Single molecule FRET study for the walking mechanism of molecular motor kinesin
| Project/Area Number |
21370066
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TOMISHIGE Michio 東京大学, 大学院・工学系研究科, 准教授 (50361530)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2010: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2009: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Keywords | 一分子計測 / 蛍光共鳴エネルギー移動 / 分子機械 / 分子モーター / 構造変化 / 細胞内輸送 / 生物物理 / 一分子計測(SMD) / ナノマシン |
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| Research Abstract |
Molecular motor kinesin walks along microtubules by alternately moving two motor heads. We hypothesized based on atomic structural models that the neck linkers connecting two heads are essential for the coordination between heads. We tested this hypothesis by observing at the single molecule level the movements and structural changes of the mutant kinesins whose tension posed to the neck linker were reduced or abolished and provided the structural basis to explain the walking mechanism of kinesin.
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Report
(4 results)
Research Products
(33 results)
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[Presentation] Microtubules : Structure, Regulation and Functions2010
Author(s)
Michio Tomishige
Organizer
Structural basis for the coordinated processive movement of kinesin EMBO Conference Series "Microtubules : Structure, Regulation and Functions
Place of Presentation
EMBL Advanced Training Centre, Heidelberg, Germany
Year and Date
2010-06-05
Related Report
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