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Development of liver and bile duct tissue engineering for bile excretion

Research Project

Project/Area Number 20K21908
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 90:Biomedical engineering and related fields
Research InstitutionKeio University

Principal Investigator

Sudo Ryo  慶應義塾大学, 理工学部(矢上), 教授 (20407141)

Project Period (FY) 2020-07-30 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2021: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords胆汁排泄 / 胆管 / 毛細胆管 / 共培養 / 三次元培養
Outline of Research at the Start

肝臓は生命維持に不可欠な機能を担うとともに、人工物で機能を代替することができないため、細胞培養による肝臓再生手法の開発が望まれている。従来研究では肝細胞の三次元培養に注力されてきたのに対して、本研究では、肝細胞による毛細胆管形成プロセスと胆管上皮細胞による胆管形成プロセスを時間的および空間的に制御することによって、毛細胆管と胆管を接合させる培養手法を確立し、胆汁排泄機能を有する肝・胆管複合組織の構築を目指す。

Outline of Final Research Achievements

In liver tissue engineering for liver regeneration in vitro, a culture method for constructing bile canaliculi by hepatocytes and a culture method for constructing bile ducts by biliary epithelial cells have been reported. However, a method for constructing liver tissue, in which bile canaliculi and bile ducts are directly connected, has not been established. In this study, the connection of bile canaliculi and bile ducts were induced in the coculture of primary rat hepatocytes and biliary epithelial cells by investigating the seeding concentration and timing in collagen gel sandwich culture. Furthermore, we demonstrated that bile canaliculi and bile ducts are directly connected using fluorescence imaging and immunocytochemistry. A new culture device was also developed to regulate the position where bile canaliculi were directly connected to bile ducts.

Academic Significance and Societal Importance of the Research Achievements

肝臓の組織工学研究において、毛細胆管や胆管をそれぞれ個別に再構築する培養法は報告されていたが、これらを融合した胆汁排泄を実現する培養法の確立は長い間重要な課題であった。本研究により毛細胆管と胆管が直接接合した肝組織を構築する手法が明らかになったため、胆汁排泄機能を有する肝組織の再生を実現した点で、本研究の成果は肝臓組織工学において極めて重要な学術的意義を有する。また、本研究の成果によって、今後は胆汁排泄という新たな観点から創薬研究・再生医療・バイオチップの研究が展開されることが期待される点で、社会的に重要な意義も有する。

Report

(3 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • Research Products

    (7 results)

All 2021 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results) Remarks (2 results)

  • [Journal Article] Generation of functional liver organoids on combining hepatocytes and cholangiocytes with hepatobiliary connections ex vivo2021

    • Author(s)
      Tanimizu Naoki、Ichinohe Norihisa、Sasaki Yasushi、Itoh Tohru、Sudo Ryo、Yamaguchi Tomoko、Katsuda Takeshi、Ninomiya Takafumi、Tokino Takashi、Ochiya Takahiro、Miyajima Atsushi、Mitaka Toshihiro
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 3390-3401

    • DOI

      10.1038/s41467-021-23575-1

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] ラット小型肝細胞と胆管上皮細胞を用いた毛細胆管―胆管接合培養法の検討2021

    • Author(s)
      金子翼、杉本葵、山下忠紘、谷水直樹、三高俊広、須藤亮
    • Organizer
      第28回肝細胞研究会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Development of an in vitro coculture model connecting bile canaliculi and bile ducts and its miniaturization2021

    • Author(s)
      Tsubasa Kaneko, Ryota Kaku, Tadahiro Yamashita, Ryo Sudo
    • Organizer
      TERMIS 6th World Congress
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ラット初代培養細胞を用いた毛細胆管-胆管接合組織の構築2021

    • Author(s)
      金子翼、杉本葵、山下忠紘、須藤亮
    • Organizer
      日本機械学会 第32回バイオフロンティア講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ラット初代培養細胞を用いた毛細胆管-胆管接合組織の構築と細胞配置の検討2021

    • Author(s)
      須藤亮、金子翼、杉本葵、山下忠紘、谷水直樹、三高俊広
    • Organizer
      第21回日本再生医療学会
    • Related Report
      2021 Annual Research Report
  • [Remarks] 慶應義塾大学理工学部システムデザイン工学科須藤研究室

    • URL

      http://www.sudo.sd.keio.ac.jp/index.html

    • Related Report
      2021 Annual Research Report
  • [Remarks] 慶應義塾大学理工学部システムデザイン工学科須藤研究室ホームページ

    • URL

      http://www.sudo.sd.keio.ac.jp/index.html

    • Related Report
      2020 Research-status Report

URL: 

Published: 2020-08-03   Modified: 2023-01-30  

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