Molecular Biomechanics of Vascular Cell Mechano-Responses

• Project/Area Number: 21220011
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Dokkyo Medical University
• Principal Investigator: ANDO Joji 獨協医科大学, 医学部, 教授 (20159528)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Kimiko 東京大学, 大学院医学系研究科, 講師 (00323618)
• Project Period (FY): 2009-05-11 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥203,320,000 (Direct Cost: ¥156,400,000、Indirect Cost: ¥46,920,000); Fiscal Year 2013: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2012: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2011: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2010: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2009: ¥130,520,000 (Direct Cost: ¥100,400,000、Indirect Cost: ¥30,120,000)
• Keywords: 血管内皮細胞 / Shear stress / 血行力学因子 / メカノセンサー / カルシウム・シグナリング / 細胞膜 / アデノシン３リン酸 / 細胞分化 / shear stress / 形質膜 / 巨大単一膜ベシクル / メカノトランスダクション / lipid order / 膜流動性 / メカニカルストレス / メカノセンシング / ATP / カルシウムチャネル / イオンチャネル / ES細胞

Research Abstract

This study was undertaken to elucidate how vascular endothelial cells (ECs) sense shear stress generated by flowing blood and transmit the signal into the cell interior. The results demonstrated that shear stress rapidly decreases the lipid order of EC membranes and changes caveolar membrane domains from the liquid-ordered phase to liquid-disordered phase. A similar decrease in lipid order occurred in the artificial membrane of liposomes exposed to shear stress, suggesting that the membrane lipid order change is a physical phenomenon. Shear stress clearly increased the membrane fluidity in ECs. A novel imaging method developed in our laboratory revealed that the shear-stress-induced changes in membrane physical properties were linked to ATP release at caveolae that evoked a Ca2+ influx via P2X4 channels and a subsequent Ca2+ wave that propagated throughout the entire cell. These findings indicated that plasma membranes act as a shear stress sensor that triggers Ca2+ signaling in ECs.

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Research Products

• [Journal Article] Flow detection and calcium signaling in vascular endothelial cells (2013)
  • Author(s): J. Ando and K. Yamamoto
  • Journal Title: Cardiovasc. Res Volume: 99 Pages: 260-268
  • Peer Reviewed
• [Journal Article] Endothelial cell and model membranes respond to shear stress by rapidly decreasing the order of their lipid phases (2013)
  • Author(s): K. Yamamoto and J. Ando
  • Journal Title: J. Cell Sci Volume: 126 Pages: 1227-1234
  • Peer Reviewed
• [Journal Article] Flow detection and calcium signaling in vascular endothelial cells. (2013)
  • Author(s): J. Ando and K. Yamamoto
  • Journal Title: Cardiovasc. Res. Volume: 99 Issue: 2 Pages: 260-268
  • DOI: 10.1093/cvr/cvt084
  • Peer Reviewed
• [Journal Article] Endothelial cell and model membranes respond to shear stress by rapidly decreasing the order of their lipid phases. (2013)
  • Author(s): K. Yamamoto and J. Ando
  • Journal Title: J. Cell Sci. Volume: 126 Issue: 5 Pages: 1227-1234
  • DOI: 10.1242/jcs.119628
  • Peer Reviewed
• [Journal Article] Endothelial progenitor cells promote directional three-dimensional endothelial network formation by secreting vascular endothelial growth factor. (2013)
  • Author(s): Y. Abe, Y. Ozaki, J. Kasuya, K. Yamamoto, J. Ando, R. Sudo, M. Ikeda and K. Tanishita
  • Journal Title: PLoS ONE Volume: 8(12) Issue: 12 Pages: 1-12
  • DOI: 10.1371/journal.pone.0082085
  • Peer Reviewed
• [Journal Article] Dynamic imaging of flow-induced endothelial cell responses (2013)
  • Author(s): 山本希美子、安藤譲二
  • Journal Title: Japanese Journal of Thrombosis and Hemostasis Volume: 24 Issue: 6 Pages: 569-575
  • DOI: 10.2491/jjsth.24.569
  • NAID: 130003393319
  • ISSN: 0915-7441, 1880-8808
  • Peer Reviewed
• [Journal Article] Fluid shear stress induces differentiation of circulating phenotype endothelial progenitor cells. (2012)
  • Author(s): S. Obi, H. Masuda, T. Shizuno, A. Sato, K. Yamamoto, J. Ando, Y. Abe and T. Asahara
  • Journal Title: Am. J. Physiol. Cell Physiol. Volume: 303 Issue: 6 Pages: 595-606
  • DOI: 10.1152/ajpcell.00133.2012
  • Peer Reviewed
• [Journal Article] Arterial shear stress augments the differentiation of endothelial progenitor cells adhered to VEGF-bound surfaces. (2012)
  • Author(s): Y. Suzuki, K. Yamamoto, J. Ando, K. Matsumoto, T. Matsuda
  • Journal Title: Biochem. Biopys. Res. Commun. Volume: 423 Issue: 1 Pages: 91-97
  • DOI: 10.1016/j.bbrc.2012.05.088
  • Peer Reviewed
• [Journal Article] Differentiation of circulating endothelial progenitor cells induced by shear stress. (2012)
  • Author(s): S. Obi, K. Yamamoto, J. Ando, H. Masuda, T. Asahara.
  • Journal Title: 2012 International Symposium on Micro-NanoMechatronics and Human Science Volume: - Pages: 54-58
  • Peer Reviewed
• [Journal Article] Real-time imaging of flow-induced ATP release and Ca2+ signaling in vascular endothelial cells. (2012)
  • Author(s): K. Yamamoto and J. Ando.
  • Journal Title: Biorheology Volume: 49 Pages: 115-116
  • Peer Reviewed
• [Journal Article] 血管のメカノバイオロジー：血管内皮細胞の血流センシングと循環調節 (2012)
  • Author(s): 安藤 譲二、山本 希美子
  • Journal Title: 細胞工学 Volume: 31(6) Pages: 1017-1023
• [Journal Article] Visualization of flow-induced ATP release and triggering of Ca^<2+> waves at caveolae in vascular endothelial cells (2011)
  • Author(s): K. Yamamoto, K. Furuya, M. Nakamura, E. Kobatake, M. Sokabe, and J. Ando
  • Journal Title: J. Cell Sci Volume: 124 Pages: 3477-3483
  • Peer Reviewed
• [Journal Article] Effects of shear stress on the gene expression of endothelial nitricoxide synthase, endothelin-1, and tbronmomodulin in human retinal microvascular endothelial cells (2011)
  • Author(s): A.Ishibazawa, K.Yamamoto, J.Ando, et al
  • Journal Title: Invest.Ophthalmol.Vis.Sci. Volume: 52 Issue: 11 Pages: 8496-8504
  • DOI: 10.1167/iovs.11-7686
  • Peer Reviewed
• [Journal Article] New Molecular Mechanisms for Cardiovascular Disease:Blood Flow Sensing Mechanism in Vascular Endothelial Cells (2011)
  • Author(s): K.Yamamoto, J.Ando
  • Journal Title: Journal of Pharmacological Sciences Volume: 116 Issue: 4 Pages: 323-331
  • DOI: 10.1254/jphs.10R29FM
  • NAID: 10029895377
  • ISSN: 1347-8613, 1347-8648
  • Peer Reviewed
• [Journal Article] Visualization of flow-induced ATP release and triggering of Ca^<2+> waves at caveolae in vascular endothelial cells (2011)
  • Author(s): K.Yamamoto, J.Ando, et al
  • Journal Title: J.Cell Sci. Volume: 124 Issue: 20 Pages: 3477-3483
  • DOI: 10.1242/jcs.087221
  • Peer Reviewed
• [Journal Article] PGE_2-EP_2 receptor signaling in endothelium is activated by hemodynamic stress and induces cerebral aneurysm through an amplifying loop via NF-κB (2011)
  • Author(s): T.Aoki, K.Yamamoto, J.Ando, et al
  • Journal Title: Br.J.Pharmacol. Volume: 163 Issue: 6 Pages: 1237-1249
  • DOI: 10.1111/j.1476-5381.2011.01358.x
  • Peer Reviewed
• [Journal Article] Mechanosensing of blood flow in vascular endothelial cells (2011)
  • Author(s): 山本希美子、安藤譲二
  • Journal Title: Folia Pharmacologica Japonica Volume: 138 Issue: 5 Pages: 196-200
  • DOI: 10.1254/fpj.138.196
  • NAID: 10029896349
  • ISSN: 0015-5691, 1347-8397
  • Peer Reviewed
• [Journal Article] 血流刺激に対する内皮細胞応答とメカノセンシング機構 (2011)
  • Author(s): 山本希美子、安藤譲二
  • Journal Title: 細胞 Volume: 46(9) Pages: 339-343
• [Journal Article] Study on the sliding friction of endothelial cells cultured on hydrogel and the role of glycocalyx on friction reduction. (2010)
  • Author(s): Y.M Chen, et al.
  • Journal Title: Adv.Eng.Mater. Volume: 12
  • Peer Reviewed
• [Journal Article] Differentiation of stem/progenitor cells into vascular cells in response to fluid mechanical forces. (2010)
  • Author(s): K.Yamamoto, J.Ando
  • Journal Title: J.Biorheology Volume: 24 Pages: 1-10
  • NAID: 40017399300
  • Peer Reviewed
• [Journal Article] 血管内皮のカルシウム・シグナリングを介した血流感知機構 (2010)
  • Author(s): 山本希美子, 安藤譲二
  • Journal Title: 薬学雑誌 Volume: 130 Pages: 1401-1411
  • NAID: 130000451688
  • Peer Reviewed
• [Journal Article] 血流と内皮細胞機能 (2010)
  • Author(s): 山本希美子, 安藤譲二
  • Journal Title: THE LUNG Volume: 18 Pages: 286-290
  • NAID: 10016757242
• [Journal Article] 血管のメカノバイロジー (2010)
  • Author(s): 安藤譲二
  • Journal Title: 血管医学 Volume: 11 Pages: 29-37
• [Journal Article] シェアストレス (2010)
  • Author(s): 安藤譲二
  • Journal Title: 骨粗鬆症治療 Volume: 9 Pages: 69-72
• [Journal Article] 血流(ずり応力)による血管内皮細胞機能変化をみる (2010)
  • Author(s): 安藤譲二、山本希美子
  • Journal Title: Journal of Vascular Medicine 6 Pages: 155-122
• [Journal Article] (2010)
  • Author(s): 安藤譲二, 山本希美子
  • Journal Title: 血管内皮細胞とメカニカルストレス応答 循環器ストレス学(南山堂)
• [Journal Article] (2010)
  • Author(s): 安藤譲二, 山本希美子
  • Journal Title: Shear-stress-sensing and response mechanisms in vascular endothelial cells(Springer Interface Oral Health Science 2009)
• [Journal Article] Shear stress increases expression of the arterial endothelial markear ephrinB2 in murine ES cells via the VEGF-Notch signaling pathways (2009)
  • Author(s): T. Masumura, K. Yamamoto, N. Shimizu, S. Obi, and J. Ando
  • Journal Title: Arterioscler. Thromb. Vasc. Biol Volume: 29 Pages: 2125-2131
  • Peer Reviewed
• [Journal Article] Fluid shear stress induces arterial differentiation of endothelial progenitor cells (2009)
  • Author(s): S. Obi, K. Yamamoto, N. Shimizu, S. Kumagaya, T. Masumura, T. Sokabe, T. Asahara, and J. Ando
  • Journal Title: J. Appl. Physiol Volume: 106 Pages: 203-211
  • Peer Reviewed
• [Journal Article] Vascular Mechanobiology : Endothelial Cell Responses to Fluid Shear Stress (2009)
  • Author(s): 安藤譲二, 山本希美子
  • Journal Title: Cir.J. 73 Pages: 1983-1992
  • NAID: 10025936717
  • Peer Reviewed
• [Journal Article] Shear Stress Increases Expression of the Arterial Endothelial Marker EphrinB2 in Murine ES Cells via the VEGF-Notch Signaling Pathways (2009)
  • Author(s): T.Masumura, K.Yamamoto, et al.
  • Journal Title: Arteioscler.Throm.Vasc.Biol. 29 Pages: 2125-2131
  • Peer Reviewed
• [Journal Article] Contribution of rat endothelial progenitor cells on three-dimensional network formation in vitro (2009)
  • Author(s): M.Koga, et al.
  • Journal Title: Tissue Eng.Part A 15 Pages: 2727-2739
  • Peer Reviewed
• [Journal Article] Fluid shear stress induces arterial differentiation of endothelial progenitor cells (2009)
  • Author(s): S.Obi, K.Yamamoto, et al.
  • Journal Title: J.Appl.Physiol. 106 Pages: 203-211
  • Peer Reviewed
• [Journal Article] 血管内皮細胞へのシェアストレスと分子応答 (2009)
  • Author(s): 安藤譲二、山本希美子
  • Journal Title: 分子心血管病 10 Pages: 352-359
• [Presentation] Endothelial cells respond to shear stress by decreasing the lipid order of their plasma membranes (2013)
  • Author(s): K. Yamamoto
  • Organizer: 15^<th> International Conference on Biomedical Engineering
  • Place of Presentation: Singapore
  • Year and Date: 2013-12-06
• [Presentation] Endothelial cells respond to shear stress by decreasing the lipid order of their plasma membranes. (2013)
  • Author(s): K. Yamamoto and J. Ando
  • Organizer: 15th International Conference on Biomedical Engineering
  • Place of Presentation: Singapore
  • Invited
• [Presentation] Shear stress rapidly alters the physical properties of vascular endothelial cell membranes by decreasing their lipid order and increasing their fluidity (2013)
  • Author(s): K. Yamamoto, A. Kamiya and J. Ando
  • Organizer: 13th Mediterranean Conference on Medical and Biological Engineering and Computing
  • Place of Presentation: Seville, Spain
  • Invited
• [Presentation] 胚性幹細胞のメカノバイロジー (2013)
  • Author(s): 山本希美子 安藤譲二
  • Organizer: 第３１回日本ヒト細胞学会
  • Place of Presentation: 所沢市
  • Invited
• [Presentation] Shear stress increases differentiation of circulating endothelial progenitor cells via the VEGFR2/Pi3K/Akt/mTOR signaling (2013)
  • Author(s): S. Obi, K. Yamamoto, J. Ando, H. Masuda and T. Asahara
  • Organizer: 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: Osaka, Japan
• [Presentation] Vascular endothelial cells respond to shear stress by rapidly decreasing the order of their lipild phases (2013)
  • Author(s): K. Yamamoto and J. Ando
  • Organizer: 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: Osaka, Japan
  • Invited
• [Presentation] 血管細胞のバイロレオロジーと臨床医学の接点 岡小天賞受賞記念講演 (2013)
  • Author(s): 安藤譲二
  • Organizer: 第３６回日本バイオレオロジー学会
  • Place of Presentation: 福岡
  • Invited
• [Presentation] Vascular mechanobiology: flow detection and calcium signaling in endothelial cells (2013)
  • Author(s): K. Yamamoto and J. Ando
  • Organizer: Japan-Korea Joint Symposium 36th Annual Conference of Biorheology
  • Place of Presentation: 福岡
  • Invited
• [Presentation] Dynamic cellular mechanotransduction and nano-biomechanics (2013)
  • Author(s): K. Yamamoto and J. Ando
  • Organizer: The 8th Aso International Meeting
  • Place of Presentation: Kumamoto, Japan
  • Invited
• [Presentation] 血管内皮P2X4受容体を介した循環機能調節 (2013)
  • Author(s): 山本希美子 安藤譲二
  • Organizer: 第８６回日本薬理学会年会
  • Place of Presentation: 福岡
  • Invited
• [Presentation] Visualization of flow-induced ATP release and Ca^<2+> waves in vascular endothelial cells (2012)
  • Author(s): J. Ando
  • Organizer: ISACB 13^<th> Biennial Meeting
  • Place of Presentation: London
  • Year and Date: 2012-09-13
• [Presentation] Shear stress mechanotransduction via ATP receptors in vascular endothelial cells (2012)
  • Author(s): K.Yamamoto, J.Ando
  • Organizer: International Symposium on Cellular Mechanobiology
  • Place of Presentation: 京都大学百周年時計台記念館(京都府京都市)(招待講演)
  • Year and Date: 2012-03-16
• [Presentation] 血管内皮におけるシェアストレスのメカノトランスダクションと循環調節 (2012)
  • Author(s): 山本希美子、安藤譲二
  • Organizer: 日本機械学会第24回バイオエンジニアリング部門講演会
  • Place of Presentation: 大阪大学豊中キャンパス(大阪府豊中市)(招待講演)
  • Year and Date: 2012-01-08
• [Presentation] 血管メカノバイオロジーと臨床医学の接点 (2012)
  • Author(s): 安藤譲二
  • Organizer: 第１４回徳島臨床脈管研究会
  • Place of Presentation: 徳島
  • Invited
• [Presentation] Differentiation of circulating endothelial progenitor cells induced by shear stress (2012)
  • Author(s): S. Obi, K. Yamamoto, J. Ando, H. Masuda and T. Asahara
  • Organizer: 23rd International Symposium on Micro-NanoMechantronics and Human Science
  • Place of Presentation: Nagoya
• [Presentation] 血管のメカノバイロジー：血流に対する内皮細胞応答と血管リモデリング (2012)
  • Author(s): 安藤譲二
  • Organizer: 第32回日本川崎病学会 特別講演
  • Place of Presentation: 東京
  • Invited
• [Presentation] Chemiluminescence imaging of flow-induced ATP release at caveolae in vascular endothelial cells. (2012)
  • Author(s): K. Yamamoto and J. Ando
  • Organizer: 第５０回日本生物物理学会
  • Place of Presentation: 名古屋
  • Invited
• [Presentation] Vascular cell responses to biomechanical forces (2012)
  • Author(s): K. Yamamoto and J. Ando
  • Organizer: 9th International Conference on Flow Dynamics
  • Place of Presentation: Sendai
  • Invited
• [Presentation] Visualization of flow-induced ATP release and Ca2+ waves in vascular endothelial cells. (2012)
  • Author(s): J. Ando and K. Yamamoto
  • Organizer: ISACB 13th Biennial Meeting
  • Place of Presentation: London
• [Presentation] 血管内皮細胞による血流センシングと循環調節 (2012)
  • Author(s): 安藤譲二
  • Organizer: 第９回日本病理学会カンファレンス
  • Place of Presentation: 山口県湯田温泉
  • Invited
• [Presentation] 血管のメカノバイオロジー：内皮細胞とシェアストレス (2012)
  • Author(s): 安藤譲二
  • Organizer: トランスレーショナル・リサーチ・セミナー IN DOKKYO
  • Place of Presentation: 獨協医科大学 栃木県壬生町
  • Invited
• [Presentation] Shear-stress-mediated control of vascular functions through endothelial ATP release and P2X4 ion channels. (2012)
  • Author(s): K. Yamamoto and J. Ando
  • Organizer: 14th International Congress of Biorheology and 7th International Conference on Clinical Hemorheology
  • Place of Presentation: Istanbul
  • Invited
• [Presentation] ATP-gated P2X4 ion channels serve as transducers for shear stress mechanotransduction in vascular endothelial cells. (2012)
  • Author(s): K. Yamamoto and J. Ando
  • Organizer: International Symposium on Purinergic Signaling in New Strategy of Drug Discovery
  • Place of Presentation: Fukuoka
  • Invited
• [Presentation] Visualization of flow-induced ATP release and triggering of Ca2+ waves at caveolae in vascular endothelial cells. (2012)
  • Author(s): K. Yamamoto and J. Ando
  • Organizer: World Congress of Medical Physics and Biomedical Engineering
  • Place of Presentation: Beijing
• [Presentation] 血流を介した血管システムの制御機構 (2012)
  • Author(s): 安藤譲二
  • Organizer: 理研「水科学」ワークショップ
  • Place of Presentation: 和光市
  • Invited
• [Presentation] 血管のメカノバイオロジー：血流刺激に対する内皮細胞応答 (2012)
  • Author(s): 山本希美子 安藤譲二
  • Organizer: 第５１回日本生体医工学学会
  • Place of Presentation: 福岡
  • Invited
• [Presentation] Fluid shear stress induces ATP release and triggering Ca^<2+> waves at caveolae in vascular endothelial cells (2011)
  • Author(s): 山本希美子、安藤譲二
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)(招待講演)
  • Year and Date: 2011-12-16
• [Presentation] Real-time-imaging of shear-stress-induced ATP release and trigering of Ca^<2+> waves in vascular endothelial cells (2011)
  • Author(s): K.Yamamoto, J.Ando
  • Organizer: 第19回日本血管生物医学会学術集会
  • Place of Presentation: 東京ステーションコンファレンス(東京都千代田区)(招待講演)
  • Year and Date: 2011-12-10
• [Presentation] シェアストレスが血管内皮増殖因子固定表面に接着した血管内皮前駆細胞の分化過程に与える影響 (2011)
  • Author(s): 鈴木芳典、山本希美子、安藤譲二, 他2名
  • Organizer: 平成23年度日本生体医工学会北陸支部大会
  • Place of Presentation: 金沢大学サテラィトプラザ(石川県金沢市)
  • Year and Date: 2011-12-10
• [Presentation] Differential mechanism of mechanical stress in circulating endothelial progenitor cells (2011)
  • Author(s): Syotaro Obi, K.Yamamoto, J.Ando, et al
  • Organizer: 第19回日本血管生物医学会学術集会
  • Place of Presentation: 東京ステーションコンファレンス(東京都千代田区)
  • Year and Date: 2011-12-10
• [Presentation] 血管内皮におけるP2X4イオンチャネルを介した流れずり応力のメカノトランスダクションと循環調節 (2011)
  • Author(s): 山本希美子、安藤譲二
  • Organizer: 生理学研究所研究会
  • Place of Presentation: 生理研究所岡崎コンファレンスセンター(愛知県岡崎市)(招待講演)
  • Year and Date: 2011-11-30
• [Presentation] Endothelial mechanotransduction and its role in the control of vascular functions (2011)
  • Author(s): K.Yamamoto, J.Ando
  • Organizer: Intemational Symposium on Mechanobiology, The 5^<th> Shanghai International Conference on Biophysics and Molecular Biology
  • Place of Presentation: Shanghai Huashia Hotel (Shanghai, China)(招待講演)
  • Year and Date: 2011-11-06
• [Presentation] Shear-stress-mediated control of vascular functions through endothelial ATP release and P2X4 receptors (2011)
  • Author(s): K.Yamamoto, J.Ando
  • Organizer: International Symposium on Mechanobiology JSPS-NOW Joint Scminsr
  • Place of Presentation: 昭和薬科大学(東京都町田市)(招待講演)
  • Year and Date: 2011-11-04
• [Presentation] Shear-stress-mediated control of vascular functions through endothelial ATP release and P2X4 ion channels (2011)
  • Author(s): J. Ando
  • Organizer: 9^<th> International Congress on Coronary Artery Disease
  • Place of Presentation: Venice, Italy
  • Year and Date: 2011-10-24
• [Presentation] Shear-stress-mediated control of vascular functions through endothelial ATP release and P2X4 ion channels (2011)
  • Author(s): Joji Ando, K.Yamamoto
  • Organizer: 9^<th> International Congress on Coronary Artery Disease (ICCAD)
  • Place of Presentation: Convention Center (Venice, Itaiy)
  • Year and Date: 2011-10-10
• [Presentation] Shear Stress Mechanotransduction via Endogenous ATP Release in Vascular Endothelial Cells (2011)
  • Author(s): K.Yamamoto, J.Ando
  • Organizer: The 5^<th> European Conference of the International Federation for Medical and Biomedical Engineering
  • Place of Presentation: Budapest Congreee & World Trade Center (Budapest, Hungary)
  • Year and Date: 2011-09-16
• [Presentation] 流れずり応力による循環内皮前駆細胞の分化誘導 (2011)
  • Author(s): 小尾正太郎、山本希美子、安藤譲二, 他3名
  • Organizer: 第34回日本バイオレオロジー学会年会
  • Place of Presentation: 関西大学100周年記念会館(大阪府吹田市)
  • Year and Date: 2011-06-03
• [Presentation] 生体力学刺激によるES細胞の血管細胞への分化誘導 (2011)
  • Author(s): 山本希美子、安藤譲二
  • Organizer: 第50回日本生体医工学会大会
  • Place of Presentation: 東京電機大学神田キャンパス(東京都千代田区)(招待講演)
  • Year and Date: 2011-04-29
• [Presentation] 流れずり応力が内皮前駆細胞の分化に及ぼす効果 (2011)
  • Author(s): 小尾正太郎、山本希美子、安藤譲二, 他3名
  • Organizer: 第50回日本生体医工学会大会
  • Place of Presentation: 東京電機大学神田キャンパス(東京都千代田区)
  • Year and Date: 2011-04-29
• [Presentation] Shear stress mechanotransduction and its role in the control of blood pressure (2010)
  • Author(s): K.Yamamoto, J.Ando
  • Organizer: Biochemistry and Molecular Biology 2010
  • Place of Presentation: 神戸ポートピアアイランド(兵庫県神戸市)
  • Year and Date: 2010-12-07
• [Presentation] ATP-gated P2X4 ion channel serve as transducers for shear stress mechanotransduction in vascular endothelial cells (2010)
  • Author(s): K.Yamamoto, J.Ando
  • Organizer: Symposium, 8^<th> Korea-Japan Joint Symposium on Vascular Biology, 18^<th> Annual Meeting of the Japanese Vascular Biology and Medicine
  • Place of Presentation: 梅田スカイビル(大阪府大阪市)
  • Year and Date: 2010-12-03
• [Presentation] 流れ刺激が循環血管内皮前駆細胞に及ぼす効果 (2010)
  • Author(s): 小尾正太郎, 他
  • Organizer: 第18回日本血管生物医学会
  • Place of Presentation: 梅田スカイビル(大阪府大阪市)
  • Year and Date: 2010-12-01
• [Presentation] 近未来の医療を開く最先端の血管研究:血流によるメカニカルストシスと血管内皮による循環調節 (2010)
  • Author(s): 安藤譲二
  • Organizer: 旭川医科大学学術講演会
  • Place of Presentation: 旭川医科大学(北海道旭川市)
  • Year and Date: 2010-11-18
• [Presentation] Endothelial ATP receptor-mediated shear-stress-signal transduction and its physiological roles. (2010)
  • Author(s): K.Yamamoto, J.Ando
  • Organizer: 6^<th> World Congress of Biomechanics
  • Place of Presentation: Singapore Suntec Convention Centre(Singapore)
  • Year and Date: 2010-08-05
• [Presentation] 細胞表面結合性ルシフェラーゼタンパクを用いたATP放出現象のリアルタイムイメージング (2010)
  • Author(s): 山本希美子, 安藤譲二
  • Organizer: 第49回日本生体医工学学会大会
  • Place of Presentation: 大阪国際交流センター(大阪府大阪市)
  • Year and Date: 2010-06-25
• [Presentation] 流れ誘発性細胞膜相転移の蛍光ライブイメージング (2010)
  • Author(s): 山本希美子, 安藤譲二
  • Organizer: 第49回日本生体医工学学会大会
  • Place of Presentation: 大阪国際交流センター(大阪府大阪市)
  • Year and Date: 2010-06-25
• [Presentation] 血流力学刺激によるES細胞の分化誘導に増殖因子受容体の活性化が関与する (2010)
  • Author(s): 桝村智美, 山本希美子, 安藤譲二
  • Organizer: 第49回日本生体医工学学会大会
  • Place of Presentation: 大阪国際交流センター(大阪府大阪市)
  • Year and Date: 2010-06-25
• [Presentation] Biomedical force-induced differentiation of ES cells into vascular cells. Symposium on Mechano-transduction and vascular biology (2010)
  • Author(s): J. Ando
  • Organizer: XX World Congress of International Society for Heart Research
  • Place of Presentation: Kyoto
  • Year and Date: 2010-05-16
• [Presentation] Biomedical force-induced differentiation of ES cells into vascular cells (2010)
  • Author(s): J.Ando, K.Yamamoto
  • Organizer: XX World Congress of International Society for Heart Research, Symposium on Mechano-transduction and vascular biology
  • Place of Presentation: 京都国際会館(京都府京都市)
  • Year and Date: 2010-05-16
• [Presentation] 血管内皮のカルシウムシグナリングを介した血流感知機構 (2010)
  • Author(s): 山本希美子、安藤譲二
  • Organizer: 第130回日本薬学会年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-28
• [Presentation] 血管内皮細胞における血流感知機構 (2010)
  • Author(s): 山本希美子、安藤譲二
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-16
• [Presentation] 内皮細胞のシェアストレス応答と循環機能調節 (2009)
  • Author(s): 安藤譲二
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Shear stress induced arterial differentiation of murine ES cells (2009)
  • Author(s): 桝村智美, 山本希美子, et al.
  • Organizer: MHS2009
  • Place of Presentation: Nagoya
  • Year and Date: 2009-11-10
• [Presentation] Shear stress induces differentiation of arterial endothelial cells from murine embryonic stem cells (2009)
  • Author(s): 山本希美子, et al.
  • Organizer: Medical Physics and Biomedical Engineering, World Congress 2009
  • Place of Presentation: Munich
  • Year and Date: 2009-11-08
• [Presentation] 血流刺激による血管細胞の分化誘導 (2009)
  • Author(s): 山本希美子、安藤譲二
  • Organizer: 第17回日本血管生物医学会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-09
• [Presentation] 流れずり応力によるVEGF-Notchシグナルを介したマウス胚性幹細胞におけるephrinB2の発現上昇 (2009)
  • Author(s): 桝村智美、山本希美子、安藤譲二
  • Organizer: 第17回 日本血管生物医学会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-09
• [Presentation] Fluid-mechanical force-induced differentiation of murine ES cells towards vascular cells (2009)
  • Author(s): 山本希美子, et al.
  • Organizer: The Third Switzerland-Japan Workshop on Biomechanics 2009
  • Place of Presentation: Engelberg
  • Year and Date: 2009-09-04
• [Presentation] Shear-dependent mechanotransduction via endothelial ATP receptors and its physiological role in the vascular system (2009)
  • Author(s): J. Ando
  • Organizer: 36^<th> International Congress of Physiological Sciences (IUPS2009)
  • Place of Presentation: Kyoto
  • Year and Date: 2009-07-31
• [Presentation] Shear Stress Mechanotransduction via Endothelial ATP Receptors and its Physiolo gical Role in the Vascular System (2009)
  • Author(s): 安藤譲二, 山本希美子
  • Organizer: The 36^<th> International Congress of Physiological Science
  • Place of Presentation: Kyoto
  • Year and Date: 2009-07-31
• [Presentation] Endothelial P2X4-mediated shear-stress-mechanotransduction and its roles in the control of vascular functions (2009)
  • Author(s): 山本希美子, 安藤譲二
  • Organizer: Fukuoka Purine 2009, Joint with JSPS Core-to-Core Program, A Satellite Symposium for IUPS 2009
  • Place of Presentation: Fukuoka
  • Year and Date: 2009-07-23
• [Presentation] Shear stress によるVEGF-Notchシグナルを介したES細胞の動脈化 (2009)
  • Author(s): 桝村智美、山本希美子, 他3名
  • Organizer: 第32回 日本バイオレオロジー学会年会
  • Place of Presentation: 桐生
  • Year and Date: 2009-06-04
• [Presentation] 流れずり応力が惹起する内皮細胞膜特性の変化 (2009)
  • Author(s): 山本希美子、神谷瞭、安藤譲二
  • Organizer: 第48回日本生体医工学学会大会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-23
• [Presentation] 流れ剪断応力はVEGF-Notchシグナルを介して血管内皮前駆細胞を動脈化する (2009)
  • Author(s): 桝村智美、山本希美子, 他3名
  • Organizer: 第48回日本生体医工学学会大会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-23
• [Book] Endothelial mechanotransduction and its role in the control of vascular functions. (2012)
  • Author(s): K. Yamamoto and J. Ando
  • Publisher: Shanghai Scientific and Technological Literature Publishing House.
• [Book] Molecular Mechanisms Underlying Mechanosensing in Vascular Biology in "Mechanosensing Biology" (2011)
  • Author(s): K.Yamamoto, J.Ando
  • Total Pages: 17
  • Publisher: Springer
• [Book] Shear-stress-sensing and response mechanisms in vascular endothelial cells. IN "Interface Oral Health Science 2009" (2010)
  • Author(s): J.Ando, K.Yamamoto
  • Total Pages: 7
  • Publisher: Springer
• [Remarks]
  • URL: http://www.dokkyomed.ac.jp/dep-m/biomech/
• [Remarks] 獨協医科大学医学部・生体医工学・安藤研究室
  • URL: http://www.dokkyomed.ac.jp/dep-m/biomech/
• [Remarks] 獨協医科大学医学部・生体医工学・安藤研究室
  • URL: http://www.dokkyomed.ac.jp/dep-m/biomech/