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Expression of phenotype in the offspring controlled by parental mating preference

Research Project

Project/Area Number 20K21368
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 42:Veterinary medical science, animal science, and related fields
Research InstitutionShizuoka University

Principal Investigator

Sasanami Tomohiro  静岡大学, 農学部, 教授 (80322139)

Project Period (FY) 2020-07-30 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2021: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2020: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Keywordsウズラ / 精子 / 受精 / 精子鞭毛長 / 配偶者選択 / 受精能力 / 鞭毛 / 性選択 / 精子競争 / 精子選択 / 貯精
Outline of Research at the Start

生殖活動は次世代に自身の子孫を残す為に必須であり、生物にとっての最重要課題である。加えて、優秀な形質を有した子孫を残す為には、優秀なパートナーを選ぶことが重要である。雄の個体の男性ホルモン濃度は、その雄個体の質を反映するという仮説が種々の動物で提唱されている。しかし、雌に好まれる雄の実際の受精能力や、その子孫の形質の調査はほとんど行われていない。本研究では、雌に好まれる雄と好まれない雄の受精能力およびその子孫の形質を調べ、生物の進化と生殖システムとの関連を明らかにするとともに、得られた知見を優秀な家禽・家畜の生産へとフィードバックが可能な、きわめて挑戦的で萌芽性の高い研究を遂行する。

Outline of Final Research Achievements

Reproduction is essential for the generation of offspring in the next generation. In this study, I investigated the actual fertilization ability of males preferred by females and the traits of their offspring. Comparing the fertilization ability of males preferred by females and males not preferred by females, it was found that males with longer sperm flagellum length had an advantage in fertilization, regardless of the testosterone concentration in their blood. In addition, F2 generation was produced from the quail line that produced sperm with long flagella and another quail line that produced sperm with short flagella, and both were examined for flagellar length and QTLseq analysis of genomic DNA was performed. The results showed that sperm flagellar length is inherited from one generation to the next and is not affected by environmental factors. In other words, the fertilization ability of males preferred by females was not necessarily high.

Academic Significance and Societal Importance of the Research Achievements

雄の個体の血中男性ホルモン濃度は、その雄個体の質を反映するという仮説が種々の動物で提唱されてきた。しかし、雌に好まれる雄の実際の受精能力や、その子孫の形質の調査はほとんど行われてこなかった。本研究結果は、この仮説は個体の繁殖能力に対しては正しいと言えるが、次世代の形質に関しては必ずしも正しくないことを示唆するものである。このことは、優秀な家畜・家禽を作出するために重要な雄の形質を調べる上で、精子の運動性や受精能力以外にも注目しなければならないことを意味する。

Report

(3 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • Research Products

    (14 results)

All 2022 2021 2020

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 5 results) Presentation (7 results)

  • [Journal Article] Effect of Anserine and Carnosine on Sperm Motility in the Japanese Quail2021

    • Author(s)
      Sarkar Prodip Kumar、Egusa Ai、Matsuzaki Mei、Sasanami Tomohiro
    • Journal Title

      The Journal of Poultry Science

      Volume: 58 Issue: 3 Pages: 186-191

    • DOI

      10.2141/jpsa.0200071

    • NAID

      130008067580

    • ISSN
      1346-7395, 1349-0486
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Longer and faster sperm exhibit better fertilization success in Japanese quail.2021

    • Author(s)
      Matsuzaki M, Hirohashi N, Tsudzuki M, Haqani MI, Maeda T, Mizushima S, Sasanami T.
    • Journal Title

      Poultry Science

      Volume: 100 Issue: 4 Pages: 100980-100980

    • DOI

      10.1016/j.psj.2021.01.003

    • Related Report
      2021 Annual Research Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of sperm surface oligosaccharides in sperm passage into sperm storage tubules in Japanese quail (Coturnix japonica).2021

    • Author(s)
      Matsuzaki M, Hirohashi N, Mizushima S, Sasanami T.
    • Journal Title

      Animal Reproduction Science

      Volume: 227 Pages: 106731-106731

    • DOI

      10.1016/j.anireprosci.2021.106731

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cyclin D1 gene expression is essential for cell cycle progression from the maternal-to-zygotic transition during blastoderm development in Japanese quail2021

    • Author(s)
      Mizushima, S., Sasanami, T., Ono, T., Kansaku, N., Matsuzaki, M., and Kuroiwa, A.
    • Journal Title

      Developmental Biology

      Volume: 476 Pages: 249-258

    • DOI

      10.1016/j.ydbio.2021.04.005

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Monitoring the reactive oxygen species in spermatozoa during liquid storage of boar semen and its correlation with sperm motility, free thiol content and seasonality2021

    • Author(s)
      Khoi Hoang Xuan、Shimizu Kenji、Yoneda Yoshitaka、Minagawa Itaru、Abe Yasuyuki、Kuwabara Yasushi、Sasanami Tomohiro、Kohsaka Tetsuya
    • Journal Title

      Andrologia

      Volume: 53 Issue: 11 Pages: 1-8

    • DOI

      10.1111/and.14237

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rare polyandry and common monogamy in the firefly squid, Watasenia scintillans2020

    • Author(s)
      Sato Noriyosi、Tsuda Sei-Ichiro、Nur E. Alam Md.、Sasanami Tomohiro、Iwata Yoko、Kusama Satoshi、Inamura Osamu、Yoshida Masa-aki、Hirohashi Noritaka
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 10962-10962

    • DOI

      10.1038/s41598-020-68006-1

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] An efficient heterologous Escherichia coli-based expression system for lectin production from Pleurocybella porrigens2020

    • Author(s)
      Suzuki Tomohiro、Nakamura Luna、Inayoshi Satomi、Tezuka Yuki、Ono Akiko、Choi Jae-Hoon、Dohra Hideo、Sasanami Tomohiro、Hirai Hirofumi、Kawagishi Hirokazu
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 85 Issue: 3 Pages: 630-633

    • DOI

      10.1093/bbb/zbaa058

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] ウズラの精子表面で精子貯蔵管への精子侵入に関与するタンパク質2022

    • Author(s)
      笹浪知宏・松崎芽衣・水島秀成
    • Organizer
      日本家禽学会2022年度春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 精子貯蔵管への精子侵入を阻害するタンパク質2021

    • Author(s)
      笹浪知宏・松崎芽衣・高塚夢々・水島秀成
    • Organizer
      第45回鳥類内分泌研究会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ウズラDAZL(deleted in azoospermia-like)mRNAの3’-UTRに結合するタンパク質の解析2021

    • Author(s)
      水島秀成・塚田光・笹浪知宏・小野珠乙・黒岩麻里
    • Organizer
      第45回鳥類内分泌研究会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ウズラの精子-卵子相互作用におけるAnnexin6の役割2021

    • Author(s)
      市川 佳伸、松崎芽衣、水島秀成、笹浪知宏
    • Organizer
      日本家禽学会2021年度春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] ウズラ精子表面の糖鎖が精子貯蔵管への精子侵入へ果たす役割2021

    • Author(s)
      松崎 芽衣、水島秀成、広橋教貴、堀内浩幸、笹浪知宏
    • Organizer
      日本家禽学会2021年度春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] ウズラの性比に影響を及ぼす要因2020

    • Author(s)
      笹浪知宏、青島優香、松崎芽衣、水島秀成
    • Organizer
      第44 回鳥類内分泌研究会
    • Related Report
      2020 Research-status Report
  • [Presentation] ウズラ初期胚におけるDAZL(deleted in azoospermia-like)の発現解析2020

    • Author(s)
      水島秀成、塚田光、笹浪知宏、小野珠乙、黒岩麻里
    • Organizer
      第44 回鳥類内分泌研究会
    • Related Report
      2020 Research-status Report

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Published: 2020-08-03   Modified: 2023-01-30  

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