The development of a mini-array specific to gastric cancers to estimate biological characteristics

• Project/Area Number: 21390106
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Yamaguchi University
• Principal Investigator: SASAKI Kohsuke 山口大学, 大学院・医学系研究科, 教授 (80116722)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2009: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
• Keywords: がん / ゲノム / DNAコピー数 / アレイCGH / CGH / WGAS / ミニアレイ / 胃癌 / 大腸癌 / 癌病態 / 悪性度

Research Abstract

We developed the" mini-array" for gastric cancer to evaluate biological characteristics including lymph node metastasis and so on. In order to select BAC clones linked with biological characteristics, we applied a technology of array-based CGH to gastric cancers. To evaluate the" mini-array" for gastric cancer, we applied" mini-array" for another case of gastric cancers and we succeeded in predicting correctly about 70% of cases with no lymph node metastasis. This result suggests the" mini-array" a useful device for personalized treatment for cancers.

Research Products

• [Journal Article] Loss of 6q or 8p23 is associated with the total number of DNA copy number aberrations in adenoid cystic carcinoma (2011)
  • Author(s): Oga A, Uchida K, Nakao M, Kawauchi S, Furuya T, Chochi Y, Ikemoto K, Okada T, Ueyama Y, Sasaki K, Yousefpour F
  • Journal Title: Oncol Rep Volume: 26(6) Pages: 1393-1398
  • DOI: 10.3892/or.2011.1446
  • Peer Reviewed
• [Journal Article] Centrosome amplification as a putative prognostic biomarker for the classification of urothelial carcinomas (2011)
  • Author(s): Yamamoto Y, Misumi T, Eguchi S, Nakao M, Hara T, Sakano S, Furuya T, Oga A, Kawauchi S, Sasaki K, Matsuyama H
  • Journal Title: Hum Pathol Volume: 42(12) Pages: 1923-1930
  • Peer Reviewed
• [Journal Article] Loss of 3p26. 3 is an independent prognostic factor in patients with oral squamous cell carcinoma (2011)
  • Author(s): Uchida K, Oga A, Nakao M, Mano T, Mihara M, Kawauchi S, Furuya T, Ueyama Y, Sasaki K
  • Journal Title: Oncol Rep Volume: 26(2) Pages: 463-469
  • Peer Reviewed
• [Journal Article] DNA copy number aberrations associated with the clinicopathological features of colorectal cancers : Identification of genomic biomarkers by array-based comparative genomic hybridization (2011)
  • Author(s): Nakao M, Kawauchi S, Uchiyama T, Adachi J, Ito H, Chochi Y, Furuya T, Oga A, Sasaki K
  • Journal Title: Oncol Rep Volume: 25(6) Pages: 1603-1611
  • DOI: 10.3892/or.2011.1246
  • Peer Reviewed
• [Journal Article] A simple method for enhancing hybridization efficiency in chromosome and array comparative genomic hybridization (2011)
  • Author(s): Kawauchi S, Furuya T, Nakao M, Ikemoto K, Oga A, Sasaki K
  • Journal Title: Biotech Histochem Volume: 86(3) Pages: 192-198
  • Peer Reviewed
• [Journal Article] Loss of 3p26.3 is an independent prognostic factor in patients with oral squamous cell carcinoma (2011)
  • Author(s): Uchida K.
  • Journal Title: Oncology Report Volume: 26 Pages: 463-469
  • DOI: 10.3892/or.2011.1327
  • Peer Reviewed
• [Journal Article] Centrosome ampliication as a putative prognostic biomarker for the classification of urothelial carcinomas (2011)
  • Author(s): Yamamoto Y
  • Journal Title: human pathology Volume: 12 Pages: 1923-1930
  • Peer Reviewed
• [Journal Article] DNA copy number aberrations associated with the clinicopathological features of colorectal cancers : Identification of genomic biomarkers by array-based comparative genomic hybridization. (2011)
  • Author(s): Nakao M
  • Journal Title: Oncology Report Volume: 25 Pages: 1603-1611
  • Peer Reviewed
• [Journal Article] Screening for DNA copy number aberrations in mucinous adenocarcinoma arising from the minor salivary gland : two case reports (2010)
  • Author(s): Uchida K, Oga A, Nagatsuka H, Ueyama Y, Sasaki K
  • Journal Title: Cancer Genet Cytogenet Volume: 203(2) Pages: 324-327
  • Peer Reviewed
• [Journal Article] DNA copy number aberrations associated with aneuploidy and chromosomal instability in breast cancers (2010)
  • Author(s): Kawauchi S, Furuya T, Ikemoto K, Yamamoto S, Oka M, Sasaki K
  • Journal Title: Oncol Rep Volume: 24(4) Pages: 875-883
  • Peer Reviewed
• [Journal Article] Genomic instability and DNA ploidy are linked to DNA copy number aberrations of 8p23 and 22q11. 23 in gastric cancers (2010)
  • Author(s): Kawauchi S, Furuya T, Uchiyama T, Adachi A, Okada T, Nakao M, Oga A, Uchida K, Sasaki K
  • Journal Title: Int J Mol Med Volume: 26(3) Pages: 333-339
  • Peer Reviewed
• [Journal Article] The loss of 8p23. 3 is a novel marker for predicting progression and recurrence of bladder tumors without muscle invasion (2010)
  • Author(s): Eguch S, Yamamoto Y, Sakano S, Chochi Y, Nakao M, Kawauchi S, Furuya T, Oga A, Matsuyama H, Sasaki K
  • Journal Title: Cancer Genet Cytogenet Volume: 200(1) Pages: 16-22
  • Peer Reviewed
• [Journal Article] Genetic alterations at 13q14 may correlate with differences in the biological behavior of prostate cancer between Japanese and Caucasian men (2010)
  • Author(s): Misumi T, Yamamoto Y, Murakami T, Ito H, Eguchi S, Yano S, Nagao K, Sakano S, Naito K, Sasaki K, Fichtner J, Matsuyama H
  • Journal Title: Urol Int Volume: 84(4) Pages: 461-466
  • Peer Reviewed
• [Journal Article] Breast cancer cell lines carry cell line-specific genomic alterations that are distinct from aberrations in breast cancer tissues : comparison of the CGH profiles between cancer cell lines and primary cancer tissues (2010)
  • Author(s): Tsuji K, Kawauchi S, Saito S, Furuya T, Ikemoto K, Nakao M, Yamamoto S, Oka M, Hirano T, Sasaki K
  • Journal Title: BMC cancer Volume: 14 Pages: 10-15
  • Peer Reviewed
• [Journal Article] Genomic instability and DNA ploidy are linked to DNA copy number aberrations of 8p23 and 22q11.23 in gastric cancers. (2010)
  • Author(s): Kawauchi S
  • Journal Title: Int J Mol Med Volume: 26 Pages: 333-339
  • Peer Reviewed
• [Journal Article] Connexin 26 expression correlates with less aggressive phenotype of intestinal type-gastric carcinomas. (2010)
  • Author(s): Liu XL
  • Journal Title: Int J Mol Med Volume: 25 Pages: 709-716
  • Peer Reviewed
• [Journal Article] 9p21 index as estimated by dual-color fluorescence in situ hybridization is useful to predict urothelial carcinoma recurrence in bladder washing cytology (2009)
  • Author(s): Kawauchi S, Sakai H, Ikemoto K, Nakao M, Takihara H, Shimabukuro T, Furuya T, Oga A, Matsuyama H, Sasaki K
  • Journal Title: Hum Pathol Volume: 40(12) Pages: 1783-1789
  • NAID: 120001613389
  • Peer Reviewed
• [Journal Article] Intercellular centrosome number is correlated with the copy number of chromosomes in bladder cancer (2009)
  • Author(s): Yamamoto Y, Eguchi S, Nagao K, Furuya T, Oga A, Kawauchi S, Sasaki K, Matsuyama H
  • Journal Title: Cancer Genet Cytogenet Volume: 191(1) Pages: 38-42
  • NAID: 120002647753
  • Peer Reviewed
• [Journal Article] A copy number gain of the 6p arm is linked with advanced hepatocellular carcinoma an array-based comparative genomic hybridization study (2009)
  • Author(s): Chochi Y, Kawauchi S, Furuya T, Hasimoto K, Oga A, Sasaki K
  • Journal Title: J Pathol Volume: 217(5) Pages: 677-684
  • NAID: 120002619695
  • Peer Reviewed
• [Journal Article] 9p21 index as estimated by dual-color fluorescence in situ hybridization is useful to predict urothelial carcinoma recurrence in bladder washing cytology. (2009)
  • Author(s): Kawauchi S
  • Journal Title: Hum Pathol. 40 Pages: 1783-1789
  • Peer Reviewed
• [Journal Article] A copy number gain of the 6p arm is linked with advanced hepatocellular carcinoma : an array-based comparative genomic hybridization study. (2009)
  • Author(s): Chochi Y
  • Journal Title: J Pathol 217 Pages: 677-684
  • Peer Reviewed
• [Journal Article] Intercellular centrosome number is correlated with the copy number of chromosomes in bladder cancer. (2009)
  • Author(s): Yamamoto Y
  • Journal Title: Cancer Genet Cytogenet 191 Pages: 38-42
  • Peer Reviewed
• [Presentation] がん細胞とゲノム発現 (2011)
  • Author(s): 佐々木功典
  • Organizer: 第52回日本臨床細胞学会総会
  • Place of Presentation: 福岡福岡国際会議場
  • Year and Date: 2011-05-21
• [Presentation] ゲノム解析のがん診療への応用 (2010)
  • Author(s): 佐々木功典
  • Organizer: 第65回日本消化器外科学会
  • Place of Presentation: 下関海峡メッセ下関
  • Year and Date: 2010-07-14
• [Presentation] ゲノム解析のがん診療への応用 (2010)
  • Author(s): 佐々木功典
  • Organizer: 第65回日本消化器外科学会
  • Place of Presentation: 海峡メッセ下関
  • Year and Date: 2010-07-14
• [Presentation] 癌とゲノム (2010)
  • Author(s): 佐々木功典
  • Organizer: 第20回日本サイトメトリー学会学術集会
  • Place of Presentation: 東京東京慈恵会医科大学
  • Year and Date: 2010-06-26
• [Presentation] 癌とゲノム (2010)
  • Author(s): 佐々木功典
  • Organizer: 第20回日本サイトメトリー学会学術集会
  • Place of Presentation: 東京慈恵医科大学
  • Year and Date: 2010-06-26
• [Presentation] 大腸癌74例のアレイCGH解析およびMDR(multifactor dimensionality reduction)法解析 (2009)
  • Author(s): 中尾素直
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-05-02