Evaluation of the Degree of Maturation in the Regenerative Tissues by Analysis of Micro-Elastic Structure

• Project/Area Number: 21360123
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Intelligent mechanics/Mechanical systems
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: TAKAMIZAWA Keiichi 独立行政法人国立循環器病研究センター, 生体医工学部, 研究員 (10163312)
• Co-Investigator(Kenkyū-buntansha): KANDA Keiichi 京都府立医科大学, 心臓血管外科, 講師 (60295649)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2011: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2009: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
• Keywords: 再生医療 / 触覚顕微鏡 / 弾性率 / 生体組織 / 計測装置 / 生体顕微鏡 / バイオバルブ / バイオチューブ / タクタイルマッピング / 生体顕徴鏡

Research Abstract

We have been developed a scanning haptic microscope(SHM) for easy observation of the distribution of surface elasticity over a tissue slice sample. By improvement of SHM long time observation and wide area surface elasticity distribution imaging of the porcine arteries were enabled by soaking the sample in saline. Therefore, the differences in the elasticity under no load condition were revealed by visualization of local elastic structure at the extracellular matrix level in different segments of the canine aorta. SHM proved to be a powerful tool for precise evaluation of the degree of physical maturation after implantation of tissue-engineered tissues.

Research Products

• [Journal Article] Water-soluble argatroban for antithrombogenic surface coating of tissue-engineered cardiovascular tissues (2011)
  • Author(s): Nakayama Y, Yamaoka S, Yamanami M, Fujiwara M, Uechi M, Takamizawa K, Ishibashi-Ueda H, Nakamichi M, Uchida K, Watanabe T, Kanda K, Yaku H.
  • Journal Title: J Biomed Mater Res B Appl Biomater Volume: 99(2) Issue: 2 Pages: 420-30
  • DOI: 10.1002/jbm.b.31914
  • Peer Reviewed
• [Journal Article] Variations in local elastic modulus along the length of the aorta as observed by use of a scanning haptic microscope (SHM) (2011)
  • Author(s): 森脇健、村山嘉延, ら
  • Journal Title: Journal of Artificial Organs Volume: 14 Issue: 4 Pages: 276-283
  • DOI: 10.1007/s10047-011-0596-2
  • NAID: 10030339193
  • Peer Reviewed
• [Journal Article] A completely autologous valved conduit prepared in the open form of trileaflets(type VI biovalve): mold design and valve function in vitro (2011)
  • Author(s): Nakayama Y, Yahata Y, Yamanami M, Tajikawa T, Ohba K, Kanda K, Yaku H
  • Journal Title: J Biomed Mater Res B Appl Biomater Volume: 99(1) Issue: 1 Pages: 135-41
  • DOI: 10.1002/jbm.b.31880
  • Peer Reviewed
• [Journal Article] Long-term animal implantation study of biotube-autologous small-caliber vascular graft fabricated by in-body tissue architecture (2011)
  • Author(s): Watanabe T, Kanda K, Yamanami M, Ishibashi-Ueda H, Yaku H, Nakayama Y
  • Journal Title: J Biomed Mater Res B Appl Biomater Volume: 98(1) Issue: 1 Pages: 120-6
  • DOI: 10.1002/jbm.b.31841
  • Peer Reviewed
• [Journal Article] Preparation of well-defined poly(ether-ester) macromers : photogelation and biodegradability (2011)
  • Author(s): Yasuhide Nakayama
  • Journal Title: Acta Biomater Volume: 7 Issue: 4 Pages: 1496-1503
  • DOI: 10.1016/j.actbio.2010.11.023
  • Peer Reviewed
• [Journal Article] In-body optical stimulation formed connective tissue vascular grafts,"biotubes," with many capillaries and elastic fibers (2010)
  • Author(s): Oie T, Yamanami M, Ishibashi-Ueda H, Kanda K, Yaku H, Nakayama Y
  • Journal Title: J Artif Organs Volume: 13(4) Issue: 4 Pages: 235-40
  • DOI: 10.1007/s10047-010-0517-9
  • NAID: 10029565342
  • Peer Reviewed
• [Journal Article] Development of a completely autologous valved conduit with the sinus of Valsalva using in-body tissue architecture technology : a pilot study in pulmonary valve replacement in a beagle model (2010)
  • Author(s): Yamanami M, Yahata Y, Uechi M, Fujiwara M, Ishibashi-Ueda H, Kanda K, Watanabe T, Tajikawa T, Ohba K, Yaku H, Nakayama Y
  • Journal Title: Circulation Volume: 122(11) Issue: 11_suppl_1
  • DOI: 10.1161/circulationaha.109.922211
  • Peer Reviewed
• [Journal Article] Surface elasticity imaging of vascular tissues in a liquid environment by a scanning haptic microscope (2010)
  • Author(s): Oie T, Suzuki H, Murayama Y, Fukuda T, Omata S, Kanda K, Takamizawa K, Nakayama Y
  • Journal Title: J Artif Organs Volume: 13(2) Issue: 2 Pages: 121-5
  • DOI: 10.1007/s10047-010-0503-2
  • NAID: 10027706085
  • Peer Reviewed
• [Journal Article] Preparation of in-vivo tissue-engineered valved conduit with the sinus of Valsalva(type IV biovalve) (2010)
  • Author(s): Yamanami M, Yahata Y, Tajikawa T, Ohba K, Watanabe T, Kanda K, Yaku H, Nakayama Y
  • Journal Title: J Artif Organs Volume: 13(2) Issue: 2 Pages: 106-12
  • DOI: 10.1007/s10047-010-0491-2
  • NAID: 10027706030
  • Peer Reviewed
• [Journal Article] Autologous small-caliber "biotube" vascular grafts with argatroban loading : a histomorphological examination after implantation to rabbits (2010)
  • Author(s): Watanabe T, Kanda K, Ishibashi-Ueda H, Yaku H, Nakayama Y
  • Journal Title: J Biomed Mater Res B Appl Biomater Volume: 92(1) Issue: 1 Pages: 236-42
  • DOI: 10.1002/jbm.b.31510
  • Peer Reviewed
• [Journal Article] 3-Tesla magnetic resonance angiographic assessment of a tissue-engineered small-caliber vascular graft implanted in a rat (2010)
  • Author(s): Yamanami M, Yamamoto A, Iida H, Watanabe T, Kanda K, Yaku H, Nakayama Y
  • Journal Title: J Biomed Mater Res B Appl Biomater Volume: 92(1) Issue: 1 Pages: 156-60
  • DOI: 10.1002/jbm.b.31501
  • Peer Reviewed
• [Journal Article] Surface elasticity imaging of vascular tissues in a liquid environment by a scanning haptic microscope (2010)
  • Author(s): Tomonori Oie
  • Journal Title: J Artif Organs Volume: 13 Pages: 121-125
  • Peer Reviewed
• [Journal Article] Autologous small-caliber "biotube" vascular grafts with argatroban loading : a histomorphological examination after implantation to rabbits (2010)
  • Author(s): Taiji Watanabe
  • Journal Title: J Biomed Mater Res B Appl Biomater Volume: 92 Pages: 236-242
  • Peer Reviewed
• [Journal Article] Development of a completely autologous valved conduit with the sinus of Valsalva using in-body tissue architecture technology : a pilot study in pulmonary valve replacement in a beagle model (2010)
  • Author(s): Masashi Yamanami
  • Journal Title: Circulation Volume: 122
  • Peer Reviewed
• [Journal Article] Tactile mapping system : a novel imaging technology for surface topography and elasticity of tissues or organs (2009)
  • Author(s): Oie T, Suzuki H, Fukuda T, Murayama Y, Omata S, Kanda K, Nakayama Y
  • Journal Title: Innovations(Phila) Volume: 4(6) Issue: 6 Pages: 345-50
  • DOI: 10.1097/imi.0b013e3181c45bf9
  • Peer Reviewed
• [Journal Article] Preparation of a completely autologous trileaflet valve-shaped construct by in-body tissue architecture technology (2009)
  • Author(s): Nakayama Y, Yamanami M, Yahata Y, Tajikawa T, Ohba K, Watanabe T, Kanda K, Yaku H
  • Journal Title: J Biomed Mater Res B Appl Biomater Volume: 91(2) Issue: 2 Pages: 813-8
  • DOI: 10.1002/jbm.b.31460
  • Peer Reviewed
• [Journal Article] In vitro maturation of "biotube" vascular grafts induced by a 2-day pulsatile flow loading (2009)
  • Author(s): Huang H, Zhou YM, Ishibashi-Ueda H, Takamizawa K, Ando J, Kanda K, Yaku H, Nakayama Y
  • Journal Title: J Biomed Mater Res B Appl Biomater Volume: 91(1) Issue: 1 Pages: 320-8
  • DOI: 10.1002/jbm.b.31405
  • Peer Reviewed
• [Journal Article] Local elasticity imaging of vascular tissues using a tactile mapping system (2009)
  • Author(s): Oie T, Murayama Y, Fukuda T, Nagai C, Omata S, Kanda K, Yaku H, Nakayama Y
  • Journal Title: J Artif Organs Volume: 12(1) Issue: 1 Pages: 40-6
  • DOI: 10.1007/s10047-008-0440-5
  • NAID: 10025350678
  • Peer Reviewed
• [Journal Article] Faster and stronger vascular "Biotube" graft fabrication in vivo using a novel nicotine-containing mold (2009)
  • Author(s): Sakai O, Kanda K, Takamizawa K, Sato T, Yaku H, Nakayama Y
  • Journal Title: J Biomed Mater Res B Appl Biomater Volume: 90(1) Issue: 1 Pages: 412-20
  • DOI: 10.1002/jbm.b.31300
  • Peer Reviewed
• [Journal Article] Local Elasticity Imaging of Vascular Tissues Using a Tactile Mapping System (2009)
  • Author(s): Tomonori Oie
  • Journal Title: J Artif Organs 12 Pages: 40-46
  • Peer Reviewed
• [Journal Article] Faster and Stronger Vascular "Biotube" Graft Fabrication in Vivo Using a Novel Nicotine-contining Mold. (2009)
  • Author(s): Osamu Sakai
  • Journal Title: J Biomed Mater Res B Appl Biomater 90B Pages: 412-420
  • Peer Reviewed
• [Journal Article] Tactile Mapping System. A Novel Imaging Technology for Surface Topography and Elasticity of Tissues or Organs. (2009)
  • Author(s): Tomonori Oie
  • Journal Title: Innovations 4 Pages: 345-350
  • Peer Reviewed
• [Presentation] マイクロメカニクス的な再生度の評価手段としての走査型触覚顕微鏡の開発 (2011)
  • Author(s): 森脇健司、前川侑也、大家智憲、高見沢計一、福田達、村山嘉延、尾股定夫、上地正実、松井悠一、山南将志、神田圭一、中山泰秀
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京、京王プラザホテル
• [Presentation] 走査型触覚顕微鏡を用いた内圧負荷に伴う動脈壁内部の弾性率分布変化の観察 (2011)
  • Author(s): 森脇健司、前川侑也、大家智憲、高見沢計一、福田達、村山嘉延、尾股定夫、神田圭一、中山泰秀
  • Organizer: 第50回日本生体医工学会大会
  • Place of Presentation: 東京、東京電気大学
• [Presentation] Varietion of local elasticity alomg the length of the aorta as observed by a scanning haptic microscope(SHM) (2011)
  • Author(s): T. Moriwaki, T. Oie, K. Takamizawa, Y. Murayama, T. Fukuda, S. Omata, K. Kanda, Y. Nakayama
  • Organizer: 38th ESAO 2011(ヨーロッパ人工臓器学会)
  • Place of Presentation: ポルトガル、ポルト
• [Presentation] 生体作動類似環境下における動脈壁内弾性率の走査型触覚顕微鏡観察 (2011)
  • Author(s): 高見沢計一、森脇健司、大家智憲、福田達、村山嘉延、尾股定夫、中山泰秀
  • Organizer: 第49回日本人工臓器学会大会
  • Place of Presentation: 東京、都市センターホテル
• [Presentation] 生体作動類似環境下における動脈壁内弾性率の走査型触覚顕微鏡観察 (2011)
  • Author(s): 高見沢計一
  • Organizer: 第49回日本人工臓器学会
  • Place of Presentation: 東京都都市センターホテル
• [Presentation] Varietion of local elasticity alomg the length of the aorta as observed by a scanning haptic microscope (SHM) (2011)
  • Author(s): T.Moriwaki
  • Organizer: 第38回ヨーロッパ人工臓器学会
  • Place of Presentation: ポルト(ポルトガル)
• [Presentation] 走査型触覚顕微鏡(SHM)を用いた細胞外マトリックスレベルでの弾性率分布の比較 (2010)
  • Author(s): 森脇健司、大家智憲、高見沢計一、福田達、村山嘉延、尾股定夫、神田圭一、中山泰秀
  • Organizer: 第48回日本人工臓器学会
  • Place of Presentation: 仙台国際センター
• [Presentation] Evaluation of mechanical properties of in vivo tissue-engineered prosthetic heart valves (2010)
  • Author(s): Tomonori Oie
  • Organizer: European Society for Artificial Organs
  • Place of Presentation: Macedonia, Skopje
• [Presentation] EVALUATION OF MECHANICAL PROPERTIES OF IN VIVO TISSUE-ENGINEERED PROSTHETIC HEART VALVES,"BIOVALVES" (2009)
  • Author(s): Oie T, Moriwaki T, Murayama Y, Omata S, Uechi M, Yamanami M, Kanda K, Nakayama Y
  • Organizer: 37th Congress European Society for Artificial Organs
  • Place of Presentation: Macedonia, Skopje
• [Presentation] Development of tactile mapping system(TMS): Surface elasticity mapping of arteries in water (2009)
  • Author(s): H. Suzuki, T. Oie, Y. Murayama, S. Omata, K. Kanda, Y. Nakayama
  • Organizer: 36th Congress European Society for Artificial Organs
  • Place of Presentation: France, Complegne
• [Presentation] 水中での弾性率マッピングによる血管組織内のマトリックスの分布観察 (2009)
  • Author(s): 鈴木寿人、大家智憲、福田達、村山嘉延、尾股定夫、神田圭一、中山泰秀
  • Organizer: 第47回日本人工臓器学会大会
  • Place of Presentation: 新潟コンベンションセンター
• [Presentation] Development of Tactile Mapping System(TMS) : Surface Elasticity Mapping of Arteries in Water. (2009)
  • Author(s): Hisato Suzuki
  • Organizer: European Society for Artificial Organs
  • Place of Presentation: France, Complegne
• [Presentation] 循環器系再生医療におけるTMSの応用と可能性
  • Author(s): 大家智憲、鈴木寿人、矢羽田侑希、山南将志、高見沢計一、渡辺太治、神田圭一、福田達、村山嘉延、尾股定夫、中山泰秀
  • Organizer: 第24回生体・生理工学シンポジウム
  • Place of Presentation: 東北大学