Detection of Circulating Endothelial Cells in Severe Sepsis

Research Project

Project/Area Number	21390482
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Research Field	Emergency medicine
Research Institution	Kyoto University
Principal Investigator	KOIKE Kaoru 京都大学, 医学研究科, 教授 (10267164)
Co-Investigator(Kenkyū-buntansha)	MATSUDA Naoyuki 名古屋大学, 医学研究科, 教授 (50332466)
Project Period (FY)	2009 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000) Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000) Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000) Fiscal Year 2009: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Keywords	敗血症 / 遊離型血管内皮細胞 / CEC / AP-1 / TAK-1 / NF-κB / 播種性血管愛凝固症候群 / DIC / 血管内皮細胞 / アポトーシス / ショック / 遺伝子核酸治療 / マウス / Death受容体
Research Abstract	Sepsis is a severe disorder as systemic inflammation with infection. In this illness, vascular endothelial damage proceeds to induce disseminated intravascular coagulation(DIC). This study revealed that circulating endothelial cell(CEC) was increased and plasma platelet count was decreased in the time dependent manner after sepsis induction by cecal ligation and puncture in male BALB-C mice. Nuclear factor-kappaB decoy oligonucleotides more strongly decreased CEC count than inactivation of FADD, TAK-1, and activator protein-1. The CEC count could be a reliable marker to evaluate DIC and dysfunction of vascular endothelium.