Studies on the origin of exotic loaches and their impact on indigenous species

• Project/Area Number: 21380114
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Hokkaido University
• Principal Investigator: ARAI Katsutoshi 北海道大学, 大学院・水産科学研究院, 教授 (00137902)
• Co-Investigator(Kenkyū-buntansha): MURAKAMI Masaru 麻布大学, 獣医学部, 教授 (80271360) ; YABE Mamoru 北海道大学, 大学院・水産科学研究院, 教授 (80174572)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 倍数体 / クローン / ミトコンドリアDNA / 反復配列 / マイクロサテライト / 交雑 / FISH / 生物多様性保全 / RAG1 / IRBP2 / 交雑種 / 異数体 / ミニサテライト / 減数分裂

Research Abstract

Loaches collected in Japan and adjacent area were genetically characterized by chromosome observation, genome size estimation and sequence analyses of mitochondrial and nuclear DNA markers. As a result, Japanese market samples were genetically identical or very similar to those distributed in China. As artificial inter-specific hybrids between indigenous loach and exotic mud loach species exhibit fertility in females, genetic contamination is worried in Japanese wild populations.

Research Products

• [Journal Article] Induction of viable gynogenetic progeny using eggs and UV irradiated sperm from the Chinese tetraploid loach, Misgurnus anguillicaudatus (2012)
  • Author(s): Ya-Juan Li, Zhuo Yu, Ming-Zhao Zhang, Cong Qian, Syuiti Abe, Katsutoshi Arai
  • Journal Title: Aquaculture International Volume: (印刷中)
  • NAID: 120005300017
  • Peer Reviewed
• [Journal Article] Diploid clone produces unreduced diploid gametes but tetraploid clone generates reduced diploid gametes in the Misgurnus loach (2012)
  • Author(s): Kagayaki Morishima, Hiroyuki Yoshikawa, Katsutoshi Arai
  • Journal Title: Biology of Reproduction Volume: 86巻33 Issue: 2 Pages: 1-7
  • DOI: 10.1095/biolreprod.111.093302
  • Peer Reviewed
• [Journal Article] Cold-shock eliminates female nucleus in fertilized eggs to induce androgenesis in the loach (Misgurnus anguillicaudatus), a teleost fish (2011)
  • Author(s): Kagayaki Morishima, Takafumi Fujimoto, Mami Sato, Ayako Kawae, Yan Zhao, Etsuro Yamaha, Katsutoshi Arai
  • Journal Title: BMC Biotechnology Volume: 11巻 Issue: 1 Pages: 116-116
  • DOI: 10.1186/1472-6750-11-116
  • NAID: 120003702855
  • Peer Reviewed
• [Journal Article] Production of loach (Misgurnus anguillicaudatus) germ-line chimera using transplantation of primordial germ cells isolated from cryopreserved blastomeres (2011)
  • Author(s): George S Yasui, Takafumi Fujimoto, Suzu Sakao, Etsuro Yamaha, Katsutoshi Arai
  • Journal Title: Journal of Animal Science Volume: 89巻 Issue: 2 Pages: 2080-2388
  • DOI: 10.2527/jas.2011-4329
  • Peer Reviewed
• [Journal Article] The origin of natural tetraploid loach Misgurnus anguillicaudatus (Teleostei : Cobitidae) inferred from meiotic chromosome configurations (2011)
  • Author(s): Ya-Juan Li, Zhuo Yu, Ming-Zhao Zhang, Cong Qian, Syuiti Abe, Katsutoshi Arai
  • Journal Title: Genetica Volume: 139巻 Issue: 6 Pages: 805-800
  • DOI: 10.1007/s10709-011-9585-x
  • NAID: 120005946682
  • Peer Reviewed
• [Journal Article] Chromosome banding and FISH with rDNA probe in the diploid and tetraploid loach Misgurnus anguillicaudatus (2010)
  • Author(s): Ya-Juan Li, Yi Tian, Ming-Zhao Zhang, Ping-Ping Tian, Zhuo Yu, Syuiti Abe, Katsutoshi Arai
  • Journal Title: Ichthyological Research Volume: 57巻 Issue: 4 Pages: 358-366
  • DOI: 10.1007/s10228-010-0168-0
  • NAID: 10027836748
  • Peer Reviewed
• [Journal Article] Restoration of the loach Misgurnus anguillicaudatus from cryopreserved diploid sperm and induced androgenesis (2010)
  • Author(s): George S Yasui, Takafumi Fujimoto, Katsutoshi Arai
  • Journal Title: Aquaculture Volume: 308巻 Pages: S140-S144
  • DOI: 10.1016/j.aquaculture.2010.05.041
  • NAID: 120002564622
  • Peer Reviewed
• [Journal Article] Chromosome banding and FISH with rDNA probe in the diploid and tetraploid loach Misgurnus anguillicaudatus (2010)
  • Author(s): Li YJ, Tian Y, Zhang MZ, Tian PP, Yu Z, Abe S, Arai K
  • Journal Title: Ichthyological Research Volume: 57 Pages: 358-366
  • NAID: 10027836748
  • Peer Reviewed
• [Presentation] 市販外来ドジョウの倍数性変異とDNAマーカーから見た起源 (2012)
  • Author(s): 趙岩・戸田昌邦・麻生真実・山田綾・荒井克俊(発表者)
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京都)
  • Year and Date: 2012-03-27
• [Presentation] 核DNAから見たクローンドジョウの識別法 (2011)
  • Author(s): 山田綾(発表者)・神門幸大・村上賢・荒井克俊
  • Organizer: 平成23年度日本水産学会北海道支部大会
  • Place of Presentation: 北海道大学(函館市)
  • Year and Date: 2011-11-16
• [Presentation] RAG1遺伝子から見た自然クローンドジョウの雑種起源 (2011)
  • Author(s): 山田綾(発表者)・神門幸大・村上賢・荒井克俊
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎大学(長崎市)
  • Year and Date: 2011-09-30
• [Presentation] Motility, morphology, mitochondria and ATP content of diploid spermatozoa from sex reversed clonal diploid and neo-tetraploid loach, Misgurnus anguillicaudatus (2011)
  • Author(s): Yan Zhao (発表者), Martin Psenicka, Takafumi Fujimoto, Taiju Saito, George S Yasui, Etsuro Yamaha, Katsutoshi Arai
  • Organizer: 3^<rd> International Workshop on the Biology of Fish Gametes
  • Place of Presentation: ブダペスト(ハンガリー)
  • Year and Date: 2011-09-08
• [Presentation] Motility, morphology, mitochondria and ATP content of diploid spermatozoa from sex-reversed clonal diploid and neo-tetraploid loach, Misgurnus anguillicaudatus (2011)
  • Author(s): Zhao Y, Psenicka M, Fujimoto T, Saito T, Yasui GS, Yamaha E, Arai K
  • Organizer: 3rd International Workshop on the Biology of Fish Gametes
  • Place of Presentation: Danubius Hotel Helia (Hungary)
  • Year and Date: 2011-09-08
• [Presentation] Fertility and ploidy of gametes of diploid, triploid and tetraploid loaches, Misgurnus anguillicaudatus, in China (2011)
  • Author(s): Ya-Juan Li, Ming-Zhao Zhang, Cong Qian, Min Gao, Katsutoshi Arai (発表者)
  • Organizer: 3^<rd> International Workshop on the Biology of Fish Gametes
  • Place of Presentation: ブダペスト(ハンガリー)
  • Year and Date: 2011-09-07
• [Presentation] Fertility and ploidy of gametes of diploid, triploid and tetraploid loaches, Misgurnus anguillicaudatus, in China (2011)
  • Author(s): Arai K, Li YJ, Zhang MZ, Qian C, Gao M
  • Organizer: 3rd International Workshop on the Biology of Fish Gametes
  • Place of Presentation: Danubius Hotel Helia (Hungary)
  • Year and Date: 2011-09-07
• [Presentation] Induction of viable gynogenetic progeny using eggs and UV irradiated sperm of Chinese tetraploid loach, Misgurnus anguillicaudatus (2011)
  • Author(s): Ya-Juan Li, Zhuo Yu, Ming-Zhao Zhang, Cong Qian, Syuiti Abe, Katsutoshi Arai (発表者)
  • Organizer: International Workshop, Diversification in Inland Finfish Aquaculture
  • Place of Presentation: ピーセク(チェコ)
  • Year and Date: 2011-05-17
• [Presentation] Induction of viable gynogenetic progeny using eggs and UV irradiated sperm of Chinese tetraploid loach, Misgurnus anguillicaudatus (2011)
  • Author(s): Li YJ, Yu Z. Zhang MZ, Qian C, Abe S, Arai K
  • Organizer: International Workshop, Diversification in Inland Finfish Aquaculture
  • Place of Presentation: Pisek Malt-House (Czech Republic)
  • Year and Date: 2011-05-16
• [Presentation] ドジョウ雌×カラドジョウ雄F1雑種の妊性と配偶子の倍数性 (2011)
  • Author(s): 光山成美・横山幸奈・藤本貴史・荒井克俊(発表者)
  • Organizer: 平成23年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京都)
  • Year and Date: 2011-03-29
• [Presentation] ドジョウ特異的なDNAマーカーの探索Part 2 (2011)
  • Author(s): 神門幸大・堀口裕生・山田綾・藤本貴史・荒井克俊・村上賢
  • Organizer: 平成23年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京都)
  • Year and Date: 2011-03-29
• [Presentation] Genetic and cytogenetic characteristics of natural tetraploid loach Misgurnus anguillicaudatus in China (2010)
  • Author(s): Katsutoshi Arai (発表者)
  • Organizer: The International Meeting on the Genetics of Polyploids
  • Place of Presentation: University of Lisbon, Lisbon, Portgal
  • Year and Date: 2010-11-11
• [Presentation] Genetic and cytogenetic characteristics of natural tetraploid loach Misgurnus anguillicaudatus in China (2010)
  • Author(s): Arai K
  • Organizer: The International Meeting on the Genetics of Polyploids
  • Place of Presentation: リスボン大学、リスボン市、ポルトガル 招待講演
  • Year and Date: 2010-11-11
• [Presentation] 中国産二倍体、三倍体および四倍体ドジョウ由来子孫の発生能力と染色体構成 (2010)
  • Author(s): 李雅娟・張明昭・周賀・荒井克俊(発表者)
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都大学(京都市)
  • Year and Date: 2010-09-24
• [Presentation] ジョウ特異的なDNAマーカーの探索 (2010)
  • Author(s): 神門幸大・堀口裕生・山田綾・藤本貴史・荒井克俊・村上賢
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都大学(京都市)
  • Year and Date: 2010-09-23
• [Presentation] 人為雌性発生子孫の発生能力と核型から推定した中国産自然四倍体ドジョウのゲノム構成 (2010)
  • Author(s): 李雅娟・于卓・銭聰・荒井克俊(発表者)
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都大学(京都市)
  • Year and Date: 2010-09-22
• [Presentation] ドジョウ特異的なDNAマーカーの探索 (2010)
  • Author(s): 村上賢
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都大学(京都市)
  • Year and Date: 2010-09-22
• [Presentation] 中国産自然四倍体ドジョウ染色体のFISHと分染 (2010)
  • Author(s): 李雅娟・張明昭・魏傑・阿部周一・荒井克俊(発表者)
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 日本大学(藤沢市)
  • Year and Date: 2010-03-29
• [Presentation] 中国産自然四倍体ドジョウ染色体のFISHと分染 (2010)
  • Author(s): 李雅娟, 張明昭, 魏傑, 阿部周一, 荒井克俊
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 藤沢市、日本大学
  • Year and Date: 2010-03-29
• [Presentation] 中国産四倍体ドジョウの減数分裂における染色体の挙動 (2010)
  • Author(s): 李雅娟・于卓・荒井克俊(発表者)
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 日本大学(藤沢市)
  • Year and Date: 2010-03-27
• [Presentation] 中国産四倍体ドジョウの減数分裂における相同染色体の挙動 (2010)
  • Author(s): 李雅娟, 于卓, 荒井克俊
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 藤沢市、日本大学
  • Year and Date: 2010-03-27