| Project/Area Number |
21390528
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
HONDA Masaki 日本大学, 歯学部, 准教授 (70361623)
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| Co-Investigator(Kenkyū-buntansha) |
ISOKAWA Keitaro 日本大学, 歯学部, 教授 (50168283)
YUGUCHI Maki 日本大学, 歯学部, 助手 (00256885)
MIKAMI Yoshikazu 日本大学, 歯学部, 助教 (80434075)
山崎 洋介 日本大学, 歯学部, 助教 (20366610)
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| Co-Investigator(Renkei-kenkyūsha) |
WATANABE Nobukazu 東京大学, 医科学研究所, 准教授 (10334278)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Keywords | 再生歯学 / 歯胚 / 歯根再生 / 歯周組織 / セメント質 / 歯小嚢細胞 / 象牙質 / 再生 / 上皮細胞 / 組織工学 / 歯髄細胞 / 担体 / ポリグリコール酸 / 幹細胞 / 象牙質再生 / 分化誘導 |
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| Research Abstract |
We performed a series of experiment for a development of method to generate a tooth-root with periodontium in the limited three years. In the first years, we developed the method to isolate and culture the dental follicle-derived cells, human periodontal ligament-derived, and human pulp-derived mesenchymal cells. In the second year, the suitable scaffolds to generate dentin tissues were examined using dental pulp-derived mesenchymal cells. In the last year, we create the bone trunk and implanted the mimic tooth germ including enamel organ-derived, dental pulp-derived, and dental follicle-derived cells in the bone trunk. After implantation, periodontal ligament-like tissue on the surface of cementum-like tissues was observed in the bone trunk.
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