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Genetic modificatoin of host crops to endow novel resistant traits against root parasitic weeds based on advanced biochemical knowledge

Research Project

Project/Area Number 20KK0131
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 38:Agricultural chemistry and related fields
Research InstitutionOsaka Metropolitan University (2022-2023)
Osaka Prefecture University (2020-2021)

Principal Investigator

Okazawa Atsushi  大阪公立大学, 大学院農学研究科, 准教授 (10294042)

Co-Investigator(Kenkyū-buntansha) 杉本 幸裕  神戸大学, 農学研究科, 教授 (10243411)
若林 孝俊  大阪公立大学, 大学院農学研究科, 特任研究員 (20843858)
Project Period (FY) 2020-10-27 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2023: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2022: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2021: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2020: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywords根寄生雑草 / ハマウツボ科 / インベルターゼ / 糖代謝 / ストリゴラクトン / 寄生雑草 / ハマウツボ / インベルターゼインヒビター
Outline of Research at the Start

ストライガなどの根寄生雑草は,主要穀類や経済上重要な作物に寄生することで,世界の農業に甚大な被害を及ぼしている。持続可能な開発目標(SDGs)である貧困および飢餓の克服には,根寄生雑草による農業被害を最小限に抑制する方策が必須である。本国際共同研究では,スーダンの研究者と共に,新規根寄生雑草防除戦略を実証する。具体的には,宿主作物に根寄生雑草に糖を奪われない形質や,根寄生雑草の発芽を誘導しない形質を付与する。根寄生雑草の克服を実現可能なものとし,開発途上国の発展に貢献することを目指す。

Outline of Final Research Achievements

Orobanchaceae root parasitic weeds are causing significant damage to agriculture worldwide. We have been involved in research projects to contribute to overcoming the agricultural damage caused by root parasitic weeds in Sudan as a partner country. This study aimed to acquire knowledge for application in the development of hosts with resistance to root parasitic weeds, which can be easily introduced in Sudan. Focusing on the translocation of sugars from the host to the root parasitic weed, the invertase inhibitor gene comp33755, which is involved in the regulation of invertase, and is expected to play a central role in this process, was found in the Orobanche minor. Biochemical analysis of the gene revealed its function and its potential for application in conferring root parasitic weed resistance traits on the host.

Academic Significance and Societal Importance of the Research Achievements

本研究では、ヤセウツボのトランスクリプトームデータよりインベルターゼインヒビターをコードすると考えられる comp33755 に注目し、その機能を生化学的に解析した。その結果、comp33755 はアポプラストで細胞壁インベルターゼインヒビターとして機能することが示唆された。宿主から転流によって運ばれてきたスクロースは、根寄生雑草と宿主の細胞間(アポプラスト)で細胞壁インベルターゼによる加水分解を受け、生じる単糖が根寄生雑草に取り込まれると予想される。したがって、この過程に comp33755 を介入させることで根寄生雑草への糖の取り込みが阻害される可能性があり、今後、その活用を検討したい。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (24 results)

All 2024 2023 2022 2021 Other

All Int'l Joint Research (4 results) Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (15 results) (of which Int'l Joint Research: 6 results) Remarks (1 results)

  • [Int'l Joint Research] National Center for Research(スーダン)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] National Center for Research(スーダン)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] National Centre for Reserchati(スーダン)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] National Centre for Reserch(スーダン)

    • Related Report
      2020 Research-status Report
  • [Journal Article] The structure–activity relationship of aryloxyacetylthioureas for the inhibition of <i>Orobanche minor</i> radicle elongation2023

    • Author(s)
      Okazawa Atsushi、Noda Shizuki、Mimura Yusuke、Fujino Kotaro、Wakabayashi Takatoshi、Ohta Daisaku、Sugimoto Yukihiro、Sonoda Motohiro
    • Journal Title

      Journal of Pesticide Science

      Volume: 48 Issue: 4 Pages: 149-155

    • DOI

      10.1584/jpestics.D23-034

    • ISSN
      1348-589X, 1349-0923
    • Year and Date
      2023-11-20
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] オロバンキの発芽における糖質代謝動態の理解と防除への応用2023

    • Author(s)
      岡澤敦司
    • Journal Title

      アグリバイオ

      Volume: 7 Pages: 949-954

    • Related Report
      2023 Annual Research Report
  • [Journal Article] Synthesis of aryloxyacetylthiourea derivatives for the development of radicle elongation inhibitor of parasitic weeds2023

    • Author(s)
      Sonoda Motohiro、Mimura Yusuke、Noda Shizuki、Okazawa Atsushi
    • Journal Title

      Tetrahedron

      Volume: 135 Pages: 133333-133333

    • DOI

      10.1016/j.tet.2023.133333

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Involvement of α-galactosidase OmAGAL2 in planteose hydrolysis during seed germination of <i>Orobanche minor</i>2021

    • Author(s)
      Okazawa Atsushi、Baba Atsuya、Okano Hikaru、Tokunaga Tomoya、Nakaue Tsubasa、Ogawa Takumi、Shimma Shuichi、Sugimoto Yukihiro、Ohta Daisaku
    • Journal Title

      Journal of Experimental Botany

      Volume: 73 Issue: 7 Pages: 1992-2004

    • DOI

      10.1093/jxb/erab527

    • Related Report
      2022 Research-status Report 2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Control of the root parasitic plant Orobanche minor through chemical inhibition of metabolism in germination2024

    • Author(s)
      Atsushi Okazawa, Motohioro Sonoda, Daisaku Ohta, Yukihiro Sugimoto
    • Organizer
      International Molecular Biology (IPMB) Congress 2024
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Screening of α-galactosidase OmAGAL2 inhibitors and their effect on germination and sugar profiles in Orobanche minor2024

    • Author(s)
      Atsushi Okazawa, Masahiro Onitsuka, Takatoshi Wakabayashi, Takumi Ogawa, Yukihiro Sugimoto, Daisaku Ohta
    • Organizer
      17th World Congress on Parasitic Plants
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Sugar profiling of root parasitic weed Orobanche minor and its host Trifolium pratense2024

    • Author(s)
      Eri Saiga, Moe Matsumoto, Takumi Ogawa, Eiichiro Fukusaki, Daisaku Ohta, Atsushi Okazawa
    • Organizer
      17th World Congress on Parasitic Plants
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 寄生雑草ヤセウツボにおける炭素収奪代謝経路の解析2024

    • Author(s)
      雑賀恵梨、松本萌、小川拓水、福崎英一郎、太田大策、岡澤敦司
    • Organizer
      日本農芸化学会 2024 年度東京大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Effect of aromatic carbonyl thioureas on radicle elongation of a root parasitic plant Orobanche minor2023

    • Author(s)
      Atsushi Okazawa, Shizuki Noda, Yusuke Mimura, Kotaro Fujino, Takatoshi Wakabayashi, Daisaku Ohta, Yukihiro Sugimoto, Motohiro Sonoda
    • Organizer
      15th IUPAC International Congress of Crop Protection Chemistry (ICCPC 2023)
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] 根寄生雑草の幼根伸長阻害活性が期待される芳香族チオウレアの合成2023

    • Author(s)
      三村勇介、野田鎮希、岡澤敦司、園田素啓
    • Organizer
      日本農薬学会第48回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] ヤセウツボ幼根伸長を阻害する芳香族チオウレアの構造活性相関2023

    • Author(s)
      岡澤敦司、三村勇介、野田鎮希、藤野宏太郎、若林孝俊、杉本幸裕、太田大策、園田素啓
    • Organizer
      日本農薬学会第48回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] トマトにおいて 18-oxo-CLA を基質とする orobanchol 合成酵素の同定2022

    • Author(s)
      本間大翔、若林孝俊、塩谷七洋、磯部一樹、岡澤敦司、太田大策、滝川浩郷、水谷正治、杉本幸裕
    • Organizer
      植物化学調節学会第 57 回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] ヤセウツボの α-ガラクトシダーゼに対する阻害剤のスクリーニングと発芽への影響評価2022

    • Author(s)
      鬼束将弘、若林孝俊、小川拓水、太田大策、杉本幸裕、岡澤敦司
    • Organizer
      植物化学調節学会第 57 回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] ヤセウツボ幼根伸長阻害効果を示す芳香族カルボニルチオウレアの構造活性相関2022

    • Author(s)
      岡澤敦司、三村勇介、野田鎮希、太田大策、杉本幸裕、園田素啓
    • Organizer
      植物化学調節学会第 57 回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Structure-activity relationship of aromatic carbonylthioureas for inhibition of Orobanche minor radicle elongation2022

    • Author(s)
      Atsushi Okazawa, Shizuki Noda, Yusuke Mimura, Daisaku Ohta, Yukihiro Sugimoto, Motohiro Sonoda
    • Organizer
      16th World Congress on Parasitic Plants
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Actinomycete metabolites for control of Orobanchaceae root parasitic weeds2021

    • Author(s)
      Atsushi Okazawa, Takatoshi Wakabayashi, Hiroaki Samejima, Kazuo Harada, Shigeru Kitani, Yukihiro Sugimoto, Daisaku Ohta
    • Organizer
      Pacifichem2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヤセウツボ発芽種子におけるプランテオース代謝の局在2021

    • Author(s)
      岡澤敦司、岡野ひかる、小川拓水、新間秀一、杉本幸裕、太田大策
    • Organizer
      第38回日本植物バイオテクノロジー学会(つくば)大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 根寄生雑草ヤセウツボの発芽におけるプランテオースの代謝部位2021

    • Author(s)
      岡澤敦司、岡野ひかる、新間秀一、小川拓水、杉本幸裕、太田大策
    • Organizer
      日本農薬学会第 46 回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] ヤセウツボ種子内でプランテオースを加水分解する α-ガラクトシダーゼの局在の解明2021

    • Author(s)
      岡野ひかる、小川拓水、杉本幸裕、太田大策、岡澤敦司
    • Organizer
      日本農芸化学会 2021 年度大会
    • Related Report
      2020 Research-status Report
  • [Remarks] プレスリリース;根寄生雑草防除の標的となる代謝酵素を特定―アフリカの飢餓克服への貢献をめざして―

    • URL

      https://www.osakafu-u.ac.jp/press-release/pr20211220/

    • Related Report
      2021 Research-status Report

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Published: 2020-10-29   Modified: 2025-01-30  

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