Development of cell microarray as devices for cell diagnosis, regulation of cell function, and recovery of target cells
| Project/Area Number |
21500457
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Medical systems
|
|---|
| Research Institution | Kinki University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMADA Yasue 近畿大学, 工学部, 教授 (00166737)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
HOSOYA Hiroshi 広島大学, 大学院・理学研究科, 教授 (90183102)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | 検査・診断システム / 細胞・組織工学 / 生体適合性材料 / 細胞アレイ / 刺激応答性材料 / 医用デバイス |
|---|
| Research Abstract |
Four types of microarrays(MA : well sizes=20, 30, 40, 50μm ; pitch=100μm) spotted with poly(N-isopropylacrylamide ; PNiPAAm) or biocompatible MPC polymers having anti-body as a specific binding for target cells were prepared by surface-initiated polymerization of NiPAAm or MPC/N-succinimidylacrylate on the glass surface of MA. Poly(MPC) was coated around spots on the MAs. Selective cell attachment was achieved by using HL60 cells or induced human pluripotent stem(iPS) cells as a typical cell on the MAs after being reacted with anti-body as a specific binding site for targeting cells. High-throughput diagnosis of attached cells on the MA was also succeeded by using developed equipments. Over 90% attached HeLa cells on the PNiPAAm coated spots of the MA were efficiently exfoliated by decreasing cell cultured temp. from 37℃to 25℃. Microcell(MC) s containing Human Artifical Chromosome(HAC) and U937 as white blood cell were higher fused on the MCs immobilized MA with a maximum of 0.1% than that of suspension cell fusion(0.001%). The MAs were of useful and for practical uses as devices for cell diagnosis, regulation of cell function, and recovery of target cells.
|
Report
(4 results)
Research Products
(48 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] 細胞診断と回収を目的としたUCSTあるいはLCST系温度応答性ポリマーを表面修飾した基板の調製と細胞の接着とはく離2012
Author(s)
伊藤大時, 朝井麻奈人, 石原達也, 今城明典, 山田康枝, 白石浩平, 杉山一男, 河済博文, 中谷達行, 岡本圭司, 新田祐樹
Organizer
第61回高分子学会年次大会
Place of Presentation
パシフィコ横浜(横浜)
Year and Date
2012-05-29
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Patent(Industrial Property Rights)] 細胞の剥離・回収及び細胞自動分取方法2011
Inventor(s)
白石浩平,杉山一男,山田康枝,河済博文,松尾理,中谷達行,岡本圭司,新田祐樹,永島正嗣,崔源煥
Industrial Property Rights Holder
学校法人近畿大学,トーヨーエイテック(株),エステック(株)
Industrial Property Number
2011-029826
Filing Date
2011-02-15
Related Report
-
-
-
-
-
-
