| Project/Area Number |
21229003
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Osaka University |
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Principal Investigator |
YASUSHI Okamura 大阪大学, 医学(系)研究科(研究院), 教授 (80201987)
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| Co-Investigator(Kenkyū-buntansha) |
SAKIMURA Kenji 新潟大学, 脳研究所, 教授 (40162325)
YOSHIDA Manabu 東京大学, 大学院理学系研究科, 准教授 (60301785)
NAKAGAWA Atsushi 大阪大学, たんぱく質研究所, 教授 (20188890)
FUJIWARA Yuichiro 大阪大学, 大学院医学系研究科, 准教授 (20532980)
TSUTSUI Hidekazu 大阪大学, 大学院医学系研究科, 特任講師 (30392038)
OUKOUCHI Yoshifumi 大阪大学, 大学院医学系研究科, 助教 (90435818)
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| Co-Investigator(Renkei-kenkyūsha) |
MIYAWAKI Atsushi 理化学研究所, 細胞機能探索技術開発チーム, シニア・リーダー (80251445)
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| Project Period (FY) |
2009-05-11 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥143,130,000 (Direct Cost: ¥110,100,000、Indirect Cost: ¥33,030,000)
Fiscal Year 2012: ¥32,240,000 (Direct Cost: ¥24,800,000、Indirect Cost: ¥7,440,000)
Fiscal Year 2011: ¥31,460,000 (Direct Cost: ¥24,200,000、Indirect Cost: ¥7,260,000)
Fiscal Year 2010: ¥35,490,000 (Direct Cost: ¥27,300,000、Indirect Cost: ¥8,190,000)
Fiscal Year 2009: ¥43,940,000 (Direct Cost: ¥33,800,000、Indirect Cost: ¥10,140,000)
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| Keywords | 生体膜 / チャネル / トランスポーター / 能動輸送 / 電位センサー / プロトン / 膜電位 / ホスホイノシチド |
|---|
| Research Abstract |
Molecular mechanisms of voltage sensor domain proteins, including voltage-sensing phosphatase and voltage-gated proton channels, were studied by X-ray crystallography, electrophysiology, fluorescence imaging or fluorometry. We also exploited advantages of molecular mechanisms to develop efficient voltage-reporter protein and applied them in zebrafish heart and mouse auditory cortex and were convinced that these probes serve as efficient reporter. Knock out mice were establsihed for VSP, VSOP/Hv1 and VSOP2 and physiological functions of these voltage-signaling molecules have been revealed.
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| Assessment Rating |
Verification Result (Rating)
A
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