Investigation of the folding dynamics of proteins based on the advanced method of single molecule detection
| Project/Area Number |
21370072
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Research Collaborator |
KAMAGATA Kiyoto 東北大学, 多元物質科学研究所, 助教 (90432492)
OIKAWA Hiroyuki 東北大学, 多元物質科学研究所, 教育研究支援者
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2010: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2009: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Keywords | フォールディング / 蛋白質 / 折り畳み / ダイナミクス / 一分子検出 / タンパク質 / 一分子蛍光観察 / 時系列データ / ラインフォーカス共焦点顕微鏡 / 一分子観察 / タンパク質の折り畳み / 蛍光測定装置 / シトクロムc / β-ラクトグロブリン / マイクロ加工技術 |
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| Research Abstract |
In this project, we first developed spherical mirror system for the detection of fluorescence from single molecules for long periods. Based on the developed system, we successfully determined the energy landscape for cytochrome c. We next developed line conforcal microscopy for the fast time resolved measurements of single molecule fluorescence time series. The method was used to investigate the unfolded state dynamics of the B domain of protein A, and revealed the presence of substates in the unfolded ensemble. Further applications of the developed methods for other protein systems are currently underway.
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Report
(4 results)
Research Products
(60 results)
