| Project/Area Number |
21390546
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
|
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| Research Institution | Meikai University (2010-2011)
Tokyo Medical and Dental University (2009) |
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Principal Investigator |
SUDA Naoto 明海大学, 歯学部, 教授 (90302885)
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| Co-Investigator(Kenkyū-buntansha) |
ISEKI Sachiko 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (80251544)
OHTA Masato 東京医科歯科大学, 大学院・医歯学総合研究科, 講師 (70313228)
MATSUI Shigeyuki 明海大学, 歯学部, 准教授 (40190392)
YOSHIKAWA Masayoshi 明海大学, 歯学部, 講師 (80200963)
MANO Mikiko 明海大学, 歯学部, 講師 (00333005)
SAKURAI Yousuke 明海大学, 歯学部, 助教 (20529630)
SASAKI Au 明海大学, 歯学部, 助教 (60580230)
KOUHARA Manabu 明海大学, 歯学部, 助教 (40602529)
|
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2009: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | iPS細胞 / 歯根 / 組織再生 / 3次元構築 / 形態異常 / 上顎骨 / 下顎骨 / DSPP / TGF-β / 歯根吸収 / 再生医療 |
|---|
| Research Abstract |
The human specific TaqMan probes for DSPP and GAPDH which were not recognized by mouse sequences were designed. To test the appropriate feeder cells for the differentiation of iPS cells to DSPP expressing cells, mouse embryonic fibroblasts were found to be most potent. More than 10 growth factors and cytokines were tested for the differentiation, and TGF-ss was the most effective molecule. An analyzing system for the three-dimensional tooth root structure using DICOM data was also established.
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