• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

A challenge to modify the vernalization requirement of root and leaf vegetables of Brassica

Research Project

Project/Area Number 20K21313
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 39:Agricultural and environmental biology and related fields
Research InstitutionKobe University

Principal Investigator

Fujimoto Ryo  神戸大学, 農学研究科, 准教授 (60620375)

Co-Investigator(Kenkyū-buntansha) 長部 謙二  大阪大学, 産業科学研究所, 助教 (60751352)
Project Period (FY) 2020-07-30 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2021: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywordsアブラナ科 / 春化 / 晩抽性 / エピジェネティクス
Outline of Research at the Start

アブラナ科葉根菜では、花を咲かせる為には一定期間の低温にさらされる必要がある。これらの野菜は、花芽を形成しとう立ちすると、商品価値が損なわれるため、低温要求性の大きい系統が望まれる。しかし一方で、品種育成過程においては、低温要求性は大きくなりすぎると育種の効率を妨げるといったジレンマが生じる。本研究では、ヒストンメチル化酵素の阻害剤とエピゲノム編集により本ジレンマを解消することを目的とする。

Outline of Final Research Achievements

This study aims to artificially control the low-temperature requirement of Brassica vegetables. In this study, we found four extremely late-bolting lines, one of which had an insertion in the first intron of the BrFLC2 or BrFLC3 gene. These inserted regions were highly DNA methylated. We suggest that DNA methylation in the inserted regions of these two genes are the possible cause of the decreased cold response. We also constructed a vector for epigenome editing using Arabidopsis thaliana to modify histone modifications at the FLC locus.

Academic Significance and Societal Importance of the Research Achievements

アブラナ科野菜において、低温要求性は品種開発において重要な形質である。栽培中には、低温により、とう立ちが起こると商品価値が損なわれるため、高い低温要求性を示す品種開発が求められている。しかし一方で、低温要求性を大きくすれば、花を咲かせるのに時間がかかり、品種開発の効率が悪くなる。本研究成果は低温要求性を人為的に改変することで、品種開発を効率的に行う技術開発へと発展する可能性が期待されることから、社会的な意義がある。

Report

(3 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • Research Products

    (6 results)

All 2022 2021 Other

All Int'l Joint Research (2 results) Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (1 results)

  • [Int'l Joint Research] CSIRO(オーストラリア)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] CSIRO(オーストラリア)

    • Related Report
      2020 Research-status Report
  • [Journal Article] Transcriptional Association between mRNAs and Their Paired Natural Antisense Transcripts Following Fusarium oxysporum Inoculation in Brassica rapa L.2021

    • Author(s)
      Mst. Arjina Akter, Hasan Mehraj, Naomi Miyaji, Satoshi Takahashi, Takeshi Takasaki-Yasuda, Motoaki Seki, Elizabeth S. Dennis, Ryo Fujimoto, Kenji Osabe
    • Journal Title

      Horticulturae

      Volume: 8 Issue: 1 Pages: 17-17

    • DOI

      10.3390/horticulturae8010017

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genome-wide analysis of long noncoding RNAs, 24-nt siRNAs, DNA methylation and H3K27me3 marks in Brassica rapa2021

    • Author(s)
      Mehraj H, Shea DJ, Takahashi S, Miyaji N, Akter A, Seki M, Dennis ES, Fujimoto R, Osabe K.
    • Journal Title

      PLoS One

      Volume: 16 Issue: 3 Pages: e0242530-e0242530

    • DOI

      10.1371/journal.pone.0242530

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genome Triplication Leads to Transcriptional Divergence of FLOWERING LOCUS C Genes During Vernalization in the Genus Brassica2021

    • Author(s)
      Akter A, Itabashi E, Kakizaki T, Okazaki K, Dennis ES, Fujimoto R.
    • Journal Title

      Front Plant Sci

      Volume: 11 Pages: 619417-619417

    • DOI

      10.3389/fpls.2020.619417

    • NAID

      120006975756

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Brassica rapaにおける極晩抽性系統の春化特性評価2022

    • Author(s)
      Akter Ayasha, 藤本龍
    • Organizer
      日本育種学会第141回講演会
    • Related Report
      2021 Annual Research Report

URL: 

Published: 2020-08-03   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi