Establishment of a technique to generate highly brain region specific gene knockdown mouse and its application
| Project/Area Number |
21200006
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology and muscle physiology
Laboratory animal science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KIYAMA Yuji 東京大学, 医科学研究所, 助教 (90456195)
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| Research Collaborator |
OGAWA Itone 東京大学, 医科学研究所, 技術職員
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥29,510,000 (Direct Cost: ¥22,700,000、Indirect Cost: ¥6,810,000)
Fiscal Year 2011: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2010: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2009: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
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| Keywords | 扁桃体 / miRNA / NMDA受容体 / 恐怖 / 不安 / シナプス可塑性 / Cre-loxPシステム / プロモーター |
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| Research Abstract |
We tried to establish a technique to generate highly brain region specific gene knockdown mouse. For this purpose, we used two types of transgenic mouse which express their transgenes in different manners in their brain. Amygdala is involved in fear memory. Because both CamKII and GRP are coexpressed only in the amygdala, we tried to generate amygdala specific gene knockdown mice using these genes’ promoters. We have generated transgenic mice which express miRNA that downregulate the NMDA receptor only in the amygdala. We are analyzing the expression level of the target gene in the mice.
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Report
(4 results)
Research Products
(6 results)
