The structural determination and anti-angiogenic activity of a Chondromodulin-I subtype that lacks the N-terminal domain.
Project/Area Number |
21510224
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Living organism molecular science
|
Research Institution | Kyoto University |
Principal Investigator |
HIRAKI Yuji 京都大学, 再生医科研究所, 教授 (40144498)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 血管新生抑制因子 / コンドロモジュリン.I / 抗腫瘍血管新生因子 / 軟骨細胞外マトリックス / プロテオリシス / 活性発現の分子機構 / コンドロモジュリン-I / 細胞外マトリックス / 蛋白質分解 / 結合組織 / コンドロモジュリン-1 |
Research Abstract |
In this study, we extracted and purified the 14-kDa species of ChM-I from rat rib cartilage. Amino acid sequencing revealed a 14-kDa ChM-I lacks the N-terminal 37 residues of mature 25-kDa ChM-I. As demonstrated in vitro, 14-kDa ChM-I exhibited little anti-angiogenic activity. There is no 25-kDa ChM-I species present in the hypertrophic/calcified cartilage zone of cartilaginous bone precursors. Instead only the 14-kDa ChM-I species was detected. Taken together, our data suggest that ChM-I is inactivated by its N-terminal deletion of 37 residues during endochondral bone formation.
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Report
(4 results)
Research Products
(14 results)