| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Research Abstract |
SDS-digested freeze-fracture replica labeling(SDS-FRL) was innovated as a suitable technique to reveal the fine structure of plasma membrane and quantitative localization of biomolecules over the membrane structure. However, it was difficult to apply this technique to molecules immobilized on protoplasmic faces(P-face) quantitatively. Thus, in this research project, I tried to optimize labeling protocols of SDS-FRL. The optimization was carried out at steps of SDS treatment, pretreatment and blocking of replicated membranes as well as components on each labeling solutions. As a result, although details of labeling protocol differ among experiments, new protocol provided high-sensitive labeling not only for molecules on exoplasmic faces but also for those on P-faces. Localization data obtained by these investigations were published as original papers.
|
