Cellular and molecular basis of hippocampal presynaptic calcium store

• Project/Area Number: 21300146
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Hokkaido University
• Principal Investigator: KAMIYA Haruyuki 北海道大学, 大学院・医学研究科, 教授 (10194979)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2009: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
• Keywords: シナプス伝達 / カルシウムイオン / 海馬 / 神経伝達物質放出

Research Abstract

Subcellular localization of calcium releasing sites from presynaptic calcium store in hippocampal mossy fiber synapse was examined. Using high speed confocal microscope system, calcium imaging within mossy fiber terminals and axons was performed. Calcium indicator was efficiently introduced to single mossy fiber terminals and axons by electroporation through microelectrode placed on visually identified mossy fiber terminals.

Research Products

• [Journal Article] Heterosynaptic enhancement of the excitability of hippocampal mossy fibers by long-range spill-over of glutamate (2012)
  • Author(s): Uchida T., Fukuda S., Kamiya H.
  • Journal Title: Hippocampus Volume: 22(2) Issue: 2 Pages: 222-229
  • DOI: 10.1002/hipo.20885
  • Peer Reviewed
• [Journal Article] Assessing the roles of presynaptic ryanodine receptors and adenosine receptors in caffeine-induced enhancement of hippocampal mossy fiber transmission (2011)
  • Author(s): Sato I., Kamiya H.
  • Journal Title: Neuroscience Research Volume: 71(3) Issue: 2 Pages: 183-187
  • DOI: 10.1016/j.neures.2011.07.001
  • Peer Reviewed
• [Journal Article] Heterosynaptic enhancement of the excitability of hippocampal mossy fibers by long-range spill-over of glutamate (2010)
  • Author(s): Uchida, T., et al
  • Journal Title: Hippocampus Volume: Nov3(Epub)
  • Peer Reviewed
• [Journal Article] receptor GluN2B (GluR epsilon 2/NR2B)subunit is crucial for channel function, postsynaptic macromolecular organization, and actin cytoskeleton at hippocampal CA3 synapses (2009)
  • Author(s): Akashi K., Kakizaki T., Kamiya H., Fukaya M., Yamasaki M., Abe M. NatsumeR., Watanabe M., Sakimura K. NMDA
  • Journal Title: Journal of Neuroscience, 29(35) Volume: 29 Issue: 35 Pages: 10869-10882
  • DOI: 10.1523/jneurosci.5531-08.2009
  • Peer Reviewed
• [Journal Article] NMDA receptor GluN2B(GluRε2/NR2B)subunit is crucial for channel function, postsynaptic macromolecular organization, and actin cytoskeleton at hippocampal CA3 synapses (2009)
  • Author(s): Akashi, K., et al.
  • Journal Title: Journal of Neuroscience 29 Pages: 10869-10882
  • Peer Reviewed
• [Presentation] 光反応性ブロッカーを用いたグルタミン酸受容体動態のシナプス「その場」解析 (2012)
  • Author(s): 神谷温之
  • Organizer: 第89回日本生理学会大会(シンポジウム)
  • Place of Presentation: 信州大学(長野)
  • Year and Date: 2012-03-31
• [Presentation] 光反応性ブロッカーを用いたグルタミン酸受容体動態のシナプス「その場」解析 (2012)
  • Author(s): 神谷温之
  • Organizer: 第89回日本生理学会
  • Place of Presentation: 信州大学(松本)(招待講演)
  • Year and Date: 2012-03-31
• [Presentation] Photoinactivation analysis of postsynaptic AMPA receptor dynamics during hippocampal long-term potentiation (2011)
  • Author(s): Kamiya H.
  • Organizer: Neuroscience 2011
  • Place of Presentation: Washington DC (USA)
  • Year and Date: 2011-11-16
• [Presentation] Photoinactivation analysis of postsynaptic AMPA receptor dynamics during hippocampal long-term potentiation (2011)
  • Author(s): Kamiya, H.
  • Organizer: Neuroscience 2011
  • Place of Presentation: Washington Convention Center (USA)
  • Year and Date: 2011-11-16
• [Presentation] 単一神経終末エレクトロポレーション法による海馬苔状線維軸索と神経終末の蛍光観察 (2011)
  • Author(s): 神谷温之
  • Organizer: 第34回日本神経科学学会大会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-09-17
• [Presentation] 単一神経終末エレクトロポレーション法による海馬苔状線維軸索と神経終末の蛍光観察 (2011)
  • Author(s): 神谷温之
  • Organizer: 第34回日本神経科学学会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-09-17
• [Presentation] 光不活化法による海馬CA1野シナプスAMPA 受容体動態の長期計測 (2011)
  • Author(s): 神谷温之
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: パシフィコ横浜(神奈川)(誌上開催)
  • Year and Date: 2011-03-28
• [Presentation] 光不活化法による海馬CA1野シナプスAMPA受容体動態の長期計測 (2011)
  • Author(s): 神谷温之
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: パシフィコ横浜(神奈川)(誌上開催)
  • Year and Date: 2011-03-28
• [Presentation] Photochemical inactivation of postsynaptic AMPA receptors in hippocampal slices (2010)
  • Author(s): Kamiya H.
  • Organizer: Neuroscience 2010
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2010-11-15
• [Presentation] Photochemical inactivation of postsynaptic AMPA receptors in hippocampal slices (2010)
  • Author(s): Kamiya, H.
  • Organizer: Neuroscience 2010
  • Place of Presentation: USA
  • Year and Date: 2010-11-15
• [Presentation] 海馬スライスでのAMPA受容体の不可逆的な光不活化 (2010)
  • Author(s): 神谷温之
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市民文化ホール(岩手)
  • Year and Date: 2010-05-20
• [Presentation] Bumetanide-sensitive and-insensitive actions of GABA_A receptors on the excitability of the hippocampal mossy fibers (2009)
  • Author(s): Kamiya H.
  • Organizer: 36th International Congress of Physiological Sciences
  • Place of Presentation: 京都国際会館(京都)
  • Year and Date: 2009-07-29
• [Presentation] Bumetanide-sensitive and -insensitive actions of GABA_A receptors on the excitability of the hippocampal mossy fibers (2009)
  • Author(s): Kamiya, H.
  • Organizer: 36th International Congress of Physiological Sciences
  • Place of Presentation: 京都国際会館(京都)
  • Year and Date: 2009-07-29