Mechanism and function of endocytosis in sucrose accumulation of plant cells

• Project/Area Number: 21248002
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: The University of Tokyo
• Principal Investigator: TSUTSUMI Nobuhiro 東京大学, 大学院・農学生命科学研究科, 教授 (00202185)
• Co-Investigator(Renkei-kenkyūsha): NAKAZONO Mikio 東京大学, 大学院・農学生命科学研究科, 教授 (70282697) ; ARIMURA Shin-ichi 東京大学, 大学院・農学生命科学研究科, 准教授 (00396938)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥43,290,000 (Direct Cost: ¥33,300,000、Indirect Cost: ¥9,990,000); Fiscal Year 2011: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000); Fiscal Year 2010: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000); Fiscal Year 2009: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
• Keywords: DRP1 / DRP2A / DRP2Bエンドサイトーシス / クラスリン / ダイナミン様タンパク質 / 全反射蛍光顕微鏡 / 小胞輸送 / 配偶体致死 / ダイナミン / DRP2 / エンドサイトーシス

Research Abstract

Endocytosis performs a wide range of functions in animals and plants. Clathrin-coated vesicle (CCV) formation is an initial step of endocytosis, and in animal cells is largely achieved by dynamins. However, little is known of its molecular mechanisms in plant cells. To identify dynamin-related proteins (DRPs) involved in endocytic CCV formation in plant cells, we compared the behaviors of two structurally different Arabidopsis DRPs, DRP2B and DRP1A. DRP2B and DRP1A colocalized and assembled/disassembled together at the plasma membrane in Arabidopsis cells. DRP2B and DRP1A participate together in endocytic CCV formation in Arabidopsis cells despite the difference of their molecular properties.

Research Products

• [Journal Article] A membrane trafficking pathway regulated by the plant-specific RAB GTPase ARA6 (2011)
  • Author(s): Ebine K, Fujimoto M, Okatani Y, Nishiyama T, Goh T, Ito E, Dainobu T, Nishitani A, Uemura T, Sato M, Thordal-Christensen H, Tsutsumi N, Nakano A, Ueda T
  • Journal Title: Nature Cell Biol. Volume: 13 Pages: 853-853
• [Journal Article] The rice mitochondrial iron transporter is essential for plant growth (2011)
  • Author(s): Bashir K, Ishimaru Y, Shimo H, Nagasaka S, Fujimoto M, Takanashi H, Tsutsumi N, An G, Nakanishi H, Nishizawa N
  • Journal Title: Nature Comm. Volume: 2 Pages: 322-322
• [Journal Article] Distinct gene expression profiles in egg and synergid cells of rice as revealed by cell type-specific microarrays (2011)
  • Author(s): Ohnishi T, Takanashi H, Mogi M, Takahashi H, Kikuchi S, Yano K, Okamoto T, Fujita M, Kurata N, Tsutsumi N
  • Journal Title: Plant Physiol. Volume: 155 Pages: 881-881
• [Journal Article] Identification of genes expressed in maize root cortical cells during lysigenous aerenchyma formation using laser microdissection and microarray analyses (2011)
  • Author(s): Rajhi I, Yamauchi T, Takahashi H, Nishiuchi S, Shiono K, Watanabe R, Mliki A, Nagamura Y, Tsutsumi N, Nishizawa NK, Nakazono M
  • Journal Title: New Phytol. Volume: 190 Pages: 351-368
• [Journal Article] Peroxisomes Are Involved in Biotin Biosynthesis in Aspergillus and Arabidopsis (2011)
  • Author(s): Tanabe Y., Maruyama J., Yeunaoka S.,Yahagi D., Matsuo I., Tsutsumi N., Kitamoto K.
  • Journal Title: JOURNAL OF BIOLOGICAL CHEMISTRY Volume: 286 Issue: 35 Pages: 30455-30461
  • DOI: 10.1074/jbc.m111.247338
  • Peer Reviewed
• [Journal Article] Cell division and cell elongation in the coleoptile of rice alcohol dehydrogenase 1-deficient mutant are reduced under complete submergence (2011)
  • Author(s): Takahashi H., Saika H., Matsumura H, Nagamura Y., Tsutsumi N., Nishizawa N., Nakazono M.
  • Journal Title: ANNALS OF BOTANY Volume: 108 Issue: 2 Pages: 253-261
  • DOI: 10.1093/aob/mcr137
  • Peer Reviewed
• [Journal Article] A membrane trafficking pathway regulated by the plant-specific RAB GTPase ARA6 (2011)
  • Author(s): Ebine K, Fujimoto M, Okatani Y, Nishiyama T, Gob T, Ito E, Dainobu T, Nishitani A, Uemura T, Sato MH, Thordal-Christensen H, Tsutsumi N, Nakano A, Ueda T
  • Journal Title: Nature Gell Biology Volume: 13 Issue: 7 Pages: 853-859
  • DOI: 10.1038/ncb2270
  • Peer Reviewed
• [Journal Article] The rice mitochondrial iron transporter is essential for plant growth (2011)
  • Author(s): Bashir K, Ishimaru Y, Shimo H, Nagasaka S, Fujimoto M, Takanashi H, Tsutsumi N, An G, Nakanishi H, Nishizawa NK
  • Journal Title: Nature Communications Volume: 2 Issue: 1 Pages: 322-322
  • DOI: 10.1038/ncomms1326
  • Peer Reviewed
• [Journal Article] MIRO1 influences the morphology and intracellular distribution of mitochondria during embryonic cell division in Arabidopsis (2011)
  • Author(s): Yamaoka S., Nakajima M., Fujimoto M., Tsutsumi N.
  • Journal Title: PLANT CELL REPORTS Volume: 30 Issue: 2 Pages: 239-244
  • DOI: 10.1007/s00299-010-0926-5
  • Peer Reviewed
• [Journal Article] Distinct gene expression profiles in egg and synergid cells of rice as revealed by cell type-specific microarrays (2011)
  • Author(s): Ohnishi T., Takanashi H., Mogi M., Takahashi H., Kikuchi H., Yano K., Okamoto T., Fujita M., Kurata N. and Tsutsumi N.
  • Journal Title: Plant Physiol Volume: 155 Issue: 2 Pages: 881-891
  • DOI: 10.1104/pp.110.167502
  • Peer Reviewed
• [Journal Article] Identification of genes expressed in maize root cortical cells during lysigenous aerenchyma formation using laser microdissection and microarray analyses (2011)
  • Author(s): Rajhi I., Yamauchi T., Takahashi H., Nishiuchi S., Shiono K., Watanabe R., Mliki A., Nagamura Y., Tsutsumi N., Nishizawa N., Nakazono M.
  • Journal Title: NEW PHYTOLOGIST Volume: 190 Issue: 2 Pages: 351-368
  • DOI: 10.1111/j.1469-8137.2010.03535.x
  • Peer Reviewed
• [Journal Article] Comprehensive network analysis of anther-expressed genes in rice by the combination of 33 laser microdissection and 143 spatiotemporal microarrays. (2011)
  • Author(s): Aya, et al.
  • Journal Title: PLoS One Volume: 6 Issue: 10 Pages: e26162-e26162
  • DOI: 10.1371/journal.pone.0026162
  • Peer Reviewed
• [Journal Article] A membrane trafficking pathway regulated by the plant-specific RAB GTP ase ARA6. (2011)
  • Author(s): Ebine, K.
  • Journal Title: Nature Cell Biol. Volume: (印刷中)(掲載確定)
  • Peer Reviewed
• [Journal Article] Different status of the gene for ribosomal protein S16 in the chloroplast genome during evolution of the genus Arabidopsis and closely related species (2010)
  • Author(s): Roy S, Ueda M, Kadowaki K, Tsutsumi N
  • Journal Title: Genes Genet Syst. Volume: 85 Pages: 319-26
  • NAID: 10029515663
• [Journal Article] The FtsH protease heterocomplex in arabidopsis : dispensability of type-B protease activity for proper chloroplast development (2010)
  • Author(s): Zhang D, Kato Y, Zhang L, Fujimoto M, Tsutsumi N, Sodmergen, Sakamoto W
  • Journal Title: Plant Cell Volume: 22 Pages: 3710-25
• [Journal Article] Rice expression atlas in reproductive development (2010)
  • Author(s): Fujita M, Horiuchi Y, Ueda Y, Mizuta Y, Kubo T, Yano K, Yamaki S, Tsuda K, Nagata T, Niihama M, Kato H, Kikuchi S, Hamada K, Mochizuki T, Ishimizu T, Iwai H, Tsutsumi N, Kurata N
  • Journal Title: Plant Cell Physiol. Volume: 51 Pages: 2060-81
  • NAID: 10028056583
• [Journal Article] MIRO1 influences the morphology and intracellular distribution of mitochondria during embryonic cell division in Arabidopsis (2010)
  • Author(s): Yamaoka S, Nakajima M, Fujimoto M, Tsutsumi N
  • Journal Title: Plant Cell Rep. Volume: 30 Pages: 239-44
• [Journal Article] Fusion of mitochondria in tobacco suspension cultured cells is dependent on the cellular ATP level but not on actin polymerization (2010)
  • Author(s): Wakamatsu K, Fujimoto M, Nakazono M, Arimura S, Tsutsumi N
  • Journal Title: Plant Cell Rep. Volume: 29 Pages: 1139-45
• [Journal Article] Strigolactones negatively regulate mesocotyl elongation in rice during germination and growth in darkness (2010)
  • Author(s): Hu Z, Yan H, Yang J, Yamaguchi S, Maekawa M, Takamure I, Tsutsumi N, Kyozuka J, Nakazono M
  • Journal Title: Plant Cell Physiol. Volume: 51 Pages: 1136-42
  • NAID: 10027350667
• [Journal Article] Arabidopsis dynamin-related proteins DRP2B and DRP1A participate together in clathrin-coated vesicle formation during endocytosis (2010)
  • Author(s): Fujimoto M, Arimura S, Ueda T, Takanashi H, Hayashi Y, Nakano A, Tsutsumi N
  • Journal Title: Proc Natl Acad Sci USA Volume: 107 Pages: 6094-9
• [Journal Article] The involvement of a PPR protein of the P subfamily in partial RNA editing of an Arabidopsis mitochondrial transcript (2010)
  • Author(s): Doniwa Y, Ueda M, Ueta M, Wada A, Kadowaki K, Tsutsumi N
  • Journal Title: Gene Volume: 454 Pages: 39-46
• [Journal Article] Studies of mitochondrial morphology and DNA amount in the rice egg cell (2010)
  • Author(s): Takanashi H, Ohnishi T, Mogi M, Okamoto T, Arimura S, Tsutsumi N
  • Journal Title: Curr Genet. Volume: 56 Pages: 33-41
• [Journal Article] The FtsH protease heterocomplex in arabidopsis : dispensability of type-B protease activity for proper chloroplast development. (2010)
  • Author(s): Zhang, D.
  • Journal Title: Plant Cell Volume: 22 Pages: 3710-3725
  • Peer Reviewed
• [Journal Article] Fusion of mitochondria in tobacco suspension cultured cells in dependent on the cellular ATP level but not on actin polymerization. (2010)
  • Author(s): Wakamateu, K.
  • Journal Title: Plant Cell Rep. Volume: 29 Pages: 1139-1145
  • Peer Reviewed
• [Journal Article] Arabidopsis dynamin-related proteins DRP2B and DRP1A participate together in clathrin-coated vesicle formation during endocytosis (2010)
  • Author(s): Fujimoto, M.
  • Journal Title: Proc.Nat.Acad.Sci.USA 107 Pages: 6094-6099
  • Peer Reviewed
• [Journal Article] Studies of mitochondrial morphology and DNA amount in the rice egg cell. (2010)
  • Author(s): Takanashi, H.
  • Journal Title: Curr.Genet. 56 Pages: 33-41
  • Peer Reviewed
• [Journal Article] The involvement of a PPR protein of the P subfamily in partial RNA editing of an Arabidopsis mitochondrial transcript. (2010)
  • Author(s): Doniwa, Y.
  • Journal Title: Gene 454 Pages: 39-46
  • Peer Reviewed
• [Journal Article] Rice-specific mitochondrial iron-regulated gene (MIR) plays an important role in iron homeostasis (2009)
  • Author(s): Ishimaru Y, Bashir K, Fujimoto M, An G, Itai RN, Tsutsumi N, Nakanishi H, Nishizawa NK
  • Journal Title: Mol Plant. Volume: 2 Pages: 1059-66
• [Journal Article] Arabidopsis dynamin-related proteins DRP3A and DRP3B are functionally redundant in mitochondrial fission, but have distinct roles in peroxisomal fission (2009)
  • Author(s): Fujimoto M, Arimura S, Mano S, Kondo M, Saito C, Ueda T, Nakazono M, Nakano A, Nishimura M, Tsutsumi N
  • Journal Title: Plant J. Volume: 58 Pages: 388-400
• [Journal Article] Functional association of cell death suppressor, Arabidopsis Bax inhibitor-1, with fatty acid 2-hydroxylation through cytochrome b (2009)
  • Author(s): Nagano M, Ihara-Ohori Y, Imai H, Inada N, Fujimoto M, Tsutsumi N, Uchimiya H, Kawai-Yamada M
  • Journal Title: Plant J. Volume: 58 Pages: 122-34