Study of Bisphenol A effect on neonatal mouse using genetically modified mouse

• Project/Area Number: 21310047
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Osaka University
• Principal Investigator: WATANABE Hajime 大阪大学, 工学研究科, 教授 (80212322)
• Co-Investigator(Kenkyū-buntansha): 井口 泰泉 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (90128588)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2009: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
• Keywords: マウス / エピジェネティクス / ビスフェノール / ビスフェノールA / エストロゲン / 新生児影響 / 化学物質影響

Research Abstract

It has been known that neonatal exposure of Bisphenol A cause adverse effects on mice. Especially effect on nervous stem is one of the great concerns. In this study, I made transgenic mice expressing a marker gene under the control of regulatory region of estrogen receptor alpha and examined the effect of Bisphenol A. In addition, I also examined the effect of Bisphenol A from epigenetic point of view by immunoprecipitation of methylated genome DNA.

Research Products

• [Journal Article] Development of an RNA interference method in the cladoceran crustacean Daphnia magna (2012)
  • Author(s): Kato, Y., Shiga, Y., Kobayashi, K., Tokishita, SI., Yamagata, H., Iguchi, T. & Watanabe, H.
  • Journal Title: Dev Genes Evol Volume: 20 Pages: 337-345
  • Peer Reviewed
• [Journal Article] Involvement of gonadotropins in the induction of hypertrophy-hyperplasia in the interstitial tissues of ovaries in neonatally diethylstilbestrol-treated mice (2012)
  • Author(s): Kakuta, H., Tanaka, M., Chambon, P., Watanabe, H., Iguchi, T., & Sato, T
  • Journal Title: Reproductive Toxicology Volume: 33 Pages: 35-44
  • Peer Reviewed
• [Journal Article] Wnt family genes and their modulation in the ovary-independent and persistent vaginal epithelial cell proliferation and keratinization induced by neonatal diethylstilbestrol exposure in mice (2012)
  • Author(s): Nakamura, T., Miyagawa, S., Katsu, Y., Watanabe, H., Mizutani, T., Sato, T., Morohashi, KI., Takeuchi, T., Iguchi, T., & Ohta, Y
  • Journal Title: Toxycology Volume: 296 Pages: 13-19
  • Peer Reviewed
• [Journal Article] Environmental Sex Determination in the Branchiopod Crustacean Daphinia magna : Deep Conservation of a Doublesex Gene in the Sex-Determining Pathway (2011)
  • Author(s): Kato, Y., Kobayashi, K., Watanabe, H., & Iguchi, T.
  • Journal Title: Plos Genet Volume: 7
  • Peer Reviewed
• [Journal Article] Development of an RNA interference method in the cladoceran crustacean Daphnia magna. (2011)
  • Author(s): Kato Y, Shiga Y, Kobayashi K, Tokishita SI, Yamagata H, Iguchi T, Watanabe H.
  • Journal Title: Dev Genes Evol. Volume: 220 Pages: 337-345
  • Peer Reviewed
• [Journal Article] Morphological changes in Daphnia galeata induced by acrustacean terpenoid hormone and its analog. (2011)
  • Author(s): Oda S, Kato Y, Watanabe H, Tatarazako N, Iguchi T.
  • Journal Title: Environ Toxicol Chem. Volume: 30 Pages: 232-238
  • Peer Reviewed
• [Journal Article] Environmental Sex Determination in the Branchiopod Crustacean Daphnia magna : Deep Conservation of a Doublesex Gene in the Sex-Determining Pathway (2011)
  • Author(s): Kato Y, Kobayashi K, Watanabe H, Iguchi T.
  • Journal Title: PLoS Genetics. Volume: 7
  • NAID: 120004837638
  • Peer Reviewed
• [Journal Article] Introduction of foreign DNA into the water flea, Daphnia magna, by electroporation (2010)
  • Author(s): Kato, Y., Kobayashi, K., Watanabe, H. & Iguchi, T.
  • Journal Title: Ecotoxicology Volume: 19 Pages: 589-592
  • Peer Reviewed
• [Journal Article] Sequence divergence and expression of a transformer gene in the branchiopod crustacean, Daphnia magna (2010)
  • Author(s): Kato, Y., Kobayashi, K., Oda, S., Tatarazako, N., Watanabe, H. & Iguchi, T.
  • Journal Title: Genomics Volume: 95 Pages: 160-165
  • Peer Reviewed
• [Journal Article] Differential involvement of insulin-like growth factor-I and estrogen on prolactin cells in the mouse anterior pituitary (2010)
  • Author(s): Hikake, T., Hayashi, S., Chambon, P., Watanabe, H., Iguchi, T. & Sato, T.
  • Journal Title: Exp Biol Med(Maywood) Volume: 235 Pages: 974-980
  • Peer Reviewed
• [Journal Article] Introduction of foreign DNA into the water flea, Daphnia magna, by electroporation. (2010)
  • Author(s): Kato Y, Kobayashi K, Watanabe H, Iguchi T.
  • Journal Title: Ecotoxicology Volume: 19 Pages: 589-92
  • Peer Reviewed
• [Journal Article] Sequence divergence and expression of a transformer gene in the branchiopod crustacean, Daphnia magna. (2010)
  • Author(s): Kato Y, Kobayashi K, Oda S, Tatarazako N, Watanabe H, Iguchi T.
  • Journal Title: Genomics. Volume: 95 Pages: 160-5
  • Peer Reviewed
• [Journal Article] Introduction of foreign DNA into the water flea, Daphnia magna, by electroporation. (2010)
  • Author(s): Kato Y, Kobayashi K, Watanabe H, Iguchi T.
  • Journal Title: Ecotoxicology 19 Pages: 589-92
  • Peer Reviewed
• [Journal Article] Sequence divergence and expression of a transformer gene in the branc hiopod crustacean, Daphnia magna. (2010)
  • Author(s): Kato Y, Kobayashi K, Oda S, Tatarazako N, Watanabe H, Iguchi T.
  • Journal Title: Genomics. 95
  • Peer Reviewed
• [Journal Article] Involvement of Estrogen Receptor beta in the Induction of Polyovular Follicles in Mouse Ovaries Exposed Neonatally to Diethylstilbestrol (2009)
  • Author(s): Kirigaya, A., Kim, H., Hayashi, S., Chambon, P., Watanabe, H., Iguchi, T. & Sato, T.
  • Journal Title: Zoological Science Volume: 26 Pages: 704-712
  • Peer Reviewed
• [Journal Article] Effects of diethylstilbestrol on programmed oocyte death and induction of polyovular follicles in neonatal mouse ovaries (2009)
  • Author(s): Kim, H., Nakajima, T., Hayashi, S., Chambon, P., Watanabe, H., Iguchi, T. & Sato, T.
  • Journal Title: Biol Reprod Volume: 81 Pages: 1002-1009
  • Peer Reviewed
• [Journal Article] Effects of diethylstilbestrol on ovarian follicle development in neonatal mice (2009)
  • Author(s): Kim, H., Hayashi, S., Chambon, P., Watanabe, H., Iguchi, T. & Sato, T.
  • Journal Title: Reproductive Toxicology Volume: 27 Pages: 55-62
  • Peer Reviewed
• [Journal Article] Effects of diethylstilbestrol on programmed oocyte death and induction of polyovular follicles in neonatal mouse ovaries. (2009)
  • Author(s): Kim H, Nakajima T, Hayashi S, Chambon P, Watanabe H, Iguchi T, Sato T.
  • Journal Title: Biol Reprod. 27 Pages: 1002-9
  • Peer Reviewed
• [Journal Article] Mono-(2-ethylhexyl) phthalate targets glycogen debranching enzyme and affects glycogen metabolism in rat testis (2009)
  • Author(s): C.Kuramori, Y.Hase, K.Hoshikawa, K.Watanabe, T.Nishi, T.Hishiki, T.Soga, A.Nashimoto, Y.Kabe, Y.Yamaguchi, H.Watanabe, K.Kataoka, M.Suematsu, H.Handa
  • Journal Title: Toxicol. Sci. 109 Pages: 143-51
  • Peer Reviewed
• [Journal Article] Pax6-5a promotes neuronal differentiation of murine embryonic stem cells (2009)
  • Author(s): N.Shimizu, H.Watanabe, J.Kubota, J.Wua, R.Saitoa, T.Yokoia, T.Erad, T.Iwatsubo, T.Watanabe, S.Nishina, N.Azumag, T.Katada, H.Nishina
  • Journal Title: Biol. Pharm. Bull. 32 Pages: 999-1003
  • NAID: 130000117177
  • Peer Reviewed
• [Presentation] オオミジンコにおけるRNA干渉法の開発 (2011)
  • Author(s): 加藤泰彦、渡邉肇
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 甲殻類における核内受容体の機能 (2011)
  • Author(s): 渡邉肇
  • Organizer: 環境ホルモン学会、第14回講演会シンポジウム
  • Place of Presentation: 東京大学山上会館
• [Presentation] Development of an RNA interference method in the cladoceran crustaceans, Daphnia magna (2011)
  • Author(s): Kato, Y., Shiga, Y., Tokishita, S., Kobayashi, K., Yamagata, H., Iguchi, T., & Watanabe, H.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] 環境によるミジンコの性決定機構 (2011)
  • Author(s): 加藤泰彦, 渡邉肇
  • Organizer: 第13回日本進化学会大会
  • Place of Presentation: 京都
• [Presentation] Development of an RNA interference method in the cladoceran crustacean Daphnia magna (2011)
  • Author(s): Kato, Y., Shiga, Y., Tokishita, S-I., & Watanabe, H
  • Organizer: 8th International Congress of Comparative Physiology and Biochemistry
  • Place of Presentation: Nagoya, Japan
• [Presentation] オオミジンコDaphnia magnaの性決定期における幼若ホルモン応答遺伝子の網羅的解析 (2011)
  • Author(s): 豊田賢治、宮川一志、小田重人、宮川信一、荻野由紀子、加藤泰彦、渡邉肇、鑪迫典久、井口泰泉
  • Organizer: 第82回日本動物学会大会
  • Place of Presentation: 旭川
• [Presentation] オミクス技術から環境を評価する (2010)
  • Author(s): Hajime Watanabe
  • Organizer: 自動車研究講演会
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2010-09-21
• [Presentation] 周生期エストロゲン曝露によるエピジェネティックな影響 (2010)
  • Author(s): 渡邉肇
  • Organizer: 日本内分泌学会
  • Place of Presentation: 前橋
  • Year and Date: 2010-04-25
• [Presentation] Epigenetic effect of endocrine disruptors (2010)
  • Author(s): Hajime Watanabe
  • Organizer: 14^<th> International Congress of Endocrinology
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2010-03-29
• [Presentation] Gefomic. apprcaches for evaluation of chemicals released into the environment. (2010)
  • Author(s): 渡邉肇
  • Organizer: フロンティア産業バイオシンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2010-02-09
• [Presentation] ゲノミクスデータのフェノタイプアンカリングの試み (2010)
  • Author(s): 渡邉肇
  • Organizer: Q-Bio(定量生物学)の会
  • Place of Presentation: 大阪
  • Year and Date: 2010-01-10
• [Presentation] Effects of Juvenile Hormone Agonists for Embryonic Development of Daphnia magna (2010)
  • Author(s): Toyota, K., Kato, Y., Miyagawa, S., Ogino, Y., Kobayashi, K., Oda, S., Watanabe, H. Tatarazako, S., & Iguchi, T.
  • Organizer: 環境ホルモン学会第13回研究発表会
  • Place of Presentation: 東京
• [Presentation] Sequence divergence and expression of a transformer gene in the branchiopod crustaceans, Daphnia magna (2010)
  • Author(s): Kato, Y., Kobayashi, K., Oda, S., Tatarazako, N., Watanabe, H. & Iguchi, T.
  • Organizer: Daphnia Genomics Consortium 2010
  • Place of Presentation: Leuven University,(Belgium)
• [Presentation] Molecular impact of juvenile hormone agonists on neonatal Daphnia magna (2010)
  • Author(s): Iguchi, T., Kato, Y., Miyagawa, S., Kobayashi, K., Oda, S., Tatarazako, N., & Watanabe, H.
  • Organizer: Daphnia Genomics Consortium 2010
  • Place of Presentation: Leuven University,(Belgium)
• [Presentation] 新生児期エストロゲン曝露によるエピジェネティックな影響 (2009)
  • Author(s): 渡邉肇
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] エレクトロポレーションを用いたオオミジンコにおけるGFP遺伝子の異所的発現 (2009)
  • Author(s): 加藤泰彦、小林かおる、小田重人、鑪迫典久、渡邉肇、井口泰泉
  • Organizer: 環境ホルモン学会第12回研究発表会
  • Place of Presentation: 東京
• [Presentation] Modulation of sex determination by juvenile hormones and their analogs in Daphnia magna (2009)
  • Author(s): Iguchi, T., Kato, Y., Watanabe, H., Oda, S., Tatarazako, N.
  • Organizer: International Conference on Comparative Endocrinology, Symposium Environmental Toxicology and Endocrine Disruption
  • Place of Presentation: Hong Kong, China
• [Presentation] エレクトロポレーションを用いたオオミジンコへの遺伝子導入 (2009)
  • Author(s): 加藤泰彦、小林かおる、渡邉肇、井口泰泉
  • Organizer: 第80回日本動物学会大会
  • Place of Presentation: 静岡
• [Book] In : Handbook of Systems Toxicology, Application of ecotoxicogenomics for understanding mode of action of chemicals and species extrapolation (2011)
  • Author(s): Watanabe, H., Kato, Y., and Iguchi, T.
  • Publisher: John Wiley & Sons, ltd.
• [Book] ミジンコのゲノム情報を利用した脱皮メカニズムの解析,脱皮と変態の生物学 (2011)
  • Author(s): 渡邉肇, 加藤泰彦
  • Publisher: 東海大学出版会
• [Book] 環境分析ガイドブック 2.7 化学物質の毒性試験 (2010)
  • Author(s): 渡邉肇(分担)
  • Publisher: 丸善(株)出版(印刷中)
• [Book] 環境分析ガイドブック 2.7 化学物質の毒性試験 (2010)
  • Author(s): 渡邉肇 (分担)
  • Publisher: 丸善(株)出版(印刷中)