Clinical application for hepatocyte transplantation from non-heart beating donor

• Project/Area Number: 21500454
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical systems
• Research Institution: Showa University
• Principal Investigator: AOKI Takeshi 昭和大学, 医学部, 講師 (30317515)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 肝細胞移植 / 細胞凍結保存 / 臓器保存 / 温度感応生ゲル / 心停止後肝 / 冷保存 / 2層法 / 虚血再還流障害 / 臓器冷保存 / 凍結保存 / 温度感応性ゲル

Research Abstract

Liver preservation by the two layer method before hepatocyte isolation and cryopreservation of hepatocytes using thermo-reversible gelation polymer is extremely beneficial and will have an impact in the field of hepatotocyte transplantation.

Research Products

• [Journal Article] 肝臓疾患に対する再生医療の現況と展望 (2011)
  • Author(s): 青木武士、他
  • Journal Title: 昭和医学会雑誌 Volume: 71巻 Pages: 459-464
  • NAID: 130002150054
• [Journal Article] video-assisted thoracoscopic surgery : hepatectomy for liver neoplasm (2011)
  • Author(s): Aoki, T
  • Journal Title: World J.Surgery Volume: 35 Pages: 1050-1054
  • Peer Reviewed
• [Journal Article] 肝疾患に対する教室の治療戦略 (2011)
  • Author(s): 青木武士
  • Journal Title: 昭和医学会雑誌 Volume: 71 Pages: 30-36
  • NAID: 130002149947
• [Journal Article] Cold preservation of the liver with oxygeneration by a two-layer method (2009)
  • Author(s): Odaira M, Aoki T, Miyamoto Y, et al.
  • Journal Title: J Surg Res Volume: 152 Pages: 209-17
• [Journal Article] Intraoperative fluorescent imaging using indocyanine green for liver mapping and cholangiography (2009)
  • Author(s): Aoki T, Murakami M, et al.
  • Journal Title: J Hepatobiliary Pancreat Surg
  • NAID: 10027663148
• [Journal Article] Sentinel node mapping guided by indocyanine green fluorescence imaging in gastric cancer (2009)
  • Author(s): Tajima Y, Yamazaki K, Masuda Y, Kato M, Yasuda D, Aoki T, Kato T, Murakami M, Miwa M, Kusano M
  • Journal Title: Ann Surg Volume: 249(1) Pages: 58-62
• [Journal Article] Sentinel node mapping guided by indocyanine green fluorescence imaging in gastric cancer (2009)
  • Author(s): Tajima Y, Yamazaki K, Masuda Y, Koto M, Yasuda D, Aoki T, Kato T
  • Journal Title: Ann Surg 249 Pages: 58-62
  • Peer Reviewed
• [Presentation] FREE RADICAL SCAVENGER投与による冷却保存後肝組織スライス片自家移植の基礎的検討 (2011)
  • Author(s): 青木武士
  • Organizer: 日本肝臓学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-11
• [Presentation] 肝冷却保存法導入による肝組織スライス片自家移植の基礎的検討 (2011)
  • Author(s): 青木武士他
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 口演
  • Year and Date: 2011-03-01
• [Presentation] Free radical scavenger投与による冷却保存後肝組織スライス片自家移植の基礎的検討 (2011)
  • Author(s): 青木武士他
  • Organizer: 日本肝臓学会
  • Place of Presentation: 口演
• [Presentation] Cryopreserved hepatocyte transplantation from non-heart beating donor in rat Yasuda Daisuke (2009)
  • Author(s): Aoki Takeshi, et al.
  • Organizer: 36^<th> Cell Transplantation
  • Place of Presentation: Okayama
• [Presentation] Possibility of hepatocytes for cell transplantation from non heart beating donor rat liver after warm ischemia and cold preservation (2009)
  • Author(s): Aoki, Takeshi, et al.
  • Organizer: 36^<th> Cell Transplantation Okayama
  • Place of Presentation: Enami yuta
• [Presentation] 臨床肝細胞移植療法を目指した心停止後肝の有用性.新たな肝冷保存法と肝細胞凍結保存法の確立(シンポジウム) (2009)
  • Author(s): 青木武士、他
  • Organizer: 第16回肝細胞研究会
  • Place of Presentation: 山形
• [Presentation] Cryopreserved hepatocyte transplantation from non-heart beating donor in rat (2009)
  • Author(s): Yasuda Daisuke, Aoki Takeshi
  • Organizer: 36th Cell Transplantation
  • Place of Presentation: Okayama
• [Presentation] Possibility of hepatocytes for cell transplantation from non heart beating donor rat liver after warm ischemia and cold preservation, using two layer method (2009)
  • Author(s): Enami Yuta, Aoki Takeshi
  • Organizer: 36th Cell Transplantation
  • Place of Presentation: Okayama
• [Presentation] 臨床肝細胞移植療法を目指した心停止後肝の有用性-新たな肝冷保存法と肝細胞凍結保存法の確立 (2009)
  • Author(s): 青木武士, 他
  • Organizer: 第16回肝細胞研究会
  • Place of Presentation: 山形
• [Patent(Industrial Property Rights)] METHOD FOR CRYOPRESERVING MICROENCAPSULATED LIVING ANIMAL CELLS ENCLOSED IN IMMUNOISOLATION MEMBERS, SUCH MICROENCAPSULATED LIVING ANIMAL CELLS IN IMMUNOISOLATION MEMBRANES, AND BIOHYBRID ARTIFICIAL ORGAN MODULES USING SUCH MICROENCAPSULATED LIVING ANIMAL (2009)
  • Inventor(s): 青木武士
  • Industrial Property Rights Holder: 昭和大学
  • Acquisition Date: 2009-06-30
  • Overseas
• [Patent(Industrial Property Rights)] ANIMAL CELLS IN IMMUNOISOLATION (2009)
  • Inventor(s): 青木武士
  • Industrial Property Rights Holder: 昭和大学
  • Acquisition Date: 2009
  • Overseas