Nobel method to transform plants through epigenetic modifications by the cucumber mosaic virus-based vector

• Project/Area Number: 21380203
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Hokkaido University
• Principal Investigator: MASUTA Chikara 北海道大学, 大学院・農学研究院, 教授 (60281854)
• Co-Investigator(Kenkyū-buntansha): KANAZAWA Akira 北海道大学, 大学院・農学研究院, 准教授 (30281794)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2009: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 遺伝子 / 染色体工学 / キュウリモザイクウイルス / エピジェネティクス / メチル化 / TGS / ChIP / CMV / サイレンシング / エピジェネティック

Research Abstract

Gene silencing through transcriptional repression can be induced by targeting double-stranded RNA (dsRNA) to a gene promoter. We show that heritable gene silencing can be induced by targeting dsRNA to the endogenous gene promoters in petunia and tomato plants, using the Cucumber mosaic virus (CMV)-based vector. This CMV-based gene silencing system provides a useful tool to artificially modify DNA methylation in plant genomes and elucidate the mechanism for epigenetic controls.

Research Products

• [Journal Article] The 2b proteins of Cucumber mosaic virus generally have the potential to differentially induce necrosis on Arabidopsis (2012)
  • Author(s): Masuta, C., Inaba, J. and Shimura, H.
  • Journal Title: Plant Signaling & Behavior Volume: 7 Pages: 43-45
  • Peer Reviewed
• [Journal Article] Decrease in Globodera rostochiensis (Woll.) (Nematode:Heteroderidae) population density on resistant tomato cultivars in greenhouse trials (2011)
  • Author(s): Uehara, T., Itou, K., Narabu, T. and Masuta, C.
  • Journal Title: Nematological Research Volume: 41 Pages: 41-44
  • Peer Reviewed
• [Journal Article] Tobacco salicylic acid glucosyltransferase is active toward tuberonic acid (12-hydroxyjasmonic acid) and is induced by mechanical wounding stress (2011)
  • Author(s): Seto, Y., Hamada, S., Ito, H., Masuta, C., Matsui, H., Nabeta, K. and Matsuura, H.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75 Pages: 2316-2320
  • NAID: 10030397521
  • Peer Reviewed
• [Journal Article] Variability in the P1 gene helps to refine phylogenetic relations among Leek yellow stripe virus isolates from garlic (2011)
  • Author(s): Yoshida, N, Shimura, H., Yamashita, K., Suzuki, M. and Masuta, C.
  • Journal Title: Archives of Virology Volume: 157 Pages: 147-153
  • Peer Reviewed
• [Journal Article] Infection dynamics in viral spread and interference under the synergism between Cucumber mosaic virus and Turnip mosaic virus (2011)
  • Author(s): Takeshita, M., Koizumi, E., Noguchi, M., Sueda, K., Shimura, H., Ishikawa, N., Matsuura, H, Ohshima, K., Natsuaki, T., Kuwata, S., Furuya, N., Tsuchiya, K. and Masuta, C.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 25 Pages: 18-27
  • Peer Reviewed
• [Journal Article] Virus-induced necrosis Is a consequence of direct protein-protein interaction between a viral RNA-silencing suppressor and a host catalase (2011)
  • Author(s): Inaba, J., Kim, B. M., Shimura, H. and Masuta, C.
  • Journal Title: Plant Physiology Volume: 156 Pages: 2026-2036
  • Peer Reviewed
• [Journal Article] Size and positional effects of promoter RNA segments on virus-induced RNA-directed DNA methylation and transcriptional genesilencing (2011)
  • Author(s): Otagaki, S., Kawai, M., Masuta, C. and Kanazawa, A.
  • Journal Title: Epigenetics Volume: 6 Pages: 681-691
  • Peer Reviewed
• [Journal Article] Combinations of a host resistance gene and the CI gene of Turnip mosaic virus differentially regulate sympotom expression in Brassica rapa cultivars (2011)
  • Author(s): Fujiwara, A., Inukai, T., Kim, B. M. and Masuta, C.
  • Journal Title: Archives of Virology Volume: 156 Pages: 1575-1581
  • Peer Reviewed
• [Journal Article] RNA-mediated epigenetic modifications of an endogenous gene targeted by a viral vector:a potent gene silencing system to produce a plant that does not carry atransgene but has altered traits (2011)
  • Author(s): Kanazawa, A., Inaba, J., Kasai, M., Shimura, H. and Masuta, C.
  • Journal Title: Plant Signaling & Behavior Volume: 6 Pages: 1090-1093
  • Peer Reviewed
• [Journal Article] A viral satellite RNA induces yellow symptoms on tobacco by targeting a gene involved in chlorophyll biosynthesis using the RNA silencing machinery (2011)
  • Author(s): Shimura, H., Pantaleo, V., Ishihara, T., Myojo, N., Inaba, J., Sueda, K., Burgyan, J. and Masuta, C.
  • Journal Title: PLoS Pathogens Volume: 7(5)
  • NAID: 120003059662
  • Peer Reviewed
• [Journal Article] Distal transport of exogenously applied jasmonoyl-isoleucine with wounding stress (2011)
  • Author(s): Sato, C., Aikawa, K., Sugiyama, S., Nabeta, K., Masuta, C. and Matsuura, H.
  • Journal Title: Plant Cell Physioogy Volume: 52 Pages: 509-517
  • NAID: 10029709585
  • Peer Reviewed
• [Journal Article] Multigenic system controlling viral systemic infection determined by the interactions between Cucumber mosaic virus genes and QTLs of soybean cultivar (2011)
  • Author(s): Ohnishi, S., Echizenya, I., Yoshimoto, Kim, B. M., E., Inukai, T. and Masuta, C.
  • Journal Title: Phytopathology Volume: 101 Pages: 575-582
  • Peer Reviewed
• [Journal Article] Virus induced gene silencing in Antirrhinum majus using the Cucumber mosaic virus vector : Functional analysis of the AINTEGUMENTA (Am-ANT) gene of A. majus (2011)
  • Author(s): Kim, B. M., Inaba, J. and Masuta, C.
  • Journal Title: Horticulture, Environment, and Biotechnology Volume: 52 Pages: 176-182
  • NAID: 120003918919
  • Peer Reviewed
• [Journal Article] Virus induced gene silencing in Antirrhinum majus using the Cucumber mosaic virus vector : Functional analysis of the AINTEGUMENTA (AmANT) gene of A.majus (2011)
  • Author(s): Kim, B.M.
  • Journal Title: Horticulture, Environment, and Biotechnology Volume: 52 Pages: 176-182
  • Peer Reviewed
• [Journal Article] A viral satellite RNA induces yellow symptoms on tobacco by targeting a gene involved in chlorophyll biosynthesis using the RNA silencing machinery (2011)
  • Author(s): Shimura, H.
  • Journal Title: PLoS Pathogens Volume: 7(5)
  • Peer Reviewed
• [Journal Article] RNA-mediated epigenetic modifications of an endogenous gene targeted by a viral vector : a potent gene silencing system to produce a plant that does not carry atransgene but has altered traits (2011)
  • Author(s): Kanazawa, A.
  • Journal Title: Plant Signaling & Behavior Volume: 6 Pages: 1090-1093
  • Peer Reviewed
• [Journal Article] Size and positional effects of promoter RNA segments on virus-induced RNA-directed DNA methylation and transcriptional gene silencing (2011)
  • Author(s): Otagaki, S.
  • Journal Title: Epigenetics Volume: 6 Pages: 681-691
  • Peer Reviewed
• [Journal Article] Virus-induced necrosis Is a consequence of direct protein-protein Interaction between a viral RNA-silencing suppressor and a host catalase (2011)
  • Author(s): Inaba, J.
  • Journal Title: Plant Physiology Volume: 156 Pages: 2026-2036
  • Peer Reviewed
• [Journal Article] Virus induced gene silencing in Antirrhinum majus using the Cucumber mosaic virus vector : Functional analysis of the AINTEGUMENTA (Am-ANT) gene of A. majus. (2011)
  • Author(s): Kim, B.M.
  • Journal Title: Horticulture, Environment, and Biotechnology Volume: 52(in press)
  • Peer Reviewed
• [Journal Article] Virus-mediated efficient induction of epigenetic modifications of endogenous genes with phenotypic changes in plants (2010)
  • Author(s): Kanazawa, A., Inaba, J., Shimura, H., Otagaki, S., Tsukahara, S., Matsuzawa, A., Kim, B., Goto, K., Masuta, C.
  • Journal Title: The Plant Journal Volume: 65 Pages: 156-68
  • NAID: 120005946605
  • Peer Reviewed
• [Journal Article] Resistant and Susceptible Responses in Tomato to Cyst Nematode are Differentially Regulated by Salicylic Acid (2010)
  • Author(s): Uehara, T., Sugiyama, S., Matsuura, H., Arie, T. and Masuta, C.
  • Journal Title: Plant Cell Physiology Volume: 52 Pages: 1524-1536
  • NAID: 10027352520
  • Peer Reviewed
• [Journal Article] Risk-managed production of bioactive recombinant proteins using a novel plant virus vector with a helper plant to complement viral systemic movement (2010)
  • Author(s): Fukuzawa, N., Ishihara, T., Itchoda, N., Tabayashi, N., Kataoka, C., Masuta, C. and Matsumura, T.
  • Journal Title: Plant Biotechnology Journal Volume: 9 Pages: 38-49
  • Peer Reviewed
• [Journal Article] Soybean stem growth habit gene Dt1 is an orthologue of Arabidopsis TFL1 (2010)
  • Author(s): Liu, B., Watanabe, S., Uchimiya, T., Kong, F., Kanazawa, A., Xia, Z., Nagamatsu, A., Arai, M., Yamada, T., Kitamura, K., Masuta, C., Harada, K. and Abe, J.
  • Journal Title: Plant Physiology Volume: 153 Pages: 198-210
  • Peer Reviewed
• [Journal Article] HC-Pro, a potyvirus RNA silencing suppressor, cancels cycling of Cucumber mosaic virus in Nicotiana benthamiana plants (2010)
  • Author(s): Fukuzawa, N., Itchoda, N., Ishihara, T., Goto, K., Masuta, C. and Matsumura, T.
  • Journal Title: Virus Genes Volume: 40 Pages: 440-446
  • Peer Reviewed
• [Journal Article] The C-terminal residues of the 2b protein of Cucumber mosaic virus are important for efficient expression in Escherichia coli and DNA binding (2010)
  • Author(s): Sueda, K., Shimura, H., Meguro, A., Uchida T., Inaba J., and Masuta, C.
  • Journal Title: FEBS Letters Volume: 584 Pages: 945-950
  • Peer Reviewed
• [Journal Article] A progeny virus from a cucumovirus pseudorecombinant evolved to gain the ability to accumulate its RNA silencing suppressor leading to systemic infection in tobacco (2010)
  • Author(s): Asaoka, R., Shimura, H., Arai, M. and Masuta, C.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 23 Pages: 32-339
  • Peer Reviewed
• [Journal Article] Mixed Infection in Tomato to Ensure Frequent Generation of A Natural Reassortant Between Two Subgroups of Cucumber mosaic virus (2010)
  • Author(s): Maoka, T., Saito-Hayano, Y., Iwasaki, M., Yoshida, K. and Masuta, C.
  • Journal Title: Virus Genes Volume: 40 Pages: 148-150
  • Peer Reviewed
• [Journal Article] The P3 Protein of turnip mosaic virus can alone induce HR-like cell death in Arabidopsis thaliana carrying TuNI (2010)
  • Author(s): Kim, B., Suehiro, N., Natsuaki, T., Inukai, T. and Masuta, C.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 40 Pages: 144-152
  • Peer Reviewed
• [Journal Article] The P3 Protein of turnip mosaic virus can alone induce HR-like cell death in Arabidopsis thaliana carrying TuNI. (2010)
  • Author(s): Kim, B.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 40 Pages: 144-152
  • Peer Reviewed
• [Journal Article] Mixed Infection in Tomato to Ensure Frequent Generation of A Natural Reassortant Between Two Subgroups of Cucumber mosaic virus. (2010)
  • Author(s): Maoka, T.
  • Journal Title: Virus Genes Volume: 40 Pages: 148-150
  • Peer Reviewed
• [Journal Article] A progeny virus from a cucumovirus pseudorecombinant evolved to gain the ability to accumulate its RNA silencing suppressor leading to systemic infection in tobacco. (2010)
  • Author(s): Asaoka, R.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 23 Pages: 32-339
  • Peer Reviewed
• [Journal Article] The C-terminal residues of the 2b protein of Cucumber mosaic virus are important for efficient expression in Escherichia coli and DNA binding. (2010)
  • Author(s): Sueda, K.
  • Journal Title: FEBS Letters Volume: 584 Pages: 945-950
  • Peer Reviewed
• [Journal Article] HC-Pro, a pot potyvirus RNA silencing suppressor, cancels cycling of Cucumber mosaic virus in Nicotiana benthamia plants. (2010)
  • Author(s): Fukuzawa, N.
  • Journal Title: Virus Genes Volume: 40 Pages: 440-446
  • Peer Reviewed
• [Journal Article] Soybean stem growth habit gene Dt1 is an orthologue of Arabidopsis TFL1. (2010)
  • Author(s): Liu, B.
  • Journal Title: Plant Physiology Volume: 153 Pages: 198-210
  • Peer Reviewed
• [Journal Article] Risk-managed production of bioactive recombinant proteins using a novel plant virus vector with a helper plant to complement viral systemic movement. (2010)
  • Author(s): Fukuzawa, N.
  • Journal Title: Plant Biotechnology Journal Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Resistant and susceptible responses in tomato to cyst nematode are differentially regulated by salicylic acid. (2010)
  • Author(s): Uehara, T
  • Journal Title: Plant Cell Physiology Volume: 51 Pages: 1524-1536
  • Peer Reviewed
• [Journal Article] Virus-mediated efficient induction of epigenetic modifications of endogenous genes with phenotypic changes in plants. (2010)
  • Author(s): Kanazawa, A.
  • Journal Title: The Plant Journal Volume: 65 Pages: 156-168
  • NAID: 120005946605
  • Peer Reviewed
• [Journal Article] The P3 Protein of turnip mosaic virus can alone induce HR-like cell death in Arabidopsis thaliana carrying TuNI. (2010)
  • Author(s): Kim, B.
  • Journal Title: Molecular Plant-Microbe Interactions 40 Pages: 144-152
  • Peer Reviewed
• [Journal Article] Mixed Infection in Tomato to Ensure Frequent Generation of A Natural Reassortant Between Two Subgroups of Cucumber mosaic virus. (2010)
  • Author(s): Maoka, T.
  • Journal Title: Virus Genes 40 Pages: 148-150
  • Peer Reviewed
• [Journal Article] A progeny virus from a cucumovirus pseudorecombinant evolved to gain the ability to accumulate its RNA silencing suppressor leading to systemic infection in tobacco. (2010)
  • Author(s): Asaoka, R.
  • Journal Title: Molecular Plant-Microbe Interactions 23 Pages: 32-339
  • Peer Reviewed
• [Journal Article] The C-terminal residues of the 2b protein of Cucumber mosaic virus are important for efficient expression in Escherichia coli and DNA binding. (2010)
  • Author(s): Sueda, K.
  • Journal Title: FEBS Letters 584 Pages: 945-950
  • Peer Reviewed
• [Journal Article] Purification and cDNA cloning of a wound inducible glucosyltransferase active toward 12-hydroxy jasmonic acid (2009)
  • Author(s): Seto, Y., Hamada, S., Matsuura, H., Matsushige, M., Satou, C., Takahashi, K., Masuta, C., Ito, H., Matsui, H. and Nabeta, K.
  • Journal Title: Phytochemistry Volume: 70 Pages: 370-379
  • Peer Reviewed
• [Journal Article] Simultaneous UPLC MS/MS analysis of endogenous jasmonic acid, salicylic acid, and their related compounds (2009)
  • Author(s): Matsuura, H., Aoi, A., Satou, C., Nakaya, M., Masuta, C. and Nabeta, K.
  • Journal Title: Plant Growth Regulation Volume: 57 Pages: 293-301
  • Peer Reviewed
• [Journal Article] Simultaneous UPLC MS/MS analysis of endogenous jasmonic acid, salicylic acid, and their related compounds. (2009)
  • Author(s): Matuura, H.
  • Journal Title: Plant Growth Regulation 57 Pages: 293-301
  • Peer Reviewed
• [Journal Article] Purification and cDNA cloning of a wound inducible glucosyltransferase active toward 12-hydroxy jasmonic acid. (2009)
  • Author(s): Seto, Y.
  • Journal Title: Phytochemistry 70 Pages: 370-379
  • Peer Reviewed
• [Presentation] マクロアレイを用いたマイクロチューブハイブリダイゼーション法によるイチゴウイルスの同時検出 (2012)
  • Author(s): 古田和義
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2012-03-28
• [Presentation] タバコのウイルスに対する基盤免疫におけるカルモジュリン様タンパクrgs-CaMの役割 (2012)
  • Author(s): 中原健二
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2012-03-28
• [Presentation] キュウリモザイクウイルス感染に対するLRRドメインを介した抵抗性タンパク質RCY1の自己分解と防御応答システムの活性化 (2012)
  • Author(s): 高橋英樹
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2012-03-28
• [Presentation] Ascorbic acid accumulation is specifically induced in incompatible interaction between Brassica rapa and Turnip mosaic virus (2012)
  • Author(s): Fujiwara, A.
  • Organizer: 第2回日韓合同シンポジウム
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2012-03-27
• [Presentation] Rapid and convenient diagnosis of strawberry anthracnose using a method combining macroarray and microtube hybridization (2012)
  • Author(s): 長嶋沙希
  • Organizer: 第2回日韓合同シンポジウム
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2012-03-27
• [Presentation] Simultaneous detection of strawberry viruses using cDNA macroarray (2012)
  • Author(s): 古田和義
  • Organizer: 第2回日韓合同シンポジウム
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2012-03-27
• [Presentation] Virus-induced necrosis is a consequence of direct protein-protein in theraction between a viral RNA silencing suppressor and a host catalase (2012)
  • Author(s): Masuta, C.
  • Organizer: 第2回日韓合同シンポジウム
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2012-03-27
• [Presentation] Simultaneous detection of strawberry viruses using cDNA macroarray (2012)
  • Author(s): 古田和義
  • Organizer: 第2回日韓合同シンポジウム
  • Place of Presentation: 幅岡国際会議場(福岡市)
  • Year and Date: 2012-03-27
• [Presentation] Virus-induced necrosis is a consequence of direct protein-protein interaction between a viral RNA silencine suppressor and a host catalase (2012)
  • Author(s): Chikara Masuta
  • Organizer: 第2回日韓合同シンポジウム
  • Place of Presentation: 福岡国際会議場(福岡市)(招待講演)
  • Year and Date: 2012-03-27
• [Presentation] RNA-mediated epigenetic modifications provide a tool to produce a plant that does not carry a transgene but has altered traits (2012)
  • Author(s): Kanazawa, A.
  • Organizer: Japan-Australia Symposium on Plant Science for Agriculture
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-01-19
• [Presentation] Analysis of DNA-binding ability and phosphorylaiton of the 2b protein of Cucumber mosaic virus (2012)
  • Author(s): Sueda, K.
  • Organizer: Gene silencing by small RNAs In Keystone Symposia on Molecular and Cellular Biology
  • Place of Presentation: Fairmont Hotel Vancouver(バンクーバー,カナダ)
• [Presentation] キュウリモザイクウイルス2bタンパク質のリン酸化修飾と機能に対する影響 (2012)
  • Author(s): 末田香恵
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場(福岡市)
• [Presentation] Analysis of DNA-binding ability and phosphorylation of the 2b protein of Cucumber mosaic virus (2012)
  • Author(s): Sueda, K.
  • Organizer: Gene silencing by samll RN As.In Keystone Symposia on Molecular and Cellular Biology, Paster 322, Vancouver, Canada
  • Place of Presentation: Fairmont Hotel Vancouver(カナダ)
• [Presentation] RNA-mediatedepigenetic modifications of an endogenous gene as a tool to produce a plantthat does not carry a transgene but has altered traits (2011)
  • Author(s): Kanazawa, A.
  • Organizer: SOL&ICuGI 2011 8thSolanaceae and 2nd Cucurbi taceae Genome Joint Conference
  • Place of Presentation: 神戸コンベンションセンター(神戸市)
  • Year and Date: 2011-12-01
• [Presentation] タバコでのウイルス感染がレトロトランスポゾンTto1の発現に及ぼす影響 (2011)
  • Author(s): 竹田野土香
  • Organizer: 平成23年度日本植物病理学会北海道部会
  • Place of Presentation: 北海道農研センター(札幌市)
  • Year and Date: 2011-10-13
• [Presentation] タバコカルモジュリン様タンパク質rgs-CaMとウイルス外被タンパク質の相互作用 (2011)
  • Author(s): 忠村一毅
  • Organizer: 平成23年度日本植物病理学会北海道部会
  • Place of Presentation: 北海道農研センター(札幌市)
  • Year and Date: 2011-10-13
• [Presentation] イチゴウイルスのマクロアレイによる同時検出 (2011)
  • Author(s): 古田和義
  • Organizer: 平成23年度日本植物病理学会北海道部会
  • Place of Presentation: 北海道農研センター(札幌市)
  • Year and Date: 2011-10-13
• [Presentation] 外来遺伝子を持たずに特定の形質が変化した植物体の作出に必要なエピジェネティックな変化の促進 (2011)
  • Author(s): 金澤章
  • Organizer: 日本育種学会第120回講演会
  • Place of Presentation: 福井県立大学(福井県)
  • Year and Date: 2011-09-24
• [Presentation] エピジェネティックな変化を誘導することで遺伝子組換えによらずに形質を変化させた植物体の作出 (2011)
  • Author(s): 金澤章
  • Organizer: 日本育種学会第119回講演会
  • Place of Presentation: 横浜市立大学(横浜市)
  • Year and Date: 2011-04-14
• [Presentation] ニンニク鱗茎からの効率的なウイルス検出の確立 (2011)
  • Author(s): 吉田直人
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 東京農工大学(府中市)
  • Year and Date: 2011-03-27
• [Presentation] 感染葉におけるキュウリモザイクウイルス2bタンパク質のリン酸修飾解析 (2011)
  • Author(s): 末田香恵
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 東京農工大学(府中市)
  • Year and Date: 2011-03-27
• [Presentation] Brassica rapaにおけるTuMVに対するえそ誘導遺伝子座Rnt 1のマッピング (2011)
  • Author(s): 藤原綾香
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 東京農工大学(府中市)
  • Year and Date: 2011-03-27
• [Presentation] キュウリモザイクウイルスYサテライトRNAが誘導する黄化症状の分子メカニズムの解析 (2011)
  • Author(s): 志村華子
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 東京農工大学(府中市)
  • Year and Date: 2011-03-27
• [Presentation] he inheritable transcriptional gene silencing targeted to endogenous genes in petunia by using the Cucumber mosaic virus vector. (2011)
  • Author(s): Inaba, J.
  • Organizer: Keystone Symposia on Molecular and Cellular Biology
  • Place of Presentation: Monterey, California, USA
  • Year and Date: 2011-03-22
• [Presentation] Spatial effects of the 2b protein of cucumber mosaic virus on viral spread in infection dynamics (2011)
  • Author(s): M.Takeshita
  • Organizer: International Union of Microbiological Societies (IUMS) 2011 Congress, Sapporo
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
• [Presentation] Efficient detection and phylogenetic analysis of garlic viruses in Japan (2011)
  • Author(s): Yoshida, N.
  • Organizer: International Union of Microbiological Societies (IUMS) 2011 Congress, Sapporo
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
• [Presentation] Novel expression system to confine the cucumber mosaic virus vector in the infected transgenic plants (2011)
  • Author(s): Noriho Fukuzawa
  • Organizer: International Union of Microbiological Societies (IUMS) 2011 Congress, Sapporo
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
• [Presentation] Development of self-assembling protein production system by the combination of two plant viral vectors (2011)
  • Author(s): Kazunori Goto
  • Organizer: International Union of Microbiological Societies (IUMS) 2011 Congress, Sapporo
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
• [Presentation] Possible role of endogenous ascorbic acids in Brassica rapa in defence against turnip mosaic virus (2011)
  • Author(s): Ayaka Fujiwara
  • Organizer: International Union of Microbiological Societies (IUMS) 2011 Congress, Sapporo
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
• [Presentation] Risk-managed production of bioactive recombinant proteins using a nove 1 plant virus confinement system (2011)
  • Author(s): N.Itchoda
  • Organizer: Plant based vaccines & antibodies 2011
  • Place of Presentation: Tiara Park Atlantico Hotel(ポルトガル)
• [Presentation] The combination of the Cucumber mosaic virus vector and HC-Pro transgenic plants is an efficient system for production of a foreign protein in N.benthamianaplants (2011)
  • Author(s): N.Fukuzawa
  • Organizer: Plant based vaccines & antibodies 2011
  • Place of Presentation: Tiara Park Atlantico Hotel(ポルトガル)
• [Presentation] Screening of antiviral agents to inhibit RNA silencing suppressors of plant and animal viruses. (2010)
  • Author(s): Fujiwara, A.
  • Organizer: Antivirals Congress
  • Place of Presentation: Amsterdam, Nederland
  • Year and Date: 2010-11-07
• [Presentation] 植物ウイルスベクターを介して誘導される外来性遺伝子のプロモーター領域のメチル化状態と転写型ジーンサイレンシングの安定性 (2009)
  • Author(s): 太田垣駿吾
  • Organizer: 日本育種学会第116回講演会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-10-26
• [Presentation] カブモザイクウイルスに感染したハクサイにおける病徴決定因子 (2009)
  • Author(s): 藤原綾香
  • Organizer: 平成21年度植物感染生理談話会
  • Place of Presentation: 大沼国際セミナーハウス(函館)
  • Year and Date: 2009-08-07
• [Presentation] キュウリモザイクウイルスベクターに挿入する配列の長さと外来性遺伝子の効率的転写不活性化誘導の関連性 (2009)
  • Author(s): 太田垣駿吾
  • Organizer: 平成21年度植物感染生理談話会
  • Place of Presentation: 大沼国際セミナーハウス(函館)
  • Year and Date: 2009-08-07
• [Presentation] キュウリモザイクウイルスによって誘導されたペチュニア内在性遺伝子のプロモーター領域のメチル化とヒストン修飾の解析 (2009)
  • Author(s): 稲場純一
  • Organizer: 平成21年度植物感染生理談話会
  • Place of Presentation: 大沼国際セミナーハウス(函館)
  • Year and Date: 2009-08-07
• [Presentation] Viral non-proliferation system to produce a valuable protein in transgenic plants. (2009)
  • Author(s): 福澤徳穂
  • Organizer: 3th Internalional Conference of Plant-Based Vaccine & Antibody
  • Place of Presentation: ヴェローナ(イタリア)
• [Presentation] CMVベクターによる高効率な物質生産が可能な植物体の作出とサイクリックなCMV蓄積への影響 (2009)
  • Author(s): 石原岳明
  • Organizer: 平成21年度日本植物病理学会大会北海道部会
  • Place of Presentation: 北海道大学
• [Presentation] サブグループ識別(SS)-RT-PCRによるリアソータントCMVの検出 (2009)
  • Author(s): 眞岡哲夫
  • Organizer: 平成21年度日本植物病理学会大会北海道部会
  • Place of Presentation: 北海道大学
• [Presentation] オオムギいもち病抵抗性の遺伝 (2009)
  • Author(s): 犬飼剛
  • Organizer: 平成21年度日本植物病理学会北海道部会談話会
  • Place of Presentation: 北海道大学
• [Presentation] Cucumber mosaic virus vector has the ability to induce inheritable transcriptional gene silencing of endogenes in petunia. (2009)
  • Author(s): 稲場純一
  • Organizer: The 1^<st> Japan-Korea joint symposium
  • Place of Presentation: Jeju, Korea
• [Presentation] The P3 of Turnip mosaic virus in an avirulence determinat for TuNI in Arabidopsis thaliana to induce HR-like cell death. (2009)
  • Author(s): キム・ボミン
  • Organizer: The 1^<st> Japan-Korea joint symposium
  • Place of Presentation: Jeju, Korea