感覚モダリティ理解のためのミクログリア・シナプス接触の多角的解析

• Project/Area Number: 20KK0170
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 46:Neuroscience and related fields
• Research Institution: National Institute for Physiological Sciences (2021-2023); Nagoya University (2020)
• Principal Investigator: 和氣 弘明 生理学研究所, 基盤神経科学研究領域, 教授 (90455220)
• Co-Investigator(Kenkyū-buntansha): 大野 伸彦 自治医科大学, 医学部, 教授 (10432155) ; 加藤 大輔 生理学研究所, 基盤神経科学研究領域, 特別訪問研究員 (10712292) ; 竹田 育子 生理学研究所, 基盤神経科学研究領域, 特別訪問研究員 (30746300)
• Project Period (FY): 2020-10-27 – 2024-03-31
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2023: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2022: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2021: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
• Keywords: ミクログリア / トランスクリプトーム / 異種感覚可塑性 / シナプス / 異種感覚の可塑性 / 生体イメージング

Outline of Research at the Start

本国際研究では中枢神経系唯一の免疫細胞であるミクログリアに着目し、ミクログリアがシナプスの構造的・機能的可塑性を修飾する背景を踏まえ、多角的階層的な技術を相互補完することによって、ミクログリアのシナプスに対する時間的(発達・成熟)および空間(脳領域)特異的な生理機能を明らかにする。さらにこれを異種感覚の可塑的変化のメカニズムに繋げ、そこから精神病態を考察する。

Outline of Annual Research Achievements

ミクログリアによる異種感覚の可塑性への寄与を明らかにし、その精神疾患病態への関わりについて検証した。
まず、高次視覚野であるV2Lにそれぞれの第一次感覚野から投射があることを示し、V2Lにおける神経細胞の機能応答を検証したところ、通常ではヒゲ刺激によってV2Lの応答は抑制されるが、視覚遮断によってその活動頻度が増強することがわかった。詳細な検討を行ったところ、ミクログリアは視覚遮断に伴い、興奮性神経細胞周囲に接触し、抑制性シナプスを剥がすことによってその興奮性神経細胞の興奮性を獲得することに寄与することがわかった。さらにこの獲得した興奮性はヒゲの感覚識別に寄与することも明らかにした。これは先天的視覚遮断によって起こる現象である。では後天的視覚遮断ではどうなるのであろうか。ヒトでは極度の低視力もしくは視覚喪失に伴って幻覚が生じることが知られている（シャルルボネ症候群）。マウスでこれを検証するために成熟マウスで視覚遮断を行い、神経細胞の活動を検証したところ、神経細胞集団の活動の増加、ミクログリアの活性化それに伴うアストロサイトの活動増強が観察された。これらの神経活動に対する寄与を現在検証するとともに海外共同研究者とともに、トランスクリプトーム変化をそれぞれの細胞のRNAシークエンスを用いて解析している。さらに病態モデルにおける変化を海外共同研究者とともに、トランスクリプトーム変化とイメージング解析を統合し、検証している。さらにこれを病態に応用するために統合失調症および自閉症のリスクファクターとして知られる3q29欠失症候群に着目した。3q29欠失モデルマウスのミクログリアの形態変化を検証したところ3q29欠失モデルマウスではミクログリアの細胞体面積及び分岐数は前頭前野、連合野、第一次体性感覚野、第一次視覚野で低下していることがわかった。

Research Products

• [Int'l Joint Research] Icahn School of Medicine Mount Sinai(米国)
• [Int'l Joint Research] マウントサイナイ大学(米国)
• [Int'l Joint Research] UNSW(オーストラリア)
• [Int'l Joint Research] マウントサイナイ大学(米国)
• [Journal Article] Editorial: Oligodendrocytes: from their development to function and dysfunction (2024)
  • Author(s): Miyata S, Wake H
  • Journal Title: Frontiers in Cellular Neuroscience Volume: 18 Pages: 1376931-1376931
  • DOI: 10.3389/fncel.2024.1376931
  • Peer Reviewed / Open Access
• [Journal Article] Astrocytic NKCC1 inhibits seizures by buffering Cl- and antagonizing neuronal NKCC1 at GABAergic synapses (2023)
  • Author(s): Nguyen Trong Dao、Ishibashi Masaru、Sinha Adya Saran、Watanabe Miho、Kato Daisuke、Horiuchi Hiroshi、Wake Hiroaki、Fukuda Atsuo
  • Journal Title: Epilepsia Volume: 64 Issue: 12 Pages: 3389-3403
  • DOI: 10.1111/epi.17784
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Activity-dependent oligodendrocyte calcium dynamics and their changes in Alzheimer's disease (2023)
  • Author(s): Kenji Yoshida, Daisuke Kato, Shouta Sugio, Ikuko Takeda, Hiroaki Wake.
  • Journal Title: Front Cell Neurosci Volume: 17
  • DOI: 10.3389/fncel.2023.1154196
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of lipid synthesis in myelin modulates neural activity and is required for motor learning (2023)
  • Author(s): Kato D, Aoyama Y, Nishida K, Takahashi Y, Sakamoto T, Takeda I, Tatematsu T, Go S, Saito Y, Kunishima S, Cheng J, Hou L, Tachibana Y, Sugio S, Kondo R, Eto F, Sato S, Moorhouse AJ, Yao I, Kadomatsu K, Setou M, and Wake H.
  • Journal Title: Glia Volume: 71 Issue: 11 Pages: 2591-2608
  • DOI: 10.1002/glia.24441
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] KCC2 downregulation after sciatic nerve injury enhances motor function recovery (2023)
  • Author(s): Cheung Dennis Lawrence、Toda Takuya、Narushima Madoka、Eto Kei、Takayama Chitoshi、Ooba Tatsuko、Wake Hiroaki、Moorhouse Andrew John、Nabekura Junichi
  • Journal Title: Scientific Reports Volume: 13 Issue: 1
  • DOI: 10.1038/s41598-023-34701-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Microglia Enable Cross-Modal Plasticity by Removing Inhibitory Synapses (2023)
  • Author(s): Hashimoto A, Kawamura N, Tarusawa E, Takeda I, Aoyama Y, Ohno N, Inoue M, Kagamiuchi M, Kato D, Matsumoto M, Hasegawa Y, Nabekura J, Schaefer A, Moorhouse AJ, Yagi T, Wake H
  • Journal Title: Cell Reports Volume: - Issue: 5 Pages: 112383-112383
  • DOI: 10.1016/j.celrep.2023.112383
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CD206+ macrophages transventricularly infiltrate the early embryonic cerebral wall to differentiate into microglia. (2023)
  • Author(s): Hattori Y, Kato D, Murayama F, Koike S, Asai H, Yamasaki A, Naito Y, Kawaguchi A, Hiroyuki Konishi H, Prinz M, Masuda T, Wake H, Miyata T.
  • Journal Title: Cell Rep Volume: 42 Issue: 2 Pages: 112092-112092
  • DOI: 10.1016/j.celrep.2023.112092
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Myelinated axon as a plastic cable regulating brain functions. (2023)
  • Author(s): Sugio S, Kato D, Wake H.
  • Journal Title: Neurosci Res. Volume: 187 Pages: 45-51
  • DOI: 10.1016/j.neures.2022.11.002
  • Peer Reviewed / Open Access
• [Journal Article] Elucidation of the neurological effects of clothianidin exposure at the no-observed-adverse-effect level (NOAEL) using two-photon microscopy <i>in vivo</i> imaging (2022)
  • Author(s): NISHI Misaki、SUGIO Shouta、HIRANO Tetsushi、KATO Daisuke、WAKE Hiroaki、SHODA Asuka、MURATA Midori、IKENAKA Yoshinori、TABUCHI Yoshiaki、MANTANI Youhei、YOKOYAMA Toshifumi、HOSHI Nobuhiko
  • Journal Title: Journal of Veterinary Medical Science Volume: 84 Issue: 4 Pages: 585-592
  • DOI: 10.1292/jvms.22-0013
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lighting up cosmic neuronal networks with transformative in vivo calcium imaging (2022)
  • Author(s): Murayama Masanori、Wake Hiroaki
  • Journal Title: Neuroscience Research Volume: 179 Pages: 1-2
  • DOI: 10.1016/j.neures.2022.04.007
  • Peer Reviewed / Open Access
• [Journal Article] A Piezo1/KLF15/IL-6 axis mediates immobilization-induced muscle atrophy (2022)
  • Author(s): Yu Hirata, Kazuhiro Nomura, Daisuke Kato, Yoshihisa Tachibana, Takahiro Niikura, Kana Uchiyama, Tetsuya Hosooka, Tomoaki Fukui, Keisuke Oe, Ryosuke Kuroda, Yuji Hara, Takahiro Adachi, Koji Shibasaki, Hiroaki Wake, Wataru Ogawa
  • Journal Title: Journal of Clinical Investigation Volume: － Issue: 10 Pages: 1-13
  • DOI: 10.1172/jci154611
  • Peer Reviewed / Open Access
• [Journal Article] Controlled activation of cortical astrocytes modulates neuropathic pain-like behaviour (2022)
  • Author(s): Takeda Ikuko、Yoshihara Kohei、Cheung Dennis L.、Kobayashi Tomoko、Agetsuma Masakazu、Tsuda Makoto、Eto Kei、Koizumi Schuichi、Wake Hiroaki、Moorhouse Andrew J.、Nabekura Junichi
  • Journal Title: Nature Communications Volume: 13 Issue: 1 Pages: 4100-4100
  • DOI: 10.1038/s41467-022-31773-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evaluation and Manipulation of Neural Activity using Two-Photon Holographic Microscopy (2022)
  • Author(s): Kato Daisuke、Quan Xiangyu、Tanisumi Yuta、Guo Zhongtian、Morita Mitsuhiro、Takiguchi Tetsuya、Matoba Osamu、Wake Hiroaki
  • Journal Title: Journal of Visualized Experiments Volume: 187 Issue: 187 Pages: 1-15
  • DOI: 10.3791/64205
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ca2+ imaging with two-photon microscopy to detect the disruption of brain function in mice administered neonicotinoid insecticides (2022)
  • Author(s): Hirai Anri、Sugio Shouta、Nimako Collins、Nakayama Shouta M. M.、Kato Keisuke、Takahashi Keisuke、Arizono Koji、Hirano Tetsushi、Hoshi Nobuhiko、Fujioka Kazutoshi、Taira Kumiko、Ishizuka Mayumi、Wake Hiroaki、Ikenaka Yoshinori
  • Journal Title: Scientific Reports Volume: 12 Issue: 1 Pages: 5114-5114
  • DOI: 10.1038/s41598-022-09038-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 総説 ミクログリアによる血液脳関門機能の制御 (2021)
  • Author(s): 池上 暁湖、和氣 弘明
  • Journal Title: BRAIN and NERVE Volume: 73 Issue: 8 Pages: 913-919
  • DOI: 10.11477/mf.1416201861
  • ISSN: 1344-8129, 1881-6096
  • Year and Date: 2021-08-01
  • Peer Reviewed / Open Access
• [Journal Article] 特集 グリアと神経-相補的な制御系として 精神疾患の新たな展望-グリア破綻から見る病態 (2021)
  • Author(s): 有岡 祐子、加藤 大輔、和氣 弘明、尾崎 紀夫
  • Journal Title: BRAIN and NERVE Volume: 73 Issue: 7 Pages: 787-794
  • DOI: 10.11477/mf.1416201836
  • ISSN: 1344-8129, 1881-6096
  • Year and Date: 2021-07-01
  • Peer Reviewed
• [Journal Article] Holographic microscope and its biological application. (2021)
  • Author(s): Quan X, Kato D, Daria V, Matoba O, Wake H.
  • Journal Title: Neurosci Res Volume: - Pages: 1-1
  • DOI: 10.1016/j.neures.2021.10.012
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Pain induces stable, active microcircuits in the somatosensory cortex that provide a new therapeutic target. (2021)
  • Author(s): Okada T, Kato D, Nomura Y, Obata N, Quan X, Morinaga A, Yano H, Guo Z, Aoyama Y, Tachibana Y, Moorhouse AJ, Matoba O, Takiguchi T, Mizobuchi S and Wake H
  • Journal Title: Sci Adv Volume: 7 Issue: 12 Pages: 8261-8261
  • DOI: 10.1126/sciadv.abd8261
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Myelin plasticity modulates neural circuitry required for learning and behavior. (2021)
  • Author(s): Kato D, Wake H
  • Journal Title: Neurosci Res. Volume: - Pages: 11-16
  • DOI: 10.1016/j.neures.2020.12.005
  • NAID: 120007185093
  • Peer Reviewed / Open Access
• [Journal Article] Maternal Immune activation induces sustained changes in fetal microglia motility (2020)
  • Author(s): Ozaki K, Kato D, Ikegami A, Hashimoto A, Sugio S, Guo Z, Shibushita M, Tatematsu T, Haruwaka K, Moorhouse AJ, Yamada H, Wake H
  • Journal Title: Sci Rep Volume: 10 Issue: 1 Pages: 21378-21378
  • DOI: 10.1038/s41598-020-78294-2
  • NAID: 120006954848
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Negative feedback control of neuronal activity by microglia. (2020)
  • Author(s): Badimon A, Strasburger HJ, Ayata P, Chen X, Nair A, Ikegami A, Hwang P, Chan AT, Graves SM, Uweru JO, Ledderose C, Kutlu MG, Wheeler MA, Kahan A, Ishikawa M, Wang YC, Loh YE, Jiang JX, Surmeier DJ, Robson SC, Junger WG, Sebra R, Calipari ES, Kenny PJ, Eyo UB, Colonna M, Quintana FJ, Wake H, Gradinaru V, Schaefer A.
  • Journal Title: Nature Volume: 586 Issue: 7829 Pages: 417-423
  • DOI: 10.1038/s41586-020-2777-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transient microglial absence assists postmigratory cortical neurons in proper differentiation (2020)
  • Author(s): Yuki Hattori, Yu Naito, Yoji Tsugawa, Shigenori Nonaka, Hiroaki Wake, Takashi Nagasawa, Takaki Miyata
  • Journal Title: Nature Communications Volume: 11 Issue: 1 Pages: 1631-1631
  • DOI: 10.1038/s41467-020-15409-3
  • NAID: 120006841707
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] YAP1 mediates survival of ALK-rearranged lung cancer cells treated with alectinib via pro-apoptotic protein regulation (2020)
  • Author(s): Tsuji Takahiro、Ozasa Hiroaki、Aoki Wataru、Aburaya Shunsuke、Yamamoto Funazo Tomoko、Furugaki Koh、Yoshimura Yasushi、Yamazoe Masatoshi、Ajimizu Hitomi、Yasuda Yuto、Nomizo Takashi、Yoshida Hironori、Sakamori Yuichi、Wake Hiroaki、Ueda Mitsuyoshi、Kim Young Hak、Hirai Toyohiro
  • Journal Title: Nature Communications Volume: 11 Issue: 1 Pages: 19-19
  • DOI: 10.1038/s41467-019-13771-5
  • NAID: 120006782216
  • Peer Reviewed / Open Access
• [Presentation] Alteration in the Blood-Brain Barrier and microglia in a mouse model of Alzheimer’s disease. (2024)
  • Author(s): Lingnan Hou, Jinlei Cheng, Ikuko Takeda, Zhongtian Guo, Wanying Li, Daisuke Kato, Hiroaki Wake
  • Organizer: 第101回 日本生理学会大会
• [Presentation] 多感覚統合をつかさどる高次視覚野の神経回路基盤 (2024)
  • Author(s): Mio Inoue, Yuta Taisumi, Ikuko Takeda, Akari Hashimoto, Nanami Kawamura, Etsuko Tarusawa, Daisuke Kato, Hiroaki Wake
  • Organizer: 第129回日本解剖学会
• [Presentation] Microglia Mediate Synaptic Loss in the Early Stages of Alzheimer's Disease (2024)
  • Author(s): Zhongtian Guo, Daisuke Kato, Reon Kondo, Ikuko Takeda, Hiroaki Wake
  • Organizer: 第101回 日本生理学会大会
• [Presentation] Microglia enable cross-modal plasticity by removing inhibitory synapses (2023)
  • Author(s): Akari HASHIMOTO, Nanami KAWAMURA, Etsuko TARUSAWA, Ikuko TAKEDA, Yuki AOYAMA, Nobuhiko OHNO, Mio INOUE, Mai KAGAMIUCHI, Daisuke KATO, Mami MATSUMOTO, Yoshihiro HASEGAWA, Anne SCHAEFER, Junichi NABEKURA, Takeshi YAGI, Hiroaki WAKE
  • Organizer: 50th Naito Conference
  • Int'l Joint Research
• [Presentation] Cross-modal plasticity after early visual deprivation depends on microglial elimination of inhibitory synapses (2023)
  • Author(s): Akari HASHIMOTO, Nanami KAWAMURA, Etsuko TARUSAWA, Ikuko TAKEDA, Yuki AOYAMA, Nobuhiko OHNO, Mio INOUE, Mai KAGAMIUCHI, Daisuke KATO, Mami MATSUMOTO, Yoshihiro HASEGAWA, Anne SCHAEFER, Junichi NABEKURA, Takeshi YAGI, Hiroaki WAKE
  • Organizer: Neuroscience2023
• [Presentation] Holographic microscope illuminates brain activity (2023)
  • Author(s): Daisuke Kato, Xiangyu Quan, Osamu Matoba, Hiroaki Wake
  • Organizer: BISC2023
  • Int'l Joint Research
• [Presentation] Microglia in multi-sensory modality (2023)
  • Author(s): Hiroaki Wake, Akari Hashimoto
  • Organizer: Cold Spring Harbor Asia(Novel Insight into Glia Function & Dysfunction,AWAJI,JAPAN)
  • Int'l Joint Research / Invited
• [Presentation] Microglia in multi-sensory modality (2023)
  • Author(s): Hiroaki WAKE
  • Organizer: 50th Naito Conference
  • Int'l Joint Research / Invited
• [Presentation] Physiological Functions of Glial Cell for Brain Functions (2023)
  • Author(s): Hiroaki WAKE
  • Organizer: The 10th Federation of the Asian and Oceanian Physiological Societies Congress
  • Int'l Joint Research / Invited
• [Presentation] Astrocyte-mediated neuronal circuit remodeling in higher visual cortex induced by sensory deprivation (2023)
  • Author(s): Ikuko Takeda, Izumo Takei, Yuki Aoyama, Hiroaki Wake
  • Organizer: CSH Asia, Novel Insights into Glia Function & Dysfunction
  • Int'l Joint Research
• [Presentation] The role of astrocytes in V2L neuronal remodeling following vision loss (2023)
  • Author(s): Ikuko Takeda, Izumo Takei, Mio Inoue,Mai Kagamiuchi,Akari Hashimoto,Yuki Aoyama, Hiroaki Wake
  • Organizer: ISN-ESN meeting
  • Int'l Joint Research
• [Presentation] The role of astrocytes following vision loss (2023)
  • Author(s): Ikuko Takeda, Akari Hashimoto, Izumo Takei, Mio Inoue, Mai Kagamiuchi, Daisuke Kato, Hiroaki Wake
  • Organizer: 50th Naito Conference
  • Int'l Joint Research
• [Presentation] The role of astrocytes in V2L neuronal remodeling following vision loss (2023)
  • Author(s): Ikuko Takeda, Akari Hashimoto, Izumo Takei, Mio Inoue, Hiroaki Wake
  • Organizer: Neuroscience2023
  • Int'l Joint Research
• [Presentation] The function of microglia on multi-sensory integration in the V2L (2023)
  • Author(s): Mai Kagamiuchi, Akari Hashimoto, Mariko Shindo, Ikuko Takeda, Hiroaki Wake
  • Organizer: 第46回日本神経科学学会
• [Presentation] Neuronal circuit for multisensory integration in higher visual cortex (2023)
  • Author(s): Mio Inoue, Yuta Taisumi, Ikuko Takeda, Akari Hashimoto, Nanami Kawamura, Etsuko Tarusawa, Daisuke Kato, Hiroaki Wake
  • Organizer: 第46回日本神経科学学会
• [Presentation] Neuronal circuit for multisensory integration in higher visual cortex (2023)
  • Author(s): Mio Inoue, Yuta Taisumi, Ikuko Takeda, Akari Hashimoto, Nanami Kawamura, Etsuko Tarusawa, Daisuke Kato, Hiroaki Wake
  • Organizer: Neuroscience2023
  • Int'l Joint Research
• [Presentation] Microglial immune response for brain metastasis associating with annexin A13 (2023)
  • Author(s): Misuzu Horikoshi, Takahiro Tsuji, Mariko Shindo, Rahadian Yudo Hartantio, Daisuke Kato, Hiroaki Wake
  • Organizer: 第46回日本神経科学学会
• [Presentation] アネキシンA13が関与する転移性脳腫瘍へのミクログリア免疫応答 (2023)
  • Author(s): 堀越 水涼、辻 貴宏、進藤 麻理子、Rahadian Y. Hartantyo、加藤 大輔、和氣 弘明,
  • Organizer: 第82回日本癌学会学術総会
• [Presentation] Contribution of oligodendrocytes impairment to cognitive decline with aging (2023)
  • Author(s): Shiho Kunishima, Daisuke Kato, Jinglei Cheng, Hiroaki Wake
  • Organizer: Neuroscience2023
  • Int'l Joint Research
• [Presentation] Myelin lipid synthesis modulates neural activity and is essential for motor learning (2023)
  • Author(s): Y. AOYAMA, D. KATO, K. NISHIDA, Y. TAKAHASHI, T. SAKAMOTO, I. TAKEDA, T. TATEMATSU, S. SUGIO, Y. SAITO, S. KUNISHIMA, J. CHENG, H. LINGNAN, Y. TACHIBANA, S. SUGIO, R. KONDO, F. ETO, S. SATO, A. MOORHOUSE, I. YAO, K. KADOMATSU, M. SETOU, H. WAKE
  • Organizer: Neuroscience2023
  • Int'l Joint Research
• [Presentation] Fusion experimental system of mouse sensory learning and multi-photon holographic microscopy (2023)
  • Author(s): Yuta Tanisumi, Syuto Hayashi, Xiangyu Quan, Daisuke Kato, Hiroaki Wake
  • Organizer: 第46回日本神経科学学会
• [Presentation] Interdisciplinary method to elucidate the interaction between brain microenvironment and cancer cells (2023)
  • Author(s): Takahiro Tsuji; Hiroaki Wake; Mariko Shindo; Rahadian Y. Hartantio; Misuzu Horikoshi; Daisuke Kato; Hiroaki Ozasa; Toyohiro Hirai
  • Organizer: AACR Annual meeting 2023 in Orlando
  • Int'l Joint Research
• [Presentation] Heterogeneous microglial response to cancer cells visualized by in vivo imaging model (2023)
  • Author(s): Takahiro TSUJI, Mariko SHINDO, Daisuke KATO, Hiroaki WAKE
  • Organizer: CSH Asia, Novel Insights into Glia Function & Dysfunction
  • Int'l Joint Research
• [Presentation] Heterogeneous microglial response to cancer cells visualized by in vivo imaging model (2023)
  • Author(s): Takahiro Tsuji; Mariko Shindo; Rahadian Y. Hartantio; MisuzuHorikoshi; Daisuke Kato; Hiroaki Wake
  • Organizer: ISN-ESN meeting
  • Int'l Joint Research
• [Presentation] 多細胞回路動態の計測と操作を目指して (2023)
  • Author(s): 和氣弘明
  • Organizer: 第127回日本眼科学会
• [Presentation] オリゴデンドロサイトの神経回路同期性への寄与 (2023)
  • Author(s): 和氣 弘明
  • Organizer: 第46回日本神経組織培養研究会
• [Presentation] 中枢神経系多細胞回路の生理機能およびその計測と操作 (2023)
  • Author(s): 和氣 弘明
  • Organizer: 第8回日本骨免疫学会ウインタースクール
• [Presentation] オリゴデンドロサイトのよる神経回路の同期性制御 (2023)
  • Author(s): 和氣弘明
  • Organizer: 第100回日本生理学会大会
• [Presentation] Astrocyte-mediated neuronal circuit remodeling in higher visual cortex induced by sensory deprivation (2023)
  • Author(s): 竹田 育子、竹井 いずも、青山 友紀、和氣 弘明
  • Organizer: 日本生理学会第100回記念大会
• [Presentation] Role of microglia in higher visual sensory integration (2023)
  • Author(s): 鏡内 麻以、竹田 育子、和氣 弘明
  • Organizer: 日本生理学会第100回記念大会
• [Presentation] Physiological function of the neuronal projection between different sensory modality (2023)
  • Author(s): 井上 澪、橋本 明香里、竹田 育子、加藤 大輔、和氣 弘明
  • Organizer: 日本生理学会第100回記念大会
• [Presentation] Holographic microscopy for biological applications (2023)
  • Author(s): Daisuke Kato, Xiangyu Quan, Osamu Matoba, Hiroaki Wake
  • Organizer: 日本生理学会第100回記念大会
• [Presentation] 生体イメージングで解き明かすグリア細胞の新規生理機能とその病態への寄与 (2022)
  • Author(s): 和氣弘明
  • Organizer: 第63回日本神経学会学術大会
  • Invited
• [Presentation] 多細胞回路動態の計測と操作 (2022)
  • Author(s): 和氣弘明
  • Organizer: Cardiovascular Research Conference
  • Invited
• [Presentation] 多細胞回路動態の計測と操作 (2022)
  • Author(s): 和氣弘明
  • Organizer: Parkinson's Disease Expert Meeting～新たな治療選択肢を考える～
  • Invited
• [Presentation] ミクログリアの新規生理機能 (2022)
  • Author(s): 和氣弘明
  • Organizer: 第４回量子生命科学会
  • Invited
• [Presentation] Microglia and sensory modality (2022)
  • Author(s): Hiroaki WAKE
  • Organizer: NEURO2022
• [Presentation] 光による神経・グリア生理活動の叙述と制御 (2022)
  • Author(s): 和氣弘明
  • Organizer: 第43回日本炎症・再生医学会（淡路島）
• [Presentation] グリア細胞の生理機能と病態への関与 (2022)
  • Author(s): 和氣弘明
  • Organizer: 第16回日本パーキンソン病・運動障害疾患コングレス
• [Presentation] Microglia and sensory modality (2022)
  • Author(s): Hiroaki WAKE
  • Organizer: Glia and Neuroimmune symposium held at Anhui Medical University
  • Int'l Joint Research
• [Presentation] Microglia and sensory modality (2022)
  • Author(s): Hiroaki WAKE
  • Organizer: ISN-APSN2022Biennial Meeting
  • Int'l Joint Research
• [Presentation] Physiological and pathological functions of microglia (2022)
  • Author(s): Hiroaki WAKE
  • Organizer: 第95回日本生化学会大会
• [Presentation] Microglial removal of inhibitory synapses unleash the multi-sensory potential in the association cortex (2022)
  • Author(s): Hiroaki Wake, Akari Hashimoto, Nanami Kawamura, Etsuko Tarusawa, Ikuko Takeda, Nobuhiko Ohno, Mio Inoue, Daisuke Kato, Yuki Aoyama, Mami Matsumoto, Yoshihiro Hasegawa
  • Organizer: The Australian Physiological Society annual meeting
• [Presentation] Toward measurement and manipulation of multicellular circuit dynamics (2022)
  • Author(s): Hiroaki WAKE
  • Organizer: 第45回分子生物学会
• [Presentation] Physiological and Pathological functions of microglia (2022)
  • Author(s): Hiroaki WAKE
  • Organizer: 第51 回日本免疫学会学術集会
• [Presentation] 光で階層的に神経・グリア回路活動を叙述し、制御する研究を目指して (2022)
  • Author(s): 和氣弘明
  • Organizer: レーザー学会年次大会
• [Presentation] Physiological function of the tactile-visual connection (2022)
  • Author(s): Mio Inoue, Akari Hashimoto, Ikuko Takeda, Daisuke Kato, Hiroaki Wake
  • Organizer: Neuro2022 (第45回神経科学大会）
• [Presentation] Neuronal-circuite based mechanisms of chromotherapy for chronic pain (2022)
  • Author(s): Sumire Kato,Ikuko Takeda, Hiroaki Wake
  • Organizer: Neuro2022 (第45回神経科学大会）
• [Presentation] The alteration of astrocytes in cross-modal plasticity (2022)
  • Author(s): Ikuko Takeda, Izumo Takei, Yuki Aoyama, Hiroaki Wake
  • Organizer: Neuro2022 (第45回神経科学大会）
• [Presentation] Oligodendrocytes promote motor learning through temporally regulated lipid production (2022)
  • Author(s): Yuki Aoyama, Daisuke Kato, Kazuki Nishida, Fumihiro Eto, Shumpei Sato, Ikuko Yao, Mitsutoshi Setou and Hiroaki Wake
  • Organizer: 4th-UK-Japan Neuroscience Symposium 2022, Karuizawa, Japan
• [Presentation] Microglial phagocytosis suppressed the development of metastatic tumors in the imaging model of brain metastasis (2022)
  • Author(s): Takahiro Tsuji, Hiroaki Wake, Mariko. Shindo, Daisuke Kato, Hiroaki Ozasa, Toyohiro Hirai
  • Organizer: AACR Annual Meeting 2022
  • Int'l Joint Research
• [Presentation] Microglial phagocytosis suppressed the development of metastatic tumors in the imaging model of brain metastasis (2022)
  • Author(s): 辻貴宏、和氣弘明、進藤麻理子、加藤大輔、小笹裕晃、平井豊博
  • Organizer: Neuro2022 (第45回神経科学大会）
• [Presentation] SLE-induced CNS inflammation causes anxiety-like behavior (2022)
  • Author(s): Mariko Shindo, Takahiro Tsuji, Rahadian Yudo Hartantyo, Ataka Ito, Daisuke Kato, Takayoshi Suganami, Hiroaki Wake
  • Organizer: Neuro2022 (第45回神経科学大会）
• [Presentation] 健康社会の創成と国際連携に向けた多次元脳・生体イメージングセンターの構築 (2022)
  • Author(s): 和氣弘明
  • Organizer: 第95回日本薬理学会年会
  • Invited
• [Presentation] ミクログリアがコードする情報の読み出しへの挑戦 (2022)
  • Author(s): 和氣弘明
  • Organizer: 第95回日本薬理学会年会
• [Presentation] 脳免疫細胞の生理機能とその破綻による中枢神経系疾患 (2021)
  • Author(s): 和氣弘明
  • Organizer: 第6回日本骨免疫学会（招待講演）
  • Invited
• [Presentation] 精神疾患と脳脊髄液・脳血液関門 (2021)
  • Author(s): 和氣弘明
  • Organizer: BPNP2021
  • Invited
• [Presentation] 精神疾患におけるグリア細胞 (2021)
  • Author(s): 和氣弘明
  • Organizer: 千里ライフサイエンスセミナー
• [Presentation] 光で照らすグリア細胞の新規生理機能とその病態への寄与 (2021)
  • Author(s): 和氣弘明
  • Organizer: 生体機能と創薬シンポジウム
  • Invited
• [Presentation] グリアの貪食能による脳の生理・病態制御 (2021)
  • Author(s): 和氣弘明
  • Organizer: 第64回日本神経化学大会
• [Presentation] Illuminate the physiological and pathological function of microglia (2021)
  • Author(s): Hiroaki Wake
  • Organizer: JSPS-NIH forum
  • Int'l Joint Research / Invited
• [Presentation] 脳免疫細胞ミクログリアの生理機能と病態への寄与 (2020)
  • Author(s): 和氣弘明
  • Organizer: 第41回日本炎症・再生医学会
  • Invited
• [Presentation] ミクログリアによる微小脳環境の創造的破壊 (2020)
  • Author(s): 和氣弘明
  • Organizer: 第43回日本神経科学大会
• [Presentation] 神経細胞の計測・操作一体型顕微鏡の開発 (2020)
  • Author(s): 和氣弘明
  • Organizer: 第43回日本神経科学大会
  • Invited
• [Presentation] グリア細胞の生体内での挙動について (2020)
  • Author(s): 和氣弘明
  • Organizer: 日本臨床視覚生理学会
  • Invited
• [Presentation] 解き明かされる環境依存的可変型ミクログリアの生理機能 (2020)
  • Author(s): 和氣弘明
  • Organizer: 第63回日本神経化学会大会