Project/Area Number |
21380036
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied entomology
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Research Institution | Kyushu University |
Principal Investigator |
BANNO Yutaka 九州大学, (連合)農学研究科(研究院), 准教授 (50192711)
|
Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Kimiko 独立行政法人農業生物資源研究所, ユニット長 (40370689)
YOKOYAMA Takeshi 東京農工大学, 大学院・農学研究科, 准教授 (20210635)
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Project Period (FY) |
2009-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
|
Keywords | カイコ / ミュータジェネシス / 突然変異 / 遺伝子資源 / ランダムミュータジェネシス / クワコ / 凍結保存 / SNPsマーカー / 倍数体 / SNPSマーカー / 単為発生 / ポストゲノム時代 / 突然変異体 / 遺伝資源 / SNPs / 化学変異源 |
Research Abstract |
To develop random mutagenesis system in the silkworm, the possibility of mutagenesis by ethyl nitrosourea (ENU) was investigated. ENU was proved to be a useful chemical source as a result, . The fertilized eggs were immersed in ENU solution. The phenotypes of 798 individuals treated with ENU were evaluated at various stages. Abnormal characters were discovered in 212 individuals of them. To survey the mutant, we selected 40 pairs from sib-crossing of individuals having abnormal characters. In G2 or G3 generation, more than 20 mutants were discovered as mutant candidate. In addition, we developed one method to obtain triploid silkworm with high ratio by using CO2 gas. All of the sperm of triploid silkworm were apyrene sperm that can assist fertilization of eupyrene sperm. Moreover, we performed linkage analysis of mutants with SNPs information and marker gene. We also found new characteristics in wild silkworm, Bombyx mandarina: black pupa and big egg.
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Report
(6 results)
Research Products
(16 results)
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[Presentation] 炭酸水処理による倍数体蚕の誘発2013
Author(s)
伊藤加津沙, 青木花織, 蜷木 理, 竹村洋子, 横山 岳
Organizer
日本蚕糸学会第83回大会
Place of Presentation
農林水産技術会議事務局筑波事務所(つくば市)
Year and Date
2013-03-19
Related Report
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