single-molecule tracking elucidation of signal transduction by the molecular nano-complexes in the plasma membrane
| Project/Area Number |
21247022
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Kyoto University |
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Principal Investigator |
KUSUMI Akihiro 京都大学, 物質-細胞統合システム拠点, 教授 (50169992)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥46,930,000 (Direct Cost: ¥36,100,000、Indirect Cost: ¥10,830,000)
Fiscal Year 2011: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2010: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2009: ¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
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| Keywords | 1分子追跡 / 細胞膜 / ナノ集合体 / 情報変換 / システム / GPIアンカー型受容体 |
|---|
| Research Abstract |
The time resolution of single-molecule fluorescent imaging in the live cell plasma membrane has been improved to 0. 3ms, without losing the single-molecule localization accuracy of 30nm. This method was applied to study the signal transduction mechanism of the raft-associated molecule CD59, and the revealed the following mechanism. In resting cells, CD59trans-convert among monomers, homo-dimer rafts, and homo-tetramer rafts with lifetimes of 100-200ms. Upon ligation, CD59formed stable oligomer rafts, which triggered intracellular pulse-type signal responses.
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Report
(4 results)
Research Products
(78 results)
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[Journal Article] Integrated and diffusion-based micro-injectors for open access cell assays2011
Author(s)
X.Li, L.Liu, L.Wang, K.Kamei, Q.Yuan, F.Zhang, J.Shi, A.Kusumi, M.Xie, Z.Zhao, Y.Chen
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Journal Title
Lab Chip
Volume: 11
Issue: 15
Pages: 2612-2617
DOI
Related Report
Peer Reviewed
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[Journal Article] Equal contribution, Membrane molecules mobile even after chemical fixation2010
Author(s)
K. A. K. Tanaka, K. G. N. Suzuki, Y. M. Shirai, S. T. Shibutani, M. S. H. Miyahara, H. Tsuboi, M. Yahara, A. Yoshimura, S. Mayor, T. K. Fujiwara, A. Kusumi
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Journal Title
Nature Methods
Volume: Vol.7
Issue: 11
Pages: 865-866
DOI
Related Report
Peer Reviewed
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[Journal Article] Membrane molecules mobile even after chemical fixation2010
Author(s)
K.A.K.Tanaka, K.G.N.Suzuki, Y.M.Shirai, S.T.Shibutani, M.S.H.Miyahara, H.Tsuboi, M.Yahara, A.Yoshimura, S.Mayor, T.K.Fujiwara, A.Kusumi, Equal contribution
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Journal Title
Nature Methods
Volume: 7
Pages: 865-866
NAID
Related Report
Peer Reviewed
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[Presentation] Single-molecule tracking of PKC received and transferred by diffusing small antennas of signal-induced diacylglycerol2010
Author(s)
M.Xie, M.Ishibashi, I.Koyama-Honda, R.S.Kasai, A.Shibata, C.Nakada, T.K.Fujiwara, N.Saito, Y.Mori, L.Liu, D.W.Pang, Y.Chen, A.Kusumi
Organizer
第48回日本生物物理学会年会
Place of Presentation
仙台(招待講演)
Year and Date
2010-09-22
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