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Single molecule FRET study for the walking mechanism of molecular motor kinesin

Research Project

Project/Area Number 21370066
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionThe University of Tokyo

Principal Investigator

TOMISHIGE Michio  東京大学, 大学院・工学系研究科, 准教授 (50361530)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2010: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2009: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Keywords一分子計測 / 蛍光共鳴エネルギー移動 / 分子機械 / 分子モーター / 構造変化 / 細胞内輸送 / 生物物理 / 一分子計測(SMD) / ナノマシン
Research Abstract

Molecular motor kinesin walks along microtubules by alternately moving two motor heads. We hypothesized based on atomic structural models that the neck linkers connecting two heads are essential for the coordination between heads. We tested this hypothesis by observing at the single molecule level the movements and structural changes of the mutant kinesins whose tension posed to the neck linker were reduced or abolished and provided the structural basis to explain the walking mechanism of kinesin.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (33 results)

All 2011 2010 2009 Other

All Presentation (33 results) (of which Invited: 3 results)

  • [Presentation] Structural basis for the coordinated processive movement of kinesin motor protein, The17th International Biophysics Congress, Symposium2011

    • Author(s)
      Michio Tomishige
    • Organizer
      Cytoskeleton and motor dynamics
    • Place of Presentation
      Beijing, China
    • Year and Date
      2011-11-01
    • Related Report
      2011 Final Research Report
  • [Presentation] Structural basisfor the coordinated walking mechanism of kinesin motor protein, IMS Symposium on"Recent Development of Spectroscopy and Spatial and Temporal Hierarchical Structures in Molecular Science",2011

    • Author(s)
      Michio Tomishige
    • Organizer
      Institute for Molecular Science
    • Place of Presentation
      Institute for Molecular Science, Okazaki
    • Year and Date
      2011-10-18
    • Related Report
      2011 Final Research Report
  • [Presentation] キネシンの協調的な二足歩行運動の仕組みとその制御2011

    • Author(s)
      富重道雄
    • Organizer
      第63回日本細胞生物学会シンポジウム「細胞内で協調して活躍するモーター分子」
    • Place of Presentation
      北海道大学
    • Year and Date
      2011-06-27
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Structural basis for the coordinated processive movement of kinesin-1 studied by structural and single molecule analyses2011

    • Author(s)
      Tsukasa Makino
    • Organizer
      55th Annual Meeting of Biophysical Society
    • Place of Presentation
      Baltimore, MD, USA
    • Year and Date
      2011-03-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] Forward stepping mechanism of kinesin-1 studied using asymmetrically-joined two-headed monome2011

    • Author(s)
      Hiroshi Isojima
    • Organizer
      55th Annual Meeting of Biophysical Society
    • Place of Presentation
      Baltimore, MD, USA
    • Year and Date
      2011-03-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] Structural basis for the coordinated processive movement of kinesin-1 studied by structural and single molecule analyses2011

    • Author(s)
      Tsukasa Makino
    • Organizer
      1st Korea University-The University of Tokyo Joint Workshop on Bio-Soft Matter
    • Place of Presentation
      Koshiba Hall, Univ.of Tokyo
    • Year and Date
      2011-02-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Forward stepping mechanism of kinesin-1 studied using asymmetrically-joined two-headed monomer2011

    • Author(s)
      Hiroshi Isojima
    • Organizer
      1st Korea University-The University of Tokyo Joint Workshop on Bio-Soft Matter
    • Place of Presentation
      Koshiba Hall, Univ.of Tokyo
    • Year and Date
      2011-02-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Strain-sensing mechanism of kinesin-1 as studied by disulfide-crosslinking of the neck linker2011

    • Author(s)
      Yamato Niitani
    • Organizer
      1st Korea University-The University of Tokyo Joint Workshop on Bio-Soft Matter
    • Place of Presentation
      Koshiba Hall, Univ.of Tokyo
    • Year and Date
      2011-02-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] キネシン頭部の選択的な前方着地におけるネックリンカーの役割2010

    • Author(s)
      磯島広
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      アスティとくしま
    • Year and Date
      2010-11-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] キネシン1モータードメインのヌクレオチドフリー状態での結晶構造解析2010

    • Author(s)
      牧野司
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      アスティとくしま
    • Year and Date
      2010-10-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Structural basis for the coordinated movement of kinesin motor protein2010

    • Author(s)
      Michio Tomishige
    • Organizer
      1st HSFP AlumniMeeting - Japan
    • Place of Presentation
      Yayoi Auditorium-Annex, Univ. of Tokyo
    • Year and Date
      2010-10-09
    • Related Report
      2011 Final Research Report
  • [Presentation] Structural basis for the coordinated movement of kinesin motor protein2010

    • Author(s)
      Michio Tomishige
    • Organizer
      1st HSFP Alumni Meeting-Japan
    • Place of Presentation
      Yayoi Auditorium-Anne, Univ.of Tokyo
    • Year and Date
      2010-10-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] ジスルフィド架橋法を用いたキネシン1のネックリンカーのストレインセンサーとしての役割の研究2010

    • Author(s)
      新谷大和
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学川内キャンパス
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] キネシン1の協調的な二足歩行運動を可能にする構造基盤の解析2010

    • Author(s)
      牧野司
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学川内キャンパス
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] キネシン1が選択的に前方にステップする仕組み2010

    • Author(s)
      磯島広
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学川内キャンパス
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 結晶構造解析と一分子計測法の融合により明らかになったモータータンパク質キネシンの二足歩行メカニズム2010

    • Author(s)
      牧野司
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2010-06-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] キネシン頭部の選択的な前方着地におけるネックリンカーの役割2010

    • Author(s)
      磯島広
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2010-06-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Structural basis for the coordinated processive movement of kinesin, EMBO Conference Series2010

    • Author(s)
      Michio Tomishige
    • Organizer
      Microtubules: Structure, Regulation and Functions
    • Place of Presentation
      EMBL Advanced Training Centre, Heidelberg, Germany
    • Year and Date
      2010-06-05
    • Related Report
      2011 Final Research Report
  • [Presentation] Microtubules : Structure, Regulation and Functions2010

    • Author(s)
      Michio Tomishige
    • Organizer
      Structural basis for the coordinated processive movement of kinesin EMBO Conference Series "Microtubules : Structure, Regulation and Functions
    • Place of Presentation
      EMBL Advanced Training Centre, Heidelberg, Germany
    • Year and Date
      2010-06-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] キネシン頭部の選択的な前方着地におけるネックリンカーの役割2010

    • Author(s)
      磯島広
    • Organizer
      第10回東京大学生命科学シンポジウム
    • Place of Presentation
      東京大学本郷キャンパス
    • Year and Date
      2010-05-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] Structural basis for the mechanochemical coupling of kinesin-1 revealed by crystal structural and biochemical analyses2010

    • Author(s)
      Tsukasa Makino
    • Organizer
      54th Annual Meeting of Biophysical Society
    • Place of Presentation
      Moscone Center米国サンフランシスコ
    • Year and Date
      2010-02-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] Optimal size of the neck linker is important for the coordinated nrocessive movement of kinesin-12010

    • Author(s)
      Hiroshi Isojima
    • Organizer
      54th Annual Meeting of Biophysical Society
    • Place of Presentation
      Moscone Center米国サンフランシスコ
    • Year and Date
      2010-02-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] Single molecule observation of structural changes of kinesin motor protein2009

    • Author(s)
      Michio Tomishige
    • Organizer
      International Symposium onWatching Biomolecules in Action
    • Place of Presentation
      ライフサイエンスセンタ ー、大阪
    • Year and Date
      2009-12-15
    • Related Report
      2011 Final Research Report
  • [Presentation] Single molecule observation of structural changes of kinesin motor protein2009

    • Author(s)
      Michio Tomishige
    • Organizer
      International Symposium on Watching Biomolecules in Action
    • Place of Presentation
      ライフサイエンスセンター、大阪
    • Year and Date
      2009-12-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] Role of the neck linker on the preferential forward stepping of kinesin2009

    • Author(s)
      Hiroshi Isojima
    • Organizer
      International Symposium on Watching Biomolecules in Action
    • Place of Presentation
      ライフサイエンスセンター、大阪
    • Year and Date
      2009-12-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] Structural basis for the coordinated processive movement of microtubule-based motor kinesin, International Symposium2009

    • Author(s)
      Michio Tomishige
    • Organizer
      Innovative Nanoscience ofSupermolecular Motor Proteins Working in Biomembranes
    • Place of Presentation
      京都大学芝蘭会館
    • Year and Date
      2009-09-09
    • Related Report
      2011 Final Research Report
  • [Presentation] Structural basis for the coordinated processive movement of microtubule-based motor kinesin2009

    • Author(s)
      Michio Tomishige
    • Organizer
      International Symposium "Innovative Nanoscience of Supermolecular Motor Proteins Working in Biomembranes"
    • Place of Presentation
      京都大学 芝蘭会館
    • Year and Date
      2009-09-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] キネシンの協調的二足歩行メカニズム

    • Author(s)
      富重道雄
    • Organizer
      第1回分子モーター討論会
    • Place of Presentation
      東京大学本郷キャンパス
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Structural basis for the coordinated walking mechanism of kinesin motor protein

    • Author(s)
      Michio Tomishige
    • Organizer
      IMS Symposium on "Recent Development of Spectroscopy and Spatial and Temporal Hierarchical Structures in Molecular Science", Institute for Molecular Science
    • Place of Presentation
      Institute for Molecular Science, Okazaki
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Structural basis for the coordinated processive movement of kinesin motor protein

    • Author(s)
      Michio Tomishige
    • Organizer
      The 17th International Biophysics Congress, Symposium "Cytoskeleton and motor dynamics"
    • Place of Presentation
      Beijing, China
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Strain-dependent regulation of kinesin-1 as studied by disulfide-crosslinking of the neck linker

    • Author(s)
      新谷大和
    • Organizer
      日本生物物理学会第49回年会
    • Place of Presentation
      兵庫県立大学姫路書写キャンパス
    • Related Report
      2011 Annual Research Report
  • [Presentation] Role of the neck linker on the front head gating mechanism of kinesin-1

    • Author(s)
      Xiao Ling
    • Organizer
      The 17th International Biophysics Congress
    • Place of Presentation
      Beijing, China
    • Related Report
      2011 Annual Research Report
  • [Presentation] The neck linker of kinesin-1 functions as a regulator of ATP hydrolysis reaction

    • Author(s)
      Xiao Ling
    • Organizer
      56th Annual Meeting of Biophysical Society
    • Place of Presentation
      San Diego, CA, USA
    • Related Report
      2011 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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