• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Individual fluid sampling and isolation of bacteria in fluid inclusion

Research Project

Project/Area Number 20K21839
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 63:Environmental analyses and evaluation and related fields
Research InstitutionTohoku University

Principal Investigator

Tsuchiya Noriyoshi  東北大学, 環境科学研究科, 教授 (40207410)

Co-Investigator(Kenkyū-buntansha) 平野 伸夫  東北大学, 環境科学研究科, 助教 (80344688)
Project Period (FY) 2020-07-30 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2021: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2020: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Keywords地下生物圏 / 流体包有物 / 海底噴気堆積鉱床 / 微生物隔離 / ラマン分光計測 / 地熱エネルギー / 微生物 / 黒鉱 / ラマン分光 / アルブミン / 深海底生物 / 流体採取 / レーザーアブレーション / PCR
Outline of Research at the Start

地下生物圏には,多様な生態系環境が存在していると推定されているが,地下生物圏の解析手法は未だ充分には開拓されていない.
岩石中には流体が含まれており(流体包有物),この中の微生物の生態情報を解析できれば,地下生物圏探索は大きく前進する.本研究は,岩石内に微生物含有人工流体包有物を合成し,その流体包有物の特徴情報や位置情報を失うことなく流体を個別に採取し,地下生物圏の微生物ゲノム解析技術の端緒を切り開く.

Outline of Final Research Achievements

We succeeded in synthesizing fluid inclusions artificially by encapsulating albumin solution in quartz, and then conducted Raman spectroscopy of these fluid inclusions to obtain spectra derived from C-H bonds. Based on these results, we attempted Raman spectroscopy of fluid inclusions in black ore samples from a natural black ore deposit (Ainai Mine, Akita Prefecture), and obtained spectra derived from C-H bonds similar to those of albumin, indicating that the black ore contains organic matter. Although we have not yet obtained proof of the presence of microorganisms, we believe that this method can be applied to the search for the presence of microorganisms associated with marine hydrothermal sedimentary deposits (VMS: Volcano hosted Massive Sulfides) and the underground biosphere.

Academic Significance and Societal Importance of the Research Achievements

地圏環境にはまだ未発見の生物が隠されており,それらは岩石・鉱石中の流体包有物中に隔離されていると推定される.この生物痕を調査するために,流体包有物を個別にラマン計測する方法を考案し,海底噴気堆積鉱床の黒鉱中の流体包有物に有機物が含まれることをはじめて認めた.まだ生物であるかどうかの確証は得られていないが,この方法を展開することにより未知の生物の探索につながると期待される.

Report

(3 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • Research Products

    (10 results)

All 2022 2021 2020

All Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 4 results) Presentation (3 results)

  • [Journal Article] Continental arc-derived eclogite in the Zavkhan Terrane, western Mongolia: Implications for the suture zone in the northern part of the Central Asian Orogenic Belt2022

    • Author(s)
      Bayarbold Manzshir、Okamoto Atsushi、Dandar Otgonbayar、Uno Masaoki、Tsuchiya Noriyoshi
    • Journal Title

      Journal of Asian Earth Sciences

      Volume: 229 Pages: 105150-105150

    • DOI

      10.1016/j.jseaes.2022.105150

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Multi-stage serpentinization of ultramafic rocks in the Manlay Ophiolite, southern Mongolia2021

    • Author(s)
      Nomuulin Amarbayar、Tsuchiya Noriyoshi、Dandar Otgonbayar、Okamoto Atsushi、Uno Masaoki、Batsaikhan Undarmaa、Wang Jiajie
    • Journal Title

      Mongolian Geoscientist

      Volume: 26 Issue: 53 Pages: 1-17

    • DOI

      10.5564/mgs.v26i53.1787

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Silica controls on hydration kinetics during serpentinization of olivine: Insights from hydrothermal experiments and a reactive transport model2020

    • Author(s)
      Oyanagi Ryosuke、Okamoto Atsushi、Tsuchiya Noriyoshi
    • Journal Title

      Geochimica et Cosmochimica Acta

      Volume: 270 Pages: 21-42

    • DOI

      10.1016/j.gca.2019.11.017

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rapid fluid infiltration and permeability enhancement during middle-lower crustal fracturing: Evidence from amphibolite-granulite-facies fluid-rock reaction zones, Sor Rondane Mountains, East Antarctica2020

    • Author(s)
      Diana Mindaleva, Masaoki Uno, Fumiko Higashino, Takayoshi Nagaya, Atsushi Okamoto, Noriyoshi Tsuchiya
    • Journal Title

      Lithos

      Volume: - Pages: 105521-105521

    • DOI

      10.1016/j.lithos.2020.105521

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transport and Evolution of Supercritical Fluids During the Formation of the Erdenet Cu?Mo Deposit, Mongolia2020

    • Author(s)
      Agroli Geri、Okamoto Atsushi、Uno Masaoki、Tsuchiya Noriyoshi
    • Journal Title

      Geosciences

      Volume: 10 Issue: 5 Pages: 201-201

    • DOI

      10.3390/geosciences10050201

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A geochemical approach for identifying marine incursions: Implications for tsunami geology on the Pacific coast of northeast Japan2020

    • Author(s)
      Takahiro Watanabe, Noriyoshi Tsuchiya, Shin-ichi Yamasaki, Yuki Sawai, Norihiro Hosoda, Fumiko W. Nara, Toshio Nakamura, Takeshi Komai
    • Journal Title

      Applied Geochemistry

      Volume: 118 Pages: 104644-104644

    • DOI

      10.1016/j.apgeochem.2020.104644

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fluid Infiltration Through Oceanic Lower Crust in Response to Reaction Induce d Fracturing: Insights From Serpentinized Troctolite and Numerical Models2020

    • Author(s)
      Kazuki Yoshida, Atsushi Okamoto, Hiroyuki Shimizu, Ryosuke Oyanagi,Noriyoshi Tsuchiya, and Oman Drilling Project Phase Science Party
    • Journal Title

      Journal of Geophygical Research

      Volume: ー Issue: 11

    • DOI

      10.1029/2020jb020268

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 流体包有物の合成とタンパク質の検出に関する基礎的検討2021

    • Author(s)
      陳 嘯天、WANG JIAJIE、平野 伸夫、土屋 範芳
    • Organizer
      日本鉱物科学会(JAMS)
    • Related Report
      2021 Annual Research Report
  • [Presentation] タンパク質の流体包有物封入実験と地熱環境に生息する細菌検出基礎的検討2021

    • Author(s)
      陳 嘯天、WANG JIAJIE、平野 伸夫、土屋 範芳
    • Organizer
      日本地熱学会(GRSJ)
    • Related Report
      2021 Annual Research Report
  • [Presentation] 流体のFlashingと岩石破壊2020

    • Author(s)
      土屋範芳
    • Organizer
      日本鉱物科学会
    • Related Report
      2020 Research-status Report

URL: 

Published: 2020-08-03   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi