Molecular mechanism on anti-angiogenic effect of siRNA in choroidal neovascularization

• Project/Area Number: 21390469
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Nagoya City University
• Principal Investigator: OGURA Yuichiro 名古屋市立大学, 大学院・医学研究科, 教授 (70191963)
• Co-Investigator(Kenkyū-buntansha): YASUKAWA Tsutomu 名古屋市立大学, 大学院・医学研究科, 准教授 (00324632) ; NOZAKI Miho 名古屋市立大学, 大学院・医学研究科, 講師 (00295601) ; MATSUBARA Akihisa 名古屋市立大学, 大学院・医学研究科, 助教 (80336685) ; 吉田 宗徳 名古屋市立大学, 医学(系)研究科(研究院), 准教授 (60273447)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2009: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: 加齢黄斑変性 / 脈絡膜新生血管 / アテロコラーゲン / VEGF / RNA 干渉(siRNA) / RNA干渉（siRNA） / TLR3 / クロロキン / IFN-γ / RNA干渉(siRNA) / 脈絡膜新生血管(CNV) / 加齢黄斑変性症 / VEGF-A

Research Abstract

Previous studies have shown that siRNAs could suppress angiogenesis via stimulation of toll-like receptor (TLR) -3. The purpose of this study was to determine the efficacy of atelocollagen to deliver siRNA without TLR3 stimulation in the laser-induced choroidal neovascularization (CNV) model. The mean CNV volumes were significantly smaller in the naked siRNA-Luc, naked siRNA-Vegfa, or siRNA-Vegfa /atelocollagen complex compared with PBS, atelocollagen, or siRNA-Luc/atelocollagen complex-injected mice (p < 0.05). These findings demonstrate that atelocollagen may deliver siRNA without non-specific TLR3 stimulation in the murine laser-CNV model.

Research Products

• [Journal Article] Non-targeted siRNA suppresses laser-induced choroidal neovascularization. (2010)
  • Author(s): Ashikari M, Tokoro M, Itaya M, Nozaki M, Ogura Y
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 51巻 Pages: 3820-3824
  • Peer Reviewed
• [Journal Article] Non-targeted siRNA suppresses laser-induced choroidal neovascularization (2010)
  • Author(s): Ashikari M, Tokoro M, Itaya M, Nozaki M, Ogura Y
  • Journal Title: Invest Ophthalmol Vis Sci (Epub)
  • Peer Reviewed
• [Journal Article] CCR3 is a target for age-related macular degeneration diagnosis and therapy (2009)
  • Author(s): Takeda A, Nozaki M, Ogura Y, et al
  • Journal Title: Nature 460 Pages: 225-230
  • Peer Reviewed
• [Presentation] The Efficient Delivery of siRNA by Atelocollagen on Laser-induced Choroidal Neovascularization Model in Mice (2011)
  • Author(s): Yuya Ito, Miho Nozaki, Kazuhiko Sugitani, Masayuki Ashikari, Yoshio Hirano, Yuichiro Ogura
  • Organizer: ARVO
  • Place of Presentation: フォートローダーデール(アメリカ)
• [Presentation] Anti-angiogenic effect of IFN- γ and IL-12 on laser-induced choroidal neovascularization model in mice. (2011)
  • Author(s): Masayuki Ashikari, Miho Nozaki, Takeshi Mizutani, Yuichiro Ogura
  • Organizer: ARVO
  • Place of Presentation: フォートローダーデール(アメリカ)
• [Presentation] CCR3 antagonist suppresses laser-induced choroidal neovascularization in mice. (2010)
  • Author(s): Ashikari M, Tokoro M, Nozaki M, Ogura Y
  • Organizer: Euretina
  • Place of Presentation: パリコンベンションセンター(フランス)
  • Year and Date: 2010-09-03