Plant membrane traffic-generality and uniqueness of functions and molecular mechanisms-

• Project/Area Number: 21370016
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The University of Tokyo
• Principal Investigator: UEDA Takashi 東京大学, 大学院・理学系研究科, 准教授 (10311333)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
• Keywords: オルガネラ / 細胞壁 / Rab GTPase / ARA6 / ARA7 / endosome / 膜交通 / 多様化 / 進化 / RAB GTPase

Research Abstract

We carried out studies on molecular mechanisms and physiological functions of plant membrane trafficking. Supported by this research grant, we succeeded in identification of a plant-unique endosomal trafficking pathway, which involves two plant-unique molecules, ARA6 and VAMP727. We further performed the research using the liverwort, Marchantia polymorpha, for information on a diversity and evolution of the plant membrane trafficking system.

Research Products

• [Journal Article] Statistical organelle dissection of Arabidopsis guard cells using image database LIPS. (2012)
  • Author(s): Higaki, et al.
  • Journal Title: Sci Rep. Volume: 2 Issue: 1 Pages: 405-405
  • DOI: 10.1038/srep00405
  • Peer Reviewed
• [Journal Article] Microtubule stability affects the unique motility of F-actin in Marchantia polymorpha (2012)
  • Author(s): Era, A., Kutsuna, N., Higaki, T., Hasezawa S., Nakano, A., and Ueda, T.
  • Journal Title: J. Plant Res. Volume: (in press)
  • NAID: 10031145620
  • Peer Reviewed
• [Journal Article] Qa-SNAREs localized to the trans-Golgi network regulate multiple transport pathways and extracellular disease resistance in plants (2012)
  • Author(s): Uemura, et al.
  • Journal Title: PNAS Volume: 109(5) Issue: 5 Pages: 1784-9
  • DOI: 10.1073/pnas.1115146109
  • Peer Reviewed
• [Journal Article] An endosomal trafficking pathwayregulated by the plant-unique RAB5, ARA6 (2012)
  • Author(s): Ebine, K., Miyakawa, N., Fujimoto, M., Uemura, T., Nakano, A., and Ueda, T.
  • Journal Title: Small GTPases Volume: 3 Pages: 1-5
  • URL: http://www.landesbioscience.com/journals/smallgtpases/article/18299/
  • Peer Reviewed
• [Journal Article] Dynamic behavior of clathrin in Arabidopsis thaliana unveiled by live imaging (2012)
  • Author(s): Ito E, Fujimoto M, Ebine K, Uemura T, Ueda T, Nakano A
  • Journal Title: Plant Journal Volume: 69 Issue: 2 Pages: 204-216
  • DOI: 10.1111/j.1365-313x.2011.04782.x
  • Peer Reviewed
• [Journal Article] 細胞壁資材の細胞内輸送-植物の膜交通と細胞壁-. (2012)
  • Author(s): 藤本優, 上田貴志
  • Journal Title: 遺伝 Volume: Vol.66 Pages: 47-52
• [Journal Article] 細胞壁資材の細胞内輸送-植物の膜交通と細胞壁- (2012)
  • Author(s): 藤本優, 上田貴志
  • Journal Title: 遺伝 Volume: 66 Pages: 47-52
• [Journal Article] AUNGUSTIFOLIA, a plant homolog of CtBP/BARS, functions outside the nucleus (2011)
  • Author(s): Minamisawa N, Sato M, Cho K-H, Ueno H, Takeuchi K, Kajiwara M, Yamato KT, Ohyama K, Toyooka K, Kim G-T, Horiguchi G, Takano H, Ueda T, and Tsukaya H
  • Journal Title: Plant J Volume: 68 Issue: 5 Pages: 788-799
  • DOI: 10.1111/j.1365-313x.2011.04731.x
  • Peer Reviewed
• [Journal Article] Qualitative difference between "bulb" membranes and other vacuolar membranes (2011)
  • Author(s): Saito, C.
  • Journal Title: Plant Signal.Behav. Volume: 6 Issue: 12 Pages: 1914-1917
  • DOI: 10.4161/psb.6.12.18061
  • Peer Reviewed
• [Journal Article] The occurrence of 'bulbs', a complex configuration of the vacuolar membrane, is affected by mutations of vacuolar SNARE and phospholipase in Arabidopsis (2011)
  • Author(s): Saito, et al.
  • Journal Title: Plant Journal Volume: 68(1) Issue: 1 Pages: 64-73
  • DOI: 10.1111/j.1365-313x.2011.04665.x
  • Peer Reviewed
• [Journal Article] A membrane trafficking pathway regulated by the plant-specific RAB GTPase ARA6 (2011)
  • Author(s): Ebine K, Fujimoto M, Okatani Y, Nishiyama T, Gob T, Ito E, Dainobu T, Nishitani A, Uemura T, Sato MH, Thordal-Christensen H, Tsutsumi N, Nakano A, Ueda T
  • Journal Title: Nature Gell Biology Volume: 13 Issue: 7 Pages: 853-859
  • DOI: 10.1038/ncb2270
  • Peer Reviewed
• [Journal Article] 陸上植物固有の膜交通制御因子が植物の新たな細胞内輸送経路を開拓した (2011)
  • Author(s): 海老根一生, 上田貴志
  • Journal Title: ライフサイエンス新着論文レビュー
  • URL: http://first.lifesciencedb.jp/archives/3119
• [Journal Article] ADP-ribosylation factor (ARF), guanine nucleotide exchange factor (GEF), and GAPase-acticating protein (GAP) function in endocytosis of plant cells (2010)
  • Author(s): Naramoto, S., Kleine-Vehn, J., Robert, S., Fujimoto, M., Dainobu, T., Paciorek, T., Ueda, T., Nakano, A., Van Montagu, M. C. E., Fukuda, H., Friml, J
  • Journal Title: Proc. Natl. Acad. Sci., USA Volume: 107 Issue: 50 Pages: 21890-21895
  • DOI: 10.1073/pnas.1016260107
  • Peer Reviewed
• [Journal Article] Vacuolar/prevacuolar compartment Qa-SNAREs, VAM3/SYP22 and PEP12/SYP21 have interchangeable functions in Arabidopsis (2010)
  • Author(s): Uemura, T., Morita, TM., Ebine, K., Okatani, Y., Yano, D., Saito, C., Ueda, T., and Nakano, A.
  • Journal Title: Plant J. Volume: 64 Issue: 5 Pages: 864-873
  • DOI: 10.1111/j.1365-313x.2010.04372.x
  • Peer Reviewed
• [Journal Article] GDP-Bound and Nucleotide-Free Intermediates of the Guanine Nucleotide Exchange in the Rab5/Vps9 system (2010)
  • Author(s): Uejima, T., Ihara, K., Goh, T., Ito, E., Sunada, M., Ueda, T., Nakano, A., and Wakatsuki, S.
  • Journal Title: J. Biol. Chem. Volume: 285 Issue: 47 Pages: 36689-36697
  • DOI: 10.1074/jbc.m110.152132
  • Peer Reviewed
• [Journal Article] Arabidopsis dynamin-related proteins DRP2B and DRP1A participate together in clathrin-coated vesicle formation during endocytosis (2010)
  • Author(s): M. Fujimoto, S. Arimura, T. Ueda, H. Takanashi, Y. Hayashi, A. Nakano, and N. Tsutsumi
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 107 Issue: 13 Pages: 6094-6099
  • DOI: 10.1073/pnas.0913562107
  • Peer Reviewed
• [Journal Article] The Hop/Stil-Hsp90 Chaperone Complex Facilitates the Maturation and Transport of a PAMP Receptor in Rice Innate Immunity (2010)
  • Author(s): Chen, L., Hamada, S., Fujiwara, M., Zhu, T., Thao, NP., Wong, HL., Krishna, P., Ueda, T., Kaku, H., Shibuya, N., Kawasaki, T., and Shimamoto, K.
  • Journal Title: Cell Host Microbe Volume: 7 Issue: 3 Pages: 185-196
  • DOI: 10.1016/j.chom.2010.02.008
  • Peer Reviewed
• [Journal Article] Endocytosis Restricts Arabidopsis KNOLLE Syntaxin To The Cell Division Plane During Late Cytokinesis (2010)
  • Author(s): Boutte, Y., Rosa, MSF., Men, S., Chow, C., Ebine, K., Gustavsson, A., Johansson, L., Ueda, T., Moore, I., Jurgens, G., Grebe, M.
  • Journal Title: EMBO J. Volume: 29 Issue: 3 Pages: 546-558
  • DOI: 10.1038/emboj.2009.363
  • Peer Reviewed
• [Journal Article] 植物のSNARE複合体と膜融合 (2010)
  • Author(s): 上田貴志
  • Journal Title: 生体の科学 Volume: Vol.61 Pages: 269-275
• [Journal Article] Vacuolar/prevacuolar compartment Qa-SNAREs, VAM3/SYP22 and PEP1 2/SYP21 have interchangeable functions in Arabidopsis. (2010)
  • Author(s): Uemura T, et al.
  • Journal Title: Plant J. Volume: 64 Pages: 864-873
  • Peer Reviewed
• [Journal Article] GDP-Bound and Nucleotide-Free Intermediates of the Guanine Nucleotide Exchange in the Rab5/Vns9 system. (2010)
  • Author(s): Uejima, et al.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 36689-36697
  • Peer Reviewed
• [Journal Article] Arabidopsis dynamin-related proteins DRP2B and DRP1A participate together in clathrin-coated vesicle formation during endocytosis. (2010)
  • Author(s): Fujimoto, M., et al.
  • Journal Title: Proc Natl Acad Sci USA. (印刷中)
  • Peer Reviewed
• [Journal Article] Visualization of mitochondrial and apicoplast nucleoids in the human malaria parasite Plasmodium falciparum by SYBR Green I and PicoGreen staining. (2010)
  • Author(s): Sano-Maeda, K., et al.
  • Journal Title: Cytologia (印刷中)
  • Peer Reviewed
• [Journal Article] The Hop/Sti1-Hsp90 Chaperone Complex Facilitates the Maturation and Transport of a PAMP Receptor in Rice Innate Immunity. (2010)
  • Author(s): Chen, L., et al.
  • Journal Title: Cell Host Microbe 7 Pages: 185-196
  • Peer Reviewed
• [Journal Article] Endocytosis Restricts Arabidopsis KNOLLE Syntaxin To The Cell Division Plane During Late Cytokinesis. (2010)
  • Author(s): Boutte, Y., et al.
  • Journal Title: EMBO J. 29 Pages: 546-558
  • Peer Reviewed
• [Journal Article] Application of Lifeact Reveals F-Actin Dynamics in Arabidopsis thaliana and the Liverwort, Marchantia polymorpha (2009)
  • Author(s): Era, A., Tominaga, M., Ebine, K., Awai, C., Saito, C., Ishizaki, K., Yamato, TK., Kohchi, T., Nakano, A., and Ueda, T.
  • Journal Title: Plant Cell Physiol. Volume: 50 Issue: 6 Pages: 1041-1048
  • DOI: 10.1093/pcp/pcp055
  • NAID: 10027340574
  • Peer Reviewed
• [Journal Article] Visualization of Mitochondrial and Apicoplast Nucleoids in the Human Malaria Parasite Plasmodium falciparum by SYBR Green I and PicoGreen Staining (2009)
  • Author(s): Sano-Maeda, K., Sato, S., Ueda, T., Yui, R., Itho, K., Hata, M., Nakano, A., Kita, K., Murakami-Murofushi, K. and Sasaki, N.
  • Journal Title: CYTOLOGIA Volume: 74 Issue: 4 Pages: 449-455
  • DOI: 10.1508/cytologia.74.449
  • NAID: 10026186344
  • ISSN: 0011-4545, 1348-7019
  • Peer Reviewed
• [Journal Article] Phosphoinositide-dependent regulation of VAN3 ARF-GAP localization and activity essential for vascular tissue continuity in plants (2009)
  • Author(s): Naramoto, S., Sawa, S., Koizumi, K., Uemura, T., Ueda, T., Friml, J., Nakano, A., Fukuda, H
  • Journal Title: Development Volume: 136 Issue: 9 Pages: 1529-1538
  • DOI: 10.1242/dev.030098
  • Peer Reviewed
• [Journal Article] Arabidopsis dynamin-related proteins DRP3A and DRP3B are functionally redundant in mitochondrial fission but have distinct roles in peroxisomal fission (2009)
  • Author(s): Fujimoto, M., Arimura, S., Mano, S., Kondo, M., Saito, C., Ueda, T., Nakazono, M., Nakano, A., Nishimura, M. and Tsutsumi, N.
  • Journal Title: Plant J. Volume: 58 Issue: 3 Pages: 388-400
  • DOI: 10.1111/j.1365-313x.2009.03786.x
  • Peer Reviewed
• [Journal Article] Function of RAB and SNARE members in plant life (2009)
  • Author(s): Saito, C. and Ueda, T.
  • Journal Title: Int. Rev. Cell Mol. Biol. Volume: 274 Pages: 183-233
  • DOI: 10.1016/s1937-6448(08)02004-2
  • ISBN: 9780123748058
  • Peer Reviewed
• [Journal Article] Unique mechanism of plant endocytic/vacuolar transport pathways (2009)
  • Author(s): Ebine, K. and Ueda T.
  • Journal Title: J. Plant Res. Volume: 122 Issue: 1 Pages: 21-30
  • DOI: 10.1007/s10265-008-0200-x
  • NAID: 10025178851
  • Peer Reviewed
• [Journal Article] Application of Lifeact Reveals F-Actin Dynamics in Arabidopsis thaliana and the Liverwort, Marchantia polymorpha. (2009)
  • Author(s): Era, A., et al.
  • Journal Title: Plant Cell Physiol. 50 Pages: 1041-1048
  • Peer Reviewed
• [Journal Article] Phosphoinositide-dependent regulation of VAN3 ARF-GAP localization and activity essential for vascular tissue continuity in plants. (2009)
  • Author(s): Naramoto, S., et al.
  • Journal Title: Development 136 Pages: 1529-1538
  • Peer Reviewed
• [Presentation] How plants acquired novel membrane trafficking pathways (2011)
  • Author(s): 上田貴志
  • Organizer: 第84回生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-24
• [Presentation] How plants acquired novel membrane trafficking pathways (2011)
  • Author(s): 上田貴志
  • Organizer: 第84回生化学会大会
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2011-09-24
• [Presentation] 植物はいかに独自の膜交通システムを構築したか (2011)
  • Author(s): 上田貴志
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-18
• [Presentation] 植物はいかに独自の膜交通システムを構築したか (2011)
  • Author(s): 上田貴志
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2011-09-18
• [Presentation] Diversification of post-Golgi trafficking pathways among land plants. (2011)
  • Author(s): Takashi Ueda
  • Organizer: XVIII International Botanical Congress
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2011-07-25
• [Presentation] Diversification of post-Golgi trafficking pathways among land plants (2011)
  • Author(s): Takashi Ueda
  • Organizer: XVIII International Botanical Congress
  • Place of Presentation: Melbourne, Australia(招待講演)
  • Year and Date: 2011-07-25
• [Presentation] How plants have acquired unique endosomal trafficking pathways (2010)
  • Author(s): Takashi Ueda
  • Organizer: Biochemistry and Molecular Biology 2010
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2010-12-09
• [Presentation] How plants have acquired unique endosomal trafficking pathways. (2010)
  • Author(s): Takashi Ueda
  • Organizer: Biochemistry and Molecular Biology 2010
  • Place of Presentation: Kobe, Japan 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] 可視化で探る植物膜交通経路の多様性 (2010)
  • Author(s): 上田貴志
  • Place of Presentation: 奈良
  • Year and Date: 2010-11-25
• [Presentation] Plant-unique endosomal trafficking pathways regulated by ARA6. (2010)
  • Author(s): Takashi Ueda
  • Organizer: Plant Science Communications 2010, The 26^<nd> NIBB-MPI joint symposium
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2010-11-16
• [Presentation] Plant-unique endosomal trafficking pathways regulated by ARA6. (2010)
  • Author(s): Takashi Ueda
  • Organizer: The 2^<nd> NIBB-MPI joint symposium
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2010-11-16
• [Presentation] ゼニゴケで植物膜交通研究の新地平を拓けるか (2010)
  • Author(s): 上田貴志
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 愛知
  • Year and Date: 2010-09-10
• [Presentation] 植物に学ぶ細胞内膜交通網の多様性獲得機構 (2010)
  • Author(s): 上田貴志
  • Organizer: 第62会日本細胞生物学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-20
• [Presentation] 植物の膜交通-分子機構と高次機能発現における役割の研究- (2010)
  • Author(s): 上田貴志
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-19
• [Presentation] 植物の膜交通 ～分子機構と高次機能発現における役割の研究～ (2010)
  • Author(s): 上田貴志
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-19
• [Presentation] 植物に学ぶ膜交通多様化機構-その普遍性と独自性に迫る- (2009)
  • Author(s): 上田貴志
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/hasseipl/HP/index.html
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/hasseipl/HP/index.html
• [Patent(Industrial Property Rights)] 植物特異的膜交通経路の活性化による環境ストレス耐性植物の開発 (2011)
  • Inventor(s): 上田貴志,稲田のりこ
  • Industrial Property Rights Holder: 東京大学,奈良先端科学技術大学院大学
  • Filing Date: 2011-11-11
  • Overseas
• [Patent(Industrial Property Rights)] 植物特異的膜交通経路の活性化による環境ストレス耐性植物の開発 (2011)
  • Inventor(s): 上田貴志, 稲田のりこ
  • Industrial Property Rights Holder: 東京大学,奈良先端科学技術大学院大学
  • Filing Date: 2011-11-11
  • Overseas