The possible role of autophagy in craniofacial development

• Project/Area Number: 21390547
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Okayama University
• Principal Investigator: YAMASHIRO Takashi 岡山大学, 大学院・医歯薬学総合研究科, 教授 (70294428)
• Co-Investigator(Kenkyū-buntansha): YABUUCHI Toshinori 岡山大学, 岡山大学病院, 医員 (00452582) ; KAWABE Noriaki (KUROSAKA Hiroshi) 岡山大学, 岡山大学病院, 講師 (00397879) ; NAKAGAWA Ichiro 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (70294113) ; 黒坂 寛 岡山大学, 大学院・医歯薬学総合研究科, 助教 (20509369)
• Co-Investigator(Renkei-kenkyūsha): MIZUSHIMA Noboru 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (10353434)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2009: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: オートファジー / 口蓋の形成 / 口蓋突起 / 骨組織 / ERストレス / ATG5 / LC3 / 小胞体ストレス / 口唇口蓋裂 / 骨形成 / 口蓋 / 顎顔面 / 骨格 / 骨芽細胞

Research Abstract

Autophagy is a bulk protein degradation system and important for various physiological processes such as cell survival under starvation, development, immune responses, tumor suppression, and antiaging. The present study demonstrated that autophagy is involved in the palatal fusion in palatogenesis. Our study also indicated autophagy is also involved in osteogeneis and suggested that autopahgy might regulate the ER stress responses presumably to maintain cellular homeostasis.

Research Products

• [Journal Article] Roles of heparan sulfate sulfation in dentinogenesis (2012)
  • Author(s): Hayano S, Kurosaka H, Yanagita T, Kalus I, Milz F, Ishihara Y, Islam MN, Kawanabe N, Saito M, Kamioka H, Adachi T, Dierks T, Yamashiro T
  • Journal Title: J Biol Chem Volume: 287(15) Pages: 12217-29
  • Peer Reviewed
• [Journal Article] Stage-specific embryonic antigen-4 identifies human dental pulp stem cells (2012)
  • Author(s): Kawanabe N, Murata S, Fukushima H, Ishihara Y, Yanagita T, Yanagita E, Ono M, Kurosaka H, Kamioka H, Itoh T, Kuboki T, Yamashiro T
  • Journal Title: Exp Cell Res Volume: 318(5) Pages: 453-63
  • Peer Reviewed
• [Journal Article] Core binding factor beta functions in the maintenance of stem cells and orchestrates continuous proliferation and differentiation in mouse incisors (2011)
  • Author(s): Kurosaka H, Islam MN, Kuremoto K, Hayano S, Nakamura M, Kawanabe N, Yanagita T, Rice DP, Harada H, Taniuchi I, Yamashiro T
  • Journal Title: Stem Cells Volume: 29(11) Pages: 1792-803
  • Peer Reviewed
• [Journal Article] Sox9 expression during fracture repair (2011)
  • Author(s): Shintaku Y, Murakami T, Yanagita T, Kawanabe N, Fukunaga T, Matsuzaki K, Uematsu S, Yoshida Y, Kamioka H, Takano-Yamamoto T, Takada K, Yamashiro T
  • Journal Title: Cells Tissues Organs Volume: 194(1) Pages: 38-48
  • Peer Reviewed
• [Journal Article] Sox9 Expression during Fracture Repair.
  • Author(s): Shintaku Y, Murakami T, Yanagita T, Kawanabe N, Fukunaga T, Matsuzaki K, Uematsu S, Yoshida Y, Kamioka H, Takano-Yamamoto T, Takada K, Yamashiro T.
  • Journal Title: Cells Tissues Organs Volume: (in press)
  • Peer Reviewed
• [Journal Article] The Three-Dimensional Morphometry and Cell-Cell Communication of the Osteocyte Network in Chick and Mouse Embryonic Calvaria.
  • Author(s): Sugawara Y, Ando R, Kamioka H, Ishihara Y, Honjo T, Kawanabe N, Kurosaka H, Takano-Yamamoto T, Yamashiro T.
  • Journal Title: Calcif Tissue Int Volume: (in press)
  • Peer Reviewed
• [Presentation] 骨芽細胞の石灰化が進むにつれ、オートファジーとERストレスマーカーは上昇する (2012)
  • Author(s): 中村政裕, 柳田剛志, 山城隆
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岐阜
• [Presentation] 骨芽細胞の石灰化が進むにつれ、オートファジーとERストレスマーカーは上昇する (2011)
  • Author(s): 中村政裕, 柳田剛志, 山城隆
  • Organizer: 第53回日本歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 岐阜市
  • Year and Date: 2011-10-01
• [Remarks] (1) 2011年先端歯学にて成果の報告。中村政裕,山城隆、骨形成におけるオートファジーの役割。平成23年度先端歯学スクール2011、2011年9月1-2日、三浦