2013 Fiscal Year Final Research Report
| Project/Area Number |
21380036
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Kyushu University |
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Principal Investigator |
BANNO Yutaka 九州大学, (連合)農学研究科(研究院), 准教授 (50192711)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Kimiko 独立行政法人農業生物資源研究所, ユニット長 (40370689)
YOKOYAMA Takeshi 東京農工大学, 大学院・農学研究科, 准教授 (20210635)
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| Project Period (FY) |
2009-04-01 – 2014-03-31
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| Keywords | カイコ / ミュータジェネシス / 突然変異 / 遺伝子資源 |
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| Research Abstract |
To develop random mutagenesis system in the silkworm, the possibility of mutagenesis by ethyl nitrosourea (ENU) was investigated. ENU was proved to be a useful chemical source as a result, . The fertilized eggs were immersed in ENU solution. The phenotypes of 798 individuals treated with ENU were evaluated at various stages. Abnormal characters were discovered in 212 individuals of them. To survey the mutant, we selected 40 pairs from sib-crossing of individuals having abnormal characters. In G2 or G3 generation, more than 20 mutants were discovered as mutant candidate. In addition, we developed one method to obtain triploid silkworm with high ratio by using CO2 gas. All of the sperm of triploid silkworm were apyrene sperm that can assist fertilization of eupyrene sperm. Moreover, we performed linkage analysis of mutants with SNPs information and marker gene. We also found new characteristics in wild silkworm, Bombyx mandarina: black pupa and big egg.
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