| Budget Amount *help |
¥29,510,000 (Direct Cost: ¥22,700,000、Indirect Cost: ¥6,810,000)
Fiscal Year 2011: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2010: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2009: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
|
|---|
| Research Abstract |
We tried to establish a technique to generate highly brain region specific gene knockdown mouse. For this purpose, we used two types of transgenic mouse which express their transgenes in different manners in their brain. Amygdala is involved in fear memory. Because both CamKII and GRP are coexpressed only in the amygdala, we tried to generate amygdala specific gene knockdown mice using these genes’ promoters. We have generated transgenic mice which express miRNA that downregulate the NMDA receptor only in the amygdala. We are analyzing the expression level of the target gene in the mice.
|
