Study of induction mechanisms of autophagy in innate immunity

• Project/Area Number: 21249004
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Tohoku University
• Principal Investigator: KURATA Shoichiro 東北大学, 大学院・薬学研究科, 教授 (90221944)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥46,670,000 (Direct Cost: ¥35,900,000、Indirect Cost: ¥10,770,000); Fiscal Year 2011: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000); Fiscal Year 2010: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000); Fiscal Year 2009: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
• Keywords: 自然免疫 / オートファジー / ショウジョウバエ / 抗菌ペプチド / PGRP

Research Abstract

PGRP-LE, a pathogen sensor in Drosophila, induces autophagy after recognition of bacteria such as Listeria in the cytoplasm. A Drosophila homologue of p62 (SQSTM1)encoding by Ref(2)P was identified as a down-stream regulator showing direct binding to PGRP-LE, that is crucial for PGRP-LE-dependent induction of autophagy and host survival after Listerial infection. Moreover, a functional domain of PGRP-LE required for autophagy induction was identified.

Research Products

• [Journal Article] Intracellular Recognition of Pathogens and Autophagy as an Innate Immune Host Defense (2011)
  • Author(s): Yano, T., and Kurata, S.
  • Journal Title: J. Biochem Volume: 150 Pages: 143-149
  • Peer Reviewed
• [Journal Article] A phytoceramide analog_stimulates the production of chemokines through CREB activation in human endothelial cells (2011)
  • Author(s): Sekiya, M., Ueda, K., Okazaki, K., Terashima, J., Katou, Y., Kikuchi, H., Kurata, S., Oshima, Y.
  • Journal Title: Int.Immunopharmacol. Volume: 11 Pages: 1497-1503
  • Peer Reviewed
• [Journal Article] Intracellular Recognition of Pathogens and Autophagy as an Innate Immune Host Defense (2011)
  • Author(s): Yano, T., Kurata, S
  • Journal Title: J.Biochem. Volume: 150 Pages: 143-149
  • Peer Reviewed
• [Journal Article] Cooperative regulation of the induction of the novel antibacterial listericin by PGRP-LE and the JAK-STAT pathway (2010)
  • Author(s): Goto, A., Yano, T., Terashima, J., Iwashita, S., Oshima, Y. and Kurata, S.
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 15731-15738
  • Peer Reviewed
• [Journal Article] Extracellular and intracellular pathogen recognition by Drosophila PGRP-LE and PGRP-LC (2010)
  • Author(s): Kurata, S.
  • Journal Title: Int. Immunol Volume: 22 Pages: 143-148
  • NAID: 10027391539
  • Peer Reviewed
• [Journal Article] Cooperative regulation of the induction of the novel antibacterial listericin by PGRP-LE and the JAK-STAT pathway (2010)
  • Author(s): Goto, A., Yano, T., Terashima, J., Iwashita, S., Oshima, Y., Kurata, S.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 15731-15738
  • Peer Reviewed
• [Journal Article] Extracellular and intracellular pathogen recognition by Drosophila PGRP-LE and PGRP-LC (2010)
  • Author(s): Kurata, S.
  • Journal Title: Int.Immunol. Volume: 42 Pages: 143-148
  • NAID: 10027391539
  • Peer Reviewed
• [Journal Article] ショウジョウバエ病原体センサーPGRP-LEによる炎症誘導とオートファジー誘導 (2010)
  • Author(s): 倉田祥一朗
  • Journal Title: 炎症と免疫 Volume: 18 Pages: 3-7
• [Journal Article] An unexpected twist for autophagy in Crohn's desease (2009)
  • Author(s): Yano, T., and Kurata, S.
  • Journal Title: Nature Immunol Volume: 10 Pages: 134-136
  • Peer Reviewed
• [Journal Article] An unexpected twist for autophagy in Crohn's desease. (2009)
  • Author(s): Yano, T., 倉田祥一朗
  • Journal Title: Nature Immunology 10 Pages: 134-136
  • Peer Reviewed
• [Journal Article] 昆虫の自然免疫機構を解き明かす (2009)
  • Author(s): 倉田祥一朗
  • Journal Title: 化学 64 Pages: 12-17
• [Journal Article] PGRPを介したオートファジー誘導機構 (2009)
  • Author(s): 倉田祥一朗
  • Journal Title: 感染炎症免疫 39 Pages: 66-69
• [Presentation] ショウジョウバエ自然免疫における病原細菌の認識と排除の分子機構 (2012)
  • Author(s): 倉田祥一朗
  • Organizer: 岩手医科大学第6回先端医療薬学研究センター講演会:薬と医・歯の連携へ～基礎から病気まで～
  • Place of Presentation: 岩手医科大学(招待講演)
  • Year and Date: 2012-03-16
• [Presentation] A receptor guanylyl cyclase mediates humoral and cellular responses in Drosophila immunity (2011)
  • Author(s): Kurata, S.
  • Organizer: 8^<th> International Congress of Comparative Physiology and Biochemistry
  • Place of Presentation: Nagoya
  • Year and Date: 2011-06-03
• [Presentation] Recognition of intracellular bacterial infections and induction of autophagy in Drosophila immunity (2010)
  • Author(s): Kurata, S.
  • Organizer: The 2010 Gordon Research Conference on Autophagy in Stress, Development & Disease
  • Place of Presentation: Lucca (Barga), Italy
  • Year and Date: 2010-04-28
• [Presentation] Induction of autophagy via intracellular innate immune recognition in Drosophila (2009)
  • Author(s): Kurata, S.
  • Organizer: 5th International Symposium on Autophagy
  • Place of Presentation: Otsu, Japan
• [Book] Fly immunity ; recognition of pathogens and induction of immune responses (Chapter 11) (2011)
  • Author(s): Kurata, S.
  • Publisher: Landes Bioscience and Springer Science+ Business Media
• [Book] Invertebrate Immunity (2010)
  • Author(s): Kenneth Soderhall (Ed.)
  • Publisher: Landes Bioscience and Springer Science+Business Media
• [Remarks]
  • URL: http://www.pharm.tohoku.ac.jp/~seimei/seimei_original.html
• [Patent(Industrial Property Rights)] センサーの分子濃度依存シグナル増幅を利用した検出方法 (2009)
  • Inventor(s): 倉田祥一朗、大島吉輝、矢野環
  • Industrial Property Rights Holder: 国立大学法人東北大学
  • Industrial Property Number: 2009-160130
  • Filing Date: 2009-07-06