Studies on the molecular mechanisms of induction and suppression of RNA silencing in plants

• Project/Area Number: 21380034
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: MESHI Tetsuo 独立行政法人農業生物資源研究所, 植物科学研究領域, 領域長 (40157813)
• Co-Investigator(Kenkyū-buntansha): ISHIKAWA Masayuki 独立行政法人農業生物資源研究所, 植物科学研究領域・植物-微生物間相互作用研究ユニット, ユニット長 (70192482)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000); Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
• Keywords: RNAサイレンシング / 植物 / ウイルス / サイレンシングサプレッサー

Research Abstract

RNA silencing is one of major antiviral responses in plants. In RNA silencing, RNA-induced silencing complexes(RISCs) recognize target mRNAs to inhibit the expression of corresponding proteins. Here, we found that AGO1 protein binds double-stranded small interfering RNAs(siRNAs) with the help of a molecular chaperone HSP90 and its co-chaperone CYP40, and then, one of the siRNA strands is removed to form an active RISC.

Research Products

• [Journal Article] Cyclophilin 40 Facilitates HSP90-mediated RISC Assembly in Plants (2012)
  • Author(s): Taichiro Iki, Manabu Yoshikawa, Tetsuo Meshi, and Masayuki Ishikawa
  • Journal Title: EMBO J Volume: 31(2) Pages: 267-278
  • Peer Reviewed
• [Journal Article] Cyclophilin 40 facilitates HSP90-mediated RISC assembly in plants (2012)
  • Author(s): Iki, et al
  • Journal Title: EMBO J Volume: 31 Issue: 2 Pages: 267-278
  • DOI: 10.1038/emboj.2011.395
  • Peer Reviewed
• [Journal Article] In vitro assembly of plant RNA-induced silencing complexes facilitated by molecular chaperone HSP90 (2010)
  • Author(s): Taichiro Iki, Manabu Yoshikawa, Masaki Nishikiori, Mauren C. Jaudal, Eiko Matsumoto-Yokoyama, Ichiro Mitsuhara, Tetsuo Meshi and Masayuki Ishikawa
  • Journal Title: Mol. Cell Volume: 39(2) Pages: 282-291
  • Peer Reviewed
• [Journal Article] Inducible viral inoculation system with cultured plant cells facilitates a biochemical approach for virus-induced RNA silencing (2010)
  • Author(s): Atsushi Tamai, Koji Dohi, Masashi Mori, Tetsuo Meshi and Masayuki Ishikawa
  • Journal Title: Arch. Virol Volume: 155(3) Pages: 297-303
  • Peer Reviewed
• [Journal Article] In vitro assembly of plant RNA-induced silencing complexes facilitated by molecular chaperone HSP90 (2010)
  • Author(s): Iki T, Meshi T, Ishikawa M, 他5名
  • Journal Title: Molecular Cell Volume: Vol.39 Pages: 282-291
  • Peer Reviewed
• [Journal Article] 植物における無細胞RISC形成系の確立と分子シャペロンHSP90によるRISC形成の促進 (2010)
  • Author(s): 井木太一郎, 石川雅之
  • Journal Title: ライフサイエンス新着論文レビュー Volume: (オンライン)
• [Presentation] Involvement of HSP90 and the cochaperones on plant RISC assembly (2011)
  • Author(s): Taichiro Iki, Manabu Yoshikawa, Tetsuo Meshi, and Masayuki Ishikawa
  • Organizer: The 16th Annual Meeting of the RNA Society and the 13th Annual Meeting of the RNA Society of Japan
  • Place of Presentation: 京都
  • Year and Date: 2011-06-15
• [Presentation] Involvement of HSP90 and the cochaperones on plant RISC assembly (2011)
  • Author(s): Iki, et al
  • Organizer: The 16th Annual Meeting of the RNA Society and The 13th Annual Meeting of the RNA Society of Japan
  • Place of Presentation: 京都国際会議場(京都府)
  • Year and Date: 2011-06-15
• [Presentation] 植物RISC形成におけるHSP90の役割(招待講演) (2010)
  • Author(s): 井木太一郎,吉川学,石川雅之
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] 植物RISC形成におけるHSP90の役割 (2010)
  • Author(s): 井木太一郎, 吉川学, 石川雅之
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸(招待講演)
  • Year and Date: 2010-12-08
• [Presentation] In vitro assembly of plant RNA-induced silencing complex (2010)
  • Author(s): Taichiro Iki, Manabu Yoshikawa, Masaki Nishikiori, Ichiro Mitsuhara, Tetsuo Meshi, Masayuki Ishikawa
  • Organizer: EMBO Workshop 2010 : Genomic approaches to interactions between plant viruses their hosts and their vectors
  • Place of Presentation: Fenestrelle(イタリア)
  • Year and Date: 2010-06-13
• [Presentation] In vitro assembly of plant RNA-induced silencing complex (2010)
  • Author(s): Iki T, Yoshikawa M, Nishikiori M, Mitsuhara I, Meshi T, Ishikawa M
  • Organizer: EMBO Workshop 2010 : Genomic approaches to interactions between plant viruses their hosts and their vectors
  • Place of Presentation: イタリア、Fenestrelle(ポスター発表)
  • Year and Date: 2010-06-13
• [Presentation] 植物におけるRNA-induced silencing complexの無細胞形成系の確立 (2010)
  • Author(s): 井木太一郎、吉川学、ジャウダルモーレン、横山英子、錦織雅樹、光原一朗、飯哲夫、石川雅之
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-21
• [Presentation] 植物におけるRNA-induced silencing complexの無細胞形成系の確立 (2010)
  • Author(s): 井木太一郎、飯哲夫、石川雅之, 他5名
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-21
• [Book] 植物における無細胞RISC形成系の確立と分子シャペロン. HSP90によるRISC形成の促進
  • Author(s): 井木太一郎、石川雅之
  • Publisher: ライフサイエンス新着論文レビュー(オンライン)