Application of Multi-PK antibody that detects a wide variety of protein kinases

• Project/Area Number: 21510226
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Kagawa University
• Principal Investigator: KAMESHITA Isamu 香川大学, 農学部, 教授 (60127941)
• Co-Investigator(Kenkyū-buntansha): SUEYOSHI Noriyuki 香川大学, 農学部, 准教授 (90346635)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 生体機能関連物質 / プロテオミクス / プロテインキナーゼ / シグナル伝達 / 網羅的解析 / バイオテクノロジー / モノクローナル抗体 / キノコ / ゼブラフィッシュ / 胚発生 / インスリン分泌 / 糖尿病 / 二次元電気泳動

Research Abstract

We attempted to apply the Multi-PK antibodies for the analysis of various protein kinases. By expression screening of protein kinases using the Multi-PK antibodies, we obtained various novel protein kinases from mushroom, plant, and zebrafish, and characterized them. We also developed a new technology to identify protein kinases using the Multi-PK antibodies, and identified a protein kinase responsible for glucotoxicity in diabetis. Our present study revealed that the Multi-PK antibodies can be used as the useful tools for the analysis of various protein kinases.

Research Products

• [Journal Article] Knockdown of two splice variants of Ca^<2+>/ calmodulin-dependent protein kinase Iδcauses developmental abnormalities in zebrafish, Danio rerio (2012)
  • Author(s): Senga, Y., Nagamine, T., Kameshita, I., Sueyoshi, N.
  • Journal Title: Arch. Biochem. Biophys Volume: 517 Pages: 71-82
• [Journal Article] Knockdown to two splice variants of CaM kinase Iδ causes developmental abnormalities in zebrafish, Danio rerio (2012)
  • Author(s): 千賀由佳子, 他
  • Journal Title: Archives of Biochemistry and Biophysics Volume: 517 Pages: 71-82
  • Peer Reviewed
• [Journal Article] Functional processing of nuclear CaMKP (CaMKP-N) : evidence for a critical role of proteolytic processing in the regulation of its catalytic activity, substrate targeting in vivo (2012)
  • Author(s): 末吉紀行, 他
  • Journal Title: Archives of Biochemistry and Biophysics Volume: 517 Pages: 43-52
  • Peer Reviewed
• [Journal Article] Detection of protein kinase substrates in tissue extracts after separation by isoelectric focusing (2011)
  • Author(s): Senga, Y., Nagamine, T., Sekiguchi, M., Kaneko, K., Sueyoshi, N., Kameshita, I.
  • Journal Title: Anal. Biochem Volume: 408 Pages: 345-347
• [Journal Article] Calcium/ calmodulin-dependent protein kinase IV involvement in the pathophysiology of glucotoxicity in rat pancreatic beta-cells (2011)
  • Author(s): Sugiyama, Y., Murao, K., Imachi, H., Sueyoshi, N., Ishida, T., Kameshita, I.
  • Journal Title: Metabolism Volume: 60 Pages: 145-153
• [Journal Article] CoPK32 is a novel stress-responsive protein kinase in the mushroom Coprinopsis cinerea (2011)
  • Author(s): Kaneko, K., Sugiyama, Y., Yamada, Y., Sueyoshi, N., Watanabe, A., Asada, Y., Ishida, A., Kameshita, I.
  • Journal Title: Biochim. Biophys. Acta Volume: 1810 Pages: 620-629
• [Journal Article] Influence of Ser/ Pro-rich domain and kinase domain of doublecortin-like protein kinase on microtubule-binding activity (2011)
  • Author(s): Nagamine, T., Shimomura, S., Sueyoshi, N., Kameshita, I.
  • Journal Title: J. Biochem Volume: 149 Pages: 619-627
• [Journal Article] Application of multi-PK antibody that detects a wide variety of protein kinases (2011)
  • Author(s): 亀下勇、末吉紀行
  • Journal Title: 生化学 Volume: 83 Pages: 330-335
  • NAID: 10029068281
  • Peer Reviewed
• [Journal Article] CoPK32 is a novel stress-responsive protein kinase in the mushroom Coprinopsis cinerea (2011)
  • Author(s): 金子啓祐, 他
  • Journal Title: Biochimica Biophysica Acta Volume: 1810 Pages: 620-629
  • Peer Reviewed
• [Journal Article] Influence of Ser/Pro-rich domain and kinase domain of doublecortin-like protein kinase on microtubule-binding activity (2011)
  • Author(s): 永峰賢, 他
  • Journal Title: The Journal of Biochemistry Volume: 149 Pages: 619-627
  • Peer Reviewed
• [Journal Article] Detection of protein kinase substrates in tissue extracts after separation by isoelectric focusing (2011)
  • Author(s): 千賀由佳子, 他
  • Journal Title: Analytical Biochemistry Volume: 408 Pages: 345-347
  • Peer Reviewed
• [Journal Article] Ca2+/calmodulin-dependent protein kinase IV involvement in the pathophysiology of glucotoxicity in rat pancreatic β-cells (2011)
  • Author(s): 杉山康憲, 他
  • Journal Title: Metabolism Volume: 60 Pages: 145-153
  • Peer Reviewed
• [Journal Article] The DNA-binding activity of mouse DNA methyltransferase 1 is regulated by phosphorylation with casein kinase 1delta/ epsilon (2010)
  • Author(s): Sugiyama, Y., Hatano, N., Sueyoshi, N., Suetake, I., Tajima, S., Kinoshita, E., Kinoshita-Kikuta, E., Koike, T., Kameshita, I.
  • Journal Title: Biochem. J Volume: 427 Pages: 489-497
• [Journal Article] A minimum size homologue of Ca^<2+>/ calmodulin-dependent protein kinase IV naturally occurring in zebrafish (2010)
  • Author(s): Nimura, T., Sugiyama, Y., Sueyoshi, N., Shigeri, Y., Ishida, A., Kameshita, I.
  • Journal Title: J. Biochem Volume: 147 Pages: 857-865
  • NAID: 10027876530
• [Journal Article] Expression and characterization of PKL01, an Ndr kinase homologue in Lotus japonicus (2010)
  • Author(s): Kameshita, I., Shimomura, S., Nishio, K., Sueyoshi, N., Nishida, T., Nomura, M., Tajima, S.
  • Journal Title: J. Biochem Volume: 147 Pages: 799-807
• [Journal Article] Identification of an endogenous substrate of zebrafish doublecortin-like protein kinase using a highly active truncation mutant (2010)
  • Author(s): Shimomura, S., Nagamine, T., Hatano, N., Sueyoshi, N., Kameshita, I.
  • Journal Title: J. Biochem Volume: 147 Pages: 711-722
  • NAID: 10027875877
• [Journal Article] Oxazolone-induced over-expression of focal adhesion kinase in colonic epitherial cells of colitis mouse model (2010)
  • Author(s): Ma, Y., Semba, S., Takeuchi, M., Kameshita, I., Ishida, A., Kato, S., Katoh, T., Liu, Y., Taniguchi, T.
  • Journal Title: FEBS Lett Volume: 584 Pages: 3949-3954
• [Journal Article] Oxazolone-induced over-expression of focal adhesion kinase in colonic epithelial cells of colitis mouse model (2010)
  • Author(s): Ma Yanju, 他
  • Journal Title: FEBS Letters Volume: 584 Pages: 3949-3954
  • Peer Reviewed
• [Journal Article] A minimum size homologue of Ca2+/calmodulin-dependent protein kinase IV naturally occurring in zebrafish (2010)
  • Author(s): 二村貴樹, 他
  • Journal Title: The Journal of Biochemistry Volume: 147 Pages: 857-865
  • Peer Reviewed
• [Journal Article] Expression and characterization of PKL01, an Ndr kinase homolog in Lotus japonicus (2010)
  • Author(s): 亀下勇, 他
  • Journal Title: The Journal of Biochemistry Volume: 147 Pages: 799-807
  • NAID: 10027876295
  • Peer Reviewed
• [Journal Article] DNA-binding activity of mouse Dnmt1 is regulated by CK1 (2010)
  • Author(s): 杉山康憲, 他
  • Journal Title: Biochemical Journal 427 Pages: 489-497
  • Peer Reviewed
• [Journal Article] Identification of an endogenous substrate of zebrafish doublecortin-like protein kinase using highly active truncation mutant (2010)
  • Author(s): 下村幸子, 他
  • Journal Title: The Journal of Biochemistry 147 Pages: 711-722
  • Peer Reviewed
• [Journal Article] Novel Ca^<2+>/ calmodulin-dependent protein kinase expressed in actively growing mycelia of the basidiomycetous mushroom Coprinus cinereus (2009)
  • Author(s): Kaneko, K., Yamada, Y., Sueyoshi, N., Watanabe, A., Asada, Y., Kameshita, I.
  • Journal Title: Biochim. Biophys. Acta Volume: 1790 Pages: 71-79
• [Presentation] DCLK2の微小管結合とキナーゼ領域の核移行 (2011)
  • Author(s): 永峰賢
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] CaMキナーゼIδ-LLの生化学的諸性質の解析と遺伝子ノックダウン (2011)
  • Author(s): 千賀由佳子
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] Ca^<2+>/ CaM依存性プロテインキナーゼIδ-LL(CaMKIδ-LL)の生化学的諸性質の解析と遺伝子ノックダウン (2011)
  • Author(s): 千賀由佳子、吉岡慶子、末吉紀行、亀下勇
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Doublecortin-like protein kinase 2の微小管結合とキナーゼ領域の核移行 (2011)
  • Author(s): 永峰賢、末吉紀行、亀下勇
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] マウス脳においてタンパク質レベルと細胞内局在が日内変動するプロテインキナーゼの探索 (2011)
  • Author(s): 杉山康憲、広瀬健太郎、吉種光、亀下勇、深田吉孝
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] マウス全脳の様々な細胞内画分において発現量が日内変動するプロテインキナーゼの探索 (2011)
  • Author(s): 杉山康憲、広瀬健太郎、吉種光、亀下勇、深田吉孝
  • Organizer: 第18回日本時間生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-11-24
• [Presentation] 担子菌キノコにおける新規ストレス応答性プロテインキナーゼの機能解析 (2011)
  • Author(s): 金子啓祐、杉山康憲、山田祐介、末吉紀行、渡邊彰、麻田恭彦、石田敦彦、亀下勇
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] ゼブラフィッシュで探るCaMKPとCaMKP-Nの機能と活性調節機構 (2011)
  • Author(s): 末吉紀行
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] 植物NdrキナーゼのTyrリン酸化活性 (2011)
  • Author(s): 片山将一、杉山康憲、末吉紀行、寺地徹、亀下勇
  • Organizer: 平成23年度日本農芸化学会西日本支部・中四国支部合同大会
  • Place of Presentation: 宮崎
  • Year and Date: 2011-09-16
• [Presentation] Ca^<2+>/ CaM依存性プロテインキナーゼI(CaMKI)-deltaはゼブラフィッシュの正常な胚発生に重要である (2010)
  • Author(s): 千賀由佳子、永峰賢、二村貴樹、末吉紀行、亀下勇
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] 植物Ndrキナーゼの自己チロシンリン酸化活性 (2010)
  • Author(s): 片山将一、杉山康憲、末吉紀行、寺地徹、亀下勇
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] マウス脳におけるプロテインキナーゼの発現と細胞内局在の日内変動 (2010)
  • Author(s): 杉山康憲、広瀬健太郎、吉種光、亀下勇、深田吉孝
  • Organizer: 第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] Detection of protein kinases and phosphatases expressed in mushroom (2010)
  • Author(s): 亀下勇
  • Organizer: The Biology of Fungi-IMC9
  • Place of Presentation: エジンバラ(英国)
  • Year and Date: 2010-08-04
• [Presentation] ゼブラフィッシュの初期胚発生におけるCa^<2+>/ CaM依存性プロテインキナーゼI delta(CaMKIdelta)の役割 (2010)
  • Author(s): 千賀由佳子、永峰賢、二村貴樹、末吉紀行、亀下勇
  • Organizer: 第51回日本生化学会中国・四国支部例会
  • Place of Presentation: 山口
  • Year and Date: 2010-05-14
• [Presentation] ゼブラフィッシュDoublecortin-like protein kinase(zDCLK)の活性型変異体を用いた内在性基質の探索ならびに同定 (2009)
  • Author(s): 下村幸子、永峰賢、波多野直哉、末吉紀行、亀下勇
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] CaMKIdはゼブラフィッシュの正常な胚発生に不可欠である (2009)
  • Author(s): 千賀由佳子、永峰賢、二村貴樹、末吉紀行、亀下勇
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Doublecortin-line protein kinaseとDoublecortinの微小管結合力の違いはSP領域に起因する (2009)
  • Author(s): 永峰賢、下村幸子、末吉紀行、亀下勇
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] マウスDNAメチルトランスフェラーゼ1のDNA結合活性はカゼインキナーゼ1によるリン酸化によって制御される (2009)
  • Author(s): 杉山康憲、関口茉里、末吉紀行、波多野直哉、木下英司、木下恵美子、小池透、末武勲、田嶋正二、亀下勇
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] マウス Dnmt1のDNA結合性はCK1によるリン酸化によって制御される (2009)
  • Author(s): 杉山康憲
  • Organizer: 第82回 日本生化学会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2009-10-22
• [Presentation] 担子菌キノコに発現する浸透圧応答性プロテインキナーゼの解析 (2009)
  • Author(s): 金子啓祐、杉山康憲、末吉紀行、亀下勇
  • Organizer: 第50回日本生化学会中国・四国支部例会
  • Place of Presentation: 鳥取
  • Year and Date: 2009-05-16
• [Remarks]
  • URL: http://www.ag.kagawa-u.ac.jp/sueyoshi/index.html
• [Remarks]
  • URL: http://www.ag.kagawa-u.ac.jp/sueyoshi/index.html
• [Remarks]
  • URL: http://www.ag.kagawa-u.ac.jp/sueyoshi/index.html
• [Remarks]
  • URL: http://www.ag.kagawa-u.ac.jp/sueyoshi/index.html
• [Patent(Industrial Property Rights)] 抗チロシンキナーゼ抗体及びその利用 (2012)
  • Inventor(s): 杉山康憲、末吉紀行、亀下勇、茂里康、達吉郎、湯元昇、石田敦彦、谷口隆信
  • Industrial Property Rights Holder: 香川大学、産業技術総合研究所
  • Acquisition Date: 2012-03-30
• [Patent(Industrial Property Rights)] セリン-トレオニンタンパク質リン酸化酵素を認識するモノクローナル抗体 (2009)
  • Inventor(s): 茂里康、達吉郎、湯元昇、石田敦彦、柘植敏之、岡崎勝一郎、亀下勇
  • Industrial Property Rights Holder: 香川大学、産業技術総合研究所
  • Acquisition Date: 2009-07-17