Biochemical analyses of Ufm1-modification of proteins in the synapse

• Project/Area Number: 21500347
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Shinshu University
• Principal Investigator: SUZUKI Tatsuo 信州大学, 医学系研究科, 教授 (80162965)
• Co-Investigator(Kenkyū-buntansha): TANAHASHI Hiroshi 信州大学, 医学系研究科, 准教授 (90236654)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: シナプス / PSD / シナプス後肥厚部 / タンパク質修飾

Research Abstract

Ufm1(Ubiquitin-fold modifier 1) is a most recently identified ubiquitination-like protein modification tag. Using anti-Ufc1(Ufm-conjugating E2-like enzyme) and anti-Ufm1 antibodies that were produced by us, localization of Ufc1 and Ufm1 in the brain and cultures neurons was identified. The substrate proteins that receive Ufm1-modification were surveyed in the brain.

Research Products

• [Journal Article] SynArfGEF is a guanine nucleotide exchange factor for Arf6 and localizes preferentially at post-synaptic specializations of inhibitory synapses (2011)
  • Author(s): Fukaya, M.; Kamata, A.; Hara, Y., et al
  • Journal Title: Journal of Neurochemistry Volume: 116(6) Issue: 6 Pages: 1122-1137
  • DOI: 10.1111/j.1471-4159.2010.07167.x
  • Peer Reviewed
• [Journal Article] Association of Membrane Rafts and Postsynaptic Density : Proteomics, Biochemical, and Ultrastructural Analyses: The ROAD Study (2011)
  • Author(s): Tatsuo Suzuki, Jingping Zhang, Shoko Miyazawa, Qian Liu, Michael R. Farzan, Wei-Dong Yao
  • Journal Title: J. Neurochem Volume: 119 Issue: 1 Pages: 64-77
  • DOI: 10.1111/j.1471-4159.2011.07404.x
  • Peer Reviewed
• [Journal Article] タンパク質合成と可塑性 (2011)
  • Author(s): 鈴木龍雄
  • Journal Title: Clinical Neuroscience Volume: 29 Pages: 763-765
• [Journal Article] タンパク質合成と可塑性 (2011)
  • Author(s): 鈴木龍雄
  • Journal Title: Clinical Neuroscience 29 Volume: 29 Pages: 763-765
• [Journal Article] Regulation of CaMKII by Phospho-Thr253 or Phospho-Thr286 Sensitive Targeting Alters Cellular Function (2010)
  • Author(s): Kathryn A. Skelding, Tatsuo Suzuki, Sarah Gordon, Jing Xue, Nicole M. Verrills, Phillip W. Dickson, John A. P. Rostas
  • Journal Title: Cellular Signalling Volume: 22 Issue: 5 Pages: 759-769
  • DOI: 10.1016/j.cellsig.2009.12.011
  • Peer Reviewed
• [Journal Article] シナプスをめぐるシグナリング (2010)
  • Author(s): 棚橋浩, 鈴木龍雄
  • Journal Title: 生体の科学 Volume: 61 Pages: 392-393
  • URL: http://www.igaku-shoin.co.jp/journalPortal.do?journalPortalId=425
• [Journal Article] MafB proteln stability is regulated by the JNK and ubiquitin-proteasome pathways. (2010)
  • Author(s): Tanahashi, H., Kito, K., Ito, T., Yoshioka, K.
  • Journal Title: Arch.Biochem.Biophys. Volume: 494 Pages: 94-100
  • Peer Reviewed
• [Journal Article] SynArfGEF is a guanine nucleotide exchange factor for Arf6 and localise preferentially at postsynaptic specializations of inhibitory synapses. (2010)
  • Author(s): Fukaya M, Kamata A, Hara Y, Tamaki H, Katsumata O,Ito N, Takeda S, Hata Y, Suzuki T, Watanabe M, Harvey RJ, Sakagami H
  • Journal Title: J.Neurochem. Volume: 116 Pages: 1122-1137
  • Peer Reviewed
• [Journal Article] シナプス形成におけるLRP4シグナリングの関与 (2010)
  • Author(s): 棚橋浩, 鈴木龍雄
  • Journal Title: 生体の科学 Volume: 61 Pages: 392-393
• [Journal Article] synGAPアイソフォームの機能分化 (2010)
  • Author(s): 鈴木龍雄
  • Journal Title: 生体の科学 Volume: 61 Pages: 428-429
• [Journal Article] TANC1:スパインサイズ制御系との関連 (2010)
  • Author(s): 鈴木龍雄
  • Journal Title: 生体の科学 Volume: 61 Pages: 500-501
• [Journal Article] 脳では未開拓のmultivalent scaffolding protein, p55 (2010)
  • Author(s): 鈴木龍雄
  • Journal Title: 生体の科学 Volume: 61 Pages: 510-511
• [Journal Article] BAALC1-6-8proteinはカルモジュリンキナーゼαsubunitターゲッティングに関与する? (2010)
  • Author(s): 鈴木龍雄
  • Journal Title: 生体の科学 Volume: 61 Pages: 514-515
• [Journal Article] Regulation of CaMKII by Phospho-Thr253 or Phospho-Thr286 Sensitive Targeting Alters Cellular Function. (2010)
  • Author(s): Skelding K.A., Suzuki T., Gordon S., Xue J., Verrills N.M., Dickson P.W., Rostas.A.P.
  • Journal Title: Cellular Signalling 22 Pages: 759-769
  • Peer Reviewed
• [Journal Article] 新規シナプス後部タンパク質の同定と機能の解明:特にLRP4研究の進展について (2009)
  • Author(s): 鈴木龍雄
  • Journal Title: 信州医学会誌 Volume: 57 Pages: 63-70
  • NAID: 130004551749
  • URL: http://www.igaku-shoin.co.jp/journalPortal.do?journalPortalId=425
• [Journal Article] 新規シナプス後部タンパク質の同定と機能の解明:特にLRP4研究の進展について (2009)
  • Author(s): 鈴木龍雄
  • Journal Title: 信州医学会誌 57 Pages: 63-70
  • NAID: 130004551749
• [Presentation] シナプス後肥厚部-メンブランラフト複合体の解析 (2011)
  • Author(s): Tatsuo Suzuki, Qian Liu
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Association of postsynaptic density and membrane raft domainシナプス後肥厚部-メンブランラフト複合体の解析 (2011)
  • Author(s): Tatsuo Suzuki, Qian Liu
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Analysis of association of PSD with postsynaptic membrane rafts (2011)
  • Author(s): Suzuki T, Liu Q
  • Organizer: SFN
  • Place of Presentation: Washington
  • Year and Date: 2011-11-13
• [Presentation] Colocalization of Hippocampal Cholinergic Neurostimulating Peptide (HCNP) precursor protein with collapsin response mediator protein (CRMP)-2 at pre synapse in the hippocampus (2011)
  • Author(s): Kato D, Matsukawa N, Kanamori T, Mizuno M, Suzuki T, Ojika K
  • Organizer: Society for Neuroscience
  • Place of Presentation: Washington
  • Year and Date: 2011-11-13
• [Presentation] Colocalization of Hippocampal Cholinergic Neurostimulating Peptide(HCNP) precursor protein with collapsin response mediator protein(CRMP)-2 at pre synapse in the hippocampus (2011)
  • Author(s): Kato D, Matsukawa N, Kanamori T, Mizuno M, Suzuki T, Ojika K
  • Organizer: SFN
  • Place of Presentation: Washington
  • Year and Date: 2011-11-12
• [Presentation] Analysis of association of PSD with postsynaptic membrane rafts (2011)
  • Author(s): Suzuki T, Liu Q
  • Organizer: Society for Neuroscience
  • Place of Presentation: Washington
  • Year and Date: 2011-11-12
• [Presentation] Association of membrane rafts and postsynaptic density : Proteomics, biochemical, and ultrastructural analyses (2010)
  • Author(s): Suzuki, T., Zhang, J-P., Farzan, M. R., Miyazawa, S., Liu, Q., Yao W-D
  • Organizer: SFN
  • Place of Presentation: San Diego
  • Year and Date: 2010-11-15
• [Presentation] Association of membrane rafts and postsynaptic density: Proteomics, biochemical, and ultrastructural analyses. (2010)
  • Author(s): Suzuki T, Zhang J, Farzan MR, Miyazawa S, Liu Q, Yao W
  • Organizer: Society for Neuroscience
  • Place of Presentation: San Diego
  • Year and Date: 2010-11-15
• [Presentation] Analysis of postsynaptic membrane rafts and comparison with postsynaptic density prepared from synaptic plasma membrane (2010)
  • Author(s): Suzuki, T., Zhang, J-P., Farzan, M. R., Miyazawa, S., Liu, Q., Yao W-D
  • Organizer: APSN2010
  • Place of Presentation: Phuket, Thai
  • Year and Date: 2010-10-18
• [Presentation] Analysis of postsynaptic membrane rafts and comparison with postsynaptic density prepared from synaptic plasma membrane. (2010)
  • Author(s): SuzuKi, T., Zhang, J-P., Farzan, M.R., Miyazawa, S., Liu, Q., Yao W-D.
  • Organizer: APSN2010
  • Place of Presentation: Phuket, Thai
  • Year and Date: 2010-10-18
• [Presentation] Relationship between postsynaptic density and membrane rafts at the postsynaptic sites.シナプス後肥厚部-メンブランラフト複合体の解析 (2010)
  • Author(s): Suzuki, T., Zhang, J-P., Farzan, M. R., Miyazawa, S., Liu, Q., Yao W-D
  • Organizer: 神経化学/神経科学
  • Place of Presentation: Kobe
  • Year and Date: 2010-09-03
• [Presentation] シナプス後肥厚部-メンブランラフト複合体の解析 (2010)
  • Author(s): Suzuki T, Zhang J, Farzan MR, Miyazawa S, Liu Q, Yao W
  • Organizer: Neuro2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-03
• [Presentation] Mechanisms of Phosphorylation-Sensitive CaMKII Targeting (2010)
  • Author(s): KA Skelding, J Xue, T Suzuki, NM Verrills, PW Dickson, JAP Rostas
  • Organizer: Australian Neuroscience Society meeting
  • Place of Presentation: Sydney
• [Presentation] Mechanisms of Phosphorylation-Sensitive CaMKII Targeting. Australian Neuroscience Society meeting. (2010)
  • Author(s): Skelding KA, Xue J, Suzuki T, Verrills NM, Dickson PW, Rostas JAP
  • Organizer: Australian Neuroscience Society meeting
  • Place of Presentation: Sydney, Australia
• [Presentation] Proteomics analysis of lipid raft prepared from synaptic plasma membrane of rat forebrain (2009)
  • Author(s): SuzukiT , Zhang J, Farzan MR, Miyazawa S, Qian Liu, Wei-dong Yao
  • Organizer: SFN
  • Place of Presentation: Chicago
  • Year and Date: 2009-10-19
• [Presentation] Proteomics analysis of lipid raft prepared from synaptic plasma membrane of rat forebrain. (2009)
  • Author(s): Suzuki T, Zhang J, Farzan MR, Miyazawa S, Qian Liu, Wei-dong Yao
  • Organizer: Neuroscience 2009
  • Place of Presentation: Chicago, USA
  • Year and Date: 2009-10-19
• [Presentation] CaMKII Binding Partners Vary with Cell Type and Phosphorylation State (2009)
  • Author(s): Skelding KA, Verrills NM, Suzuki T, Dickson PW and Rostas JAP
  • Organizer: ISN
  • Place of Presentation: Bussan
• [Presentation] MKII Binding Partners Vary with Cell Type and Phosphorylation State. (2009)
  • Author(s): Skelding KA, Verrills NM, Suzuki T, Dickson PW, Rostas JAP
  • Organizer: International Society for Neurochemistry
  • Place of Presentation: Bussan, Korea
• [Book] Isolation of synapse-subdomains by subcellular fractionation using sucrose density gradient centrifugation (2011)
  • Author(s): Suzuki T, Li K. W.
  • Publisher: pringer Protocols Neuromethods 57
• [Book] Isolation of synapse-subdomains by subcellular fractionation using sucrose density gradient centrifugation. in Neuroproteomics in the series "Neuromethods" (Springer Protocols) (2011)
  • Author(s): Tatsuo Suzuki
  • Total Pages: 15
  • Publisher: Humana Press
• [Remarks]
  • URL: http://www.shinshu-u.ac.jp/faculty/medicine/department/doctor/grdkarei/i-kaso/index.htm