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Physiological analysis of the resistant mechanisms of soybean for Spodotera litura

Research Project

Project/Area Number 20K22584
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeMulti-year Fund
Review Section 0602:Agricultural and environmental biology and related fields
Research InstitutionKyoto University of Advanced Science

Principal Investigator

Nakata Ryu  京都先端科学大学, バイオ環境学部, 講師 (90882548)

Project Period (FY) 2020-09-11 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsダイズ / ハスモンヨトウ / 抵抗性 / 作用機構 / プロテアーゼインヒビター / 抵抗性機構 / プロテアーゼ / 生理解析 / 消化酵素 / エネルギー代謝 / 代謝物
Outline of Research at the Start

ハスモンヨトウはダイズの主要な害虫である.高品質な強抵抗性のダイズ品種を開発するため,これまでにダイズが持つ遺伝子や代謝物が解析されてきたが,未だ達成されていない.本研究では抵抗性機構の解明を目指し,抵抗性ダイズ品種を加害したハスモンヨトウ幼虫の生理変化に着目する.そして,その原因となるダイズの抵抗性代謝物を明らかにする.抵抗性機構の分子レベルでの理解を深めることで,その知見に基づいた高品質な強抵抗性品種の開発につながると期待される.

Outline of Final Research Achievements

In order to elucidate the resistant mechanisms of soybean for Spodoptera litura, the physiological changes within the body of the larvae of S. litura fed on the soybean variety "Himeshirazu" were clarified, and the factors that cause these changes were investigated. Focusing on digestive enzyme activity in the midgut, we measured enzyme activity after accurately measuring pH in the midgut, and found a decrease in protease activity. Therefore, we examined the appropriate conditions for extracting protease inhibitors from the leaves of Himeshirazu. Then, we proceeded with purification using protease inhibitory activity as an indicator, and found a component characteristic of Himeshirazu.

Academic Significance and Societal Importance of the Research Achievements

これまでの、ダイズの抵抗性遺伝子の特定が重視され抵抗性の作用機構に関する研究はあまり進んでいない。本研究ではハスモンヨトウ幼虫の生理的な変化に着目し、抵抗性の作用機構の解明を目指した。本研究成果により、ダイズ品種「ヒメシラズ」を摂食したハスモンヨトウ幼虫において中腸内のプロテアーゼ活性が低下することが明らかになり、その原因物質(阻害剤)がヒメシラズに含まれることが明らかになった。この成果は、抵抗性の作用機構の理解に基づいた有用な強抵抗性品種の開発に繋がると期待される。

Report

(3 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • Research Products

    (6 results)

All 2021 2020

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (2 results)

  • [Journal Article] Evaluation of antixenosis in soybean against <i>Spodoptera litura</i> by dual-choice assay aided by a statistical analysis model: Discovery of a novel antixenosis in Peking2021

    • Author(s)
      Yano M, Inoue T, Nakata R, Teraishi M, Yoshinaga N, Ono H, Okumoto Y, Mori N
    • Journal Title

      Journal of Pesticide Science

      Volume: 46 Issue: 2 Pages: 182-188

    • DOI

      10.1584/jpestics.D21-006

    • NAID

      130008043160

    • ISSN
      1348-589X, 1349-0923
    • Year and Date
      2021-05-20
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] New Insights Into Dietary Toxin Metabolism: Diversity in the Ability of the Natricine Snake Rhabdophis tigrinus to Convert Toad-Derived Bufadienolides2021

    • Author(s)
      Inoue Takato、Nakata Ryu、Savitzky Alan H.、Yoshinaga Naoko、Mori Akira、Mori Naoki
    • Journal Title

      Journal of Chemical Ecology

      Volume: 47 Issue: 10-11 Pages: 915-925

    • DOI

      10.1007/s10886-021-01287-6

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Variation in Bufadienolide Composition of Parotoid Gland Secretion From Three Taxa of Japanese Toads2020

    • Author(s)
      Inoue Takato、Nakata Ryu、Savitzky Alan H.、Yoshinaga Naoko、Mori Akira、Mori Naoki
    • Journal Title

      Journal of Chemical Ecology

      Volume: 46 Issue: 10 Pages: 997-1009

    • DOI

      10.1007/s10886-020-01217-y

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Molecular basis underlying common cutworm resistance of the primitive soybean landrace Peking2020

    • Author(s)
      Nakata R, Yano M, Hiraga S, Teraishi M, Okumoto Y, Mori N, Kaga A
    • Journal Title

      Frontier in Genetics

      Volume: 11 Pages: 581917-581917

    • DOI

      10.3389/fgene.2020.581917

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ダイズの在来種 Peking が持つハスモンヨトウ抵抗性の分子基盤2021

    • Author(s)
      中田 隆,矢野 まりこ,平賀 勧,寺石 政義,奥本 裕,森 直樹,加賀 秋人
    • Organizer
      日本農薬学会第 46 回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 日本産ヒキガエルが有する耳腺毒成分, bufadienolideの多様性2021

    • Author(s)
      井上 貴斗,中田 隆,サヴィツキイ アラン,吉永 直子,森 哲,森 直樹
    • Organizer
      日本農芸化学会2021年度大会
    • Related Report
      2020 Research-status Report

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Published: 2020-09-29   Modified: 2023-01-30  

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