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Genomic analysis of DMSP lyase-like genes in scleractinian corals

Research Project

Project/Area Number 20K21860
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 64:Environmental conservation measure and related fields
Research InstitutionThe University of Tokyo

Principal Investigator

Shinzato Chuya  東京大学, 大気海洋研究所, 准教授 (70524726)

Co-Investigator(Kenkyū-buntansha) 山下 洋  国立研究開発法人水産研究・教育機構, 水産技術研究所(長崎), 主任研究員 (00583147)
鈴木 豪  国立研究開発法人水産研究・教育機構, 水産技術研究所(長崎), 主任研究員 (30533319)
亀山 宗彦  北海道大学, 地球環境科学研究院, 准教授 (70510543)
Project Period (FY) 2020-07-30 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2022: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywordsサンゴ / DMSP / DMS / 遺伝子
Outline of Research at the Start

海岸を歩いていると感じる「磯の香り」硫化ジメチル(DMS)は、植物プランクトンの浸透圧調節物質であるDMSPから、DMSPリアーゼという酵素により生成される。DMSは大気中で水蒸気の凝結核となって雲を形成し、太陽光を遮ることで、地球冷却効果に重要な役割を果たすと考えられている。我々は18種の造礁サンゴの全ゲノムの比較解析から、通常動物に存在しないはずのDMSPリアーゼ酵素遺伝子が、サンゴ礁に優先しているミドリイシ属サンゴで、爆発的に増加していることを発見した。ミドリイシ属サンゴは、DMSを産出しているのだろうか?その可能性を遺伝子解析を中心に検証する。

Outline of Final Research Achievements

Dimethlysulfoniopropionate (DMSP) functions in osmoregulation and oxidative stress protection, etc. in phytoplankton and algae, and DMSP lyase mediates cleavage of DMSP into acrylate and dimethyl sulfide (DMS). DMS is a volatile substance that activates cloud formation, reducing solar radiation and ocean temperatures and contributing to atmosphere-ocean feedback. Thus, DMSP lyases in marine organisms may influence local climate. Although DMSP lyases were first identified in marine bacteria, they or genes similar to them, DMSP lyase-like, have been found in eukaryotes, not only in unicellular phytoplanktons, but also in scleractinian corals. Interestingly, DMSP lyase-like genes are the most diverse gene family in Acropora genomes and gene expansions have occurred specifically in the genus Acropora. In this project, we investigated the evolutionary background of DMSP lyase-like genes in the animal kingdom and eukaryotes and elucidated the possible function of these genes in corals.

Academic Significance and Societal Importance of the Research Achievements

DMSPリアーゼ様遺伝子はサンゴの共生藻からも確認されたので、当初はサンゴのDMSPリアーゼ様遺伝子は共生藻からの遺伝子水平伝播が疑われた。現在利用可能な遺伝子データベースを網羅的に解析したところ、共生藻からの水平伝播ではなく、刺胞動物の共通祖先でDMSPリアーゼ様遺伝子を獲得したことが示唆された。その他に、刺胞動物以外の動物からは確認されない、動物のDMSPリアーゼ様遺伝子は他の真核生物のDMSPリアーゼ様遺伝子や原核生物のDMSPリアーゼとは起源が異なる、ことも明らかにした。DMSPリアーゼ様遺伝子がサンゴにおいてどのような役割を果たしているのか、その詳細の解明が今後期待される。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (23 results)

All 2024 2023 2022 2021 2020

All Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 10 results) Presentation (13 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results)

  • [Journal Article] Genomic Data Reveal Diverse Biological Characteristics of Scleractinian Corals and Promote Effective Coral Reef Conservation2024

    • Author(s)
      Shinzato Chuya、Yoshioka Yuki
    • Journal Title

      Genome Biology and Evolution

      Volume: 16 Issue: 2 Pages: 1-8

    • DOI

      10.1093/gbe/evae014

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genes possibly related to symbiosis in early life stages of Acropora tenuis inoculated with Symbiodinium microadriaticum2023

    • Author(s)
      Yoshioka Yuki、Chiu Yi-Ling、Uchida Taiga、Yamashita Hiroshi、Suzuki Go、Shinzato Chuya
    • Journal Title

      Communications Biology

      Volume: 6 Issue: 1 Pages: 1-11

    • DOI

      10.1038/s42003-023-05350-8

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genomic and transcriptomic analyses illuminate the molecular basis of the unique lifestyle of a tubeworm, <i>Lamellibrachia satsuma</i>2023

    • Author(s)
      Uchida Taiga、Yoshioka Yuki、Yoshida Yu、Fujie Manabu、Yamaki Ayuta、Sasaki Akira、Inoue Koji、Shinzato Chuya
    • Journal Title

      DNA Research

      Volume: 30 Issue: 4

    • DOI

      10.1093/dnares/dsad014

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evolutionary History of DMSP Lyase-Like Genes in Animals and Their Possible Involvement in Evolution of the Scleractinian Coral Genus, Acropora2022

    • Author(s)
      Chiu Yi-Ling、Shinzato Chuya
    • Journal Title

      Frontiers in Marine Science

      Volume: 9

    • DOI

      10.3389/fmars.2022.889866

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparative genomics highlight the importance of lineage-specific gene families in evolutionary divergence of the coral genus, Montipora2022

    • Author(s)
      Yoshioka Yuki、Suzuki Go、Zayasu Yuna、Yamashita Hiroshi、Shinzato Chuya
    • Journal Title

      BMC Ecology and Evolution

      Volume: 22 Issue: 1 Pages: 71-71

    • DOI

      10.1186/s12862-022-02023-8

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genomic analysis of a reef‐building coral, <i>Acropora digitifera</i> , reveals complex population structure and a migration network in the Nansei Islands, Japan2022

    • Author(s)
      Tsuchiya Kojin、Zayasu Yuna、Nakajima Yuichi、Arakaki Nana、Suzuki Go、Satoh Noriyuki、Shinzato Chuya
    • Journal Title

      Molecular Ecology

      Volume: 31 Issue: 20 Pages: 5270-5284

    • DOI

      10.1111/mec.16665

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel Mitochondrial DNA Markers for Scleractinian Corals and Generic-Level Environmental DNA Metabarcoding2021

    • Author(s)
      Shinzato Chuya、Narisoko Haruhi、Nishitsuji Koki、Nagata Tomofumi、Satoh Noriyuki、Inoue Jun
    • Journal Title

      Frontiers in Marine Science

      Volume: 8 Pages: 1-9

    • DOI

      10.3389/fmars.2021.758207

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Whole-Genome Sequencing Highlights Conservative Genomic Strategies of a Stress-Tolerant, Long-Lived Scleractinian Coral, Porites australiensis Vaughan, 19182021

    • Author(s)
      Chuya Shinzato, Takeshi Takeuchi, Yuki Yoshioka, Ipputa Tada, Miyuki Kanda, Cedric Broussard, Akira Iguchi, Makoto Kusakabe, Frederic Marin, Noriyuki Satoh, Mayuri Inoue
    • Journal Title

      Genome Biology and Evolution

      Volume: 13 Issue: 12 Pages: 1-16

    • DOI

      10.1093/gbe/evab270

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Can Acropora tenuis larvae attract native Symbiodiniaceae cells by green fluorescence at the initial establishment of symbiosis?2021

    • Author(s)
      Yamashita H, Koike K, Shinzato C, Jimbo M, Suzuki G
    • Journal Title

      PLoS ONE

      Volume: 16 Issue: 6 Pages: e0252514-e0252514

    • DOI

      10.1371/journal.pone.0252514

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Eighteen Coral Genomes Reveal the Evolutionary Origin of Acropora Strategies to Accommodate Environmental Changes2020

    • Author(s)
      Shinzato Chuya、Khalturin Konstantin、Inoue Jun、Zayasu Yuna、Kanda Miyuki、Kawamitsu Mayumi、Yoshioka Yuki、Yamashita Hiroshi、Suzuki Go、Satoh Noriyuki
    • Journal Title

      Molecular Biology and Evolution

      Volume: 38 Issue: 1 Pages: 16-30

    • DOI

      10.1093/molbev/msaa216

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] シャコガイのゲノム解読と網羅的遺伝子発現解析による共生関連遺伝子の探索2024

    • Author(s)
      内田大賀, 島田剛,山下洋, 藤江学, 將口栄一, 新里宙也
    • Organizer
      日本水産学会春季大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ウスエダミドリイシの初期生活期における共生関連遺伝子2023

    • Author(s)
      善岡祐輝、Yi-Ling Chiu、内田大賀 、山下洋、鈴木豪、新里宙也
    • Organizer
      サンゴ礁学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Evolutionary strategy of the coral Montipora2023

    • Author(s)
      善岡祐輝、新里宙也
    • Organizer
      第25回日本進化学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 共生開始時におけるウスエダミドリイシの異なる褐虫藻株に対する遺伝子発現応答の網羅的比較2022

    • Author(s)
      善岡 祐輝,山下 洋,鈴木 豪,新里 宙也
    • Organizer
      日本サンゴ礁学会25回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Attempts to identify the possible heat-tolerance individuals of Acropora tenuis using SNP markers.2022

    • Author(s)
      Yi-Ling Chiu, Azusa Kinjo, Ryota Nakamura, Atsushi Shuto, Chuya Shinzato
    • Organizer
      日本サンゴ礁学会25回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] ナガレハナサンゴにおける蛍光タンパク質遺伝子の独自進化2022

    • Author(s)
      内田 大賀,識名 信也,新里 宙也
    • Organizer
      日本サンゴ礁学会25回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 南西諸島におけるコユビミドリイシ集団の複雑な分散ネットワークと歴史的変遷2022

    • Author(s)
      土屋 考人,座安 佑奈,中島 祐一,鈴木 豪,佐藤 矩行, 新里 宙也
    • Organizer
      日本サンゴ礁学会25回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] サンゴ-褐虫藻共生系の分子機構:異なる褐虫藻に対する宿主の遺伝子応答から探る2022

    • Author(s)
      善岡祐輝, 新里宙也
    • Organizer
      第81回日本寄生虫学会東日本支部大会・日本共生生物学会第6回大会 合同大会
    • Related Report
      2022 Research-status Report
  • [Presentation] サツマハオリムシのゲノム解析と組織別トランスクリプトーム解析による共生関連遺伝子の探索2022

    • Author(s)
      内田大賀, 善岡祐輝, 新里宙也
    • Organizer
      第81回日本寄生虫学会東日本支部大会・日本共生生物学会第6回大会 合同大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Marine Invertebrate Adaptations to Different Stressors: Genomic level2022

    • Author(s)
      Chuya Shinzato
    • Organizer
      ICGEB-USM-CREPSUM Course "Application of Advanced Genomic Tools for Conservation of Marine Biodiversity"
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] ハマサンゴ,その「コンサバ」なゲノム戦略2021

    • Author(s)
      新里宙也,竹内猛,善岡祐輝,多田一風太,神田美幸,井口亮,日下部誠,佐藤矩行,井上麻夕里
    • Organizer
      日本サンゴ礁学会24回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] Using genomics to reveal novel insights into coral biology2021

    • Author(s)
      Chuya Shinzato
    • Organizer
      Genome Concept Centennial Conference
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 18種のミドリイシ科サンゴの全ゲノム解読が明らかにする,ミドリイシ属の環境適応戦略2020

    • Author(s)
      新里宙也、Konstantin Khalturin 、井上潤、座安佑奈、神田美幸、川満真由美、善岡祐輝、山下洋、鈴木豪、佐藤矩行
    • Organizer
      日本サンゴ礁学会第23回大会
    • Related Report
      2020 Research-status Report

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Published: 2020-08-03   Modified: 2025-01-30  

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