Analysis of dynamic structure-function network of heterochromatin

• Project/Area Number: 21247001
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Hokkaido University
• Principal Investigator: MURAKAMI Yota 北海道大学, 大学院・理学研究院, 教授 (20260622)
• Co-Investigator(Kenkyū-buntansha): TANAKA Katsunori 関西学院大学, 理工学部, 教授 (60273926)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥43,160,000 (Direct Cost: ¥33,200,000、Indirect Cost: ¥9,960,000); Fiscal Year 2011: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000); Fiscal Year 2010: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000); Fiscal Year 2009: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
• Keywords: ヘテロクロマチン / RNAi / 転写 / 非コードRNA / RNAポリメラーゼII / 分裂酵母 / Two hybrid法

Research Abstract

Heterochromatin is an important for epigenetic gene regulation and maintenance of genetic information. Using fission yeast as a model organism, we focused on RNAi-directed heterochromatin formation and tried to understand the molecular mechanism of its formation and function. We revealed a part of network of transcription factors that regulate the formation and function of heterochromatin. In addition, we also presented a novel concept : factory for RNAi-directed heterochromatin formation is formed at nuclear periphery.

Research Products

• [Journal Article] Heterochromatin protein 1 homologue Swi6acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly (2012)
  • Author(s): Hayashi A., Ishida M., Kawaguchi R., Urano T., Murakami Y. and Nakayama J-I.
  • Journal Title: Proc. Natl. Acad. Sci. Volume: 109 Pages: 6159-6164
• [Journal Article] DNA-RNAhybrid formation mediates RNAi-directed heterochromatin formation (2012)
  • Author(s): Nakama, M., Kawakami, K., Kajitaini, T., Urano, T. and Murakami, Y.
  • Journal Title: Genes to Cells Volume: 17 Pages: 218-233
• [Journal Article] DNA-RNA hybrid formation mediates RNAi-directed heterochromatin formation (2012)
  • Author(s): Nakama, M., Kawakami, K., Kajitaini, T., Urano, T., Murakami, Y
  • Journal Title: Genes to Cells Volume: 17 Issue: 3 Pages: 218-233
  • DOI: 10.1111/j.1365-2443.2012.01583.x
  • Peer Reviewed
• [Journal Article] Heterochromatin protein 1 homologue Swi6 acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly (2012)
  • Author(s): Hayashi, A., Ishida, M., Kawaguchi, R., Urano, T., Murakami, Y., Nakayama, J-I.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: (印刷中) Issue: 16 Pages: 6159-64
  • DOI: 10.1073/pnas.1116972109
  • Peer Reviewed
• [Journal Article] Dynamic regulation of heterochromatin via phosphorylation of HP1-family proteins (2010)
  • Author(s): Shimada A. and Murakami Y.
  • Journal Title: Epigenetics Volume: 5 Pages: 30-33
• [Journal Article] Multiple Functions of S-phase Checkpoint Mediator. (2010)
  • Author(s): Tanaka, K
  • Journal Title: Biosci. Biotechnol. Biosci. Volume: 74 Pages: 2367-2373
  • NAID: 10027727602
  • Peer Reviewed
• [Journal Article] Dynamic regulation of heterochromatin via phosphorylation of HP1-family proteins. (2010)
  • Author(s): Shimada A
  • Journal Title: Epigenetics 5 Pages: 30-33
  • Peer Reviewed
• [Journal Article] Phosphorylation of Swi6/HP1 regulates transcriptional genesilencing at heterochromatin (2009)
  • Author(s): Shimada A., Dohke K., Sadaie M., Shinmyozu, K., Nakayama, J-I., Urano, T. and Murakami, Y
  • Journal Title: Genes & Dev Volume: 23 Pages: 18-23
• [Journal Article] Reconstitution of Arabidopsis thaliana SUMO pathways in E.coli : functional evaluation of SUMO machinery proteins and mapping of SUMOylation sites by mass spectrometry. (2009)
  • Author(s): Okada S
  • Journal Title: Plant Cell Physiol. 50 Pages: 1049-1061
  • Peer Reviewed
• [Presentation] 分裂酵母HP1ホモログSwi6の核酸結合機能 (2012)
  • Author(s): 山辺史貴、石田真由美、中山潤一、田中克典
  • Organizer: 第29回染色体ワークショップ
  • Place of Presentation: ホテルニュー水戸屋(仙台市)
  • Year and Date: 2012-01-26
• [Presentation] 分裂酵母新規蛋白質Dsh1は核膜近傍にsiRNA合成場を形成する (2012)
  • Author(s): 川上慶、林亜紀、中山潤一、小布施力史、村上洋太
  • Organizer: 第29回染色体ワークショップ
  • Place of Presentation: ホテルニュー水戸屋(仙台市)
  • Year and Date: 2012-01-26
• [Presentation] Non-coding RNA binds to heterochromatin via DNA : RNA hybrid to mediate RNAi-directed heterochromatin formation (2011)
  • Author(s): 村上洋太、高畑信也、梶谷卓也、鈴木詔大、千田香織、森美由紀
  • Organizer: 第34回日本分子生物学会年会シンポジウム
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-16
• [Presentation] Transcriptional Machinery including RNA polymerase II regulates transcriptional and post-transcriptional gene silencing at heterochromatin (2011)
  • Author(s): 村上洋太、高畑信也、梶谷卓也、鈴木詔大、千田香織、森美由紀
  • Organizer: 第34回日本分子生物学会年会シンポジウム「Mobile elements and its biological functions」
  • Place of Presentation: パシフィコ横浜(横浜市)(招待講演)
  • Year and Date: 2011-12-16
• [Presentation] Phosphorylations of RNAPII-CTD have two essential roles for centromeric silencing (2011)
  • Author(s): Kajitani, T., Hermand, D., Ishii, K., Kimura, H., Murakami, Y.
  • Organizer: Cell : Symposium : Epigenetics and the Inheritance o Acquired States
  • Place of Presentation: The Westin Boston Waterfront (Boston, USA)
  • Year and Date: 2011-10-31
• [Presentation] RNAPII-CTDのリン酸化はセントロメアの遺伝子発現抑制に必須の二つの機能をもつ (2011)
  • Author(s): 梶谷卓也、Damien Hermand, 石井浩二郎、木村宏、村上洋太
  • Organizer: 第10回核ダイナミクス研究会
  • Place of Presentation: 北広島クラッセホテル(北広島市)
  • Year and Date: 2011-10-27
• [Presentation] 分裂酵母新規蛋白質Dsh1は核膜近傍にsiRNA合成場を形成する (2011)
  • Author(s): 川上慶、林亜紀、中山潤一、小布施力史、村上洋太
  • Organizer: 第10回核ダイナミクス研究会
  • Place of Presentation: 北広島クラッセホテル(北広島市)
  • Year and Date: 2011-10-27
• [Presentation] Non-coding RNA binds to heterochromatin via DNA : RNA hybrid to mediate RNAi-directed heterochromatin formation (2011)
  • Author(s): 村上洋太
  • Organizer: Sweden-Japan Joint Colloquium, Epigenetics
  • Place of Presentation: ストックホルム(スウェーデン)
  • Year and Date: 2011-09-07
• [Presentation] Dsh1 assembles RNAi machinery and forms siRNA generation factory at Nuclear Periphery in fission yeast (2011)
  • Author(s): 村上洋太
  • Organizer: 第63回日本細胞生物学会大会シンポジウム
  • Place of Presentation: 北海道大学
  • Year and Date: 2011-06-27
• [Presentation] yeast (2011)
  • Author(s): 村上洋太
  • Organizer: 第63回日本細胞生物学会大会シンポジウム
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-06-27
• [Presentation] Non-coding RNA binds to heterochromatin via DNA : RNA hybrid to mediate RNAi-directed heterochromatin formation (2011)
  • Author(s): 村上洋太
  • Organizer: International Symposium, Physicochemical Field for Genetic Activities
  • Place of Presentation: 淡路夢舞台(淡路市)
  • Year and Date: 2011-01-25
• [Presentation] Non-coding RNA binds to heterochromatin via DNA : RNA hybrid to mediate RNAi-directed heterochromatin formation (2011)
  • Author(s): Yota Murakami
  • Organizer: International Symposium, Physicochemical Field for Genetic Activities
  • Place of Presentation: 兵庫県・淡路市
  • Year and Date: 2011-01-25
• [Presentation] Non-coding RNA binds to heterochromatin via DNA : RNA hybrid to mediate RNAi-directed heterochromatin formation (2010)
  • Author(s): 村上洋太、仲間美奈、川上慶、梶谷卓也、浦野健
  • Organizer: 第33回日本分子生物学会年会ワークショップ
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2010-12-08
• [Presentation] Non-coding RNA binds to heterochromatin via DNA : RNA hybrid to mediate RNAi-directed heterochromatin formation (2010)
  • Author(s): 村上洋太、仲間美奈、川上慶、梶谷卓也、浦野健
  • Organizer: 第33回日本分子生物学会年会ワークショップ「DNA・RNA・タンパク質のインタープレイがおりなす遺伝情報発現」
  • Place of Presentation: 兵庫県・神戸市
  • Year and Date: 2010-12-08
• [Presentation] RNAi依存性ヘテロクロマチンにおけるnon-coding RNAの動態と機能制御 (2010)
  • Author(s): 村上洋太
  • Organizer: 日本遺伝学会大会第82回大会シンポジウム
  • Place of Presentation: 北海道大学
  • Year and Date: 2010-09-20
• [Presentation] RNAi依存性ヘテロクロマチンにおけるnon-coding RNAの動態と機能制御 (2010)
  • Author(s): 村上洋太
  • Organizer: 日本遺伝学会大会第82回大会シンポジウム「多様な生命現象におけるエピゲノム制御」
  • Place of Presentation: 北海道、札幌市
  • Year and Date: 2010-09-20
• [Presentation] 分裂酵母HP1ホモログSwi6のSUMO化修飾の意義の解明 (2010)
  • Author(s): 山邊史貴、石田真由美、中山潤一、田中克典
  • Organizer: 第43回酵母遺伝学フォーラム
  • Place of Presentation: 奈良市
  • Year and Date: 2010-09-10
• [Presentation] Non-coding RNA binds to heterochromatin via DNA : RNA hybrid to mediate RNAi-directed heterochromatin formation (2010)
  • Author(s): Yota Murakami
  • Organizer: Sweden-Japan Joint Colloquium, Epigenetics
  • Place of Presentation: Stochholm, Sweden
  • Year and Date: 2010-09-07
• [Presentation] 分裂酵母HP1ホモログSwi6のSUMO化修飾の意義の解明 (2010)
  • Author(s): 山邊史貴、石田真由美、中山潤一、田中克典
  • Organizer: 第9回核ダイナミクス研究会
  • Place of Presentation: 静岡県伊豆市
  • Year and Date: 2010-05-29
• [Presentation] ヘテロクロマチンダイナミクスにおけるnon-coding RNA (2010)
  • Author(s): 村上洋太
  • Organizer: 第9回核ダイナミクス研究会
  • Place of Presentation: 静岡県伊豆市
  • Year and Date: 2010-05-29
• [Presentation] 分裂酵母MCM-BPタンパク質の機能解析 (2010)
  • Author(s): 田中克典
  • Organizer: 第27回染色体ワークショップ
  • Place of Presentation: 三島
• [Presentation] Dynamic Regulation of heterochromatin sturucture/function in the cell cycle (2009)
  • Author(s): 村上洋太
  • Organizer: 第32回日本分子生物学会年会ワークショップ「染色体倍加装置のダイナミクス」
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-12-10
• [Presentation] Dynamic Regulation of Heterochrimatin (2009)
  • Author(s): 村上洋太
  • Organizer: The fifth International Fission Yeast Meeting
  • Place of Presentation: 国立オリンピック記念青少年総合センター(東京都)
  • Year and Date: 2009-10-28
• [Presentation] トランスポゼース由来の分裂酵母CENP-Bホモログはキネトコアとヘテロクロマチンの境界で機能する (2009)
  • Author(s): 村上洋太
  • Organizer: 第82回日本生化学会大会シンポジウム
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2009-10-24
• [Presentation] Dynamic Regulation of Heterochrimatin (2009)
  • Author(s): 村上洋太
  • Organizer: 第24回内藤カンファレンス「Nuclear Dynamics and RNA [II]」
  • Place of Presentation: ガトーキングダム札幌(札幌市)
  • Year and Date: 2009-06-22
• [Presentation] Dynamic Regulation of Heterochrimatin (2009)
  • Author(s): 村上洋太
  • Organizer: 第24回 内藤カンファレンス"Nuclear Dynamics and RNA[II]
  • Place of Presentation: 北海道 札幌市
• [Presentation] トランスポゼース由来の分裂酵母CENP-Bホモログはキネトコアとヘテロクロマチンの境界で機能する (2009)
  • Author(s): 村上洋太
  • Organizer: 第82回日本生化学会大会 シンポジウム「トランスポゾンとの共生が織りなすゲノムシステムのダイナミクス」
  • Place of Presentation: 兵庫県 神戸市
• [Presentation] Dynamic Regulation of Heterochrimatin (2009)
  • Author(s): 村上洋太
  • Organizer: The fifth International Fission Yeast Meeting
  • Place of Presentation: 東京都
• [Presentation] Dynamic Regulation of heterochromatin sturucture/function in the cell cycle (2009)
  • Author(s): 村上洋太
  • Organizer: 第32回日本分子生物学会年会ワークショップ「染色体倍加装置のダイナミクス」
  • Place of Presentation: 神奈川県 横浜市
• [Presentation] 分裂酵母Mrc1のDNA複製フォークへの結合様式の解明 (2009)
  • Author(s): 坂井俊介
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] THE MRC1 LOADING ONTO THE REPLICATION FORK IN FISSION YEAST (2009)
  • Author(s): Katsunori Tanaka
  • Organizer: The 5th International Fission Yeast meeting Pombe2009
  • Place of Presentation: Tokyo, Japan
• [Remarks]
  • URL: http://barato.sci.hokudai.ac.jp/~bo/