Measles pathogenesis as studied using animal models

• Project/Area Number: 21249032
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Kyushu University
• Principal Investigator: YANAGI Yusuke 九州大学, 医学研究院, 教授 (40182365)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥46,280,000 (Direct Cost: ¥35,600,000、Indirect Cost: ¥10,680,000); Fiscal Year 2012: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000); Fiscal Year 2011: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000); Fiscal Year 2010: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000); Fiscal Year 2009: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
• Keywords: 麻疹ウイルス / 動物モデル / 受容体 / インターフェロン / 膜融合 / ウイルス / 感染症 / 微生物 / SLAM / 免疫抑制

Research Abstract

SLAM knockin mice well reproduced immunological abnormalities observed in human measles. However, measles virus only grew in type I interferon receptor knockout mice, since it could not effectively counteract type I interferons in mouse cells. To overcome this problem, transgenic mice were established in which type I interferons are suppressed in measles virus-infected cells. Furthermore, it was demonstrated using 10-day-old suckling hamsters that enhanced fusion activity of the measles virus fusionprotein plays an important role in measles virus spread in neuronal cells that lack efficient receptors.

Research Products

• [Journal Article] Mutant fusion proteins with enhanced fusion activity promote measles virus spread in human neuronal cells and brains of suckling hamsters (2013)
  • Author(s): Watanabe S. Shirogane Y. Suzuki SO, Ikegame S, Koga R, Yanagi Y
  • Journal Title: Journal of Virology Volume: 87(5) Issue: 5 Pages: 2648-2659
  • DOI: 10.1128/jvi.02632-12
  • Peer Reviewed
• [Journal Article] Cooperation between different RNA virus genomes produces a new phenotype (2012)
  • Author(s): Shirogane Y, Watanabe S, Yanagi Y
  • Journal Title: ature Communications Volume: 3 Issue: 1 Pages: 1235-1235
  • DOI: 10.1038/ncomms2252
  • Peer Reviewed
• [Journal Article] Expression of the Sendai (murine parainfluenza) virus C protein alleviates restriction of measles virus growth in mouse cells (2011)
  • Author(s): Iwasaki M, Yanagi Y
  • Journal Title: Proceedings of National Academy of Sciences of USA Volume: 108(37) Issue: 37 Pages: 15384-15389
  • DOI: 10.1073/pnas.1107382108
• [Journal Article] Measles Virus-Induced Immunosuppression in SLAM Knock-In Mice (2010)
  • Author(s): Koga R, Ohno S, Ikegame S, Yanagi Y
  • Journal Title: Journal of Virology Volume: 84(10) Issue: 10 Pages: 5360-5367
  • DOI: 10.1128/jvi.02525-09
  • NAID: 120005289495
• [Journal Article] Measles virus-induced immunosuppression in SLAM knock-in mice (2010)
  • Author(s): Koga R, et al.
  • Journal Title: Journal of Virology Volume: 84 Pages: 5360-5367
  • Peer Reviewed
• [Journal Article] Measles virus-induced immunosuppression in SLAM knock-in mice (2010)
  • Author(s): Koga R, et al.
  • Journal Title: Journal of Virology 84(印刷中)
  • Peer Reviewed
• [Presentation] Measles virus entry and fusion (2013)
  • Author(s): Yanagi Y
  • Organizer: The 2nd Meeting on RNA and Biofunctions-ASIA Study " RNABiofunctions and Viruses"
  • Place of Presentation: 福岡
  • Year and Date: 2013-01-10
• [Presentation] Enhanced fusion activity is critical for measles virus spread in the central nervous system (2013)
  • Author(s): Watanabe S, Shirogane Y, Suzuki SO, Ikegame S, Koga R, Yanagi Y
  • Organizer: The 2nd Meeting on RNA and Biofunctions-ASIA Study "RNA Biofunctions and Viruses"
  • Place of Presentation: 福岡
  • Year and Date: 2013-01-10
• [Presentation] Measles virus entry and fusion (2013)
  • Author(s): Yanagi Y
  • Organizer: The 2nd Meeting on RNA and Biofunctions-ASIA Study “RNA Biofunctions and Viruses”
  • Place of Presentation: 福岡
  • Invited
• [Presentation] Enhanced fusion activity is critical for measles virus spread in the central nervous system (2013)
  • Author(s): Watanabe S (Yanagi Y)
  • Organizer: The 2nd Meeting on RNA and Biofunctions-ASIA Study “RNA Biofunctions and Viruses”
  • Place of Presentation: 福岡
• [Presentation] 麻疹ウイルスの神経病原性はウイルスの膜融合能によって規定される (2012)
  • Author(s): 渡辺俊平、白銀勇太、鈴木諭、池亀聡、古賀律子、柳雄介
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2012-11-15
• [Presentation] Paramyxovirus entry and fusion (2012)
  • Author(s): Yanagi Y
  • Organizer: The 34th Naito Conference on "Infection, Immunity and theirControl for Health: Mucosal Barrier, Pathogen and Vaccine"
  • Place of Presentation: 札幌
  • Year and Date: 2012-10-17
• [Presentation] Paramyxovirus entry and fusion (2012)
  • Author(s): Yanagi Y
  • Organizer: The 34th Naito Conference on “Infection, Immunity and their Control for Health: Mucosal Barrier, Pathogen and Vaccine”
  • Place of Presentation: 札幌
  • Invited
• [Presentation] The Sendai virus C protein supports efficient growth of measles virus in mouse cell lines (2011)
  • Author(s): Iwasaki M, Yanagi Y
  • Organizer: 15th International Congress of Virology
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-13
• [Presentation] Novel mutations in the measles virus fusion protein that enhance its fusion activity (2011)
  • Author(s): Watanabe S, Shirogane Y, Ikegame S, Koga R, Nakashima M, Yanagi Y
  • Organizer: 15th International Congress of Virology
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-13
• [Presentation] The Sendai virus C protein supports efficient growth of measles virus in mouse cell lines (2011)
  • Author(s): Iwasaki M, Yanagi Y
  • Organizer: 15th International Congress of Virology
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2011-09-13
• [Presentation] 細胞融合能を促進させる、麻疹ウイルスF蛋白質の新規アミノ酸変異の同定 (2011)
  • Author(s): 渡辺俊平、白銀勇太、池亀聡、古賀律子、柳雄介
  • Organizer: 第48回日本ウイルス学会九州支部総会
  • Place of Presentation: 北九州
  • Year and Date: 2011-08-27
• [Presentation] 麻疹ウイルス-膜融合機構と新しい動物モデルの開発- (2011)
  • Author(s): 柳 雄介
  • Organizer: 京都大学ウイルス研究所学術講演会
  • Place of Presentation: 京都
  • Year and Date: 2011-07-05
• [Presentation] 麻疹ウイルス-膜融合機構と新しい動物モデルの開発- (2011)
  • Author(s): 柳雄介
  • Organizer: 京都大学ウイルス研究所学術講演会
  • Place of Presentation: 京都大学(招待講演)
  • Year and Date: 2011-07-05
• [Presentation] マウス神経細胞の麻疹ウイルス感染 (2010)
  • Author(s): 古賀 律子、池亀聡、大野真治、渡辺俊平、柳 雄介
  • Organizer: 第58回 日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-08
• [Presentation] センダイウイルスCタンパク質は麻疹ウイルスのマウス培養細胞での増殖を促進する (2010)
  • Author(s): 岩崎正治、池亀聡、白銀勇太、柳雄介
  • Organizer: 第58回 日本ウイルス学会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-08
• [Presentation] マウス神経細胞の麻疹ウイルス感染 (2010)
  • Author(s): 古賀律子, 他
  • Organizer: 第58回 日本ウイルス学会学術集会
  • Place of Presentation: あわぎんホール(徳島)
  • Year and Date: 2010-11-08
• [Presentation] Measles virus tropism and pathogenesis (2009)
  • Author(s): Yanagi Y
  • Organizer: The 4th Nagaski Symposium on tropical and emerging infectious diseases
  • Place of Presentation: Nagasaki, Japan
  • Year and Date: 2009-11-26
• [Presentation] 麻疹ウイルスによる免疫抑制機構の検討 (2009)
  • Author(s): 古賀 律子、大野真治、柳雄介
  • Organizer: 第57回 日本ウイルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-27
• [Presentation] 麻疹ウイルスによる免疫抑制機構の検討 (2009)
  • Author(s): 古賀律子, 他
  • Organizer: 第57回 日本ウイルス学会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-27
• [Presentation] Measles virus tropism and pathogenesis (2009)
  • Author(s): Yanagi Y
  • Organizer: The 4th Nagaski Symposium on tropical and emerging infectious diseases
  • Place of Presentation: 長崎
  • Year and Date: 2009-09-26
• [Remarks]
  • URL: http://www.med.kyushu-u.ac.jp/virus/index.html
• [Remarks] 九州大学大学院医学研究院ウイルス学ホームページ
  • URL: http://www.med.kyushu-u.ac.jp/virus/index.html
• [Remarks]
  • URL: http://www.med.kyushu-u.ac.jp/virus/index.html
• [Remarks]
  • URL: http://www.med.kyushu-u.ac.jp/virus/index.html
• [Remarks]
  • URL: http://www.med.kyushu-u.ac.jp/virus/index.html