Cerebral spine synapses and exocytosis studied with two-photon microscope

• Project/Area Number: 21000009
• Research Category: Grant-in-Aid for Specially Promoted Research
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: KASAI Haruo 東京大学, 大学院医学系研究科, 教授 (60224375)
• Co-Investigator(Renkei-kenkyūsha): MATSUZAKI Masanori 基礎生物学研究所, 教授 (50353438) ; TAKAHASHI Noriko 東京大学, 大学院医学系研究科, 講師 (60332178) ; NOGICHI Jun 東京大学, 大学院医学系研究科, 助教 (40421367) ; WATANABE Satoshi 東京大学, 大学院医学系研究科, 助教 (80302610)
• Project Period (FY): 2009 – 2014
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥379,860,000 (Direct Cost: ¥292,200,000、Indirect Cost: ¥87,660,000); Fiscal Year 2013: ¥78,520,000 (Direct Cost: ¥60,400,000、Indirect Cost: ¥18,120,000); Fiscal Year 2012: ¥88,920,000 (Direct Cost: ¥68,400,000、Indirect Cost: ¥20,520,000); Fiscal Year 2011: ¥88,920,000 (Direct Cost: ¥68,400,000、Indirect Cost: ¥20,520,000); Fiscal Year 2010: ¥123,500,000 (Direct Cost: ¥95,000,000、Indirect Cost: ¥28,500,000)
• Keywords: シナプス / 学習記憶 / 顕微鏡 / グルタミン酸 / 分泌 / 樹状突起スパイン / ドーパミン / 強化学習 / ヘブ学習 / スパインシナプス / カルシウムシグナル / 開口放出 / 精神疾患 / 生物・生体工学 / シグナル伝達 / 生理学 / 糖尿病 / 脳・神経

Outline of Final Research Achievements

We had already found that spine synapses undergo structural plasticity by glutamate, but we have further revealed the actions of GABA and dopamine on the structural plasticity of dendritic spines, which likely underlies animal behaviors. We also established a new way to image static and dynamic states of SNARE proteins for exocytosis in neuron and secretory cells using 2pFRET/FLIM, which will pave the way for comprehensive understanding of neuronal memory circuits.

Assessment Rating

Verification Result (Rating)

A

Research Products

• [Journal Article] PAKs inhibitors ameliorate schizophrenia-associated dendritic spine deterioration in vitro and in vivo during late adolescence (2014)
  • Author(s): Hayashi-Takagi, A., Araki, Y., Nakamura, M., Vollrath, B., Duron, S.G., Yan, Z., Kasai, H., Huganir, R.L., Campbell, D.A. & Sawa, A.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 111 Issue: 17 Pages: 6461-6
  • DOI: 10.1073/pnas.1321109111
  • Peer Reviewed
• [Journal Article] Sub-diffraction resolution pump-probe microscopy with shot-noise limited sensitivity using laser diodes (2014)
  • Author(s): Miyazaki, J., Tsurui, H., Hayashi-Takagi, A., Kasai, H. & Kobayashi, T.
  • Journal Title: Opt Express Volume: 22 Issue: 8 Pages: 9024-32
  • DOI: 10.1364/oe.22.009024
  • Peer Reviewed / Open Access
• [Journal Article] Caged Glutamates with π-Extended 1, 2-Dihydronaphthalene Chromophore : Design, Synthesis, Two-Photon Absorption Property, and Photochemical Reactivity (2014)
  • Author(s): Boinapally, S., Huang, B., Abe, M., Katan, C., Noguchi, J., Watanabe, S., Kasai, H., Xue, B. & Kobayashi, T.
  • Journal Title: Opt Express Volume: 22 Issue: 17 Pages: 7822-30
  • DOI: 10.1021/jo501425p
  • Peer Reviewed
• [Journal Article] Lab-on-a-brain : Implantable micro-optical fluidic devices for neural cell analysis in vivo (2014)
  • Author(s): Takehara, H.*, Nagaoka, A.*, Noguchi, J., Akagi, T., Kasai, H. & Ichiki, T.
  • Journal Title: Scientific Report Volume: 4 Issue: 1 Pages: 6721-6721
  • DOI: 10.1038/srep06721
  • Peer Reviewed
• [Journal Article] PAK inhibitors rescue DISC1-deficit-triggered dendritic spine deterioration : novel therapeutic potential for schizophrenia. (2014)
  • Author(s): Hayashi-Takagi, A., Araki, Y., Nakamura, M., Vollrath, B., Duron, S. G., Yan, Z., Kasai, H., Huganir, R. L., Campbell, D. A., Sawa, A.
  • Journal Title: Proc. Natl. Acad. Sci. U. S. A. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Spatiolemporal dynamics of functional clusters of neurons in mouse motor corlex during a voluntary movement (2013)
  • Author(s): Hira R, Okubo F, Ozawa K, Isomura Y, KHamura K, Kano M, Kasai H, Matsuzaki M
  • Journal Title: Journal of Neuroscience Volume: 33 Issue: 4 Pages: 1377-90
  • DOI: 10.1523/jneurosci.2550-12.2013
  • Peer Reviewed
• [Journal Article] GABA promotes the competitive selection of dendritic spines by controlling local Ca2+ signaling. Nature Neurosci. 16:1409-1416. (2013)
  • Author(s): Hayama, T., Noguchi, J., Watanabe, S., Ellis-Davies, G. C. R., Hayashi, A., Takahashi, N., Matsuzaki, M. & Kasai, H.
  • Journal Title: Nature Neurosci. Volume: 16 Issue: 10 Pages: 1409-1416
  • DOI: 10.1038/nn.3496
  • Peer Reviewed
• [Journal Article] Evaluation of dialkylaminofluorene-based hemicyanine dyes for second harmonic generation imaging by the direct comparison approach. (2013)
  • Author(s): Nuriya, M, Momotake, A, Kimura, Y., Yasui, M., Kasai, H. & Arai, T.
  • Journal Title: Bull. Chemical Soc. Jpn. Volume: 86 Issue: 10 Pages: 1190-1192
  • DOI: 10.1246/bcsj.20130094
  • NAID: 10031201275
  • Peer Reviewed
• [Journal Article] Next-generation transgenic mice for optogenetic analysis of neural circuits. (2013)
  • Author(s): Asrican, B, Augustine, G. J., Berglund, K., Chen, S., Chow, N. Deisseroth, K., Feng, G., Gloss, B. Hira, R., Hoffman, C., Kasai, K., Katarya, M.
  • Journal Title: Frontier in Neural Circuits Volume: 7 Pages: 1-10
  • DOI: 10.3389/fncir.2013.00160
  • Peer Reviewed
• [Journal Article] Implementation of tetra-poly (ethylene glycol) hydrogel with high mechanical strength into microfluidic device technology. (2013)
  • Author(s): Takehara, H., Nagaoka, A., Noguchi, J., Akagi, T., Sakai, T., Chung, U., Kasai, H. & Ichiki, T.
  • Journal Title: Biomicrofluidics Volume: 7 Issue: 5 Pages: 1-9
  • DOI: 10.1063/1.4822033
  • Peer Reviewed
• [Journal Article] Polarity-dependent photophysical properties of hemicyanine dyes and their application in 2-photon microscopy biological imaging. (2012)
  • Author(s): Kimura, Y., Momotake, A., Takahashi, N., Kasai, H. & Arai, T.
  • Journal Title: Chemistry Letters Volume: 41 Issue: 5 Pages: 528-530
  • DOI: 10.1246/cl.2012.528
  • NAID: 10030619751
  • Peer Reviewed
• [Journal Article] A novel function of Noc2 in agonist-induced intracellular Ca2+ increase during zymogen-granule exocytosis in pancreatic acinar cells. (2012)
  • Author(s): Ogata, S., Miki, T., Seino, S., Tamai, S., Kasai, H. & Nemoto, T.
  • Journal Title: PLoS ONE Volume: e37048 Issue: 5 Pages: 1-9
  • DOI: 10.1371/journal.pone.0037048
  • Peer Reviewed
• [Journal Article] Distinct initial SNARE configurations underlying the diversity of exocytosis. (2012)
  • Author(s): Kasai, H., Takahashi, N. & Tokumaru, H.
  • Journal Title: Physiol. Rev. Volume: 92 Issue: 4 Pages: 1915-1964
  • DOI: 10.1152/physrev.00007.2012
  • Peer Reviewed
• [Journal Article] In vivo two-photon uncaging of glutamate revealing the structure-function relationships o f dendritic spines in the neocortex of adult mice. (2011)
  • Author(s): Noguchi, J., Nagaoka, A., Watanabe, S., Ellis-Davies, G.C.R., Kitamura, K., Kano, M., Matsuzaki, M. & Kasai H.
  • Journal Title: J.Physiol. Volume: 589 Issue: 10 Pages: 2320-2329
  • DOI: 10.1113/jphysiol.2011.207100
  • Peer Reviewed
• [Journal Article] Spatial distributions of GABA receptors and local inhibition of Ca^<2+> transients studied with GABA uncaging in the dendrites of CA1 pyramidal neurons (2011)
  • Author(s): Yuya Kanemoto, Masanori Matsuzaki, Susumu Morita, Tatsuya Hayama, Jun Noguchi, Naoko Senda, Atsuya Momotake, Tatsuo Arai, Haruo Kasai
  • Journal Title: PLoS One Volume: 6 Issue: 7 Pages: e22652-e22652
  • DOI: 10.1371/journal.pone.0022652
  • Peer Reviewed
• [Journal Article] Two-Photon Uncaging Microscopy. (2011)
  • Author(s): Matsuzaki, M. & Kasai, H.
  • Journal Title: Cold Spring Harbor protocols Volume: 2011 Issue: 5 Pages: 555-561
  • DOI: 10.1101/pdb.prot5620
• [Journal Article] Simultaneous visualization of multiple neuronal properties with single-cell resolution in the living rodent brain. (2011)
  • Author(s): Imaging in Neuroscience: A Laboratory Manual (eds.Helmchen and Konnerth). CSHL Press, Cold Spring Harbor, NY,USA.
  • Journal Title: Mol. Cell. Neurosci. Volume: 48 Issue: 3 Pages: 246-257
  • DOI: 10.1016/j.mcn.2011.08.005
  • Peer Reviewed
• [Journal Article] Simultaneous two-photon activation of presynaptic cells and calcium imaging in postsynaptic dendritic spines (2011)
  • Author(s): Matsuzaki, M., Ellis-Davies, G.C.R., Kanemoto, Y., Kasai, H.
  • Journal Title: Neural Systems and Circuits Volume: 1 Pages: 2-2
  • Peer Reviewed
• [Journal Article] Decreased in the number of dense core vesicles is responsible for the impaired secretion if insulin in IA-2 and IA-2ss null mice (2011)
  • Author(s): Cai, T., Hirai, H., Zhang, G, Zhang, M., Takahashi, N., Kasai, H., Satin, L.S., Leapman, R.D., Notkins, A.L.
  • Journal Title: Diabetologia Volume: 54 Pages: 2347-2357
  • Peer Reviewed
• [Journal Article] 1-Acyl-5-methoxy-8-nitro-1,2-dihydroquinoline : A biologically useful photolabile precursor of carboxylic acids (2010)
  • Author(s): Obi, N., Momotake, A., Kanemoto, Y., Matsuzaki, M, Kasai, H., Arai, T.
  • Journal Title: Tetrahedron letters Volume: 51 Pages: 1642-1657
  • Peer Reviewed
• [Journal Article] Learning rules and persistence of dendritic spines (2010)
  • Author(s): Kasai, H., Hayama, T., Ishikawa, M., Watanabe, S., Yagishita, S.
  • Journal Title: Eur.J.Neurosci. Volume: 32 Pages: 241-249
  • Peer Reviewed
• [Journal Article] SNARE conformational changes that prepare vesicles for exocytosis (2010)
  • Author(s): Takahashi, N., Hatakeyama, H., Okado, H., Noguchi, J.Ohno, M., Kasai, H.
  • Journal Title: Cell Metabolism Volume: 12 Pages: 19-29
  • Peer Reviewed
• [Journal Article] Class IA phosphoinositide 3-kinase in pancreatic β cells controls insulin secretion by multiple mechanisms (2010)
  • Author(s): Kaneko, K., Ueki, K., Takahashi, N., Hashimoto, S., Okamoto, M., Awazawa, M., Okazaki, Y., Ohsugi, M., Inabe, K., Umehafa T., Yoshida M., Kakei M., Kitamura, T., Luo, J., Kulkarni, R.N., Kahn, C.R., Kasai, H., Cantley, L.C., Kadowaki, T.
  • Journal Title: Cell Metabolism Volume: 12 Pages: 619-632
  • Peer Reviewed
• [Journal Article] 1-Acyl-5-methoxy-8-nitro-1,2-dihydroquinoline : A biologically useful photolabile precursor of carboxylic acids (2010)
  • Author(s): Obi, N., et al.
  • Journal Title: Tetrahedron letters 51 Pages: 1642-1657
  • Peer Reviewed
• [Journal Article] Exocytosis in islet beta-cells (2010)
  • Author(s): Kasai, H., et al.
  • Journal Title: Advances in Experimental Medicine and Biology 654 Pages: 303-336
  • Peer Reviewed
• [Journal Article] Structural dynamics of dendritic spines in memory and cognition (2010)
  • Author(s): Kasai, H., et al.
  • Journal Title: Trends in Neurosciences 33 Pages: 121-129
  • Peer Reviewed
• [Journal Article] Two-color, two-photon uncaging of glutamate and GABA (2010)
  • Author(s): Kantevari, S., et al.
  • Journal Title: Nature Methods 7 Pages: 123-125
  • Peer Reviewed
• [Journal Article] Two photon uncaging of gamma-aminobutyric acid probes on neurons in intact brain tissue (2010)
  • Author(s): Matsuzaki, M., et al.
  • Journal Title: Nature Chemical Biology 6 Pages: 255-257
  • Peer Reviewed
• [Journal Article] Generation, elimination and weight fluctuations of synapses in the cerebral cortex (2009)
  • Author(s): Morita, S., et al.
  • Journal Title: Communicative and Integrative Biology 2 Pages: 1-4
  • Peer Reviewed
• [Presentation] Imaging and optogenetics of dendritic spines (2015)
  • Author(s): H. Kasai
  • Organizer: BSI seminar series
  • Place of Presentation: 理化学研究所(埼玉県和光市)
  • Year and Date: 2015-02-12
  • Invited
• [Presentation] The critical time window for dopamine actions on the structural plasticity of dendritic spines in the nucleus of accumbens (2014)
  • Author(s): H. Kasai & S. Yagishita
  • Organizer: Symposium on“Emotional mechanisms”
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2014-09-11
  • Invited
• [Presentation] Labeling and erasure of dendritic spines and motor learning with novel synaptic optogenetic probes in vivo (2014)
  • Author(s): H. Kasai & A. Hayashi
  • Organizer: Janelia Conference for“Structure and function of synapses”
  • Place of Presentation: Janelia Farm Research Campus(Ashburn, VA., USA)
  • Year and Date: 2014-04-02
  • Invited
• [Presentation] 内分泌細胞と神経細胞におけるSNARE複合化の2光子FLIM解析. (2014)
  • Author(s): 高橋倫子、澤田和可子、渡邉恵、大野光代、河西春郎
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス(鹿児島市)
  • Year and Date: 2014-03-18
• [Presentation] 半導体レーザーを用いた超解像誘導放出顕微鏡の開発と生体イメージングへの応用. (2014)
  • Author(s): 宮崎 淳1,2, 鶴井 博理2,3, 林(高木)朗子4, 河西 春郎2,4, 小林 孝嘉1,2
  • Organizer: 第61回応用物理学会
  • Place of Presentation: 青山学院大学相模原キャンパス(相模原市)
  • Year and Date: 2014-03-18
• [Presentation] シナプス前終末における開口放出関連蛋白SNAP25の構造と機能. (2014)
  • Author(s): 澤田和可子、高橋倫子、渡邉恵、大野光代、河西春郎
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス(鹿児島市)
  • Year and Date: 2014-03-17
• [Presentation] ドーパミンによる側坐核スパイン形態可塑性の鋭い時間枠での調節とその分子機構 (2014)
  • Author(s): 柳下 祥、林(高木)朗子、渡邉恵、河西春郎
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス(鹿児島市)
  • Year and Date: 2014-03-16
• [Presentation] ベータ細胞と神経終末の分泌分子機構の可視化解析 (2013)
  • Author(s): 河西春郎
  • Organizer: 朝日生命成人病研究所分泌セミナー
  • Place of Presentation: ノボノルディスクファーマ(株)本社アピスカフェ(東京都千代田区)
  • Year and Date: 2013-10-05
  • Invited
• [Presentation] 革新的的な脳計測・解析技術、「こころの健康社会の創造に向けて」 (2013)
  • Author(s): H. Kasai
  • Organizer: 日本学術会議主催学術フォーラム
  • Place of Presentation: 東京大学鉄門記念講堂(東京都文京区)
  • Year and Date: 2013-09-07
  • Invited
• [Presentation] Imaging synaptic function and plasticity. (2013)
  • Author(s): H. Kasai
  • Organizer: MRC-JST-British Embassy Tokyo Workshop : The use of next generation optical microscopy for neuroscience disease challenges
  • Place of Presentation: 在日本英国大使館(東京都千代田区)
  • Year and Date: 2013-09-05
  • Invited
• [Presentation] Structural plasticity of spine synapses in vitro and in vivo, Current trends and future directions of synaptic plasticity research (2013)
  • Author(s): H. Kasai
  • Organizer: US/Japan joint workshop
  • Place of Presentation: Seattle, USA
  • Year and Date: 2013-07-19
  • Invited
• [Presentation] A narrow time window for dopaminergic enhancement of spine structural plasticity in nucleus accumbens (2013)
  • Author(s): S. Yagishita, A. Hayashi-Takagi, S. Watanabe & H. Kasai
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館(京都市左京区)
  • Year and Date: 2013-06-21
• [Presentation] Two-photon FRET/FLIM imaging of SNARE-dependent exocytosis. 42th Annual Meeting of Society for Neuroscience (2012)
  • Author(s): N. Takahashi, M. Ohno, S. Watanabe, W. Sawada & H. Kasai
  • Organizer: 42th Annual Meeting of Society for Neuroscience
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2012-10-15
• [Presentation] Chronic in vivo imaging of dendritic spines with a microfluidic device and open-dura surgery in the adult mouse cortex. (2012)
  • Author(s): A. Nagaoka, H. Takehara, J. Noguchi, T. Akagi, T. Ichiki & H. Kasai
  • Organizer: 42th Annual Meeting of Society for Neuroscience
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2012-10-14
• [Presentation] The molecular basis of local synaptic competition between dendritic spines. (2012)
  • Author(s): T. Hayama, J. Noguchi, S. Watanabe, N. Takahashi, A. Hayashi-Takagi, G. C. R. Ellis-Davies, M. Matsuzaki & H. Kasai
  • Organizer: 42th Annual Meeting of Society for Neuroscience
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2012-10-13
• [Presentation] Synaptic competition in the dendritic spines. (2012)
  • Author(s): Haruo Kasai, Tatsuya Hayama, Jun Noguchi, Satoshi Watanabe
  • Organizer: Jacques-Monod Symposium in "Imaging neuronal functions: from molecules to circuits,"
  • Place of Presentation: Roscoff, France
  • Year and Date: 2012-06-30
  • Invited
• [Presentation] ２光子蛍光寿命画像法による開口放出SNARE機構の研究 (2012)
  • Author(s): 河西春郎、高橋倫子、大野光代、澤田和可子
  • Organizer: 日本腎臓学会
  • Place of Presentation: パシフィコ横浜会議センター(横浜市)
  • Year and Date: 2012-06-01
  • Invited
• [Presentation] Structural plasticity of spine synapses studied with two-photon microscope. (2012)
  • Author(s): Haruo Kasai
  • Organizer: International Symposium on in vivo microscopy
  • Place of Presentation: Helsinki, Finland
  • Year and Date: 2012-05-24
  • Invited
• [Presentation] Statistical dynamics of synapses (2011)
  • Author(s): Haruo Kasai
  • Organizer: System Neurobiology Spring School 2011
  • Place of Presentation: コープイン京都(京都)(招待講演)
  • Year and Date: 2011-03-13
• [Presentation] GABA dependence of locally competitive learning rules of dendritic spines revealed by two-color uncaging of glutamate and GABA (2010)
  • Author(s): Tatsuya Hayama, Yuya Kanemoto, Masanori Matsuzaki, Graham C.R.Ellis-Davies, Haruo Kasai
  • Organizer: 25^<th> Annual Meeting of Socienty for Neuroscience
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2010-11-17
• [Presentation] Imaging of synapses (2010)
  • Author(s): Haruo Kasai
  • Organizer: BSI tutorial course 2010
  • Place of Presentation: 理研BSI(埼玉)(招待講演)
  • Year and Date: 2010-11-17
• [Presentation] Optical stimulation illuminates synapses in the mammalian brain (2010)
  • Author(s): Haruo Kasai
  • Organizer: 新学術領域「分子行動学」シンポジウム
  • Place of Presentation: 東京大学小柴ホール(東京)(招待講演)
  • Year and Date: 2010-11-10
• [Presentation] SNARE conformational changes that prepare vesicle for exocytosis (2010)
  • Author(s): Haruo Kasai
  • Organizer: Biophysical Meeting
  • Place of Presentation: 北京国際会議場(中国)(招待講演)
  • Year and Date: 2010-10-11
• [Presentation] 2光子顕微鏡の神経科学領域への応用 (2010)
  • Author(s): 河西春郎
  • Organizer: 京都大学大学院神経科学コース
  • Place of Presentation: 京都大学医学部(京都)(招待講演)
  • Year and Date: 2010-09-15
• [Presentation] GABA dependence of glutamate-mediated shrinkage and enlimination of dendritic spines revealed with two-color glutamate and GABA (2010)
  • Author(s): Tatsuya Hayama, Yuya Kanemoto, Masanori Matsuzaki, Graham C.R.Ellis-Davies, Haruo Kasai
  • Organizer: 日本神経科学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2010-09-04
• [Presentation] In vivo two-photon uncaging in the adult brain (2010)
  • Author(s): 河西春郎
  • Organizer: Janelia Conference for structural plasticity in mammalian brain
  • Place of Presentation: ジャネリアファーム(米国)
  • Year and Date: 2010-03-23
• [Presentation] Two-photon uncaging microscope to study cerebral synapses in vitro and in vivo (2009)
  • Author(s): 河西春郎
  • Organizer: 第3回 Annual Nanobiotechnology Global Symposium
  • Place of Presentation: ロサンゼルス(米国)
  • Year and Date: 2009-11-18
• [Presentation] 新しい2光子刺激法の神経科学への応用 (2009)
  • Author(s): 河西春郎
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋(名古屋国際会議場)
  • Year and Date: 2009-09-16
• [Presentation] Two-photon uncaging in vitro and in vivo (2009)
  • Author(s): 河西春郎
  • Organizer: EMBO Practical course in "Two-photon imaging of brain cell dynamics"
  • Place of Presentation: Zurich(Swiss)
  • Year and Date: 2009-09-09
• [Presentation] Two-photon imaging of conformation changes in SNAP25 during insulin exocytosis from islet beta-cells, Symposium on exocytosis and neurons (2009)
  • Author(s): Kasai, H., Takahashi, N., Hatakeyama, H.
  • Organizer: Japanese Cell Biology Meeting
  • Place of Presentation: 名古屋(名古屋国際会議場)
  • Year and Date: 2009-06-02
• [Presentation] Patch-clamp, imaging and photostimulation (2009)
  • Author(s): 河西春郎
  • Organizer: Neurisons Conference 2010
  • Place of Presentation: ゲッティンゲン(ドイツ)
  • Year and Date: 2009-05-15
• [Presentation] SNARE蛋白の膜拡散 によるインスリン分泌調節の2光子顕微鏡に よる解析
  • Author(s): 大野光代 高橋倫子 河西春郎
  • Organizer: 第54回日本糖尿病 学会年次学術集会
  • Place of Presentation: 札幌
• [Presentation] Monitoring dynamics of single synapses during circuit plasticity
  • Author(s): Haruo Kasai
  • Organizer: IBRO summer course
  • Place of Presentation: Seoul
  • Invited
• [Presentation] SNARE conformation and insulin exocytosis
  • Author(s): Noriko Takahashi, Mitsuyo Ohno, Haruo Kasai
  • Organizer: 第54回日本糖尿病 学会年次学術集会シンポジウム
  • Place of Presentation: 札幌
  • Invited
• [Presentation] GABA dependence of synaptic competion in dendritic spines.
  • Author(s): Haruo Kasai
  • Organizer: Max-Planck-Institute/U-Tokyo Symposium
  • Place of Presentation: 東京
  • Invited
• [Presentation] Two-photon FRET/FLIM imaging of SNARE dependent exocytosis.
  • Author(s): Haruo Kasai, Mitsuyo Ohno, Wakako Sawada, Satoshi Watanabe, Noriko Takahashi
  • Organizer: IUPAB
  • Place of Presentation: Beijin (China)
  • Invited
• [Presentation] Synaptic competition in the dendritic spines of CA1 pyramidal neurons.
  • Author(s): Haruo Kasai
  • Organizer: 高等研国際シンポジウム
  • Place of Presentation: 奈良
  • Invited
• [Presentation] ２光子蛍光寿命画像法による開口放出SNARE機構の研究 (Two-photon FRET/FLIM imaging of SNARE dependent exocytosis)
  • Author(s): 高橋倫子、大野光代、澤田和可子、河西春郎
  • Organizer: 日本生理学会
  • Place of Presentation: 松本
• [Remarks]
  • URL: http://www.bm2.m.u-tokyo.ac.jp
• [Remarks]
  • URL: http://www.bm2.m.u-tokyo.ac.jp
• [Remarks] 河西研究室
  • URL: http://www.bm2.m.u-tokyo.ac.jp
• [Remarks]
  • URL: http://www.bm2.m.u-tokyo.ac.jp/
• [Remarks]
  • URL: http://www.bm2.m.u-tokyo.ac.jp/