| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2009: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
|
|---|
| Research Abstract |
Based on the study on the mechanism of accumulation and degradation of foreign protein expressed in plant cells, we got the following results. Using color conversible fluorescenct protein fluorescence conversion, we have developed a method to distinguish between pre-existed and nascent proteins. Using this system we found that induction of the degradation of intracellular protein aggregates by autophagy by phosphate starvation was specifically inhibited by phosphite and this inhibition was not observed in nitrogen and sugar starvation conditions. We also found that phosphite dependent inhibition of the degradation under phosphate-limitation condition depends on proteins.
|
