Single molecule FRET study for the walking mechanism of molecular motor kinesin

• Project/Area Number: 21370066
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: TOMISHIGE Michio 東京大学, 大学院・工学系研究科, 准教授 (50361530)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2010: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2009: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
• Keywords: 一分子計測 / 蛍光共鳴エネルギー移動 / 分子機械 / 分子モーター / 構造変化 / 細胞内輸送 / 生物物理 / 一分子計測(SMD) / ナノマシン

Research Abstract

Molecular motor kinesin walks along microtubules by alternately moving two motor heads. We hypothesized based on atomic structural models that the neck linkers connecting two heads are essential for the coordination between heads. We tested this hypothesis by observing at the single molecule level the movements and structural changes of the mutant kinesins whose tension posed to the neck linker were reduced or abolished and provided the structural basis to explain the walking mechanism of kinesin.

Research Products

• [Presentation] Structural basis for the coordinated processive movement of kinesin motor protein, The17th International Biophysics Congress, Symposium (2011)
  • Author(s): Michio Tomishige
  • Organizer: Cytoskeleton and motor dynamics
  • Place of Presentation: Beijing, China
  • Year and Date: 2011-11-01
• [Presentation] Structural basisfor the coordinated walking mechanism of kinesin motor protein, IMS Symposium on"Recent Development of Spectroscopy and Spatial and Temporal Hierarchical Structures in Molecular Science", (2011)
  • Author(s): Michio Tomishige
  • Organizer: Institute for Molecular Science
  • Place of Presentation: Institute for Molecular Science, Okazaki
  • Year and Date: 2011-10-18
• [Presentation] キネシンの協調的な二足歩行運動の仕組みとその制御 (2011)
  • Author(s): 富重道雄
  • Organizer: 第63回日本細胞生物学会シンポジウム「細胞内で協調して活躍するモーター分子」
  • Place of Presentation: 北海道大学
  • Year and Date: 2011-06-27
• [Presentation] Structural basis for the coordinated processive movement of kinesin-1 studied by structural and single molecule analyses (2011)
  • Author(s): Tsukasa Makino
  • Organizer: 55th Annual Meeting of Biophysical Society
  • Place of Presentation: Baltimore, MD, USA
  • Year and Date: 2011-03-06
• [Presentation] Forward stepping mechanism of kinesin-1 studied using asymmetrically-joined two-headed monome (2011)
  • Author(s): Hiroshi Isojima
  • Organizer: 55th Annual Meeting of Biophysical Society
  • Place of Presentation: Baltimore, MD, USA
  • Year and Date: 2011-03-06
• [Presentation] Structural basis for the coordinated processive movement of kinesin-1 studied by structural and single molecule analyses (2011)
  • Author(s): Tsukasa Makino
  • Organizer: 1st Korea University-The University of Tokyo Joint Workshop on Bio-Soft Matter
  • Place of Presentation: Koshiba Hall, Univ.of Tokyo
  • Year and Date: 2011-02-22
• [Presentation] Forward stepping mechanism of kinesin-1 studied using asymmetrically-joined two-headed monomer (2011)
  • Author(s): Hiroshi Isojima
  • Organizer: 1st Korea University-The University of Tokyo Joint Workshop on Bio-Soft Matter
  • Place of Presentation: Koshiba Hall, Univ.of Tokyo
  • Year and Date: 2011-02-22
• [Presentation] Strain-sensing mechanism of kinesin-1 as studied by disulfide-crosslinking of the neck linker (2011)
  • Author(s): Yamato Niitani
  • Organizer: 1st Korea University-The University of Tokyo Joint Workshop on Bio-Soft Matter
  • Place of Presentation: Koshiba Hall, Univ.of Tokyo
  • Year and Date: 2011-02-22
• [Presentation] キネシン頭部の選択的な前方着地におけるネックリンカーの役割 (2010)
  • Author(s): 磯島広
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: アスティとくしま
  • Year and Date: 2010-11-01
• [Presentation] キネシン1モータードメインのヌクレオチドフリー状態での結晶構造解析 (2010)
  • Author(s): 牧野司
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: アスティとくしま
  • Year and Date: 2010-10-30
• [Presentation] Structural basis for the coordinated movement of kinesin motor protein (2010)
  • Author(s): Michio Tomishige
  • Organizer: 1st HSFP AlumniMeeting - Japan
  • Place of Presentation: Yayoi Auditorium-Annex, Univ. of Tokyo
  • Year and Date: 2010-10-09
• [Presentation] Structural basis for the coordinated movement of kinesin motor protein (2010)
  • Author(s): Michio Tomishige
  • Organizer: 1st HSFP Alumni Meeting-Japan
  • Place of Presentation: Yayoi Auditorium-Anne, Univ.of Tokyo
  • Year and Date: 2010-10-09
• [Presentation] ジスルフィド架橋法を用いたキネシン1のネックリンカーのストレインセンサーとしての役割の研究 (2010)
  • Author(s): 新谷大和
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学川内キャンパス
  • Year and Date: 2010-09-21
• [Presentation] キネシン1の協調的な二足歩行運動を可能にする構造基盤の解析 (2010)
  • Author(s): 牧野司
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学川内キャンパス
  • Year and Date: 2010-09-20
• [Presentation] キネシン1が選択的に前方にステップする仕組み (2010)
  • Author(s): 磯島広
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学川内キャンパス
  • Year and Date: 2010-09-20
• [Presentation] 結晶構造解析と一分子計測法の融合により明らかになったモータータンパク質キネシンの二足歩行メカニズム (2010)
  • Author(s): 牧野司
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2010-06-16
• [Presentation] キネシン頭部の選択的な前方着地におけるネックリンカーの役割 (2010)
  • Author(s): 磯島広
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2010-06-16
• [Presentation] Structural basis for the coordinated processive movement of kinesin, EMBO Conference Series (2010)
  • Author(s): Michio Tomishige
  • Organizer: Microtubules: Structure, Regulation and Functions
  • Place of Presentation: EMBL Advanced Training Centre, Heidelberg, Germany
  • Year and Date: 2010-06-05
• [Presentation] Microtubules : Structure, Regulation and Functions (2010)
  • Author(s): Michio Tomishige
  • Organizer: Structural basis for the coordinated processive movement of kinesin EMBO Conference Series "Microtubules : Structure, Regulation and Functions
  • Place of Presentation: EMBL Advanced Training Centre, Heidelberg, Germany
  • Year and Date: 2010-06-05
• [Presentation] キネシン頭部の選択的な前方着地におけるネックリンカーの役割 (2010)
  • Author(s): 磯島広
  • Organizer: 第10回東京大学生命科学シンポジウム
  • Place of Presentation: 東京大学本郷キャンパス
  • Year and Date: 2010-05-01
• [Presentation] Structural basis for the mechanochemical coupling of kinesin-1 revealed by crystal structural and biochemical analyses (2010)
  • Author(s): Tsukasa Makino
  • Organizer: 54th Annual Meeting of Biophysical Society
  • Place of Presentation: Moscone Center米国サンフランシスコ
  • Year and Date: 2010-02-22
• [Presentation] Optimal size of the neck linker is important for the coordinated nrocessive movement of kinesin-1 (2010)
  • Author(s): Hiroshi Isojima
  • Organizer: 54th Annual Meeting of Biophysical Society
  • Place of Presentation: Moscone Center米国サンフランシスコ
  • Year and Date: 2010-02-22
• [Presentation] Single molecule observation of structural changes of kinesin motor protein (2009)
  • Author(s): Michio Tomishige
  • Organizer: International Symposium onWatching Biomolecules in Action
  • Place of Presentation: ライフサイエンスセンタ ー、大阪
  • Year and Date: 2009-12-15
• [Presentation] Single molecule observation of structural changes of kinesin motor protein (2009)
  • Author(s): Michio Tomishige
  • Organizer: International Symposium on Watching Biomolecules in Action
  • Place of Presentation: ライフサイエンスセンター、大阪
  • Year and Date: 2009-12-15
• [Presentation] Role of the neck linker on the preferential forward stepping of kinesin (2009)
  • Author(s): Hiroshi Isojima
  • Organizer: International Symposium on Watching Biomolecules in Action
  • Place of Presentation: ライフサイエンスセンター、大阪
  • Year and Date: 2009-12-15
• [Presentation] Structural basis for the coordinated processive movement of microtubule-based motor kinesin, International Symposium (2009)
  • Author(s): Michio Tomishige
  • Organizer: Innovative Nanoscience ofSupermolecular Motor Proteins Working in Biomembranes
  • Place of Presentation: 京都大学芝蘭会館
  • Year and Date: 2009-09-09
• [Presentation] Structural basis for the coordinated processive movement of microtubule-based motor kinesin (2009)
  • Author(s): Michio Tomishige
  • Organizer: International Symposium "Innovative Nanoscience of Supermolecular Motor Proteins Working in Biomembranes"
  • Place of Presentation: 京都大学 芝蘭会館
  • Year and Date: 2009-09-09
• [Presentation] キネシンの協調的二足歩行メカニズム
  • Author(s): 富重道雄
  • Organizer: 第１回分子モーター討論会
  • Place of Presentation: 東京大学本郷キャンパス
  • Invited
• [Presentation] Structural basis for the coordinated walking mechanism of kinesin motor protein
  • Author(s): Michio Tomishige
  • Organizer: IMS Symposium on "Recent Development of Spectroscopy and Spatial and Temporal Hierarchical Structures in Molecular Science", Institute for Molecular Science
  • Place of Presentation: Institute for Molecular Science, Okazaki
  • Invited
• [Presentation] Structural basis for the coordinated processive movement of kinesin motor protein
  • Author(s): Michio Tomishige
  • Organizer: The 17th International Biophysics Congress, Symposium "Cytoskeleton and motor dynamics"
  • Place of Presentation: Beijing, China
  • Invited
• [Presentation] Strain-dependent regulation of kinesin-1 as studied by disulfide-crosslinking of the neck linker
  • Author(s): 新谷大和
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫県立大学姫路書写キャンパス
• [Presentation] Role of the neck linker on the front head gating mechanism of kinesin-1
  • Author(s): Xiao Ling
  • Organizer: The 17th International Biophysics Congress
  • Place of Presentation: Beijing, China
• [Presentation] The neck linker of kinesin-1 functions as a regulator of ATP hydrolysis reaction
  • Author(s): Xiao Ling
  • Organizer: 56th Annual Meeting of Biophysical Society
  • Place of Presentation: San Diego, CA, USA