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A strategy to elucidate unknown functions of plant secreted peptides.

Research Project

Project/Area Number 20K21422
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
Research InstitutionNagoya University

Principal Investigator

Uchida Naoyuki  名古屋大学, 遺伝子実験施設, 教授 (40467692)

Project Period (FY) 2020-07-30 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2021: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords分泌ペプチド / 変異体 / 環境応答 / シグナルセンター
Outline of Research at the Start

多細胞生物の発生や環境応答などに関わる内在性の新シグナル分子が1つ発見され、その機能に迫る最初のきっかけさえ報告されると、それを皮切りにその関連事象の解明が世界中で始まる。新重要シグナルの発見は、往々にしてそれ以前までの制御モデルの改訂や大きな見直しにつながることから、その分野の発展の大きな節目となるなど、新シグナル分子の機能の発見1つ1つが新しい研究展開・研究転換の萌芽的起点となる。しかし、その最初のきっかけの発見が最も難しい壁である。そこで本研究では、独自に整備する変異体ライブラリーを活用することで、機能未知の植物のペプチド性の分泌シグナル群の機能解明を行う。

Outline of Final Research Achievements

In this study, we aimed to identify secreted peptides with novel functions in plants and determine their functions and expression patterns. We also studied the dependence of their actions on environmental changes. We selected approximately 80 peptide genes presumed to show cell type-specific expression patterns using various databases and developed a collection of their mutants. We generated high-order-multiple mutants when redundant genes seemed present in the genome. These mutants were grown under several conditions and screened for their phenotypes. We also analyzed the expression patterns of the selected factors that exhibited interesting phenotypes. As a result, we identified several secreted peptides that do not show apparent mutant phenotypes under normal conditions but exhibit interesting phenotypes only when the mutants are exposed to stresses related to their expression control.

Academic Significance and Societal Importance of the Research Achievements

多細胞生物の発生や環境応答などに関わる内在性の新重要シグナルの発見は、それ以前までの制御モデルの改訂や大きな見直しにつながることから、その分野の発展の大きな節目となることが多く、新シグナル分子の機能の発見1つ1つが新しい研究展開・研究転換の萌芽的起点となる。とりわけ、環境応答に関わるシグナル経路は、気候変動などにより変動する環境下でも生存できる植物種や農作品種の創出にも貢献できる可能性もあり、今後の活用が期待される。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (7 results)

All 2023 2020 Other

All Int'l Joint Research (1 results) Journal Article (5 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (1 results)

  • [Int'l Joint Research] テキサス大学(米国)

    • Related Report
      2022 Annual Research Report
  • [Journal Article] Identification of a pluripotency-inducing small compound, PLU, that induces callus formation via Heat Shock Protein 90-mediated activation of auxin signaling.2023

    • Author(s)
      Nakashima Y, Kobayashi Y, Murao M, Kato R, Endo H, Higo A, Iwasaki R, Kojima M, Takebayashi Y, Sato A, Nomoto M, Sakakibara H, Tada Y, Itami K, Kimura S, Hagihara S, Torii KU, Uchida N
    • Journal Title

      Front. Plant Sci.

      Volume: 14 Pages: 1099587-1099587

    • DOI

      10.3389/fpls.2023.1099587

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] EPFL peptide signaling ensures robust self‐pollination success under cool temperature stress by aligning the length of the stamen and pistil.2023

    • Author(s)
      Negoro S, Hirabayashi T, Iwasaki R, Torii KU, Uchida N
    • Journal Title

      Plant Cell Environ.

      Volume: 46 Issue: 2 Pages: 451-463

    • DOI

      10.1111/pce.14498

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A peptide pair coordinates regular ovule initiation patterns with seed number and fruit size.2020

    • Author(s)
      Kawamoto N, Pino Del Carpio D, Hofmann A, Mizuta Y, Kurihara D, Higashiyama T, Uchida N, Torii KU, Colombo L, Groth G, Simon R
    • Journal Title

      Curr. Biol.

      Volume: 30 Issue: 22 Pages: 4352-4361

    • DOI

      10.1016/j.cub.2020.08.050

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Induction of Multichotomous Branching by CLAVATA Peptide in Marchantia polymorpha2020

    • Author(s)
      Hirakawa Yuki、Fujimoto Toko、Ishida Sakiko、Uchida Naoyuki、Sawa Shinichiro、Kiyosue Tomohiro、Ishizaki Kimitsune、Nishihama Ryuichi、Kohchi Takayuki、Bowman John L.
    • Journal Title

      Current Biology

      Volume: 30 Issue: 19 Pages: 3833-3840

    • DOI

      10.1016/j.cub.2020.07.016

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair.2020

    • Author(s)
      Kohei Nishimura , Ryotaro Yamada , Shinya Hagihara , Rie Iwasaki , Naoyuki Uchida , Takumi Kamura , Koji Takahashi , Keiko U Torii , Tatsuo Fukagawa
    • Journal Title

      Nucleic acids research

      Volume: 48 Issue: 18 Pages: e108-e108

    • DOI

      10.1093/nar/gkaa748

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Manipulation of plant hormone responses and its application to rapid depletion of target proteins in animal cells2020

    • Author(s)
      Uchida N
    • Organizer
      第43回日本分子生物学会年会 シンポジウム「Accelerating life sciences and drug discovery through whole-animal chemical biology」
    • Related Report
      2020 Research-status Report

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Published: 2020-08-03   Modified: 2024-01-30  

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