Research] ザンビア大学(ザンビア)
• [Int'l Joint Research] Gabriel Rene Moreno大学獣医学部(ボリビア)
• [Int'l Joint Research] Queensland大学(オーストラリア)
• [Journal Article] An unusually long Rift valley fever inter-epizootic period in Zambia: Evidence for enzootic virus circulation and risk for disease outbreak (2022)
  • Author(s): Chambaro Herman M.、Hirose Kazuyo、Sasaki Michihito、Libanda Brigadier、Sinkala Yona、Fandamu Paul、Muleya Walter、Banda Fredrick、Chizimu Joseph、Squarre David、Shawa Misheck、Qiu Yongjin、Harima Hayato、Eshita Yuki、Simulundu Edgar、Sawa Hirofumi、Orba Yasuko
  • Journal Title: PLOS Neglected Tropical Diseases Volume: 16 Issue: 6 Pages: e0010420-e0010420
  • DOI: 10.1371/journal.pntd.0010420
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Serological characterization of lineage II insect-specific flaviviruses compared with pathogenic mosquito-borne flaviviruses (2022)
  • Author(s): Tabata Koshiro、Itakura Yukari、Toba Shinsuke、Uemura Kentaro、Kishimoto Mai、Sasaki Michihito、Harrison Jessica J.、Sato Akihiko、Hall William W.、Hall Roy A.、Sawa Hirofumi、Orba Yasuko
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 616 Pages: 115-121
  • DOI: 10.1016/j.bbrc.2022.05.080
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Current knowledge of vector-borne zoonotic pathogens in Zambia: A clarion call to scaling-up "One Health" research in the wake of emerging and re-emerging infectious diseases. (2022)
  • Author(s): Mubemba B, Mburu MM, Changula K, Muleya W, Moonga LC, Chambaro HM, Kajihara M, Qiu Y, Orba Y, Hayashida K, Sutcliffe CG, Norris DE, Thuma PE, Ndubani P, Chitanga S, Sawa H, Takada A, Simulundu E.
  • Journal Title: PLoS Negl Trop Dis. Volume: 16(2) Issue: 2 Pages: e0010193-e0010193
  • DOI: 10.1371/journal.pntd.0010193
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mosquito-Borne Viral Pathogens Detected in Zambia: A Systematic Review. (2021)
  • Author(s): Velu RM, Kwenda G, Libonda L, Chisenga CC, Flavien BN, Chilyabanyama ON, Simunyandi M, Bosomprah S, Sande NC, Changula K, Muleya W, Mburu MM, Mubemba B, Chitanga S, Tembo J, Bates M, Kapata N, Orba Y, Kajihara M, Takada A, Sawa H, Chilengi R, Simulundu E.
  • Journal Title: Pathogens Volume: 10(8) Issue: 8 Pages: 1007-1007
  • DOI: 10.3390/pathogens10081007
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An African tick flavivirus forming an independent clade exhibits unique exoribonuclease-resistant RNA structures in the genomic 3′-untranslated region (2021)
  • Author(s): Harima Hayato、Orba Yasuko、Torii Shiho、Qiu Yongjin、Kajihara Masahiro、Eto Yoshiki、Matsuta Naoya、Hang’ombe Bernard M.、Eshita Yuki、Uemura Kentaro、Matsuno Keita、Sasaki Michihito、Yoshii Kentaro、Nakao Ryo、Hall William W.、Takada Ayato、Abe Takashi、Wolfinger Michael T.、Simuunza Martin、Sawa Hirofumi
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 4883-4883
  • DOI: 10.1038/s41598-021-84365-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Diverse mosquito-specific flaviviruses in the Bolivian Amazon basin (2021)
  • Author(s): Orba Yasuko、Matsuno Keita、Nakao Ryo、Kryukov Kirill、Saito Yumi、Kawamori Fumihiko、Loza Vega Ariel、Watanabe Tokiko、Maemura Tadashi、Sasaki Michihito、Hall William W.、Hall Roy A.、Pereira Juan Antonio、Nakagawa So、Sawa Hirofumi
  • Journal Title: Journal of General Virology Volume: 102 Issue: 3 Pages: 001518-001518
  • DOI: 10.1099/jgv.0.001518
  • NAID: 120007041994
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Lethal(2)-Essential-for-Life [L(2)EFL] Gene Family Modulates Dengue Virus Infection in Aedes aegypti (2020)
  • Author(s): Runtuwene Lucky R., Kawashima Shuichi et al.
  • Journal Title: International Journal of Molecular Sciences Volume: 21 Issue: 20 Pages: 7520-7520
  • DOI: 10.3390/ijms21207520
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Discoveries of Exoribonuclease-Resistant Structures of Insect-Specific Flaviviruses Isolated in Zambia. (2020)
  • Author(s): Wastika CE, Harima H, Sasaki M, Hang'ombe BM, Eshita Y, Qiu Y, Hall WW, Wolfinger MT, Sawa H, Orba Y.
  • Journal Title: Viruses Volume: 12(9) Issue: 9 Pages: 1017-1017
  • DOI: 10.3390/v12091017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Co-Circulation of Multiple Serotypes of Bluetongue Virus in Zambia. Viruses. (2020)
  • Author(s): Chambaro HM, Sasaki M, Simulundu E, Silwamba I, Sinkala Y, Gonzalez G, Squarre D, Fandamu P, Lubaba CH, Munyeme M, Maseko A, Chimvwele C, Mataa L, Mooya LE, Mukubesa AN, Harima H, Samui KL, Munang'andu HM, Simuunza M, Nalubamba KS, Qiu Y, Carr MJ, Hall WW, Eshita Y, Sawa H, Orba Y.
  • Journal Title: Viruses Volume: 12(9) Issue: 9 Pages: 963-963
  • DOI: 10.3390/v12090963
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Host ESCRT factors are recruited during chikungunya virus infection and are required for the intracellular viral replication cycle. (2020)
  • Author(s): Torii S, Orba Y, Sasaki M, Tabata K, Wada Y, Carr M, Hobson-Peters J, Hall RA, Takada A, Fukuhara T, Matsuura Y, Hall WW, Sawa H.
  • Journal Title: J Biol Chem Volume: 295(23) Issue: 23 Pages: 7941-7957
  • DOI: 10.1074/jbc.ra119.012303
  • NAID: 120007044516
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 節足動物媒介性ウイルス（アルボウイルス） (2020)
  • Author(s): 大場靖子、澤洋文、松野啓太
  • Journal Title: ウイルス Volume: 70 Pages: 3-14
  • NAID: 130008036180
• [Presentation] 継代飼育ヒトスジシマカに存在する昆虫特異的ウイルスの単離とアルボウイルス感染への影響 (2022)
  • Author(s): 大場 靖子、田畑 耕史郎、佐々木 道仁、澤 洋文、江下 優樹
  • Organizer: 第69回日本ウイルス学会学術集会
• [Presentation] 蚊特異的フラビウイルスの蚊成体における感染動態の解析 (2022)
  • Author(s): 田畑耕史郎、江下優樹、佐々木道仁、澤洋文、大場靖子
  • Organizer: 第69回日本ウイルス学会学術集会
• [Presentation] 地球規模で広がる蚊媒介ウイルス感染症の対策に向けて (2022)
  • Author(s): 大場 靖子
  • Organizer: 山口大学 中高温微生物研究センター発酵微生物部門シンポジウム「未来を拓くバイオSDGs」
  • Invited
• [Presentation] 蚊特異的フラビウイルス抗体の脊椎動物感染性フラビウイルスへの反応性解析 (2021)
  • Author(s): 田畑耕史郎、板倉友香里、岸本麻衣、佐々木道仁、澤洋文、大場靖子
  • Organizer: 第68回日本ウイルス学会学術集会
• [Book] ネオウイルス学 (2021)
  • Author(s): 河岡 義裕、岩見 真吾、大場 靖子、川口 寧、佐藤 佳、澤 洋文、鈴木 信弘、高橋 英樹、朝長 啓造、中川 草、長崎 慶三、西浦 博、野田 岳志、古瀬 祐気、堀江 真行、牧野 晶子、松浦 善治、松野 啓太、村田 和義、望月 智弘、渡辺 登喜子
  • Total Pages: 320
  • Publisher: 集英社
  • ISBN: 9784087211597