Study on the role of Rab33 in membrane trafficking

• Project/Area Number: 21370087
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Tohoku University
• Principal Investigator: FUKUDA Mitsunori 東北大学, 大学院・生命科学研究科, 教授 (50311361)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2009: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: Rab33 / エフェクター / 膜輸送 / Atg16L1 / オートファジー / 低分子量G蛋白質 / オルガネラ / 神経細胞

Research Abstract

Small GTPase Rab33 is mostly conserved in vertebrates and has been suggested to be involved in specific membrane trafficking events, although its precise role remained to be determined. In this study, we identified Atg16L and RUFY2/3 as novel GTP-Rab33-specific binding proteins and demonstrated the possible involvement of Rab33 in the regulation of autophagosomal maturation and neuronal function.

Research Products

• [Journal Article] Atg16L2, a novel isoform of mammalian Atg16L that is not essential for canonical autophagy despite forming an Atg12. 5-16L2 complex. (2011)
  • Author(s): Ishibashi, K., Fujita, N., Kanno, E., Omori, H., Yoshimori, T., Itoh, T. & Fukuda, M.
  • Journal Title: Autophagy Volume: 7 Pages: 1500-1513
  • Peer Reviewed
• [Journal Article] A possible role of Atg8 homologues as a scaffold for signal transduction (2011)
  • Author(s): Itoh, T. & Fukuda, M.
  • Journal Title: Autophagy Volume: 7 Pages: 1080-1081
  • Peer Reviewed
• [Journal Article] Genome-wide investigation of the Rab binding activity of RUN domains : Development of a novel tool that specifically traps GTP-Rab35 (2011)
  • Author(s): Fukuda, M., Kobayashi, H., Ishibashi, K. & Ohbayashi, N.
  • Journal Title: Cell Struct. Funct Volume: 36 Pages: 155-170
  • NAID: 40019163999
  • Peer Reviewed
• [Journal Article] OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation (2011)
  • Author(s): Itoh, T., Kanno, E., Uemura, T., Waguri, S. & Fukuda, M.
  • Journal Title: J. Cell Biol Volume: 192 Pages: 839-853
  • Peer Reviewed
• [Journal Article] TBC proteins : GAPs for mammalian small GTPase Rab? (2011)
  • Author(s): Fukuda, M.
  • Journal Title: Biosci. Rep Volume: 31 Pages: 159-168
  • Peer Reviewed
• [Journal Article] OATLI, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation (2011)
  • Author(s): Itoh, T., Kanno, E., Uemura, T., Waguri, S. & Fukuda, M
  • Journal Title: J. Cell Boil Volume: 192 Issue: 5 Pages: 839-853
  • DOI: 10.1083/jcb.201008107
  • Peer Reviewed
• [Journal Article] Genome-wide Investigation of the Rab Binding Activity of RUN Domains: Development of a Novel Tool that Specifically Traps GTP-Rab35 (2011)
  • Author(s): Fukuda, M., Kobayashi, H., Ishibashi, K., Ohbayashi, N.
  • Journal Title: Cell Structure and Function Volume: 36 Issue: 2 Pages: 155-170
  • DOI: 10.1247/csf.11001
  • NAID: 130004053880
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed
• [Journal Article] Atg16L2, a novel isoform of mammalian Atg16L that is not essential for canonical autophagy despite forming an At12-5-16L2 complex (2011)
  • Author(s): Ishibashi, K., Fujita, N., Kanno, E., Omori, H.,Yoshimori, T., Itoh, T .& Fukuda. M
  • Journal Title: Autophagy Volume: 7 Issue: 12 Pages: 1500-1513
  • DOI: 10.4161/auto.7.12.18025
  • Peer Reviewed
• [Journal Article] TBC proteins : GAPs for mammalian small GTPase Rab? (2011)
  • Author(s): Fukuda, M.
  • Journal Title: Biosci.Rep. Volume: 31 Pages: 159-168
  • Peer Reviewed
• [Journal Article] Comprehensive screening for novel Rab-binding proteins by GST pull-down assay using 60 different mammalian Rabs (2010)
  • Author(s): Kanno, E., Ishibashi, K., Kobayashi, H., Matsui, T., Ohbayashi, N. & Fukuda, M.
  • Journal Title: Traffic Volume: 11 Pages: 491-507
  • Peer Reviewed
• [Journal Article] Role of the polybasic sequence in the Doc2β C2B domain in dense-core vesicle exocvtosis in PC12 cells. (2010)
  • Author(s): Sato, M., Mori, Y., Matsui, T., Aoki, R., Oya, M., Yanagihara, Y., Fukuda, M., Tsuboi, T.
  • Journal Title: J.Neurochem. Volume: 114 Pages: 171-181
  • Peer Reviewed
• [Journal Article] Phosphorylation of AATYK1 by Cdk5 suppresses its tyrosine phosphorylation. (2010)
  • Author(s): Tsutsumi, K., Takano, T., Endo, R., Fukuda, M., Ohshima, T., Tomomura, M., Hisanaga, S.
  • Journal Title: PLoS One Volume: 5
  • Peer Reviewed
• [Journal Article] Storage of GABA in chromaffin granules and not in synaptic-like microvesicles in rat adrenal medullary cells. (2010)
  • Author(s): Harada, K., Matsuoka, H., Nakamura, J., Fukuda, M., Inoue, M.
  • Journal Title: J.Neurochem. Volume: 114 Pages: 617-626
  • Peer Reviewed
• [Journal Article] AATYK1A phosphorylation by Cdk5 regulates the recycling endosome pathway. (2010)
  • Author(s): Takano, T., Tsutsumi, K., Saito, T., Asada, A., Tomomura, M., Fukuda, M., Hisanaga, S.
  • Journal Title: Genes Cells Volume: 15 Pages: 783-797
  • Peer Reviewed
• [Journal Article] A novel 65kDa RNA-binding protein in squid presynaptic terminals. (2010)
  • Author(s): Lico, D.T.P., Rosa, J.C., DiGiorgis, J.A., de Vasconcelos, E.J.R., Casaletti, L., et al.
  • Journal Title: Neuroscience 166 Pages: 73-83
  • Peer Reviewed
• [Journal Article] Redistribution of Rab27-specific effector Slac2-c, but not Slp4-a, after isoproterenol-stimulation in rat parotid acinar cells. (2009)
  • Author(s): Imai, A., Fukuda, M., Yoshie, S., Nashida, T., Shimomura, H.
  • Journal Title: Arch.Oral Biol. 54 Pages: 361-368
  • Peer Reviewed
• [Journal Article] Redistribution of small GTP-binding protein, Rab27B, in rat parotid acinar cells after stimulation with isoproterenol. (2009)
  • Author(s): Imai, A., Yoshie, S., Nashida, T., Fukuda, M., Shimomura, H.
  • Journal Title: Eur.J.Oral Sci. 117 Pages: 224-230
  • Peer Reviewed
• [Journal Article] Anterograde transport of TrkB in axons is mediated by direct interaction with Slp1 and Rab27. (2009)
  • Author(s): Arimura, N., Kimura, T., Nakamuta, S., Taya, S., Funahashi, Y., Hattori, A., et al.
  • Journal Title: Dev.Cell 16 Pages: 675-686
  • Peer Reviewed
• [Journal Article] Regulation of the intracellular distribution, cell surface expression, and protein levels of AMPA receptor GluR2 subunits by the monocarboxylate transporter MCT2 in neuronal cells. (2009)
  • Author(s): Maekawa, F., Tsuboi, T., Fukuda, M., Pellerin, L.
  • Journal Title: J.Neurochem. 109 Pages: 1767-1778
  • Peer Reviewed
• [Journal Article] A coated vesicle-associated kinase of 104kDa(CVAK104)induces lysosomal degradation of frizzled 5(Fzd5). (2009)
  • Author(s): Terabayashi, T., Funato, Y., Fukuda, M., Miki, H.
  • Journal Title: J.Biol.Chem. 284 Pages: 26716-26724
  • Peer Reviewed
• [Presentation] Rabの網羅的解析ツールを用いた新規膜輸送経路の探索と新たなRab機能の同定 (2011)
  • Author(s): 福田光則
  • Organizer: 第84回日本生化学会大会シンポジウム「メンブレントラフィック研究の最近のトレンド(低分子量GTPaseによる多彩な制御機構)」
  • Place of Presentation: 京都
  • Year and Date: 2011-09-24
• [Presentation] Rabの網羅的解析ツールを用いた新規膜輸送経路の探索と新たなRab機能の同定 (2011)
  • Author(s): 福田光則
  • Organizer: 第84回日本生化学会大会シンポジウム「メンブレントラフィック研究の最近のトレンド」
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2011-09-24
• [Presentation] オートファジーに関与するRab-GAPの探索 (2010)
  • Author(s): 伊藤敬、菅野栄子、植村武文、和栗聡、福田光則
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Rab36はダイニン・ダイナクチン複合体依存的にリソソームの核周辺への凝集を引き起こす (2010)
  • Author(s): 松井貴英、大林典彦、丸田優人、伊藤敬、福田光則
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-21
• [Presentation] オートファジー制御に関与する低分子量GTPase Rabの機能解析 (2009)
  • Author(s): 福田光則
  • Organizer: 第82回日本生化学会大会シンポジウム「低分子量GTPaseから眺めるメンブレントラフィック研究の新展開」
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] オートファジー制御に関与する低分子量GTPase Rabの機能解析 (2009)
  • Author(s): 福田光則
  • Organizer: 第82回日本生化学会大会シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] Atg16L1 is a potential effector for small GTPase Rab33 that modulates autophagosome formation. (2009)
  • Author(s): 福田光則
  • Organizer: 5th International Symposium on Autophagy
  • Place of Presentation: Otsu
  • Year and Date: 2009-09-25
• [Remarks]
  • URL: http://www.lifesci.tohoku.ac.jp/research/field_list/membrane_trafficking/t_fukuda
• [Remarks]
  • URL: http://www.lifesci.tohoku.ac.jp/research/field_list/membrane_trafficking/t_fukuda
• [Remarks]
  • URL: http://www.lifesci.tohoku.ac.jp/teacher/neuro/t_fukuda.html