| Project/Area Number |
21241053
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
SEKINE Mitsuo 東京工業大学, 大学院・生命理工学研究科, 教授 (40111679)
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| Co-Investigator(Kenkyū-buntansha) |
OHKUBO Akihiro 東京工業大学, 大学院・生命理工学研究科, 助教 (60376960)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
Fiscal Year 2011: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2010: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
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| Keywords | 生体高分子 / RNA合成 / 筋ジストロフィー / 2'水酸基の保護基 / RNA医薬 / Michael反応 / シアノエチル基 / アミダイトユニット / MCE基 / 固相合成法 / 2'水酸某の保護基 / 塩基部無保護 / RNAの化学合成 / マイケル反応 / 2'-保護基 / シアノエチル化 / 脱アミノ化反応 / 合成モノマーユニット / アデノシン / グアノシン |
|---|
| Research Abstract |
An innovative method for the highly 2'-O-selective modification of ribonucleoside derivatives with cyanoethyl and 2-(N-methylcarbamoyl) ethyl(MCE) groups by Michael reaction has been developed. Various 2'-O-modified RNA derivatives were synthesized by using this new method. Extensive studies using these synthetic RNA derivatives showed they had more effective exon-skipping activities that indicate the efficacy as drugs for Duchenne muscular dystrophy.
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