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Epigenetics on transdifferentiation of ES cells toward TS cells

Research Project

Project/Area Number 21248039
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionThe University of Tokyo

Principal Investigator

TANAKA Satoshi  東京大学, 大学院・農学生命科学研究科, 准教授 (90242164)

Co-Investigator(Kenkyū-buntansha) OHGANE Jun  明治大学, 農学部, 講師 (50313078)
Co-Investigator(Renkei-kenkyūsha) SHIOTA Kunio  東京大学, 大学院・農学生命科学研究科, 教授 (80196352)
FURUE Miho  独立行政法人医薬基盤研究所, 生物資源研究部門, 研究員 (80257310)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥29,120,000 (Direct Cost: ¥22,400,000、Indirect Cost: ¥6,720,000)
Fiscal Year 2011: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2010: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Keywords胚性幹細胞 / 栄養膜細胞 / 細胞分化 / 分化転換 / エピジェネティクス
Research Abstract

To elucidate if mESCs pass through TSC-like and/or EpiSC-like state during the process of BMP4-induced transdifferentiation, we investigated nature of intermediate cell population in the transdifferentiation process. Immunohistochemistry revealed the presence of TSC-like cell colonies only in the BMP4-treated cultures. We then subcultured BMP4-treated mESCs under the conditions for maintenance of TSCs, and consequently obtained a cell line. DNA microarray analysis revealed that the gene expression profile of then new cell line is more similar to that of EpiSCs compared to mTSCs and mESCs. These results suggest that BMP4-treated mESCs may transdifferentiate to TCs through two independent pathways. One is the pathway through mTSC-like state and the other through mEpiSC-like state.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (4 results)

All 2011 2009

All Presentation (4 results)

  • [Presentation] Study on a BMP4-induced transdifferentiation process of mouse embryonic stem cells toward trophoblast cell fate. The 2^nd World Congress on Reproductive Biology2011

    • Author(s)
      Fuyuki Adachi, Kunio Shiota and Satoshi Tanaka
    • Place of Presentation
      Cairns, Australia
    • Year and Date
      2011-10-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Study on a BMP4-induced transdifferentiation process of mouse embryonic stem cells toward trophoblast cell fate2011

    • Author(s)
      Fuyuki Adachi, et al.
    • Organizer
      The 2^<nd> World Congress on Reproductive Biology
    • Place of Presentation
      Cairns, Australia(ポスター)
    • Year and Date
      2011-10-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] TS細胞をもちいたトロホブラスト研究-クローン胚のトロホブラストは異常か?2009

    • Author(s)
      田中智
    • Organizer
      第17回日本胎盤学会学術集会
    • Place of Presentation
      東京ミッドタウン(東京都)
    • Year and Date
      2009-10-16
    • Related Report
      2011 Final Research Report
  • [Presentation] TS細胞をもちいたトロホブラスト研究-クローン胚のトロホブラストは異常か?-2009

    • Author(s)
      田中智
    • Organizer
      第17回日本胎盤学会学術集会
    • Place of Presentation
      東京ミッドタウン(東京都)
    • Year and Date
      2009-10-16
    • Related Report
      2009 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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