Changes of Transcriptome and DNA methylation in P19 neurons exposed to Bisphenol A

• Project/Area Number: 21510072
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: YAOI Takeshi 京都府立医科大学, 医学研究科, 助教 (40311914)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: 内分泌かく乱物 / エピジェネティクス / 発生・分化 / 脳・神経 / 環境化学物質 / 環境エピジェネティクス / 内分泌かく乱物質

Research Abstract

It was revealed that the exposure to one of the "non-toxic" BPA metabolites could elicit both epigenetic mutations and perturbation of transcriptome in mouse P19 neurons. The differentially expressed genes were highly significantly enriched for the BPA-responsive gene set. mRNA expression of most of them was also altered in the fetal forebrain maternally exposed to BPA. These data suggested that the metabolite was one of the potent bioactive forms of BPA molecule in the BPA-exposed fetal brain.

Research Products

• [Journal Article] The shortest isoform of dystrophin(Dp40) interacts with a group of presynaptic proteins to form a presumptive novel complex in the mouse brain (2012)
  • Author(s): Tozawa T, Itoh K, Yaoi T, Tando S, Umekage M, Dai H, Hosoi H, Fushiki S
  • Journal Title: Mol Neurobiol Volume: 45(2) Issue: 2 Pages: 287-97
  • DOI: 10.1007/s12035-012-8233-5
  • Peer Reviewed
• [Journal Article] Bisphenol A, an endocrine-disrupting chemical, and brain development (2012)
  • Author(s): Itoh K, Yaoi T, Fushiki S
  • Journal Title: Neuropathology Volume: (オンラインジャーナル) Issue: 4 Pages: 447-457
  • DOI: 10.1111/j.1440-1789.2011.01287.x
  • Peer Reviewed
• [Journal Article] Drug resistance to paclitaxel is not only associated with ABCB1 mRNA expression but also with drug accumulation in intracellular compartments in human lung cancer cell lines (2012)
  • Author(s): Shimomura M, Yaoi T, Itoh K, Kato D, Terauchi K, Shimada J, Fushiki S
  • Journal Title: Int J Oncol Volume: 40(4) Issue: 4 Pages: 995-1004
  • DOI: 10.3892/ijo.2011.1297
  • Peer Reviewed
• [Journal Article] Prenatal and Lactational Exposure to Bisphenol A in Mice Alters Expression of Genes Involved in Cortical Barrel Development without Morphological Changes (2011)
  • Author(s): Han L, Itoh K, Yaoi T, Moriwaki S, Kato S, Nakamura K, Fushiki S
  • Journal Title: Acta Histochem Cytochem Volume: 44(1) Pages: 25-33
  • NAID: 130000395313
  • URL: https://www.jstage.jst.go.jp/article/ahc/44/1/44_10042/_article
  • Peer Reviewed
• [Journal Article] Organ distribution of quantum dots after intraperitoneal administration, with special reference to area-specific distribution in the brain (2010)
  • Author(s): Kato S, Itoh K, Yaoi T, Tozawa T, Yoshikawa Y, Yasui H, Kanamura N, Hoshino A, Manabe N, Yamamoto K, Fushiki S
  • Journal Title: Nanotechnology Volume: 21(33) Issue: 33 Pages: 335103-335103
  • DOI: 10.1088/0957-4484/21/33/335103
  • Peer Reviewed
• [Journal Article] エピジェネティクスの研究方法 (2009)
  • Author(s): 矢追毅
  • Journal Title: 京府医大誌 Volume: 118 Pages: 533-541
  • URL: http://www.kpu-m.ac.jp/k/jkpum/pdf/118-8/08yaoi.pdf
  • Peer Reviewed
• [Presentation] 胎生期にBisphenol A曝露されたマウス終脳におけるDNAメチル化変動 (2009)
  • Author(s): 矢追毅, 伊東恭子, 中村惠子, 伏木信次
  • Organizer: 第3回日本エピジェネティクス研究会年会
  • Place of Presentation: 学術総合センター(東京都)
• [Remarks]
  • URL: http://www.f.kpu-m.ac.jp/k/neurpath/top.html
• [Remarks]
  • URL: http://www.f.kpu-m.ac.jp/k/neurpath/top.html