Identification and characterization of novel microRNAs secreted from muscle
| Project/Area Number |
20K21753
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 59:Sports sciences, physical education, health sciences, and related fields
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| Research Institution | Nagoya University |
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Principal Investigator |
Ouchi Noriyuki 名古屋大学, 医学系研究科, 特任教授 (00595514)
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| Co-Investigator(Kenkyū-buntansha) |
大橋 浩二 名古屋大学, 医学系研究科, 特任准教授 (10595515)
竹藤 幹人 名古屋大学, 医学部附属病院, 講師 (20709117)
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| Project Period (FY) |
2020-07-30 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2021: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 持久性運動 / microRNA / 心腎血管疾患 / 運動 / 骨格筋 |
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| Outline of Research at the Start |
本研究では、持久性運動により調節を受ける骨格筋由来の分泌型microRNAのスクリーニングを行い、心血管病あるいは腎臓病に対して作用を有する骨格筋由来分泌型microRNAの同定を試みる。そして、本研究で明らかとなる骨格筋由来分泌型microRNAの機能解析を行うことで、運動療法による心腎血管保護作用の機序解明と心血管病や腎臓病の病態解明と創薬開発につなげる。
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| Outline of Final Research Achievements |
We found that miR185 acts as an exercise-inducible muscle-derived secretory microRNA “myomiRNA” in mice after screening of myomiRNAs which are upregulated in skeletal muscle by treadmill exercise training. Systemic delivery of miR185 mimic significantly reduced renal fibrosis in a mouse model of unilateral ureteral obstruction. Furthermore, miR185 suppressed expression of fibrotic markers in renal proximal tubular epithelial cells. Thus, miR185 may be a reno-protecitve secretory myomiRNA induced by endurance exercise.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、持久性運動により誘発され、腎臓保護作用を示す新たな骨格筋由来分泌型microRNAが明らかとなった。本研究は慢性腎臓病の病態解明につながる可能性があるだけではなく、この骨格筋由来分泌型microRNAは腎臓以外の臓器に対する作用を示す可能性もあり、学術的意義は高いと考えられる。また、この骨格筋由来分泌型microRNAは運動による腎臓保護作用にも関わるため、運動すれば何故健康に有益なのか？という疑問に対する解決の糸口になり得る可能性があり、社会的意義は高いと考えられる。
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Important Role of Concomitant Lymphangiogenesis for Reparative Angiogenesis in Hindlimb Ischemia.2021
Author(s)
Pu Z, Shimizu Y, Tsuzuki K, Suzuki J, Hayashida R, Kondo K, Fujikawa Y, Unno K, Ohashi K, Takefuji M, Bando YK, Ouchi N, Calvert JW, Shibata R, Murohara T.
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Journal Title
Arterioscler Thromb Vasc Biol.
Volume: -
Issue: 6
Pages: 2006-2018
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A novel selective PPARα modulator, pemafibrate promotes ischemia-induced revascularization through the eNOS-dependent mechanisms.2020
Author(s)
Kawanishi H, Ohashi K, Ogawa H, Otaka N, Takikawa T, Fang L, Ozaki Y, Takefuji M, Murohara T, Ouchi N.
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Journal Title
PLoS One.
Volume: 15
Issue: 6
Pages: e0235362-e0235362
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Adipolin/C1q/Tnf-related protein 12 prevents adverse cardiac remodeling after myocardial infarction.2020
Author(s)
Takikawa T, Ohashi K, Ogawa H, Otaka N, Kawanishi H, Fang L, Ozaki Y, Eguchi S, Tatsumi M, Takefuji M, Murohara T, Ouchi N.
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Journal Title
PLoS One.
Volume: 15
Issue: 12
Pages: e0243483-e0243483
DOI
Related Report
Peer Reviewed / Open Access
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