| Project/Area Number |
20K23057
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0907:Oral science and related fields
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| Research Institution | National Center for Geriatrics and Gerontology (2021)
The University of Tokushima (2020) |
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Principal Investigator |
ラジュ レスミ 国立研究開発法人国立長寿医療研究センター, ジェロサイエンス研究センター, 研究員 (40876702)
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| Project Period (FY) |
2020-09-11 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | regeneration / tissue engineering / periodontal tissue / tooth regeneration / Periodontal tissue / Regenerative medicine / Tissue engineering |
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| Outline of Research at the Start |
Periodontal tissue is composed of cementum, periodontal ligament (PDL) and alveolar bone, given the complexity of this redundant structure, a single type of cell is not enough to regenerate such combined tissue. Here we are developing a complex cell sheet composed of PDL and osteogenic differentiated PDL cells. Furthermore, we will be analyzing the regenerative capacity of this cell sheet by ectopic and orthotopic transplantation in mice.
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| Outline of Annual Research Achievements |
Complex cell sheet, CCS was made using rat PDL cells and osteogenic differentiated PDL cells. Transplants from ectopic transplantation analysis using histology and micro-CT showed the formation of periodontal ligament like bone like tissue. Furthermore, we have successfully created an orthotropic injury mice model for the transplantation of cell sheets. In a continuation study, we have evaluated the regenerative capacity of tooth dentin-pulp tissue by a CCS. Dental pulp stem cells, DPSCs and odontogenic differentiated DPSCs were utilized to fabricate a CCS. Ectopic transplants after 8 weeks of transplantation revealed the formation of dentin-pulp like combined hard and soft tissue formation.
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