Elucidating mechanisms of embryonic cerebellar development

• Project/Area Number: 21240027
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Nagoya University
• Principal Investigator: MIYATA Takaki 名古屋大学, 医学系研究科, 教授 (70311751)
• Co-Investigator(Kenkyū-buntansha): KAWAGUCHI Ayano 名古屋大学, 大学院・医学系研究科, 准教授 (90360528) ; SAKAKIBARA Akira 名古屋大学, 大学院・医学系研究科, 助教 (20510217) ; HASHIMOTO Mitsuhiro 名古屋大学, 大学院・医学系研究科, 特任助教 (90311357)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥39,910,000 (Direct Cost: ¥30,700,000、Indirect Cost: ¥9,210,000); Fiscal Year 2012: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000); Fiscal Year 2010: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000); Fiscal Year 2009: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
• Keywords: 発生 / 発達 / 再生神経科学 / 小脳 / プルキンエ細胞 / 神経前駆細胞 / 細胞分裂 / 細胞移動 / 皮質形成 / 細胞運命決定 / 移動 / 分化 / 層形成 / 細胞周期 / 非対称分裂 / リーリン / 極性

Research Abstract

This study aimed at elucidating mechanisms of cerebellar development. Special attentions were paid on the behavior of young Purkinje cells and their progenitor cells. Understanding of how Purkinje cells migrate and form a layer in the cerebellar cortex is important for dissecting pathogenesis of human cerebellar malformations. We visualized newly-generated Purkinje cells’ morphology and dynamics for the first time and the results were published. Cell cycle parameters and lineage of progenitor cells that give rise to Purkinje cells were also studied.

Research Products

• [Journal Article] Dynamics of centrosome translocation and microtubule organization in neocortical neurons during distinct modes of polarization (2013)
  • Author(s): Sakakibara, A., Sato, T., Ando, R., Noguchi, N., Masaoka, M., Miyata, T
  • Journal Title: Cereb. Cortex Volume: - Issue: 5 Pages: 1301-1310
  • DOI: 10.1093/cercor/bhs411
  • Peer Reviewed
• [Journal Article] Dynamics of Centrosome Translocation and Microtubule Organization in Neocortical Neurons during Distinct Modes of Polarization. (2013)
  • Author(s): Sakakibara A
  • Journal Title: Cereb Cortex Volume: 2013
  • Peer Reviewed
• [Journal Article] Reelin-dependent ApoER2 downregulation uncouples newborn neurons from progenitor cells (2012)
  • Author(s): Perez-Martinez, F.J., Luque-Rio, A., Sakakibara, A., Hattori, M., Miyata, T., Luque, J. M.
  • Journal Title: Biol. Open Volume: 1 Pages: 1258-1263
  • Peer Reviewed
• [Journal Article] Reelin-dependent ApoER2 downregulation uncouples newborn neurons from progenitor cells. (2012)
  • Author(s): Perez-Martinez, F.J., Luque-Rio, A.,Sakakibara, A., Hattori, M., Miyata, T., Luque, J. M.
  • Journal Title: Biol Open Volume: 1 Pages: 1258-1263
  • Peer Reviewed
• [Journal Article] Local application of neurotrophins speciffies axons through inositol 1, 4, 5-trisphosphate, calcium, and Ca2+/calmodulin-dependent protein kinases (2011)
  • Author(s): Shinichi Nakamuta, Yasuhiro Funahashi, Takashi Namba, Nariko Arimura, Marina R.Picciotto, Hiroshi Tokumitsu, Thomas R.Soderling, Akira Sakakibara, Takaki Miyata, Hiroyuki Kamiguchi, Kozo Kaibuchi
  • Journal Title: Science Signaling Volume: 4 Pages: 1-13
  • Peer Reviewed
• [Journal Article] Migration, early axonogenesis, and Reelin-dependent layer-forming behavior of early/posterior-born Purkinje cells in the developing mouse lateral cerebellum. (2010)
  • Author(s): Miyata, T., Ono Y, Okamoto M, Masaoka M, Sakakibara A, Kawaguchi A, Hashimoto M, Ogawa M.
  • Journal Title: Neural Dev. Volume: 5 Pages: 23-23
  • Peer Reviewed
• [Journal Article] Migration, early axonogenesis, and Reelin-dependent layer-forming behavior of early/posterior-born Purkinje cells in the developing mouse lateral cerebellum. (2010)
  • Author(s): Miyata T, Ono Y, Okamoto M, Masaoka M, Sakakibara A, Kawaguchi A, Hashimoto M, Ogawa M.
  • Journal Title: Neural Development. Volume: Vol.5
  • Peer Reviewed
• [Journal Article] Downregulation of functional Reelin receptors in projection neurons implies that primary Reelin action occurs at early/premigratory stages (2009)
  • Author(s): Uchida, T., ほか
  • Journal Title: J.Neuroscience 29 Pages: 10653-10662
  • Peer Reviewed
• [Presentation] Cohots of PCs that share the same birthdate communicate each other and form individual compartments in the cerebellum (2013)
  • Author(s): M.Hashimoto, T.Miyata
  • Organizer: The 23rd CDB Meeting Building multicellular systems from cellular cross-talk
  • Place of Presentation: 理化学研究所 発生・再生科学総合研究センター(神戸市
• [Presentation] 小脳プルキンエ細胞を生み出す前駆細胞の挙動についての研究 (2013)
  • Author(s): 宮田卓樹、三輪貴之、榊原明、川口綾乃、橋本光広
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(名古屋市熱田区)
• [Presentation] Cohots of PCs that share the same birthdate communicate each other and form individual compartments in the cerebellum. (2013)
  • Author(s): M.Hashimoto, T.Miyata
  • Organizer: The 23rd CDB Meeting Building multicellular systems from cellular cross-talk
  • Place of Presentation: 理化学研究所 発生・再生科学総合研究センター(神戸市中央区)
• [Presentation] 小脳プルキンエ細胞を生み出す前駆細胞の挙動についての研究 (2012)
  • Author(s): 宮田卓樹、三輪貴之、榊原明、川口綾乃、橋本光広:ポスター
  • Organizer: 第35回日本神経科学大会 Neuro2012
  • Place of Presentation: 名古屋国際会議場(名古屋市)
• [Presentation] 神経分化過程における細胞の運命決定相互作用:誕生直後の娘細胞の近隣関係性のイメージング (2011)
  • Author(s): 岡本麻友美, 佐合健, 藤森俊彦, 宮田卓樹
  • Organizer: 日本神経科学会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-09-16
• [Presentation] 哺乳類の脳形成を深く理解するためにできるだけ多くの細胞たちを見つめる (2011)
  • Author(s): 宮田卓樹, 榊原明, 岡本麻友美, 佐合健, 佐藤俊之, 川口綾乃
  • Organizer: 日本発生生物学会
  • Place of Presentation: 沖縄県宜野湾市
  • Year and Date: 2011-05-20
• [Presentation] Studying nascent cells' neighborship to better understand the mechanism of cell-fate determination in neocortical neurogenesis (2011)
  • Author(s): Mayumi Okamoto, Ken Sagou, Takaki Miyata
  • Organizer: The Cambridge Neural Stem Cell Symposium
  • Place of Presentation: 英国Cambridge
• [Presentation] Corticogenesis (2010)
  • Author(s): Takaki Miyata
  • Organizer: 理研脳科学総合研究センターチュートリアル(英語)
  • Place of Presentation: 和光市
  • Year and Date: 2010-11-09
• [Presentation] Links between neuronal migration, layer formation, and wiring in the developing mammalian cerebrum and cerebellum (2010)
  • Author(s): Takaki Miyata
  • Organizer: SFBセミナー
  • Place of Presentation: ドレスデン独立行政法人
  • Year and Date: 2010-09-04
• [Presentation] Migration and layer-initiating behavior of Purkinje cells (2010)
  • Author(s): Takaki Miyata
  • Organizer: 第33回日本神経科学大会(Neuro2010)
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-04
• [Presentation] Links between neuronal migration, layer formation, and wiring in the developing mammalian cerebrum and cerebellum (2010)
  • Author(s): Takaki Miyata
  • Organizer: SFB セミナー2010
  • Place of Presentation: Dresden
  • Year and Date: 2010-07-19
• [Presentation] 小脳皮質におけるリーリンの役割 (2010)
  • Author(s): 宮田卓樹
  • Organizer: 第50回日本先天異常学会学術集会
  • Place of Presentation: 淡路島
  • Year and Date: 2010-07-08
• [Presentation] マウス小脳原基における細胞周期に関する研究 (2010)
  • Author(s): 三輪貴之,伊藤恵美,太田久美子,榊原明,川口綾乃,宮田卓樹:ポスター
  • Organizer: 第33回日本神経科学大会 Neuro2010
  • Place of Presentation: 神戸コンベンションセンター(神戸市)
• [Presentation] Cerebeller development: from cytogenesis and layering to topographic circuit formation, Migration and layer-initiating behavior of Purkinje cells (2010)
  • Author(s): 宮田,宮田卓樹
  • Organizer: 第33回日本神経科学大会 Neuro2010
  • Place of Presentation: 神戸コンベンションセンター(神戸市)
• [Presentation] Observation of cellular behaviors that form the Purkinje plate in the embryonic cerebellum (2009)
  • Author(s): 宮田卓樹, 榊原明, 岡本麻友美, 川口綾乃, 小川正晴
  • Organizer: 米国神経科学会
  • Place of Presentation: 米国シカゴ
  • Year and Date: 2009-10-19
• [Presentation] Cortical plate and Purkinje plate : how do they form? (2009)
  • Author(s): 宮田卓樹, 榊原明, 佐藤俊之, 小川正晴
  • Organizer: 国際シンポジウム「construction and reconstruction of the brain」
  • Place of Presentation: 淡路島
  • Year and Date: 2009-10-08
• [Presentation] Behaviors of nascent Purkinje cells in the developing mouse cerebellum (2009)
  • Author(s): 宮田卓樹, 正岡実, 小川正晴
  • Organizer: 日本神経科学会
  • Place of Presentation: 名古屋市
  • Year and Date: 2009-09-18
• [Presentation] Birth, migration, and polarization of Purkinje cells in the mouse cerebellum (2009)
  • Author(s): 宮田卓樹
  • Organizer: 日本発生生物学会
  • Place of Presentation: 新潟市
  • Year and Date: 2009-05-29
• [Book] 神経系発生過程における細胞移動 「再生医療叢書:神経系」 (2013)
  • Author(s): 宮田卓樹
  • Publisher: 朝倉書店
• [Book] 小脳皮質形成メカニズムの解明をめざして:プルキンエ細胞の移動と配置の過程に関する新知見 ブレインサイエンスレビュー2011 (2011)
  • Author(s): 宮田卓樹
• [Remarks]
• [Remarks] 中日新聞に論文内容の紹介(H22.10.4.カラー図つきで)
• [Remarks] 研究室ホームページにて日本語で解説
  • URL: http://www.med.nagoya-u.ac.jp/dev-bio/research/meaar_paper_info.html
• [Remarks] 研究室ホームページにて日本語で解説
  • URL: http://www.med.nagoya-u.ac.jp/dev-bio/research/meaar_paper_info.html
• [Remarks] 発表先電子ジャーナルからのダウンロード(無料・自由)のためのアクセス
  • URL: http://www.neuraldevelopment.com/content/5/1/23
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/dev-bio/index.html